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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 18, Issue 6 - Dec 1990
Volume 18, Issue 5 - Oct 1990
Volume 18, Issue 4 - Aug 1990
Volume 18, Issue 3 - Jun 1990
Volume 18, Issue 2 - Apr 1990
Volume 18, Issue 1 - Feb 1990
Selecting the target year
Isolation and Identification of Streptomyces californicus KS-89 Produced Bluish Purple Pigment
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 443~448
The objective intended for this study is that of providing a fairly practical guide to the use of natural pigment in the food industry. Streptomyces isolated from soil were carried out test for the excretion of their bluish purple pigment. One strain of Streptomyces, strain KS-89 showed a high production of bluish purple pigment on the glycerol starch-glutamate medium. The morphological and physiological characteristics of the strain KS-89 were studied according to the methods of Bergey's manual, Nonomura's classification, and Ridham and Lyons classification. Based on the results obtained in these experiments, strain KS-89 was identified as Streptomyces californicus.
Isolation and Identification of Mucor mueedo C-7 for Producing The Milk-clotting Enzyme
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 449~453
This study was attempted to obtain the efficient milk-clotting enzyme from microorganisms as a rennet subtitute. Fungi which showed the formation ability of the milk-clotting enzyme were selected out from samples of soil hay and wastes etc. Among these isolated fungi, strain no. C-7 which had presented higher value in the ratio of milk-clotting activity to proteolytic activity was selected. The hyphae of this strain was white to gray and no septa. A single sporangiophore which stand erectly above growing hyphae was monomucor type without branching. A globose sporangium was developed at the tip of each sporangiophore. The suitable temperature and pH for the growth of no. C-7 was 20-
and pH 3.0-8.0 respectively. These morphological and physiological characteristics implied that strain no. C-7 was Mueor mucedo.
Transformation of Antagonistic Pseudomonas stutzeri YPL-1 against Root Rotting Fungi Fusarium solani by Plasmid DNA
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 454~459
For the genetic multipurpose of antagonistic abilities of Pseudomom etutzeri YPL-1 aganist Fusarium solani causing root rot of many important crops by genetic engineering, optimal conditions for transformation of P-stutzeri YPL-1 by pKT230 were investigated. Maxium frequency of the transformation was achieved when cells were harvested at early exponential growth phase. The highest transformation efficiency was obtained when the competent cells were exposed to chilled transformation buffer containing 20 mM RbCI, 100 mM
and added l
/ml of plasmid DNA. The pH optimum for transformation was 6.5. When the bacterial cells that were incubated during 60 minutes for the competence were brought in contact with plasmid DNA, the transformations were obtained in the best frequency. It was formed that transformation frequency was 2 ~
under the optimal conditions.
Protoplast Fusion of Cellulolytic Aspergillus wentii and Aspergillus niduk
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 460~465
Regeneration of protoplast was effective by preincubating spore suspension containing 30
/ml of 2-DG for 4 hours, and CBE medium containing casamino acid, bovine serum albumin, ergosterol and myoinositol was found to be more efficient than any other regeneration medium tested in this experiment. The regeneration frequency was about 30%. Optimal conditions for conidial protoplast fusion were obtained by treatment of protoplasts with 10 mM
and 30% polyethylene glycol 4000 (pH 7.5) as fusogenic agent at
for 10 minutes. The fusion frequency was
. The higher productivity of enzyme of fusant FWN-56 was achived: 2.3-fold for CMCase, 1.5-fold for avicelase, 1.8-fold for
-glucosidase and 2.5-fold for xylanase compared to that obtained in two parental strains. The genetic stability of fusant after maintenance on minimal medium for more than 4 weeks was high because segregant rate was below 1%. The conidial DNA content of fusant was 1.4-1.6 times higher than that of the parental strains, The nucleus size of fusants were also higher than that of each parental strains
Mutagenicity of Reaction Products of Aflatoxin
and Ascorbic Acid
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 466~470
) was reacted with ascorbic acid (AA) alone, with AA plue cysteine and with AA plus cupric ion for 5 days (at
and pH 5), and the mutagenicity of the reaction products was tested with Salmonella typhimurium TA 100. About 10% of AFBl induced mutagenesis was reduced when
reacted with AA. This decreasing effect was more severe when
reacted with AA plus cysteine. The mutagenicity of
when reacted with AA plus cupric ion was almost completely inhibited, however, eupric ion itself was shown to enhance the mutagenicity of
may be degraded in the presence of AA under the given reaction condition and the reaction products was observed to have nonmutagenic effects on the bacterial mutagenecity trials.
