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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 19, Issue 6 - Dec 1991
Volume 19, Issue 5 - Oct 1991
Volume 19, Issue 4 - Aug 1991
Volume 19, Issue 3 - Jun 1991
Volume 19, Issue 2 - Apr 1991
Volume 19, Issue 1 - Feb 1991
Selecting the target year
Isolation and Identification of the Fungi Producing a Soybean Milk Clotting Enzyme
Lee, Chul-Woo ; Kang, Chang-Hoon ; Ha, Duk-Mo ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 109~115
Twenty-five fungal strains producing an extracellular soybean milk clotting enzyme were isolated from 146 soil samples, and identified as 11 species belonging to six genera of Aspergillus oryzae (5 strains), Aspergillus flavus (2 strains), Aspergillus parasiticus (1 strain), Aspergillus tamarii (2 strains), Aspergillus niger (4 strains), Aspergillus fumigatus (2 strains), Mucor hiemalis (2 strains), Wallemia sebi (4 strains), Scopulariopsis condida (1 strain), Fusarium redolens(1 strain) and Verticillum lecanii (1 strain). Among them, Aspergillus oryzae 020 and Aspergillus tamarii 287 showed relatively high soybean milk clotting activity. The coagulabilities of the enzyme from representative strains of those species decreased as the pH of soybean milk increased from 6.0 to 7.0 The optimum temperature for soybean milk clotting enzymes of those strains were 65
Isolation of Neutral Protease Hyperproducing Bacillus sp. KN103N and Some Properties of the Enzyme
Kim, Hong-Rip ; O, Pyong-Su ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 116~121
A bacterial strain KN, which highly produced a protease, was isolated from several soil samples and identified to to belong to the genus Bacillus. We selected mutant strain Bacillus sp. KN103N, which was hyperproducer of protease and was resistant to D-cyclowerine, from the strain KN by several steps of mutagenesis. Neutral protease productivity of mutant strain KN103N was about 55 times as much as that of the original strain KN. The optimum pH and temperature for the enzyme activity were 7.0 and 50
, respectively and the enzyme was relatively stable at pH6.0~8.0 and below 40
. The enzyme was inactivated by EDTA, but not by DFP. These results indicate that the enzyme from Bacillus sp. KN103N was a neutral (metallo-) protease.
Isolation of Thermostable
-Amylase Hyperproducing Bacillus sp. No. 32H417 and Some Properties of the Enzyme
Kim, Moo-Sung ; O, Pyong-Su ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 122~127
A bacterial strain NO. 32 which produced thermostable
-amylase was isolated from soil and identified to genus of Bacillus. To enhance
-amylase productivity, a successive mutation of Bacillus sp. No. 32 was attempted with treatment of N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The resulting mutant, Bacillus sp. No. 32H417, which is risistant to refampicin and deficient in spore formation, produced about 90-fold high level of
-amylase when compared with parental strain. The properties of the enzyme for thermostability were investigated. The optimal temperature and pH for enzyme reaction were 95
and pH6.5, respectively, in the presence of 0.3mM
as an effective stabilizer.
Transport and Utilization of Lactose by Alkalophilic Bacillus sp.
Yoon, Sung-Sik ; Kim, Chang-Min ; Yang, Ryung ; Yu, Ju-Hyun ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 128~134
To study the reduced growth and synthesis, proeviously reported, of
-galactosidase of alkalophilic Bacillus sp. YS-309 at the higher lactose concentration of 0.5% (w/v) in the medium, lactose transport and utilization were examined. The results showed that lactose transport was influenced by the addition of four kinds of antibiotics, and tetracycline stimulated most but not valinomycin. PEP-potentials of the cells grown on lactose was estimated lower than the cells on glucose and on galactose. Thus, the transport of lactose was independent of intracellular PEP and phosphorylation reactions, and was thought to be uptaked directly or oxidized in part in the transport process. In the other hand, once lactose was uptaked into the cells, it was hydrolyzed by
-glactosidase to glucose and galactose. The former was metabolized fast but the latter was accumulated. Galactose and lactose were not utilized until glucose was mostly depleted in the medium. The
-galactosidase synthesis decreased in the presence of glucose over 0.2% and galactose over 0.05 to 0.1%, respectively. In conclusion, it was considered for glucose as a repressor and galactose as a inducer for
-galactosidase synthesis even though the mechanisms were not elucidated. Catabolite repression of glucose on the enzyme synthesis was not relieved by the addition of exogeneous cAMP.
