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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 18, Issue 6 - Dec 1990
Volume 18, Issue 5 - Oct 1990
Volume 18, Issue 4 - Aug 1990
Volume 18, Issue 3 - Jun 1990
Volume 18, Issue 2 - Apr 1990
Volume 18, Issue 1 - Feb 1990
Selecting the target year
야생 Killer 효모 Candida dattila의 분리 및 동정
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 1~5
This study was performed to isolated woild killer yeasts which might suppress the growth of contaminant yeasts during wine making. Seventeen strains of killer yeasts which were isolated from grapes in Korea showed different killing activity; higher with K109 and K112. and lower with K117 strain. There was no inhibition among the isolates by cross-reaction. Through the physiological, morphological and cultural test, the isolates were identified as a new killer yeast, Cadida dattila, and then named Candida dattila K101-K117.
Hydrogen Evolution from Biomass-Derived Carbohydrates by Clostridia
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 6~11
Hydrogen evolution from biomass-derived carbohydrates by some Clostridia and optimal culture conditions for hydrogen evolution were investigated. Among the organisms tested, Clostridium butyricum was efficient hydrogen producer with starch, xylan, pectin, cellobiose and xylose. In batch fermentation of Cl. butyricum, optimal conditions for hydrogen evolution were achieved at pH 7.0-8.5, 10-50 mM phosphate, and 2% (w/v) glucose. Total amount of molecular hydrogen evolved by the organism slightly increased at the presence of acetate (<150 mM) or butyrate (<20 mM) in the initial fermentation medium. Especially, in case of more than the above concentration of butyrate, growth and hydrogen evolution were dramatically inhibited. In the conditions were described here, 70 mmole of molecular hydrogen per mg of DCW was produced with 1%(w/v) glucose by the organism.
Microbial Degradation of Plant Sterol to Steroidol Intermediates by a Mutant of Mycobacterium sp
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 12~17
A mutant of Mycobacterium sp. has been isolated which is capable of degrading cholesterol and plant sterol to androst-4-ene-3, 17-dione and 9-hydroxyandrostene-3, 17-dione. Also this mutant hydroxylated the steroidal nucleus at the 9
position. No ring degradation inhibitory agents are required for these processes.
Change of Paralytic Shellfish Poison Toxicity by the Treatment Method of Sea Mussel, Mytilus edulis
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 18~25
Paralytic Shellfish Poison (PSP) is mainly produced by marine dinoflagellates such as Protogonyaulax sp. and Pyrodinium sp.. The PSP was known to be accumulated in digestive gland of shellfish as result of feeding toxic dinoflagellates. PSP illness when occurs when one eats PSP intoxicated shellfish. Therefore PSP is becoming as serious problem in food hygiene and shellfish cultivation industry. The purpose of this study was to develop detoxification method for utilization of PSP intoxicated sea mussel and prevent from PSP illness. The PSP was extracted with 0.1 N HCl solution from the submitted sea mussel, then the toxicity was measured by mouse assay according to Official Methods of Analysis of the Association of Official Analytical Chemists. No detoxification effect was observed by adding extracted juice of garlic and ginger. When the sea mussel homogenate was heated at various temperatures, the PSP toxicity was not changed significantly at below
for 60 minutes but it was decreased as the heating temperature was increased. For example, when the sea mussel homogenate was heated at 100,
for 10 minutes, the toxicity was decreased about 67 and 90%, respectively. When the sea mussel containing 645
g PSP per 100g of edible meat was processed according to general shellfish canning procedure, the toxicity was decreased as the level of PSP undetected by mouse assay.
Killer Characteristics of Candida dattila KI09 and Kl12 Strains
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 26~30
Candida dattila K109 and K112 isolated from grapes in Korea showed killer activity toward Saccharomyces cereuisiae 5 x 47, S. cereuisiae 1368, Hamenula, Torulopsis, Kluyueromyces, Debaryomyces, and Brettanomyces, and showed the most effective killer activity at 22-26
and at pH 3.9-4.1. The killer actvity of both toxins were remarkably decreased at higher temperature than
and higher pH than pH 4.0. And the toxins were suggested to be glycoproteins inactivated by pronase E and pepsin. The killer activity was not cured by incubation at elevated temperature of 30-37"C, but cured by treatment with 0.0105-0.3 ppm cycloheximie.imie.
