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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 16, Issue 6 - Dec 1988
Volume 16, Issue 5 - Oct 1988
Volume 16, Issue 4 - Aug 1988
Volume 16, Issue 3 - Jun 1988
Volume 16, Issue 2 - Apr 1988
Volume 16, Issue 1 - 00 1988
Selecting the target year
Development of L-Lysine Producing Strains from Cellulosic Substrate by the Intergeneric Protoplast Fusion - Conditions for Fusion and Properties of Fusants-
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 175~181
To produce L-lysine from cellulosic substrate, the intergeneric protoplast fusion between Cellulomonas flavigena and Corynebacterium glutamicum, Cellulomonas flavigena and Brevibacterium flavum was performed. The fusion frequencies were 1.9
for the regenerated protoplasts when two parental strains were treated with 30% of polyethyleneglycol (M.W.6000) containing 5 mM EDTA at 3
for 30 min. Two fusants, FCB3 and FCC 19 were finally selected by comparision of their genetic stability and L-lysine productivity. The properties of fusants-DNA con-tent, G＋C content and L-lysine productivity-were investigated. The DNA content of fusants was greater than those of the parental strain and their G＋C contents are equal to half of total G＋C con-tent of two parental strains. The fusants showed high productivity of L-lysine from carboxy methyl cellulose as substrate.
Isolation and Characterization of Intraspecific Complementing Fusants of Penicillium verruculosum
Chung, Ki-Chul ; Park, Chang-Ryeol ; Suk Bai ; Chun, Soon-Bai ; Kim, Ki-Chung ;
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 182~186
The possibility of strain improvement of cellulolytic fungus, Penicillium verruculosum via protoplast fusion was investigated. The cellulolytic activities of the six fusants, finally selected for their hyper-cellulolytics were 2 times of those of wild type and 1.2 to 4.4 times of those parental auxotrophs. It was confirmed that the nuclear fusion occurred in fusants by their DNA contents and nuclear staining with Giemsa. It was also found that the fusants were aneuploids, and their genetic stability was demonstrated from the subculture for four months.
Microbial Conversion of Cholesterol to 4-Androstene-3,17-dione by Intermittent Addition of Substrate
Choi, S.K. ; Kim, H.S. ; Park, Y.H. ;
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 187~192
Production of 4-androstene-3,17-dione(AD) from cholesterol by microbial conversion was investigated. To facilitate the solubilization of cholesterol in the fermentation broth, ethanol was used as an organic solvent. Inhibition on cell growth by ethanol was observed to be negligible upto 2% (V/V) concentration. Microbial conversion was successfully carried out with high yield when the cholesterol was added at early logarithmic growth phase with pH control at 7.0. In order to improve the process productivity, bioconversion was conducted at various mode of cholesterol addition ; 0.1% (V/W) of cholesterol was found to be most appropriate for solubilization in ethanol and was added intermittently. When added three time(total 3 g/
), overall bioconversion yield reached upto 65% while single addition of same amount of cholesterol (3 g/
) yielded about 40% conversion.
Catalase from Aspergillus niger KUF-04
Yang, Ho-Suk ; Yang, Han-Chul ; Yoshiki Tani ;
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 193~198
Catalase from Aspergillus niger KUF-04 was purified by five steps including gel filtration. The overall purification gave 64-fold purified preparation, a yield of about nine percent. The enzyme showed its maximum absorption at 406 nm. The optimum pH and temperature for the enzyme activity were around pH 7.0 and 6
, respectively. The catalase was found to be stable in the range of pH 4.0 to pH 8.3 and temperature 2
. However, it lost nearly all of the activity by heating at 8
for 20 min. The activity was markedly inhibited by hydroxylamine, potassium cyanide and sodium azide.
Isolation and Characterization of Pseudomonas putida N3 Degrading Naphthalene
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 199~204
A strain capable of growth on naphthalene minimal medium was isolated from soil by selective enrichment culture and identified as Pseudomonas putida N3 according to its morphological and physiological characteristics. The optimum pH and temperature for growth of the isolate were 7.0 and 3
, respectively. This strain was resistant to ampicillin, chloramphenicol, kanamycin and streptomycin but. sensitive to tetracycline and rifampicin. Of the naphthalene related compounds, 1, 5-dihydroxynaphthalene was more easily utilized than naphthalene due to its solubility. And catechol was degraded through meta-cleavage pathway. A 110 Kb plasmid which encodes for a single set of enzymes responsible for the degradation of naphthalene was obtained.
Comparative Study on Continuous Ethanol Fermentation by Immobilized Tubular Fermentor
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 205~212
The immobilized cell tubular tormentor was prepared by wood chips or alginate gel. Investigations of characterization of the performance of ethanol fermentation in the immobilized cell tubular tormentor were undertaken and the results were compared with those of other tormentors. The immobilized cell tormentor packed with alginate gel showed much higher ethanol productivity than that with wood chip. It was concluded that the immobilized cell tubular tormentor packed with alginate gel might offer better perspectives for continuous ethanol production than that with wood chip.
