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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 17, Issue 6 - Dec 1989
Volume 17, Issue 5 - Oct 1989
Volume 17, Issue 4 - Aug 1989
Volume 17, Issue 3 - Jun 1989
Volume 17, Issue 2 - Apr 1989
Volume 17, Issue 1 - Feb 1989
Selecting the target year
Mass Production of Mammalian Cells under Chemostat Cultivation
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 173~177
Cell density was gradually decreased as the dilution rate was increased under chemostat cultivation of HeLa cells. Maxium cell density was maintained at the dilution rate of 0.020 (1/h) which was far less than the wash-out rate of 0.050 (1/h), Maxium cell productivity of 2 (mL of cells/L/h)was obtained at the dilution rate of 0.030 (1/h) by showing the culture also required maintenance period at low dilution rates, whose result meant the deviation of continuous culture theroy. Methods of indirectly measuring cell density have been introduced to represent mammalian cell growth, which are packed cell volume and oxygen uptake rate, and these values showed good linear relationship with actual cell density by having correlation factor of 0.90. Theoretical maximum oxygen yield,
and maintenance oxygen consumption rate, m
, were estimated as 4.1
) and 10.71
/cells/h) by employing oxygen yield model.
Molecular Cloning and Expression of a Xylanase Gene from Thermophilic Alkalophilic Bacillus sp. K-17 in Escherichia coli
Sung, Nack-Kie ; Chun, Hyo-Kon ; Shim, Ki-Hwan ; Kang, In-Soo ; Teruhiko Akiba ;
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 178~182
A gene coding for a xylanase of thermophilic alkalophilic Bacillus sp. K-17 was cloned in Escherichia coli C600 with pBR322. Plasmid pAXl13 was isolated from a transformant producing xylanase, and the xylanase gene was located in a 4.3 Kb HindIII fragment. Biotinylated pAXl13 hybridized to a 4.3 Kb HindIII fragment from chromosomal DNA of thermophilic alkalophilic Bacillus sp. K-17. The xylanase activity was observed in the extracellular curture fluid of E. coli carrying pAXl13. The pAXl13-encoded xylanase had the same enzymatic properties as those of xylanase I produced by thermophilic alkalophilic Bacillus sp. K-17.
Genetic Analysis of Caulobuter crescentus by Using Transposon Tn5 and Reverse Field Electrophoresis
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 183~187
The bacteriophage Mu and transposon Tn5 containing plasmid pJB4JI-transferred transposon Tn5 to Caulobuter crescentus. When several thousand of transposon Tn5 insertion mutants were examined, we found auxotrophic and motility mutants at frequencies of 2％ and 3％, respectively. Transposition of transposon Tn5 was analyzed by the reverse field electrophoresis and Southern hybridization. The results indicated that transposon Tn5 was randomly inserted to Caulobuter crescentus chromosome but the plasmid vector, pJB4JI, was not maintained.
Properties of Promoters Transferred to the Donor Strain, Alkali-tolerant Bacillus sp. YA-14.
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 188~192
The promoters from akali-tolerant Bacillus sp. YA-14 chromosomal DMA cloned in B. subtilis using pPL703 were stably transferred to the donor strain. In alkali-tolerant Bacillus sp., the promoters revealed similiar properties with in B. subtilis but were preyed to be more efficient than in B. subtilis comparing with pPL708. Alkali-tolerant Bacillus sp. harboring the recombinant plasmid, p-l2Bl, was abnormally more inducible with chloramphenicol than B. subtilis haying the plasmid. Therefore the host-vector system using this recombinant plasmid and alkali-tolerant Bacillus sp. was expected to be more available.
The Synthesis and Antispectrum of Alkylene Bisthioureido
-tactam Derivatives of Cefadroxil.
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 193~197
The three new Alkylene bisdithionreido
-lactam derivatives were synthesized and evaluated for antimicrobial activities. Treatment of cefadroxil.2DMF with alkylene diisothiocyanate which was obtained by the reaction of alkylene diamine with carbon disulfide, sodium hydroxide and ethyl chloroformate afforden alkylene bisdithioureido
-lactam derivatiives. The antimicrobial activities of the compounds synthesized were ethylene bisdithioureido
-lactam derivative lost the sensitivity against Escherichia coli but showed potent antimicrobial activity against
-lactams Escherichia coli, but, tetramethylene bisdithioureido
-lactam derivative and hexamethylene bisdithioureido
-lactam derivative compounds showed diminished or no antimicrobial activities.
