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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 18, Issue 6 - Dec 1990
Volume 18, Issue 5 - Oct 1990
Volume 18, Issue 4 - Aug 1990
Volume 18, Issue 3 - Jun 1990
Volume 18, Issue 2 - Apr 1990
Volume 18, Issue 1 - Feb 1990
Selecting the target year
Studies on Protoplast Formation and Regeneration of Bacillus pumilus and Cellulomonas fimi
Kim, Do-Man ; Lee, Ke-Ho ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 109~109
Several factors predicted to affect the protoplast formation and regeneration were investigated. The optimum concentration of lysozyme, casamino acid and polyvinylpyrrolidone were 0.5 (mg/ml), 0.1%, and 1.5% respectively. In Bacillus pumilus, Penicillin-G treatment concentration was 0.3 U/ml and optimum treatment period was transit log. phase. And in the case of Cellulomonas fimi, 0.3 U/ml and initial log. phase. Osmotic stabilizer and di-cation for OSM medium for Bacillus pumilus and Cellulomonas fimi were 25 mM
0.5 M sodium succinate and 50 mM
0.4 M sodium succinate. The regeneration frequency of Bacillus pumilus and Cellulomonas fimi were 14.6% and 6.9% respectively.
Development of Extracellular
-glucosidase Producing Strains by Intergeneric Protoplast-fusion between Bacillus pumilus and Cellulomonas fimi
Kim, Do-Man ; Lee, Ke-Ho ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 115~115
Cellulose utilizing hybrids between Cellulomonas fimi and Bacillus pumilus were isolated after polyethyleneglycol (PEG) mediated protoplast fusion. 33% (w/v) PEG #6,000 and 50 mM
were optimum concentration. The intergeneric fusion frequency was
Extracellular CMCase and
-glucosidase activities were detected from one hybrid unlike only CMCase was detected from Cellulomonas fimi.
Protoplast Fusion between Candida dattila K109 and Wing Yeast
Chung, Won-Chul ; Choi, Eon-Ho ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 121~121
In order to introduce a killer factor of Candida dattila K109 isolated from Korean grapes to Saccharomyces cerevisiae M524, a wine making strain, the protoplast fusion between these two yeast strains was investigated. The cell walls were lysed by treatment of zymolyase 60,000 at the concentration of 0.02% for 4 hr for candida dattila K109 and at 0.01% for 2 hr for S. cerevisiae
And then two fusants were obtained at a frequency of
and named K109F1 and K109F2. In mixed culture, the fusants inhibited the growth of S. cerevisiae
known as a killer sensitive strain to reduce the population into 0.003% of total cell number and to show 0.084g/l/day of productivity of ethanol after 4-days cultivation. The killing activity of fusant was remarkablly observed at even pH 3.5 lower than pH 4.0, limiting pH of most killer yeasts.
Stability of Clostridial Spore in Soil Culture and Screening for Plasmids in the Clostridium strains
Yoon, Ki-Hong ; Lee, Jung-Kee ; Kim, Byung-Hong ; So, Sun-Joo ; Chun, Yung-Sook ; Kwon, Gi-Seok ; Zeikus, Z.G. ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 126~126
Stock cultures of Clostridim, which had been maintained as spores with soil as carrier for 50-70 years, were used to determine their revival ratio, and plasmids were screened among the revived strains. From 131 soil cultures, 100 strains grew in the liguid medium giving the revival ratio of 76%. Analyses of the fermentation products divided the revived strains into 63 solventogenic strains and 37 acidogenic strains. Agarose gel electrophoresis showed one or more plasmid bands in the cleared lysates of 50 revived cultures. The sizes of the plasmids range in size from 1 to more than 50 kilobase pairs.
Production of Apple-like Aroma by Geotrichum sp. Isolated from Sludge
Kim, Dong-Wook ; Kim, Hyean-Wee ; Lee, Byung-Woo ; Chung, Chang-Ki ; Park, Ki-Moon ; Sung, Ha-Chin ; Choi, Chun-Un ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 132~132
A strain producing apple-like aroma was isolated from sludge and identified as Geotrichum sp. Optimum culture conditions for the aroma production were investigated and the major aroma components were identified by GS and GC-MS. The ratio of leucine to phenylalanine was 100:1 (1.5 g/l) and ethanol concentration was 0.5-1.0% (v/v). The best aroma quality was obtained at
and pH 4.0. Major aroma compounds were alcohols including 3-methylbutyl alcohol, phenetyl alcohol, 2-hexene-1-ol and their esters, and esters originated from ethyl alchol.
