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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 21, Issue 6 - Dec 1993
Volume 21, Issue 5 - Oct 1993
Volume 21, Issue 4 - Aug 1993
Volume 21, Issue 3 - Jun 1993
Volume 21, Issue 2 - Apr 1993
Volume 21, Issue 1 - Feb 1993
Selecting the target year
Isolation, Identification and Culture Condition of Microorganism Producing Glutathione
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 1~5
The strain which produced glutathione was isolated from soil samples. The isolated strain was identified as the genus Candida through its morphological. cultural and physiological characteristics. The highest production of glutathione by Candida sp. was obtained after cultivation with shaking for 36 hours in the culture medium containing fructose 1.0%, yeast extract 4.0%. NaCI 0.04%, thiamine-HCI
/ml, and L-cysteine 0.04%. The optimal pH and temperature for the glutathione production were pH 6.0 and
, respectively. The glutathione production by Candida sp. under the optimal culture condition was
Identification and Cultural Characterization of Streptomyces Zydicus G-23 for Producing Chitinase
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 6~12
Among 294 Actinomycetes isolated from soil, a strain that had appeared to produce the highest level of chitinase selected for further studies. The selected strain was identified as a Streptomyces lydicus based on the data obtained from the morphological, biochemical and cultural experiments. The cultural conditions for the enzyme production were also examined, and the results obtained were as follows: the maximal enzyme production was attained when the cells were cultured at
for 6 days in the medium supplemented with 2% of colloidal chitin. The optimum initial pH of the medium was observed to be 8. It was also found that the most effective carbon and nitrogen sources were soluble starch and ammonium oxalate, respectively.
Enzymatic Reactions in Citric Acid Fermentation of Mandarin Orange Peel by Aspfrgillus niger
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 13~17
When mandarin orange peel was used for a substrate of citric aCid fermentation by Aspergillus niger, principal enzyme activities were investigated. Not only the activity of polygalacturonase and pectin esterase being capable of digesting pectin and crude fiber of mandarin orange peel. but also that of carboxymethyl cellulase, xylanase and amylase was high. In carbohydrate metabolism, the activity of enzymes related in HMP pathway was higher than that in EMP pathway at the orange peel medium designed hereby rather than synthetic medium. Productivity of citric acid was significantly increased when the activity of citrate synthetase was high and 5imultaneously those of aconitase and NADP-dependent dehydrogenase were low.
Screening of the Endoinulinase-producing Fungi by Using Antibody
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 18~22
An assay system by using antibody was adopted to screen the endoinulinase producingfungi due to its high specificity toward endoinulinase, To determine whether the affinity-purified rabbit serum, which were generated against the purified endoinulinase, can react only with the endoinulinase, rocket immunoelectrophoresis was performed. The results showed that the serum specifically reacts with endoinulinase but not with exoinulinase and other proteins in the culture media. Using this polyclonal antibody, a strain from 62 fungal colonies was selected and it secreted an endoinulinase in the culture media to the amount comparable to that of Aspergillus ficuum A Tee 16882 known as a high endoinulinase producer.
Purification and the Stoichiometry of Nucleoside Oxidase from Flavobacterium meningosepticum
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 23~29
A bacterial strain. producing a nucleoside oxidase was isolated from soil and identified as Flavobacterium meningosepticum by its taxonomical characteristics. The enzyme has been purified ISO-fold to electrophoretic homogeniety in an overall yield of 18% from the cell free extract of the producer. The enzyme catalyzed oxidation of only nucleosides related to both purine and pyrimidine with very high substrate specificity. The nucleoside oxidase was proved to be a noble enzyme by stoichiometry that 1 mol adenosine as a substrate was especially oxidized via adenosine 5' -aldehyde to 1 mol adenosine 5' -carboxylic acid with the formation of 2 mol
Properties of ATPase Activity of ATP-dependent Clp Protease in Escherichia coli
;Michael R. Maurizi;
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 30~35
Clp is a relatively abundant ATP-dependent protease found in E. coli. Its specific activity was proportional to the concentration of the limiting amount of Clp A and an excess amount of Clp P, and vice versa. Clp A has an intrinsic ATPase activity that is stimulated by casein, and contains a second site for binding A TP, in addition to the ATPase site. The modification of sulfhydryl groups in Clp A with reagents which have bulky groups such as N-phenylmaleimide led to nullifying both ATPase and protease activity. The same sites were modified by sulfhydryl reagents. It seems that the sulfhydryl groups of Clp A are not directly involved in catalysis. Since non-hydrolyzable analogs of ATP do not activate Clp, ATP hydrolysis may be essential for the proteolytic activity of Clp protease. Clp A and Clp P did not associate in the absence of nucleotide. The results suggest that the activity of the proteolytic component, Clp P, is regulated by the A TP-dependent cycling of Clp A between the activator form and the non-activator form.