Degradation of Dibenzothiophene, and Desulfurization of Crude Oil and Bunker C Oil by Sulfate Reducing Bacteria
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 31~34
Dibenzothiophene, crude oil and bunker C oil were used in the microbial desulfurization experiments using thermophilic and mesophilic strains of Desulfovibrio and Desulfotomaculum. Mesophilic Desulforvibrio desulfuricans M6 showed the degrees of sulfur removal about 42% and 17% from dibenzothiophene and crude oil, respectively. Thermophilic Desulfovibrio thermophilus showed the degrees of sulfur removal about 68% and 33% from dibenzothiophene and bunker C oil. The strains of Desulfotomaculum were much less efficient than strains of Desulfovibrio. The latter have more complex and stronger gydrogen metabolism. These results showed that desulfurization is closely related to the hydrogen metabolism of the sulfate reducing bacteria.
Physico-Chemical Characteristics of
-D-Glucosidase Inhibitor from Streptomyces sp
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 35~38
-D-Glucosidase inhibitor purified in a pure form was amorphous powder which gave a single spot at Rf value 0.12-0.71 with various developing solvent systems on silica gel thin layer chromatography, and melting point was 154.3-155.3
. It was disolved in water, formic acid and ethylene glycol monoethyl ether, and was very high hygroscopic substance. Biochemical reaction of the substance was positive to phenol sulfuric acid, ninhydrin, silver nitrate-sodium hydroxide, but negative to DNS reagent. Acid hydrolysis gave fructose and acid as sole sugar and amino acid constituents respectively. Moelcular weight of the inhibitor was estimated to be 1,050 by Shphadex G-25 column chromatography.
Inhibition Mechanism of
-D-Glucosidase Inhibitor from Streptomyces sp
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 39~43
The inhibitor had the inhibitory activities against hydrolysis of PNPG, sucrose and ONPG by
-galactosidase, but it did not inhibit amylases and other carbohydrases. Kinetic studies exhibited that the inhibitory substance non-competitively inhibited the enzyme reaction with a Ki value of 118
, and enzyme-inhibitor complex was formed slowly.
Purification and Characterization of Cyclodextrin Glycosyltransferase from Alkalophilic Bacillus sp
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 44~48
Alkalophilic sp. YC-335 isolated from soil was capable of producing large amount of cyclodextrin glycosyltransferase (CGTase) in culture broth. This enzyme was successively purified 52.9 folds with 17.8 yield by ethanol precipitation, DEAE-Toyopearl column chromatography and Sephadex G-100 column chromatography. The purified enzyme have a molecular weight of approximately 75,000 estimated by SDS polyaerylamide gel electrophoresis. The optimum pH and temperature for the enzyme activity were 6.0 and 5
, respectively. The enzyme stable between pH 6 and 10, and up to 5
. The thermostability of the enzyme was increased up to 6
by the addition of 15mM CaCl
Enzymatic Characteristics in the Bioconversion of D,L-ATC to L-Cysteine
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 49~55
The bioconversion of D, L-20aminothiazoline-4-carboxylic acid (D, L-ATC) to L-cysteine was investigated. After the intracelluar enzyme of a Pseudomonas species was inducibly formed by addition of D, L-ATC in the middle of culture, the cells were isolated and treated with sonication to prepare the crude enzyme solution. The results indicated that the cysteine was produced only in the form of L-isomer from D,L-ATC and its production could be enhanced several tens times by addition of managanese ions which were required as a cofactor in this enzymatic reaction. Bedies, this reaction suffered from the feedback inhibition of L-cysteine. On the other hand, since L-cysteine-decomposing enzyme coexisted in the crude enzyme solution, most of the L-cyseine formed disappeared in the absence of its inhibitor. However, hydroxylamine was found to be a potent inhibitor which could successfully prevent the decomposition of L-cyseine.