Gas Chromatographic Profiling for the Screening of Candida tropicalis Mutant Producing Tridecanedioic Acid
Kim, Jung-Han ; Lee, Sang-Jun ; Park, Hyoung-Kook ; Kim, Kyoung-Rae ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 135~139
Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30
, 250rpm, 48hour of culture and
as nitrogen source.
Improvement of Aspergillus niger 55, a Raw Corn Meal Saccharifying Enzyme Hyperproducer, through Mutation and Selective Screening Techniques
Oh, Sung-Hoon ; O, Pyong-Su ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 140~146
Mutation experiments were performed to select the mutant of Aspergillus niger 55, which had lost almost all the ability to produce transglucosidases but retained that of high productivity of raw meal saccharifying enzyme, by means of successive induction with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), ultraviolet(UV) light, and
-rays. Also, we used the mutant enrichment techniques, such as liquid culture-filtration procedure and differential heat sensitivity of conidia, in order to increase the possibility of obtaining a mutant. The glucoamylase productivity of mutant PFST-38 was 11 times higher than that of the parent strain. The mutant PFST-38 was morphologically identical to the parent strain, except for the size of conidia, the tendency to form conidia and the lenght of conidiophore. Asp. niger mutant PFST-38 apeared to be useful for the submerged production of the raw corn meal saccharifying enzyme.
Effect of the Phage
FSV on the Growth of Lactobacillus casei
Kim, Gyung-Tae ; Lee, Jeong-Jun ; Suh, In-Young ; Na, Seog-Hwan ; Baek, Young-Jin ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 147~152
In order to study effect of the phage
FSV on the growth of lactic acid bacteria, Lactobacillus casei YIT 9018(wild type strain with prophage) or L. casei HYM 1213 (prophage cured strain) was infected with various concentrations of phage
FSV (called Lac S21) or wild type virulent phage (called Lac J-1). When L. casei YIT 9018 was infected with Lac S21 under the concentration of
pfu/ml, the growth and lactic acid production of the strain were normal and the number of phages decreased. But L. casei HYM 1213 was susceptible to Lac S21. Regardless of the concentration of the phage infection, the number of phages increased rapidly into
pfu/ml at 2 day cultures and was maintained
pfu/ml phage until 6 day cultures. The lactic acid production of L. casei HYM 1213 infected with Lac S21 was abnormal. Therefore, phage
FSV had an evil effect on growth of L. casei HYM 1213, but not L. casei YIT 9018. On the other hand Lac J-1 caused abnormal fermentation to either L. casei YIT 9018 or L. casei HYM 1213.
Requirement of NADH dehydrogenase from an Extreme halophile, Halobacterium sp. EH10 Isolated from a Saltern in Korea
Bae, Moo ; Lee, Jeong-Im ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 153~157
Intracellular enzymes of an extreme halophilic bacterium, Halobacterium sp. HE10, isolated from a saltern in Korea was investigated. The membrane-bound enzyme, NADH dehydrogenase, involved in electron transport system was stimulated by the addition of 2.0 M NaCl. The respiratory enzyme activities such as NADH oxidase and NADH dehydrogenase was decreased on removal of
ion and restored when replaced with cations like
ions. Furthermore, their activities were affected by the anions such like carbonate, acetate, sulfate, chloride and nitrate at the presence of
ion. Lactate dehydrogenase activity was highest at the asturated solution of NaCl and isocitrate dehydrogenase activity was a maximum level at 1.0 M NaCl. These results suggested that the enzyme activites of the respiratory chain in Halobacterium sp. EH10 was stimulated by the presence of
Purification and Properties of Aspergillus ficuum Endoinulinase
Han, Sang-Bae ; Ryu, Hyang-Suk ; Rho, Min-Whan ; Lee, Tae-Kyoo ; Sohn, Hee-Suk ; Woo, Soon-Ja ; Uhm, Tai-Boong ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 158~162
Endoinulinase was purified from a commercial inulin preparation produced by Aspergillus ficuum using ion exchange chromatography on CM-Sephadex C-50 and DEAE-Sepharose 6B, HPLC gel filtration on a Protein Pak 125 Colum and HPLC ion exchange chromatography on a TSK DEAE-5pw Column. The endoinulinase had a molecular weight of 72,000
1,000 and was glycoprotein with 23 to 25% w/w sugar content. The enzyme was much more active on inulin with random cleavage mode than on sucrose and on palatinose: The ration of activity on inulin and sucrose (I/S ratio) was 10~14.
