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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 19, Issue 6 - Dec 1991
Volume 19, Issue 5 - Oct 1991
Volume 19, Issue 4 - Aug 1991
Volume 19, Issue 3 - Jun 1991
Volume 19, Issue 2 - Apr 1991
Volume 19, Issue 1 - Feb 1991
Selecting the target year
Isolation and Identification of Alkalophilic Microorganism and its Mutant Growing at Neutral pH
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 543~547
An alkalophilic microorganism, SH-8, was isolated from soil samples. It was a Grampositive, catalase-positive, spore-forming and motile rod which was capable of growth in aerobic condition at the initial pH 9.0 or above up to 11.0 and between 15 and
The characteristics of this strain resembled those of the Bacillus group of bacteria. The mutant, Bacillus sp. SH- 8M, was selected from Bacillus sp. SH-8 by U.V. mutagenesis and was able to grow at pH 6.9 up to 11.0. These two strains will be suitable for the comparative study on growth and enzyme production at various pH conditions.
Effect of Dual Inoculation with Glomus mosseae and Bradyrhizobium sp. R938 on the Nitrogenase Activity of Arachis hypogeae L.
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 548~552
The effect of dual inoculation with vesicular-arbuscular (VA) mycorrhizal fungi, Glomus mosseae, and Bradyrhizobium sp. R938 on the nitrogenase activity of peanut plant (Arachis hypogeae L. cv. Youngho) was studied in pot experiment. As a result of the dual inoculation with Glomus mosseae and Bradyrhizobium on peanut plant, nitrogenase activity was increased by 1.5 times compared with single inoculation with Bradyrhizobium sp. R938. After 69 days growth of peanut plant, the size of nodules was also increased due to the mycorrhizal infection. The mean fresh weight per nodule was 6.7 mg in treatment with the dual inoculation, which is the 1.5 times increase compared with single inoculation with Bradyrhizobium sp. R938. These results suggested that there might be a hormonal effect of VA mycorrhizal fungi in addition to the effect of increase in nitrogen fixing ability through the enhancement of nutrient absorption.
Killing Activity and Molecular Properties of Bacteriophage Sigma FA1 of Bacillus circulans
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 553~560
In the previous paper (10). a new temperate phage, Sigma FA1 had been isolated from B. circulans. Sigma FA1 had an icosahedral head with a diameter of about 70 nm, and a tail about 15 nm long, and beared a circularly permuted, linear duplex DNA. Signla FA1 killed sensitive cells by a single-hit process. Phage DNA injected into the cell immediately after infection was degraded slowly. Our results indicate that the killing action of Sigma FA1 is different from the phenomenon of abortive infection and suggest that the killing might be caused by a proteinaceous component of Sigma FA1.
Identification of the Actinomycetes Strain No. 497, Isolated from Soil, Producing Actinomycin Antibiotic MT-497
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 561~567
Identification of the Actinomycetes isolate strain No. 497 producing an actinomycin antibiotic MT-497 was performed by ISP and chemotaxonomic methods. The strain Nu. 497 formed various shapes of sclerotia and smooth surface spore. Menaquinone MK-9 (
) and iso-, anteiso-branched
fatty acids were detected from whole cell extract. The wall chemotype of stram No. 497 was decided as wall chemotype I from the analysis of DAP isomer, peptidoglycan type and sugar pattern. From these morphological, chemotaxonomic characteristics and analysis of various physiological characteristics. the strain No. 497 was identified as Streptomyces nigrifaciens.
Construction of an Expression Vector System with the GAP Promoter in Saccharomyces cerevisiae
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 568~574
The cloned glyceraldehyde-3-phosphate dehydrogenase (GAP) gene of Saccharomyces cereviszae (Holland et al., 1983) has been characterized. Based on the communication, we have also cloned 2.1 kb CAP DNA fragment and modified this fragment as a portable promoter. Two yeast expression vectors, one is YCp type vector being maintained at low copy number (1 or 2) and the other is YEp type vector at high copy number, have been constructed with the GAP promoter and the PH05' gene as a reporter. Our plasrnids were introduc,ed into S. cerevisiae HY-1, which has been improved. The
transformants expressed APase activity efficiently and showed high level of PH05' transcripts.