A Study on Stability of Nitrile Hydratase of Brevibacterium sp. CHI Under the Various Conditions
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 56~60
A bacterial strain of Brevibacterium sp. CHI was isolated from soil and used to produce an enzyme (nitrile hydratase) necessary for carrying out the bioconversion of acrylonitrile to acrylamide. Various immobilization methods and enzyme stability were investigated. The nitrile hydratase showed the maximum stability at pH 7 for the free cells. EDTA and phenyl methyl sulfonyl fluoride were selected as the protease inhibitor and the enzyme stability was evaluated by changing inhibitor concentration. Acrylamide beads were the best carriers among four carriers we tested in terms of stability and physicoehemical strength. The storage stability of the immobilized cells decreased with increasing acrylamide concentration of the gel phase at 4
, and was very low at acrylarnide concentration above 25%.
Pulse-Feeding of Serum Free Media for Enhancing Monoclonal Antibody Production under Perfusion Operation
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 61~65
Lectin related inducer can enhance IgG
production rate from murine hybridoma cells by employing step-feeding of serum free media with producing about 40 mg/
of monoclonal antibodies. This step-feeding perfusion process also proves to be able to cutivate animal cells when serum free media can not support the growth of these cells in perfusion process, as well as to improve production rate. This process yields about 28 x 10
mg of MAb/cells/h compared to 11.1 x 10
and 4.0 x 10
mg/cells/h for perfusion process and batch cultivation with 10% serum containing media, respectively.
Biotransformation of Progesterone to 11
-Hydroxyprogesterone by using Rhizopus nigricans at Elevated Concentration of the Substrate
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 66~70
A study on 11
-hydroxylation of progesterone by using Rhizopus nigricans was carried out to produce efficiently 11
-hydroxyprogesterone which is an essential intermediate of corticosteroids synthesis. Firstly, medium was optimized in view of bioconversion yield and cell growth. Glucose and casamino acid were selected as carbon and nitrogen source and the ratio of carbon to nitrogen which maximize bioconversion yield was determined to be 2:1. Secondly, the addition time of progesterone and dispersion method were studied. When progesterone dispersed with 0.01% (v/v) Tween 80 was added at 12-14 hr of cultivation, higher bioconversion yield was obtained. When 20g/
of progesterone was added, the yield reached 70% under optimized conditions.
Studies on the Preparation of Fermented Milk by Bifidobacterium longum and Lactobacillus acidophilus
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 71~75
Yoghurt was prepared with Bifidobacterium longum TK-100 and Lactobacillus acidophilus TK-2070. The prepared yoghurt showed the increase of the titratable acidity under cold storage condition. This was derived from the active L. acidophilus TK-2070 on the logarithmic phase rather than from the B. longumn TK-2070. B. longum TK-100 grew well in the facultative anaerobic condition as well as in the strict anaerobic condition. Reinforced clostridial agar medium with 0.1% aniline blue was tried for the differential viable cell counts in the mixed culture and in the yoghurt. B. longurn TK-2070 had the light gray, blue-dotted colonies of about 2 mm diameter. L. acidophilus TK-2070 had the light gray colonies of about 1 mm diameter.
Production of Soymilk Clotting Enzyme by Bacillus lichenifQrmis
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 76~80
The production of extracellular soymilk clotting enzyme by Bacillus licheniformis strain 192, one of the soymilk clotting enzyme producers isolated formerly, was studied under various conditions. The medium composed of 1.5% potato starch, 2.0% soybean milk, 10% defatted soybean meal extract and 0.6% KH
(pH 6.1) was chosen as the most suitable medium and the culture at 35-4
for 3 days was most appropriate for the production of clotting enzyme.
Antifungal Mechanism of Pseudomonas stutzeri YPL-l for Biocontrol of Fusarium solani causing Plant Root Rot
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 81~88
For the selection of powerful antagonistic bacterium for biological control of soilborne Fusarium solani causing root rot of many important crops, the best YPL-1 strain was selected among 300 strains of bacteria isolated from rhizosphere in ginseng root rot-suppressive soil. The strain was identified to be a species to Pseudomonas stutzeri. With in vitro fungal inhibition tests, antagonistic substance of P. stutzeri YPL-1 against F. solani was presumed to be heat unstable, macromolecular substances such as protein. Also, it was shown that antifungal activity of P. stutzeri YPL-1 increased in proportion to its chitinase production. P. stutzeri YPL-M122 (chi-, lam -) which was deprived of the productivity of chitinase and laminarinase by NTG mutagenesis had lost antifungal activity, completely. And P. stutzeri YPL-MI53 (chi-) had only 4.1% of its antifungal activity. P. stutzeri YPL-1 was not able to produce any extracellular siderophore in iron-deficent minimal medium. It is confident that the antifungal mechanism of P. stutzeri YPL-1 for biocontrol of F. solani depends on lysis rather than antibiosis :the mechanism of lysis appears to involve enzymatic degradation of the cell will components of F. solani by hydrolytic enzymes of more chitinase and less laminarinase.
