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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 2, Issue 3 - Sep 1974
Volume 2, Issue 2 - Jun 1974
Volume 2, Issue 1 - Mar 1974
Selecting the target year
Optimal Operation Design of Continuous Enzymatic Reactor System
Namkoong, Shik ;
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 63~77
The optimal operation problem of enzyme reactor of double inhibitions are dealt with Maximum Principle and Steepest Ascent Method. The operation policy of the intial concentration and amount of substrate, reaction time and the method of cross feed of substrate are determined in a system of reactor of constant volume add with cross feed of substrate. The policy for the soluble enzyme and the immobilized enzyme are greatly different from each other, and the performance index, the profit per unit time, of the latter are nearly twice greater than that of the former.
Studies on the Microbial Utilization of Agricultural Wastes (Part 3) Effects of Alkali Treatments of the Wastes on the Production of Cellulosic Single-Cell Protein
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 79~82
Present experiments were designed to estimate the effects of pretreatments by various kinds of alkalis to the agricultural wastes such as cereal straws as the substrate on the production of cellulosic single-cell protein. Among the various kinds of alkalis NaOH was proved to be the most effective on improving the digestibility of cellulose by the bacteria isolated. NH
OH which is inferior to NaOH in the effectiveness of treatment might have more economic advantage in the price, and the ammonium salt resulted from the neutralization can be used as the nitrogen source by bacteria. The treatment with higher concentration than 1 normality of NH
OH didn't increase the productivility of cell mass. About five per cent of (NH
in medium resulted from the neutralization didn't have any influence in the cell mass productivility. Futhermore, the cell mass productibility was higher in the case of neutralization than alkali free washing. The digestibility of straws was increased from 7.9％ to 46.0％ by NH
OH treatment, and 6.3∼6.45g of dry cell were obtained from 40g of NH
OH treated straws. In the case of NaOH treatment, 8.6g of cell mass was obtained from 40g of substrate.
Studies on the Utilization of Alcohol Distillers' Waste. Part 1. Production of Saccharomyces cerevisiae Cells from Alcohol Distilled Waste of Wheat-flour.
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 83~88
The water pollution by waste water is one of the important issue and the short of animal feed is too, in Korea. So, this experiment is accomplished to treat alcohol distillers' waste by micro-organisms and planning to produce yeasts, which can be used as animal feed, pharmacy and condiments. 1. The raw material, alcohol distillers' waste, of this experiment consists of insoluble solids (residue) and filterate (supernatant). The residue contains 33.08％ of crude protein, 19.96％ of total sugar. and 2.06％ of ash, respectively. On the other hand the flterate through the Toyo filter paper No. 5C, contains 2.48％ of crude protein, 1.54％ of reducing sugar, and 0.43％ of ash, respectively. 2. Optimum pH of the basal medium for the growth of Saccharomyces cerevisiae YF-1 is 4.0. Optimum culture condition of this is as follows : when 0.43g of urea, 0.43g of potassium phosphate monobasic, and 0.21g of magnesium sulfate are added to the 100m1 of basal medium. Optimum temperature and optimum incubation time are 30
and 24-28 hrs. 3. Under these conditions, the maximum yield of dry yeast is 1.38％ to the medium. 4. The composition of dry yeast, produced under these conditions, is as follows: crude protein, 56.96％, lipid, 1.30％. total sugar, 6.53％, and ash 9.62％.
Studies on the Substitution of Raw Material for Soy Sauce Part IV. Use of Wheat gluten
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 89~93
The various conditions of substituting wheat gluten for the bean, one of the raw materials for soy sauce manufacture, was studied by measuring the activities of the amylase and proteolytic enzyme of koji. It was found that substitute wheat gluten for up to 60％ of bean content (30％ of the total bran and wheat content) yielded good quality of soy sauce. By using more than 30％ of wheat gluten the availability of nitrogen of raw materials was decreased. This was attributed to the low enzyme activity in koji containing more than 30％ wheat gluten.
Studies on the Production of Yeast. (Part 1) Yeast Production from the Hydrolyzate of Sweet Potato Starch Cake as a Carbon Source
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 95~101
Studies on the optimum conditions of acid hydrolysis of sweet potato starch cake and its utilization on the production of Saccharomyces cerevisiae as a carbon source were conducted and the results showed as follows; 1.The highest hydrolysis rate, 62.7 ％ of the reducing sugar based on the weight of the dry matter, was obtained when the starch cake was hydrolyzed with 1.0％ of hydrochloric acid at 2.0 kg/
for 30 minutes. 2. But the yeast grew most favorably on the hydrolyzate obtained by treating the starch cake with 0.5％ of hydrochloric acid at 2.0 kg/
for 10 minutes. Reducing sugar content of hydrolyzate was 51.4％. 3. The optimum pH of the culture medium was 7.0, Cell growth reached to the maximum at 36 hours of cultivation time. 4. According to the vitamin requirement tests, Ca-pantothenate was found to be a promoting factor for the growth of the yeast cells. 5. "Gluten acid hydrolyzate" was most effective to the cell growth when added to the medium at the concentration of 0.1％ as a nitrogen source. 6. Sacch. cerevisiae could assimilate the sugars in the hydrolyzate about 89.1％, and the yields of the yeast cells showed 23.2mg/ml of culture medium.
Studies on the Bacterial Production of L-Glutamate from Acetate Part I. Screening and Identification of L-Glutamate Producing Bacteria.
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 103~109
In the cource of the studies on L-glutamic acid production from acetic acid, 383 strains capable of assimilating acetate as sole source of carbon were isolated from 279 kinds of soil sample. Out of them, 5 strains which produced relatively larger amount of L-glutamate from acetate were selected and named Brevibacterium flavum nov. sp. D1005B, Corynebacterium glutamicum nov. sp. D1025A, Brevib. flavum nov. sp. D2209B, Coryneb. acetoacidophilum nov. sp. D2212B and Coryneb. acetoacidophilum nov. sp. D2349A respectively.
Studies on Naringinase of Mold. (Part 3) Naringin solubilizing enzyme of Aspergillus niger S-1 and removing of bitter taste from chinese citron.
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 111~117
Studies were carried out on the practical use of Naringinase and some chracteristics of Naringin solublizing enzme which might hydrolyae naringin to purunin. Obtained results were as follows. 1. Selected strain for Naringinase producing was identified to be Aspergillus niger S-1 and its naringinase was applied to chinese citron processing to remove the bitter taste. 2. Of the naringinase, naringin solubilizing enzyme was purified on a DEAE-Sephadex A-50 column and crystalized from acetone and ammonium sulfate. 3. Hydrolized naringin which has higher solubility rather than naringin or naringenin were identified by thin layer chromatography. 4. Hydrolyzed naringin and naringin were separatly determinated by ethylacetate extraction and this result was compared with sensory test.
Microbial Production of Nucleic Acid Related Substances
Ogata, K. ;
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 119~119
Microbiological Synthesis of L-Tyrosine and 3,4-Dihydroxy-Phenyl-L-Alanine
Yamada, H. ;
Microbiology and Biotechnology Letters, volume 2, issue 2, 1974, Pages 121~122