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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 16, Issue 6 - Dec 1988
Volume 16, Issue 5 - Oct 1988
Volume 16, Issue 4 - Aug 1988
Volume 16, Issue 3 - Jun 1988
Volume 16, Issue 2 - Apr 1988
Volume 16, Issue 1 - 00 1988
Selecting the target year
Purification of IgG1 Type Mouse Monoclonal Antibodies with DEAE-Trisacryl Chromatography
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 335~342
An anion exchange chromatography was employed for the purification of mouse monoclonal antibodies from ascitic fluid and in vitro cultivation media. After cultivation of hybridomas, Alps 25-3, HCGK, A4W, and KW, producing IgG1, the culture supernatants were harvested by centrifugation, precipitated with 50-60％ ammonium sulfate, and dialyzed against 0.025 M Tris-HCI buffer (pH 8.2). Then the dialyzed samples were loaded into a DEAE-Trisacryl M anion exchange column. Monoclonal antibodies bound to the DEAE-Trisacryl M were eluted with 0.025 M Tris-HCI buffer (pH 8.2) containing 30-40 mM NaCl. In ammonium sulfate precipitation, the recovery of the monoclonal antibody was shown to be 90％ and 84％ from in vitro culture media containing 10％ and 2％ fetal bovine serum, respectively. On the other hand, the pretreatment by ultrafiltration enhanced the yield up to 91％ whereas the purity was lower than that by ammonium sulfate treatment. Subsequently, in the DEAE-Trisacryl M chromatographic separation, the purities and recoveries of all the monoclonal antibodies from both the in vitro culture supernatants and ascitic fluids were 70-80％ and 65％ respectively. The monoclonal antibody, Alps 25-3 could be further purified with a purity of 95％ through an immunoadsorbent chromatography.
Properties of Promoters from Alkali-tolerant Bacillus sp.
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 343~347
The promoters of alkali-tolerant Bacillus sp. had been cloned in the promoter probe vector pPL703 and recombinant plasmid p-12 had been constructed. As a result of subcloning, two different promoters were found to exist in the cloned 2.9 kb promoter fragment and two recombinant plasmids p-l2B1 and p-l2B2, each harboring different promoter, were constructed. The promoter activity, which was expressed in the CAT specific activity, of p-l2B1 was 7 times higher than that of p-l2B2. The promoter activity as a function of growth revealed that both promoters of p-l2B1 and p-l2B2 were expressed after the late logarithmic growth phase and repressed in the presence of 1.0％ (w/v) glucose.
Examination of the Production of Extracellular
-Amylase by Bacillus thuringiensis, 19 serotypes
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 348~351
The production of extracellular
-amylase by Bacillus thuringiensis 19 serotypes was examined by the hydrolysis test of starch. Thirteen serotypes produced the amylase. B. thuringiensis serovar thuringiensis alesti, kurstaki, sotto, kenya, israelensis, morrisoni, entomocidus, tolworthi, thompsoni, toumanoffi, pakistani, and indiana produced tee enzyme. The amylase produced by B. thuringiensis serovar israelensis showed highest activity around pH 6.7 to 7.2 and 55
. The high production medium of the amylase was composed of 1% bactopeptone, 0.3% beef-extract, 0.3% yeast ex-tract, 0.5% NaCl, 0.3%
, 0.1% KH
, 0.2% Soluble Starch, 0.012% CaCl
, 0.005% MnCl
, and 0.03% MgCl
. The highest production of the enzyme was observed at 4 hours culture in the soluble starch (0.6 units/
) or glucose (0.43 units/
The Production of Folic Acid by Microorganisms Isolated from Fermenting Corn Meal
Yoa, Fu-Gen ; Marion L. Fields ; Hee J. Chung ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 352~357
Twenty-five out of 35 strains isolated from fermented corn meal produced folic acid. Bacillus licheniformis strain 6 and Enterobacter cloacae strain 18 produced the largest quantities of 1830
271 ng and 1350
161 ng per 100
of the assay broth, respectively. B. licheniformis produced maximum yields when initial pH values were 6,7, or 8 and were incubated at 35
for 5 days. The initial pH (range 4-8) had no effect on folic acid production by E. cloacae； 55
for 5 days was optimal for this bacterium. Added carbohydrates had no effect on the production of total folic acid in the bacterial cells in pure or mixed cultures. However, in their growth media, carbohydrates enhanced the production of free and total folic acid by E. cloacae and in the mixed cultures. Added carbohydrates had no significant (P < 0.05) effect on the production of free and total folic acid by B. licheniformis.