Production of Inulin Fructotransferase (Depolymerizing) from Enterobacter sp. S45
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 36~40
A bacterial strain, producing extracellular inulin fructotransferase which converts inulin into di-D-fructofuranose dianhydride (DFA) was isolated from soil and presumed as Enterobacter sp. The DFA isolated on Bio-gel P2 column was identified as DFA III by high performance liquid chromatography and
spectroscopy. The enzyme production was induced by inulin and markedly enhanced by the addition of corn steep liquor and
for nitrogen source. Under optimum condition, the enzyme activity in the culture broth reached at maximum, 0.22 unit/ml after cultivation for 72 hour.
Identification of the Antibiotic-Producing Chstridium sp. KH-431 and Purification of the Antibiotics
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 41~46
A strain showing antibiotic activities against various bacteria and fungi was selected from approximately 2,000 microorganisms obtained from soil samples. This strain, designated as KH-431, was identified as a Clostridium sp. by its morphological, physiological and biochemical characteristics. The highest production of the antibiotics was achieved in a fermentation medium containing sorbitol, yeast extract, d-biotin and
The antibiotics were isolated from the culture broth by solvent extraction using ethyl acetate, silica gel column chromatography and recrystallization. Two kinds of antibiotics, KG-431A and KG-431B were obtained after the purification procedure, and only KG-431B was successful to recrystallize.
Characterization and Structural Determination of the Antibiotics Produced by a Clostridium sp. KH-431
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 47~53
Antibiotics KG-431A and KG-431B were isolated from the fermentation broth of the Clostridium sp. KH-431. As we have shown previously, only KG-431B was successful to recrystallize. These antibiotics showed antimicrobial activities against broad spectrum of bacteria and fungi. KG-431B also showed anticancer activity against some animal tumor cells according to the SRB method. Physico-chemical properties of KG-431B were determined using UV, IR, NMR and Mass spectra. It was identified to be 3-Indole propionic acid and the structure of the KG-431A is currently under investigation.
MT-2007, Protein Kinase C Inhibitor from Aetinomycetes Isolate No. 2007-18
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 54~58
During the screening of inhibitors against protein kinase CCPKC) and the bleb formation of K562 cell induced by phorbol ester from microbial secondary metabolites, MT-2007 was purified by solvent extraction, and chromatographic techniques from Actinomycetes isolate No. 2007-18. It showed completely suppression of bleb formation of K562 cell surface induced by phorbol 12.13dibutylate at the concentration of 503.9 11M and ICso on PKC was 31.4 11M. Its structure was postulated as lasalocid A sodium salt by physico-chemical properties and UV, IR. MS, IH-NMR.
Purification and Biological Activities of MT 1155 Inducing Morphological Change of Rous Sarcoma Virus-Transformed Normal Rat Kidney Cell
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 59~65
We isolated Actinomycetes strain GMT 1155 and purified the active compound, MT 1155, on the morphological reversion of ts/NRK cell from the isolate. MT 1155 was identified as toyocamycin having antifungal and antitumor activities from physico-chemical properties and UV, IR,
-NMR and mass spectrum. MT 1155 showed the morphologically reversional activity on ts/NRK cell and the cytotoxicity on CTLL cell at the final concentrations of 1.7 JlM and 0.2 11M, respectively and its
value on protein kinase A enzyme was 2.3
M. Also it had strong antifungal activity against several pathogenic fungi but not antibacterial activity. And it did not inhibit both protein kinase C activity and the bleb-formation of K562 cell induced by phorbol esters.