Production of Cyclodextrin from Raw Starch in the Agitated Bead Reaction System and its Reaction Mechanism
Han, Il-Keun ; Lee, Yong-Hyun ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 163~170
Production of cyclodextrin (CD) directly from raw corn starch without liquefaction using cyclodextrin glucanotransferase (CGTase) was carried out in an agitated bead reaction system. Similar CD yield and production rate comparable with those of conventional method using liquefied starch were obtained. Especially high purity-CD in the reaction mixture without accumulation of malto-oligosaccharides was obtained. The maximum 54g/l of CD was obtained at raw starch concentration of 200g/l. CD yield was inversely proportional to raw starch concentration, and conversion yield was 0.48 at substrate concentration of 100g/l. The optimal amount of enzyme (CGTase unit/g raw starch) was found to be around 6.0. Granular structure of raw starch degraded by CGTase was observed by SEM in order to investigate the enhancing mechanism, along with those of acid or alkali pretreated raw starch, amylose, and amylopectin. Kinetic constants of CGTase on raw starch in an agitated bead reaction system were evaluated, and CGTase was competitively inhibited by CD.
Continuous Production of Cyclodextrin in Two-Stage Immobilized Enzyme Reactor Coupled with Ultrafiltration Recycle System
Lee, Yong-Hyun ; Lee, Sang-Ho ; Han, Il-Keun ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 171~178
The two-stage enzyme reactor, packed with cyclodextrin glucanotransferase (CGTase) immobilized on Amberite IRA 900, coupled with ultrafiltration membrane was investigated for continuous production of cyclodextrin (CD). 5% (w/v) of soluble starch was partially cyclized, in the 0.1 l first-stage immobilized enzyme reactor, up to CD conversion yield of 10% (w/w) at retention time of 0.56hr and 1.5 units of immobilized CGTase/1g of carrier. In the second stage main immobilized enzyme reactor capacity of 1.5 l, the maximum CD conversion yield of 39% (w/v) was achieved at retention time of 2.8hr and 0.47 unit of CGTase/1 g of carrier. Unreacted residual dextrin was fractionated with ultrafiltration membrane, and then, recycled into the second-stage main bioreactor to increase the CD conversion yield. The most suitable membrane size and the volume concentration ratio (concentrate: filterate) for recycling of unreacted residual dextrin were found to be 5K dalton and 4:6, respectively. CD conversion yield was increased about 3~4% upon co-immobilization of pulluanase along with CGTase. Spent Amberite IRA 900 can be reutilized consecutively more than 3 times for immobilization of CGTase after regeneration.
Optimum Culture Conditions for Hydrogen Production of Rhodopseudomonas sphaeroides
Kim, Jihn-Sang ; Hong, Yong-Ki ; Sin, Il-Sik ; Cho, Hak-Rae ; Chang, Dong-Suk ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 179~185
We examined optimum culture conditions of Rhodopseudomonas sphaeroides B5 for effective utilization of substrate and sunlight for hydrogen production. The optimum concentration range of DL-lactate as electron donor for hydrogen production by resting cells was from 5 to 50mM, and optimun CN ratio (lactate/glutamat) for maintenence of hydrogen production activity by growing cultures was from 5 to 6. Hydrogen production by the cultures of low cell density (0.36mg/ml dry cells) was saturated with 10 Klux light intensity. Under constant illumination of 50Klux which was set up as the average medium value of annual variation of sunlight intensity, hydrogen production with various cell densities in the culture resulted in highest production rate (132
l/hr/mg dry cells) up to 0.64mg/ml dry cells. However, the amount of total hydrogen production was saturated with cell density of 2.1mg/ml dry cells. In addition to these, the optimum inner thickness pervious to light of the culture vessel for hydrogen production which was measured under sunlight was 5 cm.