-Amylase using Aqueous Two-Phase System
Choi, J.S. ; Y.J. Yoo ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 358~362
Aqueous two-phase fermentation system was tested for the overproduction of extracellular enzyme through
-amylase fermentation by Bacillus amyloliquefaciens. By employing aqueous two-phase system
-amylase activity showed 25% increase compared to the result using regular medium and no deactivation of the enzyme was observed. The presence of polyethylene glycol was observed to promote the enzyme production, while to inhibit the growth of the microorganism. It is recommended that polyethylene glycol be added during the log-growth phase and dextran be added after the enzyme activity reaches Its maximum for efficient
-amylase fermentation and in situ recovery of the enzyme.
Molecular Cloning of the Gene for
-lactam Acylhydrolase from Acetobacter turbidans by Immunochemical Detection Method
Nam, Doo-Hyun ; Dewey D.Y. Ryu ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 363~368
Molecular cloning of gene for
-lactam acylhydrolase (ALAH) III from Acetobacter turbidans has been attempted by immunochemical detection method, in which polyclonal antibody from mouse Balb/c against this enzyme was employed as a probe. As a cloning vector, λ gtll was chosen for this purpose. Two positive clones has been selected from genomic libraries of A. turbidans, which had somewhat different binding affinities on anti-ALAH III umm and anti-
-galactosidase. By restriction analysis, both clones has been turned out to lose one of EeoRI sites. From these results, it concluded that deletion of DNA between lacZ gene and inserted DNA has occurred during replication of these clones in host cells.
Cloning and Expression of Thermostable Alpha-amylase Gene in Escherichia coli from Bacillus licheniformis ATCC 27811
Kim, I.C. ; S.Y. Jang ; J.H. Cha ; Y.H. Ko ; Park, K.H. ; Park, H.M. ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 369~373
The gene for thermostable alpha-amylase from the thermostable bacterium Bacillus licheniformis has been cloned and expressed in Escherichia coli. The Alpha-amylase producing E. coli cells contained a 7.4 kb chimeric plasmid (pTA 322) which was composed of the vector pBR322 and a 3.1 kb EcoRI fragment of B. licheniformis DNA. The alpha-amylase from cloned fragement was shown to be indistlnguishable from that of B. licheniformis in the optimum temperature of 9
, heat stability and the pH stability. The foreign gene was expressed efficiently in E. coli and stably maintained.
Biological Treatment of Benzene by Activated Sludge
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 374~378
Treatability and maximum no inhibitory effect concentration of benzene were measured in the synthetic wastewater medium by the activated sludge in the continuous activated sludge reactor. The maximum no inhibitory effect concentration was 1, 600mg per liter. Benzene concentration over 500mg per liter inhibited the growth of microorganims by the measurment of E/BOD, and the treatability of benzene at the maximum no inhibitory effect concentration was over 95%.
Isolation and Characterization of Benzene-degrading Bacteria.
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 379~383
To evaluate the treatability of activated sludge induced by benzene with microorganisms, isolation and characterization of benzene-degrading microorganisms were carried out. Six bacterial isolates from the activated sludge were identified ; Pseudomonas fluorescens, Enterobacter agglomerans, Enterobacter cloacae, Klebsiella oxytoca, Citrobacter freundii and Klebsiella pneumoniae. P. fluorescens degraded 55% of benzene contained in the medium as a sole carbon source, E. cloacae 24%, E. agglomerans 41%, and K. oxytoca 32%. Optimal temperature, pH and benzene concentration for growth of P. fluorescens appeared to be 31
, pH 7.0, and 300mg benzene per liter. When the P. fluorescens was dominant in the activated sludge induced by benzene, the indicator protozoa was Aspidisca sp. When concentration of benzene was about 387mg per liter, the growths of Aspidisca sp. and Litonotus sp. were high. Protozoa, Litonotus sp. and Vorticella sp. did not grow over 1600mg of benzene per liter.
