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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 2, Issue 3 - Sep 1974
Volume 2, Issue 2 - Jun 1974
Volume 2, Issue 1 - Mar 1974
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Studies on the Utilization of Agricultural Wastes (Part 2) Isolation and Identification of Cellulose Utilizing Bacteria.
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 1~7
For the purpose of producing cellulosic single-cell protein from the agricultural wastes, 172 strains of cellulose-assimilating bacteria were isolated from 102 samples of rotten woods, compost soils, soils and so on by the enrichment culture technique. The isolates were examined for their ability to utilize cellulose as carbon source, and then six strains were screened by their strong cellulose assimilating ability and identified as follows: 1. Among six strains of bacteria screened, five strains were identified as species belonged to the genus Cellulomonas and the remainder to the genus of Sporocytophaga. 2. The isolated Sporocytophaga species was identified as S. ellipsosporn because it has a ellipsoidal microcyst. 3. The isolated Cellulomonas species were identical to a strain of C. fimi, C. aurcgena, C. gelida, respectively and two strains to C. flavigena. 4. The isolated C. aurogena was proved to be a new variety becauuse it has different characteristics of assimilating pentoses such as arabinose and xylose from the strain discribed in Bergey's Manual.
The Effect of Astradix-P on the Growth of Yeast for Putrefing Soy Sauce
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 9~11
This experiment was carried out to conform the antseptic effect of Astradix-P, as a yeaststatic substance on the growth of film forming yeasts, which were isolated from denatured home prepared Korean soy sauce. It resulted that Astradix-P did not give any antiseptic effect, if these yeasts were inoculated into soy sauce medium, but in the ordinary medium the yeast growth were strongly inhibited. Consquently the possiblities of the practical application of the Astradix-P into the home-preserving soy sauce was primarily remained in doubt at this moment. This result might be caused from the reason that the basic amino acids, originally existed in soy sauce, eg, arginine, histidine and lysine etc., had anticipated with the antagonistic action and thereby they made the Astradix-P inactive to these yeast, which already they have been recognized in the previous works.
Studies on the Protease procuced by Streptomyces sp.
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 13~17
A strain of Streptomyces sp. which producing a metal containing proteolytic enzyme was isolated from soil and some properties of this enzyme were investigated. The following results were obtained. 1. Optimal pH and temperature of this enzyme were pH7.0 and 37
2. This enzyme was easily inactivated with heat treatment: for example, by the treatmentat 37
for 100 minutes the activity of the enzyme was decreased to about 50 per cent of intial actively but this enzyme was stable at neutral pH. 3. The activity of this enzyme was not inhibited by
ect. but strongly inhibited by Hg
, or Cd
. 4. This enzyme was strongly inhibited by EDTA but was not inhibited by oxalic acid, citric acid, 2, 4-dinitrophenol,
-aminocaproic acid, cysteine, or thiourea.
Studies on the Population of Toxigenic Fungi in Foodstuffs (Ⅶ) Toxicological approches to the Penicillium sp. metabolites isolated from foodstuffs
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 19~27
Thirty one culture filtrates of Penicillium app. isolated from foodstuffs were submitted for toxicity by use of HeLa cell and ICR-mice. Nine strains among the 31 of Penicillia were cytotoxic to the HeLa cell cultures. Seven strains among the 31 of Penicillia were toxic to the ICR-mice and the pathological findings were the liver injury featured by parenchymal cell necrosis and degeneration. As a mass screening, cytotoxicity test using HeLa cells is feasible method to detect the mycotoxin-producing fungi.
Production of 3-Ketosteroid-delta-1-Dehydrogenase by a Two-stage Continuous Culture
D. Y. Ryu ; Lee, B. K. ; R. W. Thoma ;
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 29~35
We have studied the applicability of the principles and inherent advantages of the two-stage dontinuous uclture technique to an enzyme process for the purpose of improving and optimizing the productivity of 3-ketosteroid-delta-1-dehydrogenase. By using a two-stage continuous culture system, the growth st ageand enzyme produdtion stage are separated. In each stage an optimal set of toperaing conditions was determined, and this was tested for feasibility for the period of 10 days. During this period, at least 70％ of the maximum enzyme productivity could be maintained. The important design parameters studied are: (1) optimal specific growth rate in the first stage which corresponds to the maximal cell productivity, (2) the optimal dilution rate in the second stage which in turn determines the size of second stage fermentor and the mean residence time of cells in the second stage, (3) cell concentration in both stages, add (4) the specific enzyme productivity and enzyme productivity of the second stage. In addition, by using two-stage continuous culture system we have been able to reduce or eliminate the effect of catabolite repression due to high medium concentration and the adverse effect of the solvent used to dissolve the inducer. We have found the balance between the opposing effects of induction and repression in the second stage judging from the observation that the enzyme productivity goes through a maximum.
Studies on Cysteinedesulfhydrase produced by Bacteria.(Part 1) Isolation and Identification of Cysteinedesulfhydrase producing Bacterium.
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 37~43
1. In the course of investigation on the metabolism of cysteine by microorganisms, the authors have found that among 70 strains tested Cysteinedesulfhydrase occured most remarkably in the cells of the strain I-3-2 isolated from the soil when it was grown on a medium containing L-Cysteine. The morphological, cultural and physiological properties of this strain were investigated. From the results, the bacterium was identified as a variety of Aerobactor aerogenes. 2. The cultural conditions for the formation of Cysteinedesulfhydrase by the strain I-3-2 were also investigated and the results were as follows: 1). The optium pH of the culture medium was 7.0-7.5. 2). As a carbon source, glycerol was most effective when it was added to the basal medium at 0.1 ％ concentration. 3). By addition of calcium chloride at 0.2％ concentration, the formation of the enzyme remarkably increased. 4). Maximal formation of the enzyme was observed at the end of logarithmic phase of cell growth, thereafter the enzyme activity was diminished rapidly.
Studies on Cysteine desulfhydrase Produced by Bacteria(Part II) Enzymatic Preparation of L-Cysteine Derivatives by Cysteinedesulfhydrase from Aerobacter aerogenes.
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 45~50
1 With cysteinedesulfhydrase (E. C.188.8.131.52.) from Aerobactor aerogenes, an enzyme which catalyzes the stoichiometric conversion of L-cysteine to pyruvate, ammonia and sulfide, reversibility of the degradation of L-cysteine was investigated. It was found that the enzyme also catalized the reverse reaction of
-elimination to synthesize L-cysteine derivatives from pyruvate, ammonia and sulfides when large amounts of substrates were added to the reaction mixtures. 2. The synthetic reaction by cysteinedesulfhydrase proceeded linearly with incubation time and enzyme concentrations. The optimal pH for the synthetic reaction was 10.0. 3. The results of the isolation and identification of the products showed that the L-cysteine derivatives synthesized by this enzymatic method were identical with S-methyl-L-cysteine and S-ethyl-L-cysteine respectively.
발효법에 의한 초산으로부터의 글루타민산소오다 제조
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 51~55
Cheese 제조와 미생물응유효소 Mucor-rennin
Microbiology and Biotechnology Letters, volume 2, issue 1, 1974, Pages 57~59