Effects of Glucose and Chloramphenicol on the Expression of Promoter from Alkali-Tolerant Bacillus sp. chromosomal DNA
Kim, In-Gyu ; Lee, Seok-Hoon ; Cho, Hyung-Yong ; Pyun, Yu-Ryang ; Yu, Ju-Hyun ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 137~137
The promoter from alkali-tolerant Bacillus sp. YA-14 chromosomal DNA is unexpressed in vegetatively growing cells under excess glucose. After glucose in the medium consumed nearly completely, the activity of the inserted promoter was dramatically increased, and its maximum activity was attained in the initial stage of sporulation. The plasmid cloned in Bacillus sp. YA-14 was stable for 40 hours. The optimal initial concentrations of glucose and chloramphenicol were 1 g/l and 10
g/ml, respectively for the maximum expression of the promoter.
Antifungal Antibiotic Against Fruit Rot Disease of Red Pepper from Streptomyces parvullus
Lee, In-Kyoung ; Kim, Chang-Jin ; Kim, Shin-Duk ; Yoo, Ick-Dong ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 142~142
A strain producing active compound against Phytophthora capsici and Phytophthora parasitica was selected from 4,087 strains of Actinomycetes isolated from domestic soil. This strain was identified as Streptomyces parvullus by the cultural, morphological and physiological characteristics. The active compound was purified from culture broth by ion-exchange, adsorption, gel permeation and partition column chromatography and identified as polyoxin by the physicochemical and biological properties. This is the first report that polyoxin is active against fruit rot disease of red pepper and is positive antagonizer of detoxin to Phytophthora capsici and Pyricularia oryzae.
Preparation of Plant Cell Wall Lytic Enzymes from Fusarium moniliforme and Protoplast Isolation from Plant Leaf and Cell Suspension Cultures
Chung, Sei-Hoon ; Park, Kwan-Hwa ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 148~148
Culture conditions of Fusarium moniliforme for the simultaneous production of cellulolytic enzymes and pectolytic enzymes for plant cell wall lysis and their capability of plant cell wall degradation were investigated. Plant cell wall lytic enzymes with relatively high activity were obtained in liquid medium containing 1.0% microcrystalline cellulose and 0.6% citrus pectin as inducible carbon sources. Although there was a time discrepancy between the production cellulases and pectolytic enzymes, 4-day of culture was optimum for producing both enzymes with maximum potency of cell wall lytic activity. The results of the lysis of leaf mesophyll tissue and cell suspension cultures of some crop plants indicate that the lytic enzyme preparation was comparable with commercial enzymes in the aspects of protoplast yields and its applicability.
Purification and Characterization of Plant Cell Wall Lytic Enzyme from Fusarium moniliforme
Chung, Sei-Hoon ; Park, Kwan-Hwa ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 154~154
Cultures of Fusarium moniliforme were induced to secrete plant cell wall degrading enzyme. The major constituents of plant cell lytic enzyme, cellulases and endo-polygalacturonase in culture supernatant were separated by
fractionation first and each enzyme was further purified by Sephadex G-75, DEAE Sephadex A-50 and Sephadex G-100, respectively. Action pattern on plant cell wall lysis and some properties of these enzyme fractions were also studied. The results suggest that both
components of cellulases and macerating activity of endo-PG cooperatively act on the plant cell wall lysis.
Properties of Alkaline Protease Produced by an Alkalophilic Bacillus sp.
