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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 17, Issue 6 - Dec 1989
Volume 17, Issue 5 - Oct 1989
Volume 17, Issue 4 - Aug 1989
Volume 17, Issue 3 - Jun 1989
Volume 17, Issue 2 - Apr 1989
Volume 17, Issue 1 - Feb 1989
Selecting the target year
Intergeneric Protoplast Fusion of Heterologous Transformant of Saccharomyces cerevisiae and Candida tropicalis
Seu, Jung-Hwn ; Jun, Do-Youn ; Kim, Young-Ho ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 1~7
To enhance the capability of starch fermentation of the transformant TSD-14, the heat treated protoplasts of TSD-14 were fused with the protoplasts of C. tropicalis (lys
) in the presence of 30% (w/ v) PEG and 20 mM CaC1
. Fusants were selected by nutritional complementation on minium medium and the fusion frequency was 4.4
. All fusants tested were possessed of complemented traits concerning carbon compound assimilation, and the cell volumes of the fusants were approximately 1.5 times larger than the parental strains. The fusants were genetically very stable, and were able to hydrolyze alpha 1,4-glucosidic linkage as well as alpha 1,6-linkage of starch contrary to one of parents TSD-14, The most promising fusant FSC-14-75 produced 8.7% (v/v) of ethanol from 15% liquefied potato starch medium, but the result was enhanced to 9.3% (v/v) by addition of 0.3% peptone. The corresponding fermentation efficiency was 86.0%.
Ethanol Fermentation of Fusant between Heterologous Transformant of Saccharomyces cerevisiae and Candida tropicalis in Mini-jar Fermentor Scale
Seu, Jung-Hwn ; Kim, Young-Ho ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 8~13
The optimum conditions for ethanol fermentation and ethanol productivity of the fusant ESC-14-15 were examined in a mini-jar formentor scale (working volume : 2.5 liters) to assess the possibility of practical application. Addition of yeast extract to fermentation broth greatly enhanced the ethanol productivity and shortened the period of fermentation. The pH 4.2 was more favorable than pH 5.5 with respect to ethanol productivity and fermentation speed. The optimum concentration of liquefied potato starch for ethanol fermentation of FSC-14-15 was 15%(w/v) and the corresponding productivity was 8.7%(v/v) of ethanol with an efficiency of 80.6% to the theoretical maximum. When the fresh fermentation broth containing 20% of liquefied potato starch was inoculated with love(v/v) of inoculum, the fusant FSC-14-75 produced 11.0%(v/v) of ethanol in 4 days, which is considered comparable to that from an industrial process. From the liquefied cassava starch or the equal mixture of liquefied barley and sweet potato starch prepared according to the same method as in the industrial process except saccharification step, the fusnnt FSC-14-75 produced 8.5%(v/v) or 7.6%(v/v) of ethanol in 4 days, respectively.
Ethanol Fermentation of Fusant between Heterologous Transformant of Saccharomyces cerevisiae and Candida tropicalis in Pilot Scale
Seu, Jung-Hwn ; Kim, Young-Ho ; Lee, Soon-Mo ; Bang, Byung-Ho ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 14~18
As the final experiment to assess the possibility of industrial application of FSC14-75, ethanol productivity from liquefied sweet potato starch was examined in a pilot scale of 300 liters. FSC14-75 produced 6.6％(v/v) of ethanol from 13.3％ of liquefied sweet potato starch in 8 days, and the residual sugar was 3.15％. The corresponding efficiency was 70％ of the theoretical maximum. Since we could isolate unicellular cell and flocculent cell from the fermentation broth, we designated them FSC14-75(S) and FSC14-75(F), respectively. We investigated ethanol productivity of FSC14-75(F) compared with that of FSC14-75(S) from liquefied potato starch in a mini·tar tormentor scale of 2.5 liters. FSC14-75(F) was found more favorable than the counterpart in terms of ethanol productivity, and produced 8.1％(v/v) of ethanol from 15％ of liquefied potato starch with an efficiency of 75％. In a pilot scale fermentation with 15％ of liquefied sweet potato starch, ethanol productivity of FSC14-75(F) reached maximum level of 7.7％(v/v) after 8 days, and the residual sugar was 1.9％. However, the ethanol productivity was not enhanced by a supplementary addition of Thermamyl to the fermentation broth after sterilization.