Stable Maintenance of Recombinant Plasmid Containing trp
Operon in E. coli Cultures by the phe W
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 89~93
To improve the stability of recombinant pBR322-trip
plasmid (pLTW24) in E. coli culture, a positive selection system was devised. A DNA fragment containing pheW
gene (a structural gene for tRNA
was isolated and inserted into the pBR322-trip
plasmid(pLTP24). A temperature sensitive host strain. LC901-pheS
, was constructed for this plasmid by transducing pheS
allele (phenylalanyl-tRNA synthetase) to E. coli LC901 using P1kc bacteriophage. The LC901-pheS
cells were unable to grow at a restrictive temperature when they had lost the pBR322 :: pheW
(pLTP24) plasmid. The effects of pheW
gene on the plasmid stability and the expression level of trip
gene in LC901-pheS
strain were investigated. The proportion of Trip
colonies among LC901-pheS
strain carrying plasmid pLTP24 was 99%, whereas that of LC901 strain carrying plasmid pLTW24 was 7% at the end of 20 generations. After 100 generations of growth, the strain LC901-pheS
carrying plasmid pLTP24 showed little loss of plasmids. While the majority of plasmid pLTW24 in LC901 strain were lost in the same period. The activities of tryptophan synthetase (T. Sase) and anthranilate synthetase (A. Sase) in LC901 strain carrying pLTW24 were about 1.2 times and 1.8 times respectively of those in LC901-pheS
strain carrying pLTP24.
Properties of Recombinant Derivatives of pJY501, A Multi-copy Streptomyces plasmid
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 94~97
The restriction cleavage map of multi-copy recombinant plasmid, pJY502 (5.5 kb), carrying the thiostrepton resistance gene (tsr) was determined. Comparison of the restriction pattern with that of Streptomyces plasmids previously demonstrated that pJY502 was novel. The plasmid pJY502 had a broad host range in Streptomyces and contained single BgtII site for cloning purpose. Transformation frequency of pJY502 was
in S. lividans. E. coti-Streptomyces bifunctional plasmid, pJY504, was also constructed.
Molecular Cloning of Bacteriocin Gene and Biological Control of Plant Pathogen
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 98~102
A strain of Erwinia spp. was selected from the soil for the production of bacteriocin to the root rot plant pathogen. Bacteriocin producing gene was not located on plasmid but on chromosome. Genomic library of Erwinia spp. were made by using pLAFR 3 as a vector system for cloning of the gene. It was been cloned and expressed in E. coli DH 5 . Bacteriocin producing colony was composed of pLAFR 3 vector and 3.0 kb EcoRI fragment of Erwinia spp. ehromosomal DNA. The inserted fragment (3.0 kb) was possessed a EcoRI and BarnHI restriction sites.
Enzymatic and Genetic Aspects of Glyoxalase I in Microorganisms
Microbiology and Biotechnology Letters, volume 18, issue 1, 1990, Pages 103~108
The enzymatic studies on the methylglyoxal metabolism in yeast and bacterial cells indicated that organisms are equipped with the common and manifold systems for the detoxification of methylglyoxal. Among these systems, the glyoxalase I is the most important route for methylglyoxal detoxification. The molecular structure of glyoxalase I is apparently distinct from the enzyme sources, and zinc ion is an essential cofactor in enzyme activity. The gene for Pseudomonas putida glyoxalase I functioned as a scavenger of methylglyoxal and regulated the cell size of the bacterium. Comparison of the nucleotide sequence of the P. putida glyoxalase I gene with the N-terminal amino acid sequence of the purified enzyme revealed that the N-terminal methionine residue was removed after translation. Possible physiological role of glyoxalase I was also discussed.