Characteristics of Adenylate Kinase from Extreme Thermophile Thermus caldophilus GK-24
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 471~475
A thermostable adenylate kinase isolated from the sonic extracts of Thermus caldophilus cells revealed higher substrate-specificity to the nucleoside monophosphate than to the nucleoside triphosphate. A
-di(adenosine-5') pentaphosphate was acted as a competitive inhibitor to the various substrates. Various divalent cations were activated the enzyme activity following orders:
-. The enzyme activity was not affected by the sulfurhydryl reagent, p-chloromeric uribenzoic acid and activated by addition of the sodium chloride or phosphoenol pyruvate to the reaction mixture.
Production of Hydantoinase from Streptomyces sp.
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 476~483
In order to investigate hydantoinase-producing strain of the genus Streptomyces, 523 strains of Streptomycee sp. isolated from soils were cultivated in various media and conversion activity of the enzyme was measured to DL-5-phenylhydantoin. A number of strains producing hydantoinase were detected and among them, the strain of Streptomyces sp. Y-183 was selected as a most powerful strain to producing the enzyme. The optimal culture conditions for the production of hydantoinase of the strain were studied, and it was found that almost all hydantoinase activity was produced in the cell fraction. The maximum activity of the enzyme, 17.8 unitstg of dried cells weight, was obtained when the strain was cultured at
for 72 hr in a medium containing 1.0% of glycerol. 0.5% of yeast extract. 0.5% of soytone, 0.5% of beef extract, 0.6% of KCI, 0.002% of
, and 0.4% of uracil as an inducer, and the pH of culture broth was adjusted ranging from 7.0 to 7.5.
Partial Purification and Properties of Non-specific
-fructofuranosidase Produced by Bacillus subtilis
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 484~489
An intracellular inulase ( fJ-fructofuranoside fructohydrolase, EC 18.104.22.168) from Bacillus subtilis has been partially purified and its mode of action and general properties were studied. The enzyme had an apparent molecular weight of 49,000 as estimated by gel filtration and its pI point was 5.2. Substrate concentration studies showed an apparent Km of 10 mM for sucrose and of 18 mM for raffinose. The enzyme was an acid-labile protein with a pH optimum of 6.6. The optimum temperature was 50
C. The enzyme acts on straight chain oligo- and poly-fructosides of the inulin series via a exo-wise cleavage mechanism, as well as on sucrose.
Purification and Characterization of Extracellular Inulinase from Bacillus sp.
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 490~495
The extracellular inulinase from Bacillus spp. was purified to a single protein through a sequence of operations including ammonium sulfate fractionation, heat treatment, DEAE Sepharose C1-6B ion exchange chromatography, Sephadex 6-100 and Sephadex 6-150 gel filtration. The purified enzyme was confirmed to be a
-D-fructofuranosidase(EC 22.214.171.124) which was much more active on sucrose than on inulin(I/S = 0.2). The maximal inulinase activity was observed at pH 6.0 and at the temperature of
. The mo1ecular weight of the enzyme was about 56, 000. Tryptophan and histidine residues of the enzyme molecule were found to be essential for its catalytic activity.