Studies on the Production and Characteristics of Glucose Isomerase from Steptomyces sp. GI 32.
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 198~201
Steptomyces sp. GI 32 with high production of glucose isomerase was isolated from soil. The maximum enzyme production was observed in the culture medium containing 1% sorbitol, 0.6% tryptone, 0.4% yeast extract, 1mM Fe
with initial pH 7.0 when the cell was cultured at 35
about 18 hours with shaking. The enzyme was partially purified by ammonium sulfate fractionation and DEAE cellulose chromatography. The enzyme was also appeared to be relatively thermostable, and no apopreciable inactivation was observed after incubation at 7
for 1 hour. The optimal pH and temperature of the enzyme were pH 8.0 and 7
-D-Glucosidase Inhibitor from Streptomyces sp. (I) - Identification of the Strain -
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 202~206
A microorganism, YS-221-B strain which powerfully produced
-D-Glucosidase Inhibitor was isolated from soil. The morphological, cultural characteristics and physiological properties of the strain were investigated. As the results of various examinations, the strain YS-221-B was identified to be similar to Streptomyces flavouirens except the utilization of raffinose, i-inositol among the various carbon sources.
-D-Glucosidase Inhibitor from Streptomyces sp. (II) -Cultural Conditions for the Inhibitor Production-
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 207~212
Cultural conditions for
-D-Glucosidase Inhibitor production from Streptomyces sp. YS-221-B isolated from soil arid identified as Streptomyces flauovirens or a subspecies of it were investigated. When the strain was cultured in a flask containing 2% glucose, 0.3% asparagine, 0.0002% riboflavin, 0.05%
, 0.1% MgSO
, 0.05% NaCl, pH 8.0 at 3
, maximum production of the inhibitor was obtained after 8-9 days of cultivation. Sugar alcohols such as mannitol, i-inositol, erythritol as carbon sources, asparagine and beef extract as nitrogen sources were favorable for inhibitor production. Among vitamins, riboflavin, p-aminobenzoic acid, pyridoxamine and folic acid promoted the production of inhibitor, but depressed by the addition of hesperidine, and also depressed by cobalt, lithium and ferrous salts.
Conditions for Transformation of Alkalophilic Bacillus sp. K-17
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 213~218
To investigate the possibility of using alkalophilic Bacillus sp. K-11 as a host for molecular cloning, plasmid pUB110 and pBD64 were introduced into alkalophilic Bacillus sp. K-17 by protoplast transformation system. Protoplasts of Bacillus sp. K-11 were prepared by treatment with 200
Iysozyme in SMM buffer containing 0.4M sucrose. Optimal temperature, pH and culture time for protoplast formation were 4
, 7.0 and 4hrs, respectively. Cell wall was regenerated efficiently on DM3 medium containing 0.8% agar and 0.5M sodium succinate. Under these conditions for protoplast formation and regeneration, the highest transformation efficiency was obtained with cells incubated for 4hrs, and using 30%(V/V) of 40%(W/V) PEG6,000, In characteristics of transfer-mants, plasmid pUB110 was more stable than plasmid pBD64 in Bacillus sp. K-17. Maximum xylanase production of both transformants carrying pUB110 and pBD64, respectively was similar, but under the same conditions, enzyme secretion by transformant carrying pUB110 was earlier than that of transformant carrying pBD64.
Effect of Antifoam Agents on
-Dehydrogenation of Hydrocortisone
Chung, Bong-Hyun ; Son, Jung-Duk ; Park, Young-Hoon ;
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 219~223
Effect of antifoam agents, silicone oil and neolin 302, was investigated on the production of prodnisolone by microbial
-Dehydrogenation of hydrocortisone. The microbial process was conduct-ed by using a pseudo-crystallofermentation. By the hydrophobic-hydrophobic interaction, the steroid crystals aggregated with the antifoam agents. The aggregation resulted in a decrease of total mass transter area of substrate particles which is proportional to the dissolution rate of the solid substrate, and it consequently led to a significant decrease of the bioconversion rate. The bioconversion with neolin proceeded more slowly than with silicone oil. Increase of the concentration of the antifoam agents also yielded a significant decrease of the bioconversion rate.
