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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 21, Issue 6 - Dec 1993
Volume 21, Issue 5 - Oct 1993
Volume 21, Issue 4 - Aug 1993
Volume 21, Issue 3 - Jun 1993
Volume 21, Issue 2 - Apr 1993
Volume 21, Issue 1 - Feb 1993
Selecting the target year
Effect of Dual Inoculation with Glomus mosseae and Rhizobium meliloti on Growth and Nitrogenase Activity of Medicago sativa L.
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 95~100
Effect of dual inoculation with vesicular-arbuscular(VA) mycorrhizal fungi, Glomus mosseae, and Rhizobium meliloti R455 on growth, nodulation, and nitrogenase activity of alfalfa (Medicago sativa L. cv. Vernal) were examined in pot experiment. After 63 days growth, shoot length, number of leaf, and leaf size of alfalfa were increased as as result of dual inoculation with Glomus mosseae and Rhizobium meliloti. Total dry weight of alfalfa plant was increased 1.4 times compared to single inoculation with Rhizobium meliloti. Nodule number and mean fresh weight of nodule per plant were also increased due to the mycorrhizal infection.
Protoplast Formation and Regeneration in Lactobacillus helveticus
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 101~106
The optimal conditions for the production and regeneration of L. helveticus protoplasts were examined. The protoplast formation of L. helveticus was most efficient obtained when the cells grown to mid and late logarithmic phase in MRS medium were used. The maximum number of protoplasts was obtained when lysozyme and mutanolysin were used to lysis the cell wall in 20mM HEPES buffer (pH 7.0) containing 1M sucrose. Regeneration was accomplished with a complex medium containing 10% sucrose, 10mM MaCl2, 20mM CaCl2, 5% gelatin and 0.5% bovine serum albumin. The regeneration frequency of the protoplasts was 10-20% after 5 days of incubation at 30C.
Studies on the Protoplast Fusion between Lactobacillus bulgaricus and Lactobacillus helveticus
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 107~112
The optimal conditions for protoplast fusion between the cells of kanamycin resistant Lactobacillus bulgaricus IFO 13593 and those of lincomycin resistant Lactobacillus helveticus IAM 12090 were investigated in this study. The highest fusion frequency of 9.1*10-4 was obtained when protoplast fusion was carried out for 0.5 min using 40% PEG 4000 solution as a cell fusion stimulant and subsequent post-PEG-incubation was undergone at 30 for 30 min in the PPI medium.
Cloning and Expression of
-l,4-Glucosidase Gene from Pseudomonas sp. in Escherichia coli and Bacillus subtilis
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 113~118
Fro the purpose of producing glouse from cellobiose or oligo saccharide and obtaining genetic information of beta-1,4-glucosidase gene, alpha beta-1,4-glucosidase gene of Pseudomonas sp. LBC505, potent cellulase complex and xylanase producing strain, was cloned in Esherichia coli and Bacillus subtilis into pUC19 and pBD64, respectively. Recombinant plasmid pGL1 contained 1.2kb EcoRI fragment was isolated from transformants forming blue color around colony on LB agar plate containing 20 ng/ml of 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside(X-glu) and ampicillin.
Screening of Alkalophilic Bacillus sp. for Overproduction of Cyclodextrin Glucanotransferase and Its Enzymatic Properties
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 119~124
An alkalophilic microorganism for overproduction of cyclodextrin glucanotransferase (CGTase) was newly isolated from hot-water spring soil, and identified as Bacillus firmus var. alkalophilus H609. The strain maintained stability during preservation and cultivation for the enzyme production, and produced significant amount of CGTase corresponding to the volumetric activity of 75 units/mL at 37C, initial pH of 11.2, and after 40 hours. The strain excreted several different proteins showing CGTase activity that catalyzed the formation of mainly beta-and Gamma-type cyclodextrin (ratio of 7:1) from soluble starch without accumulation of alpha-type. Other enzymatic properties were also investigated.