Properties of Pepsin Inhibitor Produced by Actinomycetes sp. GF 155-2
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 496~500
When pepsin was used at a concentration of 8 mglml for hydrolysis of 0.02% casein, inhibitory activity of this inhibitor was proportional to a inhibitor concentration of 20
/ml, and fifty percent inhibition (
) was observed to be 15
/ml. The inhibitor was pH-stable at pH range of 5-9 at
for 10 minutes and thermo-stable at pH 7.0 at
to give 100% activity for 20 minutes. The formation of pepsin-inhibitor complex was confirmed by sephadex 6-25 gel filtration and type of inhibition was determined as non-competitive inhibition by Lineweaver-Burk plot. The inhibitor strongly inhibited acid proteases such as pepsin and renin, and it was soluble in methanol very well. On TLC analysis of silicagel 60 using various sohent systems, the inhibitor gave a single spot at Rf range 0.4-0.6. From the result of IR spectrum and color reaction (Rydon-Smith, Biuret), this inhibitor was considered as peptide substance. Melting point and elemental contents were 220-
, and C 50.61%-H 8.02%-N 9.34% (found), respectively.
Characterization of Thiol Protease Inhibitor Isolated from Streptornyces sp. KISl3
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 501~505
Streptomyces sp. KISl3 isolated from soil was found to produce low molecular weight thiol protease inhibitors. The protease inhibitor production was closely linked to the cell growth and regulated by growth condition. The inhibitor was purified from the culture broth through butanol extraction, silicagel 60 column chromatography, Sephadex LH-20 gel filtration and preparative HPLC. The inhibitor showed specific inhibitory activity to thiol protease such as papain, picin and bromelain. The mode of inhibition against papain to Hammersten casein as a substrate was non-competitive.
Takju Brewing of Uncooked Rice Starch Using Rhizopus Koji
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 506~510
The Takju brewing of raw rice starch was carried out by the simultaneous saccharification- fermentation using Rhizopus sp. koji and yeast, and compared with the Takju mash brewed by the conventional method. Rhitopus koji was prepared with uncooked rice for Takju brewing without cooking of rice starch. Alcohol concentration of Takju mash brewed with uncooked rice was slightly higher of 1.8% than that with cooked one. Amino acid contents was almost double and fuse1 oil contents was lower in uncooked brewing. The Takju mash prepared after fermentation without cooking of rice had a characteristic odor of raw material and a good quality of taste.
Screening and Characterization of the High-Alcohol Producing Saccharornyces cerevisiae Dl
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 511~516
A high-alcohol producing yeast strain had sugar and alcohol tolerance was isolated from soil and identified as Saccharomyces cerevisiae Dl according to the Lodder's yeast taxonomic studies. On investigation of the characteristics of the strain, it could grow in 60% glucose, within 15% ethanol and in the YPD medium containing 2.0 mM copper. It had 39.1% the inhibition rate of fermentation efficiency and 8% viability after 2 days in the YPD medium containing 15% ethanol. Its optimum initial pH, growth temperature, initial glucose concentration for the production of alcohol showed pH 4.5,
, and 30%, respectively. Saa:hwomyce8 mvisiae Dl produced 14.0% (wlv) alcohol when incubated at
, with orbital shaking 150 rpm for 72 h in a medium (pH 4.5) containing 30% (wfv) glucose.
Protein Separation with CTAB/Hexanol/Isooctane Reverse Micellar System
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 517~524
The solubilization and desolubilization of proteins in CTAB/hexanol/isooctane reverse micellar system were investigated for the selective separation of proteins. Several proteins were used, including bovine serum albumin (BSA), pepsin, trysin and ribonuclease-a. Most proteins could be solubilized into reverse micelles in the pH range above the isoelectric point of each protein, where the net charge of protein was opposite to that of surfactant. However BSA was solubilized above pH 10, which is serveral pH units above the pI 4.9. The kinds of anions in aqueous phase influenced on protein solubilization while no significant trend was observed with different cations, Protein solubilization decreased with increase of the ion size in the order of F -, C1-, Br- and I -. The size of CTAB micelles did not change significantly with increasing ionic strength, but the solubilization decreased. Protein desolubilization showedropposite behaviors to the solubilization. Several model mixtures such as pepsin/ trypsin, pepsin/ribonuclease-a and BSAlribonucleaee-a were successfully separated from each other without changing enzymatic activities.