Optimization of Simultaneous Saccharification and Fermentation of Rice Straw to Produce Butanol
Jun, Young-Sook ; Kwon, Gi-Seok ; Kim, Byung-Hong ;
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 213~218
Studies were made to optimize the simultaneous saccharification and fermentation (SSF) of rice straw to produce butanol using Clostridium acetobutylicum KCTC 1037 and a cellulolytic enzyme preparation from Trichoderma viride. The fermentation was inhibited when the liquid enzyme preparation from Novo was used, whilst a successful fermentation was achieved in the SSF using the enzyme manufactured by Pacific Chemical Co. The minimum cellulase concentration for the successful fermentation of pure cellulose was found to be 4 IU/g of substrate used. Alkaline treatment was better method for the fermentation of rice straw by the system. SSF using 25％ alkaline treated rice straw produced 150 mM butanol, 90 mM acetone. On the other hand, fermentation of ball milled rice straw was mainly acidogenic producing 98 mM acetate and 64 mM butyrate with less than 20 mM butanol. These results show that rice straw contains (a) specific inhibitor(s) for solventogenesis which is destroyed or soluble in alkali.
Purification and Characterization of Cellobiohydrolase from Trichoderma viride
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 219~225
Two isozymes of cellobiohydrolase and fifteen isozymes of endoglucanase from Trichodema viride QM 9414 were purified by ammonium sulfate fractionation, Sephadex G-100 column chromatography, DEAE-Sephadex A-50 column chromatography and preparative electrophoresis. The purified cellobiohydrolnse had a molecular weight of 71,000 estimated by electrophoresis and amino acid analysis showed its main amino acids to be in the form of aspartic acid and glutamic acid result-ing from its low pI point of 3.81. The optimum pH and temperature were 5.1 and 5
Assay of Cellobiohydrolnse by Column Single Immunodiffusion and Enzyme tinted Immunosorbent Assay
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 226~230
Antibody against cellobiohydrolase purified from Trichoderma viride had been obtained by injection to rabbit. The antibody had a high specificity against the cellobiohydroase evidienced by absence of immunological reaction to other isozymes from Trichoderma viride. Assay limit of cellobiohydrolase was 1-10
by column single immunodiffusion and by enzyme linked immunosorbent assay, it was 10-140 ng and 100-1200 pg when the dilution of antibody was 10
Studies on the Alcohol Fermentation with Extruded Tapioca Starch
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 231~237
Several methods to produce ethanol from tapioca starch were examined. Among four methods tested, alcohol fermentation with extruded tapioca starch was the most effective, which alcohol yield was 460.5 f/ton. After 69hours reaction with Rhizopus sp. glucoamylase, 108.7mg/
of reducing sugar were produced from extruded tapioca and 43.8mg/
from raw tapioca starch. In alcohol fermentation with extruded tapioca, the high concentration of alcohol at early stage prevented bacterial contamination and the fermentation rate was increased due to the high saccharifying power of glucoamylase on the extruded starch, but extrusion temperature had no influence on the fermentability, Scanning electron microscopy showed that the extrusion process changed the structure of tapioca starch granule to more susceptible form to glucoamylase attack than the raw starch. And glucoamylase of Rhizopus sp. had stronger digestion activity on both extruded tapioca and raw tapioca starch than that of Aspergillus usamii.
Regulation of Phenylalanine Specific Pathway in a Species of Intrasporangium
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 238~245
Studies were made on the regulation of chorismate mutase and prephenate dehydratase of a species of Intrasporangium, a phenylalanine producing Actinomycete isolated from soil. Two distinctly regulated species of chorismate mutase, designated CM I and CM IIwere resolved by DEAE Cellulose and DEAE Sephadex A 50 chromatography. The activity of CM II was inhibited by L-tyrosine, whereas that of CM I appeared to be unregulated. Single species of prephenate dehydyatase was also separated in the same purification steps. The activity of the enzyme was strongly feedback inhibited by L-phenylalanine, but by L-tyrosine or L-methionine it was rather slightly stimulated. Synthesis of chorismate mutase was not influenced by the presence of phenylalanine, tyrosine or tryptophan, whereas prephenate dehydratase was found to be subject to strong feedback repression by L-phenylalanine. The rate of repression was 94% at the concentration of 1mM L-phenylalanine but the repression was completely offset by the presence of 5mM tyrosine. The critical regulatory site of the phenylalanine terminal biopathway was, therefore, proved to be the second reaction which was catalyzed by the L-phenylalanine inhibitable and repressible prephenate dehydratase.
Prospectives on Mammalian Cell Culture Engineering
Lee, Hyeon Y. ;
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 246~249
Lipase-Catalyzed Reactions for Fats and Oils in Non-Polar Solvent
Daeseok Han ; Kwon, Dae-Young ; Rhee, Joon-Shick ;
Microbiology and Biotechnology Letters, volume 16, issue 3, 1988, Pages 250~258
Lipases are well known as the enzymes which catalyze the hydrolysis of ester bonds combining aliphatic chains and glycerol on mono-, di- and triglycerides. Their reactions are characterized by be-ing heterogeneous and catalyzing the water-insoluble substrates. This property has been one of the Hurdles which delayed the application of lipases in fats and oils industry, However, with the development of biological reaction system of which organic solvent is introduced in part or whole as the reaction media, enzymatic manipulation of fats and oils is attracting increasing attention from the academic and industrial sectors. Trials in two-phase system and reversed micellar system to produce fatty acids through enzymatic hydrolysis of triglycerides preyed to be efficient in respect to volumetric productivity, fat hydrolysis rate, product separation, etc. In organic solvent system lipases have been found to have the ability to catalyze aminolysis, transesterification, esterification, thiotransesterification and oximolysis that are virtually impossible to catalyze in water. The organic solvent system is being extensively used in interesterifying glycerides to produce a fat with the modified physical and chemical nature.