Isolation of Pseudomonas aeruginosa 90-2-2205 Producing the S-type Pyocin from Korean Patients and the Pyocin Production
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 125~131
The s-type pyocin, one kind of bacteriocins, is a bactericidal substabce of protein nature produced by certain Pseudomonas aeruginosa and is active against some other strains of the same or closely related species. Among many Pseudomonas aeruginosa strains collected from the patients at the Hospitals in Seoul and Taegu cities, some Pseudomonas aeruginosa pyocinogeny were determined by pyocin typing. As a result, Pseudomonas aeruginosa 90-2-2205 was selected as the S-type pyocin producing microorganism due to its highest antimicrobial and protease sensitivity.
Purification and Characterization of the S-type Pyocin of Pseudomonas aeruginwa 90-2-2205 Isolated from Korean Patients
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 132~138
The s-type pyocin was purified from the lysate of the mitomycin C-induced Pseudomonas aeruginosa 90-2-2205 cells by the other of ammonium sulfate precipitation, DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration. The purity was confirmed by the polyacry-lamide gel electrophoresis. The molecular weight of the purified pyocin was estimated 180, 000 by gel filtration. The pyocin was analyzed to be a complex of some polypeptides by the SDS-PAGE. The pyocin was stable by heat treatment and at pH 6-7.5 by adding 10-3% gelatin and 0.2M NaCl to the 10mM Tris-HCl buffer (pH7.5). Its killing action against the sensitive cells was assumably a single hit process.
Tyrosinase-Inhibiting Isoflavonoids Produced by Streptomyces sp. 20747
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 139~143
Three isoflavonoids having tyrosinase-inhibiting activity were isolated from the culture filtrate of Streptomyces sp. 20747. Their structures were determined by UV, EI-MS, 1H-NMR, 13C-NMR to be daidzein, daidzein 7-rhamnoside, and genistein 7-rhamnoside, which were competitive with substrate and had IC50 value of 14, 19, and 16 ng/ml, respectively to mushroom tyrosinase and did not inhibit melanin production of Streptomyces bikiniensis. Soybean meal as well as peptone were found to be a good nitrogen source for tyrosinase-inhibiting isoflavonoids production, susgesting that soybean meal is not the origin of tyrosinase inhibiting isoflavonoids formation in Streptomyces sp. 20747 strain.
Identification of Interleukin-l Like Material in Streptornyces Culture Supernatant
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 144~149
We have identified a T cell-activating material in the culture supernatant of Streptomyces species. The factor in microbial culture supernatant (MCS) induced thymocyte proliferation in a does dependent fashion and it could be detected by immunoblot analysis using anti-interleukin-1(IL-1) antibody. The factor in MCS was slightly larger(about 21 kd) in its molecular weight than IL-1 on SDS-PAGE. When 125I-MCS was covalently coupled with homo-bifunctional cross-linking agent, disuccinimidyl-propionate to IL-1 receptor(IL-1R) on mouse thymoma cell(EL-4) and immunoprecipitated with anti-IL-1R antibody the molecular weight of this complex of 110 kd was observed.
Enzymatic Properties of the 2,3-Dihydroxybiphenyl Dioxygenase Purified from Pseudomonas sp. DJ-12
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 150~156
The 2,3-dihydroxybiphenyl(2,3-DHBP) dioxygenase, the product of pcbC gene, was purified from the biphenyl and 4-chlorobiphenyl degrading Pseudomonas sp. DJ-12 by the methods of acetone precipitation, DEAE-Sephadex A-50 ion exchange chromatography, and Sephadex G-150 gel filtration chromatography. The enzyme was estimated to be about 260 kilodaltons in molecular weight and to be consisted of eight subunits. The Km value of the enzyme was 61 nM to 2,3-DHBP and the highest activity of the enzyme was observed at pH 8 and 30C.