Cloning of Genes for the Biosynthesis of Glutathione from E. coIi K-12
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 575~582
To increase the production of glutathione by the expression of recombinant gsh plasmids, two genes responsible for the biosynthesis of glutathione were isolated and cloned. To clone a gshI gene, the GS903 mutant strain, which is deficient in
-glutamylcysteine synthetase activity, has been raised. A gshI gene was cloned using pBR322 plasmid as a 3.6 Kb PstI DNA fragment isolated from E. coli K-12 chromosomal DNA. Also a gshIl gene was cloned using pUC13 plasmid as a 2.2 Kb PstI-BamHI DNA fragment. To study the effects of plasmid copy number and passenger DNA size on the expression levels of the gsh genes, various recombinant plasmids containing different sets of genes were constructed. The expression levels of the gsh genes were increased approximately twice higher in pUC series plasmids than that in pBR322 plasmid. But the sizes of the passenger DNA containing the gsh genes in the vector plasmid did not affect on the expression levels of the gsh genes.
Production of L-Threonine by Auxotrophs and Analogue Resistant Mutants of Escherichia coli
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 583~587
A threonine overproducer, E. coli TF427, which is resistant to threonine analogue, a-amino-(3-hydroxyvaleric acid (AHV), and requires both methionine and isoleucine was developed by the mutations of E, coli W3110 using N-methyl-Nf-nitro-N-nitrosoguanidine (NTG) and UV. The E. coli TF427 produced 46.5 gll of threonine in a 5-L jar fermentor after 44 hr cultivation. The aspartokinase I of TF427 was not inhibited by threonine, and its synthesis was not repressed by threonine plus isoleucine.
Laboratory Scale Preparation of S-Adenosyl-L-Methionine from Yeast
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 588~591
S-adenosyl-L-methionine (SAM) is essential substrate for biological methylation reactions. The present work describes a reoptimized procedure of SAM preparation in laboratory scale by the method of yeast fermentation. The fermentation medium enriched with methionine and the culture conditions were reoptimized. The isolation steps consisted of 5 steps including extractions, precipitations, and chromatography. This improved procedure over original method provides relatively high yield of biologically active product within a 4 day-period.
Purification and Characterization of an Extracellular Xylanase of Bacillus stearothermophilus
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 592~597
An extracellular xylanase of Bacillus stearothemophilus was purified to a single protein through a sequency of operations including ammonium sulfate fractionation, DEAE Sepharose CL-6B ion exchange chromatography, Sephadex G-100 gel filtration and heat treatment. The purified enzyme had a moleular weight of 170, 000. the pH and temperature optima for the enzyme activity were pH 9.0 and
, respectively. The activity was enhanced by
, and inhibited by
. Pattern of hydrolysis demonstrated that the xylanase was an endo-splitting enzyme able to break down larchwood xylan at random giving xylobiose and xylotriose as the main end products.
Structure Determination of Antifungal KRF-001 Produced by Bacillus subtilis subsp. krictiensis
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 598~603
An antifungal mixture of six members (component A to F), KRF-001 produced by Bacillzts subtilis subsp. krictiensis was isolated from the fermentation broth. Molecular weight of component A to F was determined by FAB-MS to be 1042, 1056, 1056, 1070, 1070 and 1084 respectively. Various instrumental analyses (amino acid analysis, GC-MS,
COSY NMR) revealed that the mixture was a homologous cyclic peptide composed of each one mole of glutamine, proline, tyrosine, serine, unusual
-amino acid and three moles of asparagine. The structural differences of component A to F were found in carbon number and terminal structure of the unusual
-amino acid. After determination of the sequence and stereochemistry of those amino acids, the tentative structure of KRF-001 was determined.
Purification and Chemical Characterization of Antibiotic MT-497 Produced by Streptomyces nigrifaciens GMT-497
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 604~609
Antibiotic MT-497 was purified from the culture broth of Streptomyces nigr
and composition of amino acids, MT-497 was identified as one of the actinornycin antibiotics containing actinocin chromophore and the peptide with threonine, proline, methyl valine, sarcosine and aspartic acid.