Emulsification of Bunker-C Oil by a Marine Bacterium Achromobacter sp. M-1220
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 384~388
A marine bacterium Achromobacter sp. M-1220 was isolated from enrichment culture for emulsification of Bunker-C oil. The bacterium can emulsify approximately 7.5g of Bunker-C oil per liter in sen water medium within 1 drys at 18
and multiply from 8
cells to 9
cells per mi. Optimum pH and salt concentration were pH 7.5 and 3% for the emulsification of Bunker-C oil. Emulsification takes place actively in both high sulfur-containing Bunker-C oil and high sulfur-con-taming crude oil. The amount of emulsification depends on the exogenous addition of nitrogen and phosphate sources. The bacterium can also utilize n-hexndecane, n-paraffin me benzene among the petroleum compounds as a sole carbon source.
Mosquitocidal Proteins from Escheriachia coli pSL 2-1 Clone and Bacillus sphaericus 1593
Lee, Hong-Sup ; Kim, Soo-Young ; Lee, Hyung-Hoan ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 389~392
A clone pSL 2-1, which is a recombinant plasmid believed to contain the mosquitocidal crystal-line protein gene of the Bacillus sphaericus 1593, was expressed in Escherichia coli JM83 and the product of the clone was purified and identified. The unsolubilized mosquitocidal crystal proteins from the B. sphaericus had formed 43, 58, 64, 100, 113, and 130 Kd bands in the SDS-polyacrylamide gel, but the NaOH-solublized proteins at pH 12 formed 2 protein bands of 43- and 64Kd in the gel because the larger protein (precursor) bands were cleaved. The products of the pSL 2-1 clone was purified by Sephadex G-200 and only the fractions having lethal activity to the 3rd in-star larvae of mosquito Culex pipiens were analyzed by the gel. The only single protein band of 42 Kd toxic to the larvae was formed. The major toxic protein being produced from the B. sphaericus 1593 and the pSL 2-1 clone was found to be the 42 Kd.
Purification and Characteristics of Adenylate Kinase from Extreme Thermophile Thermus caldophilus GK-24
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 393~397
The adenylate kinase was purified from an extreme thermophile by adenosine-pentaphospho-adenosine elution from phosphocellulose column. The molecular weight was estimated to be 22,000 by SDS-PAGE and gel filtration. The optimum temperature of the enzyme activity was 8
and the activation energy was given as 22.4 kcal/mole. The enzyme even showed full activity after incubation at 9
or in 6M guanidine-HCI at 3
and retained 75% of its original activity even after 1 hour at 10
. The Michaelis constants of the enzymes for AMP, ADP, and ATP were 0.01mM, 0.017mM and 0.067mM, respectively.
-Lactamase Inhibitor Produced by Streptomyces sp.
Kim, J.C. ; M.Y. Kwirk ; J.S. Lee ; H.S. Lee ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 398~401
Streptomyces sp. producing
-lactamase inhibitor were isolated from soil. The culture conditions for the production of the
-lactamase inhibitor were evaluated and isolation produce of the
-lactamase inhibitor from the culture broth was also established. Some characters of the partially purified
-lactamase were determined.
Effect of Ethanol on Selected Enzymes of the Entner-Doudorff Pathway in Zymomonas mobilis
Park, I.L. ; S.H. Kwon ; Lee, K.J. ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 402~406
The aim of the presented paper was to elucidate the physiological background of ethanol inhibition on glucose uptake, ethanol production and cell growth in Z. mobilis. Data obtained from batch and continuous cultures showed that the rates of glucose uptake and ethanol production were not affected but growth rate was apparently reduced by ethanol produced. In order to know the effects of ethanol on the anabolism and the catabolism in Z. mobilis, enzyme activities of the Enter-Doudoroff pathway, viz. hexokinase, glucose 6-phosphate dehydrogenase, were analyzed with the cell grown at different concentration of ethanol produced. As results, it was found that the activities of the glucose kinase and the glucose 6-phosphate dehydrogenase were not affected greatly by the concentration of ethanol where the glucose uptake rates revealed a relatively constant value. However it was very interesting to note that transketolase, which is an essential enzyme to provide the important precursors for cell growth, was affected more apparently to reduce by increasing ethanol levels. Those results might suggest that the apparent reduction of growth rate at ethanol concentration above 20 g/
would be caused by the reduction of the transketolase activity, which in turn provide less precursor for the cell growth.