Evaluation of Operation Condition and Power Consumption of the Rotating Drum Type Bioattritor for Direct Saccharification of Raw Starch
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 157~162
Raw starch can be effectively saccharified in an enzyme reaction system containing sttrition-milling media. In order to develop an effcient attrition-coupled bioreactor(bioattritor), a rotating drum type bioattitor was construced, and its optimal operation conditions and power consumptions were evaluated. The optimal conditions for 3l bioattritor were 4 baffled, baffle size of 1:0.05 (the ratio of drum diameter to baffle), drum rotation speed of 100 rpm, and 1.33g of 3 mm glass bead/g of raw corn starch.
Effect of Substrate Micellization on the Hydrolysis Rate of Phospholipid by Phospholipase
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 163~170
The effect of substrate micellization on the hydrolysis rate in the production of lysopho-sphatidylcholine (LPC) from phosphatidylcholine (PC) using hog pancreas phospholipase A2(PLA2) was studied. The optimal temperature and pH for the reactions in aqueous phase was found 42C and 7.2, respectively. For a given PC concentration, initial reaction rate was progressively increased with the addition of sodium deoxycholate (DOC), which could transform the bilayer of phospholipids into micellar structure.
Isolation and Identification of Antioxidant from Oriental Therapeutic Drug for Hepatitis B
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 171~175
The antioxidant in the oriental drug, NP-S, traditionally used as a therapeutic agent for Hepatitis B was identified. The NP-S was divided into two fractions at molecular weight cut-off of 1000 by ultrafiltration and the fraction having molecular weight less than 1000 was named as NP-S(l)10. Both NP-S and NP-S(l)10 had antioxidant activity. An antioxidant that was extracted by chloroform from water-insoluble fraction in lyophilized NP-S(l)10 had a little smaller activity than NP-S(l)10 and identified as cyclooctasulfur(S8) by mass spectrum.
Heat Resistance of Vegetative and Starved Listeria monocytogenes Sott A
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 176~180
Survival and heat resistance of Listeria monocytogenes Scott A in various nutritional environments and cell type on stainless steel were determined. Viable cell of L. monocytogenes Scott A was most rapidly decreased in phosphate buffer among various media such as NSM (30 g TSB/1 l D.W.), LNM (2 g TSB/1 l D.W.) and phosphate buffer (pH=7) during incubation at 21 and 35C but survived for 15 days at 21C. Vegetative and starved L. monocytogenes Scott A were survived after heat treatment for 5 min at 65C while not detected at 72C.
The Function of Two n-Alkane Inducible Genes (ALIl, POX18Cm) for n-Alkane Assimilating Candida maltosa
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 181~186
The functions of n-alkane inducible genes, ALI1 and POX18Cm isolated from Canida maltosa were investigated, using it's distruptants. As a result, it is suggested that ALI1 is essential for n-alkane assimilation in C. mltosa and it regulates genes related to assimilation of n-alkane (ALI1, P450alk POX18Cm) at transcriptional level. Nuclear localization experiments indicated that ALI1 was located and functioned in the nucleus. POX18Cm is considered as a peroxisomal nonspecific lipid transfer protein gene related to n-alkane assimilation in C. maltosa also regulated by ALI1. But it had no significant effect on n-alkane assimilation in C. maltosa.
Antimicrobial Activity of Some Medical Herbs and Spices against Streptococcus mutans
Microbiology and Biotechnology Letters, volume 21, issue 2, 1993, Pages 187~191
In order to find out an anticariogenic substance, antibacterial activities of some medical herbs and spices against Streptococcus mutans were investigated. The essential oils of oregano, thyme, sage, fennel, nutmeg, rosemary, calamus and cassia cortex were shown antibacterial effect against S. mutans. The essential oil of oregano among other tested medical herbs and spices showed the most active antimicrobial activity. The essential oil of oregano inhibited the activity of glucosyltransferase from S. mutans. The minimal inhibition concentration of oregano essential oil was determined as 0.05nl/ml. The activity of glucosyltransferase from S. mutans was inhibited in the presence of oregano essential oil.