Some Properties of Xanthine Dehydrogenase from Pseudomonas synuantha A3
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 610~613
Some of the Kinetic properties of crystallic xanthine dehydrogenase form Pseudomonas synxantha A3 were studied. The enzyme activity was strongly inhibited by adenine, 8-azaadenine, 2-methyladenine, guanine, and 8-azaguanine, but not by caffeine, and the inhibitions by adenine and guanine were observed to be of noncompetitive type. The
values for adenine and guanine were 0.037 and 0.098 mM, respectively. Michaelis constants were found to be 0.33 and 0.06 rnM for hypoxanthine and xanthine with
as the second substrate, respectively, and 0.1 rnM for
with either hypoxanthine or xanthine as the second substrate.
Effect of Some Factors on the Production of an Antifungal Compound KRF-001 from Bacillus subtilis subsp. krictiensis
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 614~618
Antifungal compound, KRF-001, was produced by Bacillus subtilis subsp. krictiensis isolated from soil. Physico-chemical factors affecting cell growth and bioactivity were examined to improve the production yield. Nutrient composition, temperature, pH and phosphate ion concentration were proved to be important factors for the production of KRF-001. Mutation was performed to select high yielding strains. First, mutation was performed with ultra-violet light, and the second mutation process was conducted by MNNG (N-Methyl-N'-nitro-N-nitrosoguanidine) resulting in three high yielding strains.
Charaterization of Nisin Production and Resistance of Lactococcus lactis ssp. lactis
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 619~623
To investigate nisin production and resistance of Lactococcus lactis ssp. tactis ML (L. lactis
, effects of medium, pH of culture broth, and cell growth on the nisin activity, and effect of nisin with or without
ion on the growth of L. lactzs were analyzed. In the bio-assay of nisin by the agar diffusion method, inhibition-zone diameter of Micrococcus Javus was propotional to the logarithm of nisin concentration ranged 0.5~20 unitlml (12.5~500 ng/mf). Nisin activity of the pasteurized culture filtrates of L. lactis MLs was high at pH 2!3 but was inactivated completely at pH over 6.0. Nisin production of the L. lactis
cultured on LTB broth increased at late logarithmic phase and reached 10.5 unitlml after 16 hr. The cell growth of L. lactis LM 0230, a plasmid free and nisin sensitive strain, was inhibited on agar medium containing 7 unitlrnl of nisin, while L. lactis
showed high survival ability at 20 unitld of nisin. When 40 mM
ion was added to Elliker broth with 8 unitlml of nisin, the growth pattern of L. lactis
was similiar to that on control medium which did not contain nisin and
ion, and this suggested that
increased the nisin resistance of the L. lactis.
Numerical Taxonomic Studies of Phenol-degrading Bacteria Isolated from Sail
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 624~630
Sixty five phenol degrading bacteria were isolated from soil and identified. Sirnility values calculated on the basis of total 46 morphological, biochemical and physiological characteristics of the isolated strains. 65 isolates were divided into 6 clusters at the 70% simility lavei, The dominant organisms were belonged to Azotobacter, Pseudomonas and Flavobactwium.
Microbial Degradation of Aromatic Compounds in Industrial Wastewater
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 631~636
The bacteria which can biodegrade aromatic compounds were screened from soil and wastewater. The isolated Pseudomonas sp. HC107 had high removal rate of COD and phenol. And also this strain grew on m-cresol, salicylate, toluene, 2, 4-D and benzene. When the strain culture (2 ml/day) was treated on continuous reactor at mixed wastewater from chemical, pharmaceutical and dye industry, the treatment rate of COD, BOD and phenol was to be about 92.5%, 95.3% and 93.5%, respectively.
Antimicrobial Activity of Lactobacillus plantarum Lp2 Isolated from Kimchi
Microbiology and Biotechnology Letters, volume 19, issue 6, 1991, Pages 637~643
The inhibitory activity of Lactobacillus plantarum Lp2 isolated from Kimchi was investigated against E. colz, Staphylococcus aureus, Str. faecalis, P. aeruginosa and psycrotrophic strain Pcl. The addition of ether extract from Lp2 culture broth completely inhibited the growth of E, coli, P. aeruginosa and Pc1, but the inhibitory action was weak against S. aureus and Str. faecalis. The inhibitory substance(s), soluble in methanol, acetone and ethyl ether, was active in the pH range of 5 and 6, but the activity was lost above pH 6. The activity was retained after heating at
for 15 min. By dialysis, the molecular weight of the inhibitory substancek) was estimated under 1000. The TLC analysis of Lp2 ether extract revealed the presence of three bands and the inhibitory activity was found in that of