Development of Chromatographic Downstream Processing for The Purification of Monoclonal Antibody from Ascites Fluid: Part 1. Tandem Use of Hydroxylapatite Chromatography and Gel Permeation Chromatography
Ahn, I.S. ; Park, C.Y. ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 19~23
A sequential system composed of hydroxylapatite chromatography and gel permeation chromatography was developed to purify the IgM type monoclonal antibody against the colon cancer cell SC-1 from the ascitic fluid of mice injected with the murine hybridoma CH07E02. In the hydroxylapatite chromatographic step the band dilution could be reduced by controlling the gradient and flow rate of the eluent, the sodium phospate buffer, the optimum values for these variables being 5.82
M/cm and 0.2
/min, respectively. A degree of purity better than 99.99% as judged from silverstaining of the SDS-PAGE bands, was obtained by adding the gel permeation chromatographic step in tandem.
Production of Prednisolone by n Pseudo-Crystallo-Fermentation Technique : Effect of Fermentation Parameters
Chung, Bong-Hyun ; Son, Jung-Duk ; Park, Young-Hoon ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 24~28
Effect of various fermentation parameter was investigated on the production of prednisolone by microbial
'-dehydrogenation of hydrocortisone. The microbial conversion process was conducted by using pseudo-crystallo-fermentation techniques. The optimum temperature for the bioconversion process was found to be 35
. It was noted that the production rate of prednisolone was little affected within the initial pH range of 6.5-7.8, and also by the use of surfactant, Tween 80. Production rate of prednisolone was significantly reduced by the use of the antifoam agent, neolin. In a fermentor operation, however, large amount of antifoam agent should be used to remove foams generated by the high aeration rate, which resulted in n lower production rate of prednisolone than that from the shake flask experiment.
Intracellular Accumulation of Cadmium by Intact Cadmium Tolerant Yeast Cells
Yu, Tae-Shick ; Song, Hyung-Ik ; Chung, Ki-Taek ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 29~34
An intracellular accumulation of cadmium by the intact cell of an extremely cadmium tolerant yeast, Hansenula anomala B-7, was investigated in the presence of Triton X-100. The uptake of cadmium by the intact cell was efficiently enhanced up to approximately 40% or more by 0.1% of Triton X-100 and Aerosol OT, respectively. The Michaelis constant, Km, done by Lineweaver-Burk plot of accumulation velocity of cadmium vs. cadmium concentration was calculated to be 0.247mM. The optimal conditions of pH and the temperature for the effective cadmium uptake were from neutrality to alkali and 4
, respectively. The accumulation of cadmium was increased approximately 3 times under the shaking incubation, with no correlation to shaking rate. By zinc the cadmium accumulation was decreased.
Production, Purification and Characterization of
-Galactosidase from Streptococcus thermophilus 510
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 35~45
Streptococcus thermophilus 510 was investigated as n potential source of
-galactosidase. Optimum cultural conditions for maximum enzyme production were 0.5% loctose as carbon source, initial pH 7.0, 37
, and 18 hours of cultivation. The enzyme was purified to homogeneity by ammonium sulfate fractionation, protamine sulfate precipitation, Sephadex G-200 gel filtration, and DEAE-Sephadex A-50 ion exchange chromntography. The purified enzyme exhibited an optimum pH at 1.0, and an optimum temperature of 5
. Metal ions such as Mn
, dithiothreitol, and 2-mercaptoethanol stimulated
-galactosidase activity. Ethylenediamine tetraacetic add, 8-hydroxyquinoline, Hg
, and galactose were inhibitory. The
-D-galactopyranoside were 1.25mM and 88.50
moles/min.mg protein, respectively. The molecular weight was estimated to be 520,000, and the amino acid composition indicated relatively high contents of glutamic acid, aspartic acid, leucine, and valine.
Mechanism of Phosphate Regulation of Cephalosporin C Biosynthesis in Cephalosporium acremonium
Choi, Sang-Ho ; Lee, Kyoung ; Yoon, Byung-Dae ; Mheen, Tae-Ick ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 46~50
A high concentration of inorganic phosphate (above 25 mM), which was suboptimal for vegetative growth in the minimal production medium, suppressed cephalosporin C (CPC) production in Cephalosporium acremonium. Results from the determination of intracellular concentrations of ATP, ADP and AMP with phosphate-starved resting cells indicated that phosphate exerted its effect indirectly by regulating the ratio of adenylated nucleotides, the so-called adenylated energy charge. It was also found that the type of phosphate regulation of CPC biosynthesis was not a repression effect but an inhibition effect.
Conjugal Transfer of NAH, TOL, and CAM::TOL＊ Plasmid into n-Alkane Assimilating Pseudomonas putida
Kho, Yung-Hee ; Chun, Hyo-Kon ; Cho, Kyong-Yun ; Bae, Kyung-Sook ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 51~55
The conjugally transferred TOL plasmid or NAH plasmid was stably maintained and expressed in n-alkane assimilating Pseudomonas putida KCTC 2405. However, these plasmids were not able to coexist in this strain because of incompatibility. The incompatibility of TOL and NAH plasmid was bypassed using CAM::TOL* plasmid, which was constructed by the transposition of only tol gene without incompatibility system in TOL plasmid into CAM plasmid. p. putida 3SK capable of growing on m-toluate, naphthalene, camphor, and n-alkane(C8-C24) was constructed by the conjugal transfer of NAH plasmid into n-alkane assimilating p. putida SK carrying CAM:: TOL* plasmid. CAM::TOL＊ plasmid in p. putida 3SK was stable on the selective media but unstable on the nonselective media.