Production of L-Tryptophan by Auxotrophs Derived from Analogue- resistant Mutants of Escherichia coli
Lee, In-Young ; Kim, Jae-Hi ; Kwak, Moo-Young ; Lee, Hosull ; Lee, Sun-Bok ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 407~412
In order to increase the tryptophan productivity of E. coli SB1007, a mutant resistant to sulfanilamide was isolated and then a tyrosine auxotroph TY-90 was derived from the sulfanilamide-resistant mutant SA3-39-16. In the test-tube culture a quantitative amount of tryptophan was accumulated in strain TY-90 but in a jar fermentor culture the productivity was lower as compared to the level obtained by the parent strain. From the double auxotrophic mutant SB2756, a revertant resistant to 2, 000
-thienylalanine, TA 40-10, was selected and then phenylalanine auxotrophs were derived from the revertant strain TA-40-10. One of the phenylalanine auxotrophs, TP-4, accumulated 3.7g/
of L-tryptophan after 71-hr cultivation in a jar fermentor experiment.
Biodegradation of Aniline by Pseudomonas putida FW
Park, Y.K. ; J.S. Oh ; C.I. Ban ; S.J. Yoon ; M.S, Choi ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 413~420
The strain capable of growing on minimal medium containing aniline as a sole source of carbon was isolated from activated sludges and identified as Pseudomonas putida biotype A. The characterizations of the strain were determined. The optimum concentration for growth of the strain was 1-20 mM of aniline. No changes of pH were detected during cultivation. Some metabolic products of biodegradation of aniline were detected after cultivation of the strain on 10 mM aniline for 48 hours. The strain showed to be resistant to streptomycin, tetracycline, trimethoprim, and sulfanilamide. The strain was also capable of utilizing other aromatic compounds related to aniline as a sole source of carbon. One plasmid carried by this strain was detected. The properties of some of the mutant strains treated with mitomycin C were also discussed. The results suggest that separate, regulatory enzyme systems capable of degrading aniline may exist in plasmid DNA.
Isolation and Characterization of Aniline Degrading Bacteria
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 421~426
Twenty-nine bacterial isolates capable of growing on aniline as n sole source of carbon and nitrogen were obtained. Ten of these isolates were identified. Nine isolates were Identified as Pseudomonas spp. and one was Acinetobacter sp.. Five strains among 29 isolates had one or several plasmids. Four of these five strains utilized aniline through meta pathway and one through ortho pathway. Pseudomonas acidovorans 4A1 which utilized aniline through meta pathway harbored a plasmid of ca. 230 kilobases shown to be involved in aniline metabolism.
Effect of Dual Substrates on Aniline Mineralization by Pseudomonas testosteroni 6F1
Cho, Kyung-Yun ; Chun, Hyo-Kon ; Bae, Kyung-Sook ; Kho, Young-Hee ;
Microbiology and Biotechnology Letters, volume 16, issue 5, 1988, Pages 427~431
The simultaneons mineralization of aniline and other secondary carbon sources by Pseudomonas testosteroni 6Fl were evaluated by the lag time and the enzyme induction level. The lag time for aniline mineralization by P. testosteroni 6Fl was 7 hours, whereas the lag time for aniline and readily utilizable secondary substrates were 1-3 hours. This stimulated degradation resulted from the simultaneous use of secondary substrates and aniline, the increased rate of enzyme induction, and the in-creased rate of the cell growth. The enzyme induction level of P. testosteron 6F1 were varied according to the kinds of secondary substrate.