Isolation and Identification of the Crude Oil-degrading Psychrotrophic Bacterium and the Characteristics of OCT Plasmid
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 66~73
Psychrotrophic bacterial strains utilizing crude oil as their sole carbon and energy sources were isolated from Antarctic soil and sea sediments. One of the strains named AI-I showed the hightest activity for emulsification of crude oil and the best growth. This strain was identified as Acinetobacter calcoaceticus. A. calcoaceticus AI-I strain contains a plasmid (OCT plasmid) which was related to the utilization of alkane compounds. The molecular weight of this plasmid was estimated to be about 110 Md by agarose gel electrophoresis. The cured strain of A. calcoaceticus AI-I strain (OCT ) was not able to utilize normal hydrocarbon compounds (
) as carbon and energy sources. A. ca/coaceticus AI-1 was resistant to ampicillin and sensitive to streptomycin, kanamycin, chloramphenicol, tetracycline. The results suggested that this strain carries a plasmid (OCT) responsible for oil utilization which is quite stable and might be concerned with antibiotics resistancy.
Crude Oil-degrading Properties of Psychrotrophic Bacterium Acinetobacter calcoaceticus A1-1
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 74~81
To investigate the oil degrading properties of psychrotrophic bacterium Acinetobacter calcoaceticus Al-l the effects of environmental factors on this bacterium were studied. The optimal environmental conditions for cell growth rate and oil-emulsifying activity were as follows; temperature
, pH 7.5, salt concentration 0- 3% and crude oil concentration 0.1%. Additionally the optimal concentration of Nand P source for cell growth rate and oil-emulsifying activity were 0.76 mM and 0.057 mM as
, respectively. Analysis of remnant oil by gas chromatography showed time dependent oil degradation pattern by A. calcoaceticus during cultivation; At lOoe and
e, most alkane peaks were disappeared and it showed large quantities of crude oil were degraded. But at
e alkane compounds in the crude oil were partially degraded even after 120 hours incubation.
Effects of Oxygen and Salt on the Growth of Bifidus and Anaerobic Bacteria Isolated from Korean Traditional Fermented Foods
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 82~87
Fourteen samples of kimchies and soy bean pastes were used to isolate strictly anaerobic bacteria on complex BL agar and on a selective BS agar for bifidus bacteria. About
colonies per g sample were developed on BL agar under strictly anaerobic conditions, while BS agar supported the growth of
colonies per gram sample at the same condition. All colonies developed on BS agar at anaerobic conditions grew in aerobic conditions and did not show fructose6-phosphate phosphoketolase activity. Type cultures of Bifidobacterium did not grow in PYG medium containig more than 3% NaCI. From these results it is conduded that salted fermented food cannot support the growth of strictly anaerobes induding Bifidobactenum.
Diversity of Actinomycetes - Species, morphology and life cycle -
Microbiology and Biotechnology Letters, volume 21, issue 1, 1993, Pages 88~94
Actinomycetes are the most diverse group of bacteria. in variety. morphology. and life cycle. Especially. the variety of genus and species of Actinomycetes is so diverse among other microorganisms that about 50 genus of Actinomycetes have been reported until now. Among them. Streptomyces genus is the most plentiful and over 220 species have been reported. The number of the genus of Actinomycetes will be expected to be fixed to about 55 genus and 250 Streptomyces species in the year 2000 thereabouts. Morphological and physiological characters of Actinomycetes varies with culture condition. and therefore diverse life cycles are also possible. In this paper. life cycles of the representative Actinomycetes, Nocardia, Streptomyces. Kitasatosporia. GeodermatoPhi/us. and Sporichthya. according to the condition of nutrient in the media were reviewed