Isolation and Characterization of a Butyric Acid Bacterium from Infant Feces
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 56~62
To find bacteria which can inhibit growth of enteropathogenic Clostridium perfringens ATCC 13124, spore forming butyric acid bacteria were isolated from 26 fecal samples of infants. Fourteen strains were found to be antagonistic to the enteropathogen and five of them produced butyric acid. A strain which produced the highest butyric acid was selected and identified as Clostridium butyricum. This organism sporulated in SM medium in 36 hours with optimum rates at 37
and at pH 5.5. The spores tolerated well at high heat and acidity, and possible application of Clostridium butyricum as intestinal controller was discussed.
Cellular Autolysis of Clostridium butyricum ID-113
Kwag, Jong-Hui ; Lee, Se-Yong ; Kim, Tae-Han ; Lee, Jung-Chi ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 63~68
The optimum conditions for cellular autolysis in Clostridium butyricum ID-113 have been investigated. Cellular autolysis was optimal at pH 1.0 in 0.05 M potassium phosphate buffer and at 37
. The rate of cellular autolysis depended on the age of culture. The most rapid cellular autolysis occurred in the cells of mid-exponentially growing cultures, but cellular autolysis decreased sharply when the cultures entered the stationary phase. A growing culture of Cl. butyricum ID-113 was induced to autolyze and lost its turbidity spontaneously in the hypertonic NaCl, sucrose, or glucose medium. The autolytic enzyme activity was found In the autolysate of cells and the supernatant of the culture.
Some Properties of Clostridium butyricum ID-113 Autolytic enzyme
Kwag, Jong-Hui ; Lee, Se-Yong ; Kim, Tre-Han ; Lee, Jung-Chi ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 69~73
Cellular autolytic enzyme was isolated from the supernatant fluid of exponentially growing cuiture of Cl. butyricum ID-113. The autolysin was partially pruified by ammonium sulfate fractionation, chromatography on DEAE-Sephadex A-50 and gel filtration through Sephadex G-200. This autolytic enzyme lysed SDS-treated cell wall fractions of Cl. butyricum ID, but not whole cells at all. Its optimum pH and temperature were 5.0 and 37
, respectively. This enzyme was relatively stable at neutral pH, but sensitive to heat treatment. Enzyme activity was not influenced by the addition of various divalent cation, but inhibited by Cu
Hydrogen Production by the Immobilized Cells of Rhodopseudomonas sp. E15-1
Bae, Moo ; Park, Sun-Hee ;
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 74~80
For improvement of photobiological hydrogen production, Rhodopseudomonas El5-1, a photo-synthetic becterium capable of producing n high yield of hydrogen, was immobilized and conditions for hydrogen production by immobilized cells were examined. The optimum concentration for the combined matrix was obtained when sodium alginate was used at final concentration of 4%. The immobilized cells may reduce the inhibitory effects of nitrogen or oxygen. To minimize the diffusion resistance of the nutrients in alginate gel, the bend size less than 2 mm in diameter was desirable. The immobilized cells were also able to utilize n wide range of organic substrates for the production of hydrogen. The hydrogen producing activity of the immobilized cells was maintained for 20 days without loss of activity during semi-continuous operation of the reactor by feeding of new medium periodically and continuous production of hydrogen could be successfully performed for 30 days.
Immunological Variations of Flagella Antigens in Bacillus thuringiensis serovar kurstaki Temperature-sensitive Mutants
Microbiology and Biotechnology Letters, volume 17, issue 1, 1989, Pages 81~83
The flagella antigenic variation of nine Bacillus thuringiensis serovar kurstaki temperature-sensitive mutants grown at the permissive temperature (3
) was detected by a serological agglutination between H-antigen and antiserum. The flagella antigens were injected to rabbits to prepared their antisera, and then their homologous and heterologous titers of the antisera were measured. The homologous titers were ranged from 1:6,400 to 1:12,800, but the heterologous titers were very low. The H-antigen of the wild type strain was not agglutinated to 4 heterologous antisera, ts-U23 not to 7, ts-U3l not 5, ts-U32 not to 4, ts-U33 not to 7, ts-U7l not to 4, ts-U73 not to 6, ts-U74 not to 6, ts-U91 not to 4 and ts-U603 not to 4 antisera.