Kim, Tae-Ho ; Park, Sung-Hee ; Lee, Dong-Sun ; Kwon, Taeg-Kyu ; Kim, Jong-Kuk ; Hong, Soon-Duck ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 159~159
A bacterial strain no. K-9, which was capable of producing alkaline protease in the alkaline culture conditions, was isolated from soil. The isolated strain was identified to belong to the genus Bacillus. The range of pH and temperature for growth was between 8 and 11.5, and up to
respectively. The enzyme was purified about 20 folds with 12% yields by the following continous treatment of 60% ammonium sulfate precipitation, DEAE-cellulose, Bio-gel P-150 chromatography. The optimal pH and temperature of the enzyme was 10 and
respectively. The stable range of pH was from 8 to 11. Enzyme activity was lost up to 40% on heating at
for 30 minutes. The activation energy calculated by Arrhenius equation was 12.15 kcal/mole, and
value for casein was 10 mg/ml.
among metal ions inhibited the enzyme in the final concentration of 1 mM, but
promoted at 1 mM.
Purification of Extracellular Protease from Serratia marcescens
Kim, Nam-Soo ; Nam, Young-Jung ; Kim, Su-Il ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 165~165
Serratia marcescens ATCC 25419 produced an extracellular protease. Among the nutrient additives tested, only casein increased enzyme production slightly. Protease production was found to be growth-dissociated type, with maximum enzyme productivity of 106.2 U/ml after 24 hr culture. Through the
treatment, Sephadex G-100 gel filtration, DEAE-Sephadex A-50 ion exchange chromatography, and Sephadex G-75 gel filtration, specific activity of the extracellular protease increased from 2.92 U/mg protein to 1119.11 U/mg protein. Single protein band with relative mobility of 0.24 was observed after polyacrylamide disc-gel electrophoresis. This protein band was active, when confirmed by gel slicing technique.
Effects of Cultural Conditions on the Production and Characteristics of Unsaponifiable Lipids of Rhodotorula glutinis
Lee, Kun ; Yoon, Suk-Hoo ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 171~171
A red oleaginous yeast, Rhodotorula glutinis, was cultured in nitrogen- and carbon-limited conditions, lipogenic and non-lipogenic conditions, respectively, to produce microbial lipids. The unsaponifiable lipids in the total lipids were sterols, carotenoids and coenzyme Q. Ergosterol, episterol, zymosterol and ergosttrienol were found to be present in Rhodotorula glutinis. Such pigments as torulahodin, torulene, neurosporene, beta- and gamma-carotenes were also detected in R. glutinis.
Production of Anti-Hepatitis B Surface Antigen Monoclonal Antibody in Serum-Free Medium
Chun, Bok-Hwan ; Jo, Eui-Cheol ; Kim, Dong-Il ; Paik, Sung-Bok ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 175~175
For the large-scale cultivation of murine hybridoma 2c3.1 cells secreting anti-Hepatitis B surface antigen (anti-HBsAg) monoclonal antibody (MAb), we have constructed a serum-free medium. The serum-free medium was supplemented with human serum albumin, insulin, transferrin, fat emulsion
soybean oil, ovolecithin, glycerin), vitamin E, vitamin E acetate, and monoethanolamine in a mixture of RPMI 1640 and Ham's F12 medium. The cells in serum-free medium propagated logarithmically without lag period, and the maximum concentration of anti-HBsAg monoclonal antibodies secreted by 2c3.1 cells was higher than that in 10% fetal bovine serum (FBS) medium. The serum-free medium was enough to substitute 10% FBS medium in respect of MAb production.
Desorption, Recovery and Utilization of Spent Cellulase used for Enzymatic Hydrolysis of Cellulose
Park, Jin-Seo ; Lee, Yong-Hyun ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 181~181
To enhance the process economics for enzymatic hydrolysis of cellulose, the recovery and reuse of spent cellulase is essential because of high cost of enzyme. The various desorption methods for recovery of adsorbed spent cellulase on undigested cellulose were compared. The maximum desorption, with recovery yield of 62.2% of soluble protein, 62% of carboxymethyl cellulase (CMCase) activity, and 44% of filter paper (FPase) activity, was achieved by treating with pH 10 glycine-NaOH solution at
in the presence of 0.1% Tween 80. On the other hand, around 87% of the free spent cellulase contained in hydrolysate was successively recovered by adsorption on fresh substrate and then filtration with sinter glass. Total recrvery yield of spent cellulase, around 70% of soluble protein, 57% of filter paper (FPase) activity, and 65% of carboxymethyl cellulase (CMCase) activity, was achieved by desorption and readsorption on fresh substrate.