Kinetics for the Growth of Alcaligenes eutrophus and the Biosynthesis of Poly-
Lee, Yong-Woo ; Yoo, Young-Je ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 186~192
It is very important to have a good kinetic model which considers the effects of both ammonium and glucose for the control and optimization of the poly-
-hydroxybutyrate (PHB) fermentation. A kinetic model for the growth of Alcaligenes eutrophus and the biosynthesis of PHB under both ammonium and glucose limitation was proposed. Growth rate of residual biomass was expressed as a function of concentrations of residual biomass, glucose and ammonium having glucose inhibition. PHB production rate was expressed as a function of concentrations of residual biomass, glucose, ammonium and PHB content having ammonium and product inhibitions. Novel approaches were made to estimate the parameters in the model equations which considered two limiting substrates. Model parameters were evaluated by graphical and simplex methods. The proposed kinetic model fitted the data very well.
Effects of pH on the Elaboration of Pullulan and the Morphology of Aureobasidium pullulans
Shin, Yong-Chul ; Byun, Si-Myung ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 193~199
The effects of pH on the cell growth, the elaboration of pullulan, and the morphology of Aureobasidium pullulans IFO 4464 were examined. A. pulluans grew in yeast-like form at constant pH7.5 and in mycelial form at constant pH2.5. At the both pH conditions, the elaboration of pullulan was very low, about 6.0~6.5g/l. The mixture of yeast-like form and mycelial form of cells was found at the constant pH4.5, at which condition, the elaboration of pullulan was high, about 24.5g/l. The pH shift experimemts showed that the specific production rates of pullulan were 0.048(
)for the mycelial form and 0.058(
)for the yeast like form, which indicated that the yeast-like form has the similar, only slightly higher, biosynthetic activity of pullulan to the mycelial form at pH4.5 and the pH of culture broth is more important factor for the elaboration of pullulan than the morphology of A. pullulans.
Enrichment of Lactic Acid Bacteria in Salted Fish, Chromis notatus
Ko, Young-Hwan ; Kim, Chang-Yong ; Kang, Dong-Sub ; Ha, Jin-Hwan ; Kim, Soo-Hyun ; Kang, Young-Joo ; Song, Dae-Jin ;
Microbiology and Biotechnology Letters, volume 19, issue 2, 1991, Pages 200~207
Jariieot is a local food prepared by fermentation of salted fish, Chromis notatus. Since its NaC' content is around 20% like other fermented seafoods, reduction NaCl concentration is desirable to minimize the risk of health hazard. Addition of KCl and enrichment of lactic acid fermentation were attempted to solve the problems resulting from low salt concentration. NaCl and KCl were added to a fish, Chromis notatus simultaneously at concentrations of 10 to 4% and 5 to 2%, respectively. Lactic acid bacteria and glucose at final concentration of 2% were also mixed with the above-salt treated fish to prepare jarijeot. The jarijeot was examined periodically for chemical changes during aging and compared with reference jarijeot containing only 20% of NaCl to find out an appropriate method for quality improvement. The content of ATP and its related compounds was not affected by the concentration of NaCl or the presence of lactic acid bacteria. Nearly no difference in contents of free amino nitrogen, trimethylamine oxide, trimethylamine and volatile basic nitrogen was observed between the jarijeot containing 20% of NaCl only and that containing 10% of NaCl, 5% of KCl, 2% of glucose and cells of Pediococcus halophilus. Moreover, sensory evaluation of both kinds of jarijeots revealed almost the same scores. The number of cells of P. halophilus was maintained at concentration of
cell/ml for 60days' fermentation in the above mentioned jarijeot containing 10% of NaCl. Its pH was dropped down to 4.2. Accordingly it is possible to prepare jarijeot enriched with lactic acid bacteria if KCl and glucose are added at concentration of 5% and 2%, respectively, in addition to NaCl at a final concentration of 10%.