Bioreactor Cultures of Lithospermum erythrorhizon for Shikonin Production with In Situ Extraction
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 525~529
Plant cell cultures of Lithospermum erythrorhizon were performed in stirred tank and packed-bed reactors with in situ extraction by n-hexadecane. The specific shikonin production and volumetric shikonin productivity of stirred tank reactor reached 1.5 mg shikoninlg cell and 400
shikonin/(L.day), respectively. In packed-bed reactor with calcium alginate-immobilized cells specific shikonin production and volumetric productivity reached 2.0 mg shikoninlg cell and 2857
shikonin/(L.day), which were 1.3 and 7.1 times higher than those of stirred tank reactor, respectively. The higher shikonin production and productivity of packed-bed reactor seemed to be due to high cell loading capacity of calcium alginate immobilized cells in packed-bed reactor and improved cell-cell contact.
Treatment of Thermoactinomyces sp. to Application of Poultry Feces
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 530~534
A strain of actinomycetes, Thermoactinomyces sp. CH-53, was isolated from manure and composted livestock feces. Actinornycetes-feed additive was prepared with the solid wheat bran medium of Thermoactinomyces sp. CH-53 that grew vigorously on unsterilized poultry feces at
. pH 6.5- 9.5 and moisture content of 55-65% and added at a rate of 1% (wtlwt) to the commercially assorted feed to be fed poultry. The excreted feces contained
. Thermoactinomyces sp. CH-53 cells per gram. Poultry feces malodour was got rid of during treatment. The effect on plant growth was evaluated on the basis of the amount of nitrogen as fertilizer under a loading of 0.2g N1600g soillpot. A11 samples were showed a promotion effect for plant growth. The treated poultry feces added from O.lg to 0.4g total nitrogen per 600g soil in a pat increased the growth of Brassica rapa var. perui-ridis.
Biosyntheses of Nucleic Acids and Proteins of Bacillus sphaericus ts-Dl290 Lethal Mutant
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 535~540
Bacitlus sphaericus ts-Dl290 was characterized comparatively with the wild type strain 1593 by themeasurements of the biosynthesis of total DNA, RNA and protein on the temperature-shift culturesat permissive temperature of
and at nonpermissive temperature of
. The growth patterns of the wild type strain and ts-Dl290 were similar at
, but at 4Z C the mutant almost did not grow (temperature-sensitivity). When the growth temperatures of both stains were shifted-up from
after a 4 hour culture, their growths were normal, but when shifted-down from
after a 4 h culture, the mutant did not grow. When shifted up from
after a 4 hculture, the DNA syntheses of the two strains were at a normal rate for 1 h, but after 1 h the biosynthesesdecreased. The rate of DNA synthesis of the wild type strain at the nonpermissive temperature was about 93%, and that of the mutant was about 50% of the ratio of the wild type strain, and the RNA synthesis of the wild type strain was maintained for 3 h, and that of the mutant for 2 h. Thereafter the RNA synthesis decreased, and the synthesis of proteins in the both strains were similarlykept high for 8 h. The reversibility of the DNA synthesis of the mutant at
was lessened whenthe culture times were increased.re times were increased.
Electrophoretic Analysis of Total Proteins in Bacillus sphaericus ts-Dl290
Microbiology and Biotechnology Letters, volume 18, issue 5, 1990, Pages 541~546
Bacillus sphaericus ts-Dl290 was characterized by SDS-PAGE produced by the mutant at
. The total amount of proteins produced by the mutant at
decreased to one-fifth of those at
; however, when the culture was shifted down from
after 4 to
, the total amount of protein decreased to one-third and the 221 kd protein did not appear, but the 155 kd appeared remarkably. When the mutant and the wild type strain were cultured in the media containing 80
per ml of chloramphenicol at
, the wild type strain synthesized half amounts of the total proteins than those at
, and the mutant produced one-tenth of the total protein amounts. When the both strains were cultured in the media containing chloramphenicol, the 155 kd protein was produced was produced in lesser amounts than those without chloramphenicol. The 150 kd protein showed lethal activity to Culex pipiens 3rd instar larvae.