The Role of Chitinase of Pseudomonas stutzeri YPL-1 in Biocontrol of Fusarium solani
Lim, Ho-Seong ; Kim, Sang-Dal ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 188~188
Pseudomonas stutzeri YPL-1 produced extracellular chitinase and
-(1,3)glucanase as a factor of biocontrol, which are key enzymes in the lysis of fungal cell walls, when grown on different polymers, such as chitin, laminarin, or Fusarium solani mycelium as a carbon source. The lytic extracellular enzymes were able to degrade F. solani cell walls. The germination of chlamydospore was not affected by culture filterate of P. stutzeri YPL-1 on the PDA disc after 24 hr incubation, but the growth of germ tube was inhibited, about 97.7% of the germ tube appeared to be undergoing lysis. Degradation of hyphae of F. solani was observed by scanning electron microscopy. Abnormal swelling and retreating of hyphae was observed. Lysed holes were observed on the hyphae in the regions of interactions with the P. stutzeri YPL-1. The walls of these regions were rapidly lysed, leaving remnants of the protoplasm. These results suggest that chitinase and
-(1,3)glucanase produced by P. stutzeri YPL-1 attack these sites and completely degrades the hyphae in lytic mechanism for biological control of the plant pathogenic fungus F. solani.
Recombinant Plasmid DNA containing Xylanase and
-Xylosidase Gene of Bacillus sp. YA-14
Na, Kyu-Heum ; Kim, Jin-Man ; Park, Hee-Kyoung ; Bai, Dong-Hoon ; Yu, Ju-Hyun ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 195~195
A recombinant plasmid, pYDX43, was constructed by inserting 5.7 kb EcoRI DNA fragment of pYXL22 containing
-xylosidase gene into EcoRI site of pYDC21 which was carrying encoding xylanase gene. The end-product of xylan hydrolysis with crude cell extract of E. coli HB101 harboring pYDX43 was found to be xylose by TLC analysis. The productivity of xylanase with E. coli HB101 (pYDX43) was 1.3 times higher than that with Bacillus sp. YA-14, but the synthesis of
-xylosidase was rather 2.5 times lower.
Construction of a Novel Shuttle Plasmid Vector pHN114
Park, Sung-Hee ; Kwon, Taeg-Kyu ; Kim, Jong-Kuk ; Hong, Soon-Duck ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 199~199
We have constructed a chimeric plasmid vector, pHN114, from the Escherichia coli vector pUC19 (2.7 kb) and the yeast vector YRp7 (5.8 kb). Plasmid pHN114 was composed of TRP1 and ARS of YRp7, and
ori and multicloning site of pUC19. It was about 4.15 length. The plasmid showed high transformation efficiency and stability in both host cells, Saccharomyces cerevisiae and Escherichia coli. The plasmid is a useful shuttle vector functioning in S. cerevisiae and E. coli. In addition, plasmid pHN114 has unique sites for SacⅠ, KpnⅠ, SmaⅠ, BamHⅠ, SalⅠ and SphⅠ.
Molecular Cloning of the Cell-Wall Degrading Enzyme in Rhizobium
Yun, Han-Dae ; Lim, Sun-Teak ; Kang, Kyu-Young ;
Microbiology and Biotechnology Letters, volume 18, issue 2, 1990, Pages 203~203
Rhizobium fredii USDA193 infects and nodulates soybean root via root hairs by actively penetrating the root hair cell-wall. The penetration of Rhizobium into root hair cell-wall requires the activity of cell-wall hydrolyzing enzymes such as cellulases. We constructed a genomic library by cloning Sau 3A-digested genomic DNA from R. fredii USDA193 DNA into the BamHI site of the cosmid vector
Out of 1500 Tc-resistance transductants of E. coli, one clone(YA17) had cellulase activity and contained
cosmid with 27 kb insert of R. fredii DNA. A 27 kb BamHI fragment coding for cellulase was subcloned in pUC18 vector and a physical map of the resulting plasmid YA100 was constructed. Finally, we have subcloned 1.7 kb Bgl II-HincII fragment DNA coding for cellulase in pUC18 (YA300). The expression of rhizobial cellulase gene in E. coli was studied and compared with the products of cellulase genes from Clostridium thermocellum in E. coli.