Production of Catechol from Benzene by a Mutant of Pseudomonas sp.
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 224~230
For the production of catechol from benzene, bacteria capable of assimilating benzene as a sole carbon and energy source were isolated from soils. Among them, newly isolated strain, KY-114 hay-ing the best ability of producing catechol from benzene was selected and a mutant Pseudomonas sp. HW-103 was developed from Pseudomonas sp. KY-114 by using mutagenesis induced by N-methyl - N'- nitro - N -nitrobo guanidine. The catechol reduction from benzone by Pseudomonas sp. HW-103 was investigated under various conditions. The highest catechol concentration (0.61 g/
) was obtained in the growth medium (pH 6.5) containing 1% sodium citrate, 0.75% (NH
, 0.15% benzene and other minerals at 3
after incubating of 15hrs. In the catechol production through the reaction with resting rolls, 2.5 g/1 of catechol was produced from 4 g/
of benzene after incubation of 10 hrs under the optium conditions, which correponds to 45% of theoretical catechol yield.
Microcarrier Culture of an Anchorage-dependent Cell Using Cytodex-3
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 231~235
Possibility of using microcarriers for the growth of a transformed human embryonic kidney cell line 293 was investigated. The cell grew well in a static culture such as T-flasks with medium of DME/F12 (3:1) mixture supplemented with 5% FBS, but it was most difficult to make the cells grow on microcarriers mainly due to the low attachment efficiency and poor spreading at initial stage of the culture. Consequently, 30-50% of the cells were lost upon inoculation into microcarrier suspension and significant fraction of the mirrocarrier became bald. The medium supplemented with the concentrated conditioned medium by hepatoma cell line HpG2 supported the active growth of the cells on microcarrier and the cells showed a very healthy and well spreading morphology. It was probable that some spreading and attachment factors of HpG2 conditioned medium were effective for 293 cells.
Characterization of Pseudomonas putida 1K1 Capable of Growing on Extremely High Concentration of Toluene
Cho, Kyung-Yun ; Chun, Hyo-Kon ; Han, Dong-Cho ; Kho, Yung-Hee ;
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 236~240
The isolated bacterial strain 1K1 able to grow on extremely high concentration of toluene was morphologically and physiologically best described as Pseudomonas putida. This strain could grow on at least eight aromatic compounds, e.g., benzene, benzoate, phenol, o-cresol, m-cresol, toluene, m-tolunte, and xylene, but did not Brow on alkanes, such as hexane, octane, decane, and cyclohexane. Strain 1K1 could grow on above 95% toluene, but it could not grow on above 1% of other aromatic compounds. In the point of survival, strain 1K1 was resistant to high concentration of alkanes, appreciably resistant to toluene and xylene, and damaged by to other aromatic compounds. Strain 1K1 which grew on high concentration of toluene had irregular cell shape in comparing with normal cell shape of the genus Pseudomonas. Strain 1K1 was shown to have at least two aromatic compound dissimilation pathway, one for benzoate and the other for toluene.
Application of Computer-coupled Mass Spectrometer for Continuous On-line Monitoring of Cell Growth and Growth Rate
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 241~246
Continuous on-line monitoring of cell concentration and growth rate in aerobic batch fermentation process was carried out by analyzing the exhaust gas composition of tormentor with a quadrupole mass spectrometer. From the mass spectrometric analyses of major gaseous components, i.e.
, and the material balance equations for oxygen and carbon dioxide, oxygen uptake rate (OUR) rind carbon dioxide evolution rate (CER) were instantaneously calculated using a computer (16-bit IBM PC-AT) interfaced to a quadrupole mass spectrometer. The calculated OUR and CER data were used for the estimation of cell concentration and growth rate of Candida utilis during batch culture. It was found that the cell concentration could be satisfactorily estimated from the data of OUR arid CER during the culture and this method could be successfully und for the continuous monitoring of cell growth and growth rate.
Effects nit Mineral Salts on the Improvements of Sisomicin field
Shin, Chul-S ; Sang H. Han ; Lee, Sang H. ;
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 247~251
Effects of mineral salts on sisomicin fermentation were investigated. The optimal concentration of CoCl
for accomplishing a high antibiotic yield was found to be 16.8
M at which it could function as a cofactor. At this level the other mineral salts tested had no effect. On the other hand, at much higher concentration levels (above 1 mM), four mineral salts such as ZnSO
were used in order to liberate the intracellular sisomicin out-side the cells, because the sisomicin accumulated mostly in cells and it was supposed to limit the improvement of antibiotic yield. ZnSO
had no effect at all, and FeSO
brought about some improvement. However, by keeping the concentration of MgSO
to be 25 mM or higher in culture broths, the antibiotic yield could be improved by more than 100%, partially due to the enhanced liberation of the intracellular antibiotic.
Production of Bioemulsifier from a Marine Bacterium Achromobacter sp. M-1220
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 252~256
A marine bacterium which was isolated from the enrichment culture for the emulsification of Bunker-C oil produced a bioemulsifier potently. The strain identified as an Achromobacter sp. M-1220. The bioemulsifier was produced during mid-logarithmic phase in hexadecane oil medium at 18
. It appeared to be a cationic peptidolipid substance and showed an active stabilizing effect on the emulsion of crude oils and a few vegetable oils.
Production of Anthocyanins by Vitis Hybrid Cell Culture
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 257~262
The induction of calli from tissues of a grape, Vitis hybrid, and their suspension cultures were performed and various factors were investigated on cell growth and anthocyanin production. It was shown that light intensity and inorganic nitrogen concentration played an important role on anthocyanin production.1:he contents of anthocyanin produced under 10,000 Iux light irradiation were about twice as much as under the dark. The reduction of inorganic nitrogen concentration of MS medium to one to twenty brought about the increase of approximately five to six-fold in total anthocyanin or sixteenfold in anthocyanin content per dry cell weight and addition of nitrate only as inorganic nitrogen source was shown to be the best for anthocyanin production. Miller medium and Gamborg medium were suitable for the anthocyanin production, as well as high concentrations of Co
. And high yield of 40mg anthocyanins per 200m1 flask was obtained by two stage culture using MS medium for the first stage and the modified MS medium for the second stage.
Expression of Invertase in Recombinant Saccharomyces cerebisiae Containing SUC2 Gene
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 263~268
To maximize the performance of recombinant cell fermentation process through optimizing environmental conditions, the production of invertase from recombinant Saccharomyces cerebisiae Containing SUC2 gene was studied as a model. The recombinant cells showed biphasic growth on glucose. Since the promoter of the SUC2 is regulated by the concentration of glucose in the medium, expression of invertase by recombinant yeast began when the glucose concentration decreased in a range of 0.25-0.4 g/L during the batch culture. Plasmid segregation occured frequently during glucose fermentation, and infrequently during ethanol oxidation. A rapid appearance of invertase activity with glucose was observed under nonaerated condition, and the maximum specific invertase activity was about 1.5 times as high as under aerobic condition, In fed batch culture, when n low level of glucose was continuously supplied to the tormentor after the time of glucose depletion during growth phase, specific and total invertase activity increased about 1.7 and 2.9 fold, respectively, in a batch culture.
Development of Chromatographic Downstream Processing for the Purification of Monoclonal Antibody from Ascites Fluid: Part II Use of Single Hydroxylapatite Chromatographic Step
Ahn, I.S. ; Park, C.Y. ;
Microbiology and Biotechnology Letters, volume 17, issue 3, 1989, Pages 269~272
In order to obtain monoclonal antibody from ascites fluid at sufficiently high purity using a single hydroxylapatite chromatography (HA) a further optimization on its operating variables was carried out. By adjusting the pH of the eluent, the sodium phosphate buffer, to 6.0 from 6.8 and adding CaCl
to 1 mM at the column inlet, the elution molarities (M
) for the desired monoclonal antibody and contaminating proteins can be distinguished from each other with enough resolution. Previously these two groups of proteins co-eluted at the same time at pH 6.8 and without CaCl
. This sin81e step hydroxylapatite chromatography yields the desired antibody pure enough for diagnostic use.