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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 21, Issue 6 - Dec 1993
Volume 21, Issue 5 - Oct 1993
Volume 21, Issue 4 - Aug 1993
Volume 21, Issue 3 - Jun 1993
Volume 21, Issue 2 - Apr 1993
Volume 21, Issue 1 - Feb 1993
Selecting the target year
The Characteristics of Bacteriophage-resistant Lactococcus lactis subsp. cremoris ATCC 11602-A1
Lee, Chun-Hwa ; Kang, Kuk-Hee ; Bae, In-Hyu ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 293~298
The ppage resistance mechanism of Lactococcus lactis subsp. cremoris ATCC 11602-A1 was investigated. When parent and A1 were incubated at 30 and 40
, A1 grew well and multiplication of phage(MOI=1)on A1 slightly occurred at 40
in contrast with parent. There was a great difference of proteolytic activity between parent and A1, irrespective of the temperature. As a result of ADS treatment oon culture broth, survival rate of A1 was 27% at the lethal concentration of parent and adsorption rate of phage was increased to 95~97%, which was considered to come from the exposure of phage receptor site masked by an unknown component. These results suggest that acridine orange (AO) treatment leads to the modification of cell wall, conferring resistance to high temperature and lytic phage. No change in plasmid profiles of A1 at 30 and 40
were found, which suggests that plasmid is not relative to temperature-resistance of A1.
Numerical Identification of a Streptomyces Strain Producing a Thermotolerable Restriction endonuclease Svi I
Bae, Moo ; Yun, Mi-Sub ; Kim, Hyoung-Tae ; Lee, Kye-Joon ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 299~305
Numerical identification was carried out for an isolate of Streptomyces D2-5 producing a new restriction endonuclease Svi I. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces violochromogenes in the major cluster 18 of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces violochromogenes.
Effect of Natural Oil on the Mycelial Growth of Flammulina velutipes
Ahn, Jang-Hyuk ; Lim, Wang-Jin ; Song, Chi-Hyeun ; Yang, Han-Chul ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 306~309
Vegetable oils supplemented to the basal medicum stimulated mycelial growth of Flammulina velutipes. The mycelial yield was increased 3.5 folds by addition of 3% (v/v) ricebran oil. Maximum mycelial yield (18.2mg/ml) was obtained by addition of 3.0% ricebran oil with 1.0%
to the basal medium. There was no significant difference between the liquid and solid spawn in the yield of sporophores.
Functional Properties of a Interspecific Protoplast Fusant from Lactobacillus bulgaricus and Lactobacillus helveticus
Jun, Hong-Ki ; Park, Hyun-Jeong ; Song, Jae-Chul ; Baik, Hung-Suk ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 310~315
An interspecific fusant was made from the protoplasts of two strains of Lactobacillus genus (L. bulgaricus and L.helveticus). And in order to test the applicability of the fusant in manufacturing the cheddar cheese, functional properties of the strain was examined by determining acid-producing activity, three important enzyme activities and volatile free fatty acid-producing activity. The recombinant strain did not exhibit greatly increased acid-porducing activity. Lipase and volatile free fatty acid-porducing abilities of the fusant, however, were remarkably higher than those of the two parental strains. The fusant actually porduced the cheese porduct of the highest ammount of total volatile free fatty acid after 7 days ripening at 10
. Finally, the cheddar cheese ripened with this strain was also evaluated to be high preference and flavor intensity by organoleptic panel tests.
Antitumoral Compound , MCH-201 , an Effector on Proliferation and Morphology of Human Breast Tumor Cell Line, MCF-7
Kim, Hang-Sub ; Kim, Se-Eun ; Kim, Young-Ho ; Lee, Sung-Woo ; Oh, Goo-Taeg ; Kim, Hwan-Mook ; Lee, Jung-Joon ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 316~321
MCH-201 was isolated from the mycelium of Streptomyces sp. Ba16 as a potent effector on proliferation and morphology of human breast tumor cell line, MCF-7. Morphological change could be observed at concentration between 2.5
g/ml and 250pg/ml and showed cytotoxic effect at the concentration of more than 5
g/ml. This compound also showed inhibitory effect on DNA synthesis of hepatoma cells, Hepa 1c1c7, and strong cytotoxic effect on proliferation of human tumor cell lines, A549 and XF498.
Concanamycin B, Active substance Against Phytophthora capsici Produced by Streptomyces neyagawaensis 38D10 Strain
Kim, Chang-Jin ; Lee, In-Kyoung ; Yun, Bong-Sik ; Yoo, Ick-Dong ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 322~328
During the screening of antifungal compounds from microbial secondary metabolites to control phytophthora blight of red pepper caused by Phytophthora capsici, a soil isolate, strain 38D10 was selected. Based on taxonomic studies, this strain was identified as Streptomyces neyagawaensis. The antifungal compound was purified from culture broth by HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, HPLC and identified as concanamycin B by UV.
-NMR, SIMS analysis. Concanamycin B has strong antifungal activity against some phytopathogenic fungi but not antivacterial activity and preventive value were 50% and 100% at 125ppm and 250ppm in pot assay.
Formation and Production of Ascorbate Oxidase by Cucumber Tissue Cultured Cells
Lee, Jong-Hwa ; Chung, Ho-Kwon ; Shinmyo, Atsuhiko ; Lim, Bun-Sam ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 329~335
Ascorbate oxidase activity in various cucumber tissue extracts was highest in young fruit peeling. Cucumber callus was induced from young fruit peeling and callus cell lines were selected for more than 7 months, which porduced high levels of ascorbate oxidase and had a high growth rate. Induction of callus was optimized with Linsmaier-Skoog(LS) medium at 25
in dark phase. Ascorbate oxidase activity reached a maximum at 5 days after transfer to LS basal liquid-medium ant then declined. The enzyme activity in callus cells was stimulated by addition of 10
in the early logarithmic phase of growth. And also, adding 10
at 3rd day 7th day of culture period, ascorbate oxidase activity in callus cells was maintained to high level. Maximum yield of ascorbate oxidase was found at the 25th day by flask shaking culture, but three-fold of ascorbate oxidase activity was obtained at the 16th day by jar fermentation.
Enhanced Partitioning of Proteins in Metal-Affinity Aqueous Two-Phase Systems
Chung, Bong-Hyun ; Park, Young-Hoon ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 336~341
In metal-affinify aqueous two-phase systems, protein partitioning is affected by a variety of parameters such as pH, the number of surface-accessible histidines, and the amount and partition coefficient of metallated polythylene glyco(PEG) ligand. To enhance partitioning of proteins with surface-accessible histidines, we have synthesized and used a (Cu(II)-ininodiacetic acid)
-PEG20,000) as well as Cu(II)IDA-PEG5,000 as an affinity ligand. The partition coefficient of
-PEG20,000 in a PEG5,000/dextran two-phase system was 30.1, which corresponded to a 3.8-fold increase over that of Cu(II)IDA-PEG5,000. The partitioning experiments were performed on four proteins, horse cytochrome c, S. cerevisiae cytochrome c, horse myoglobin, and sheep myoglobin. Partitioning of proteins which convey surface-accessible histidines was enhanced dramatically by the addition of
-PEG20,000 ligand. These results demonstrate that enhanced partitioning of metal-binding proteins in an aqueous two -phase system can by achieved by using an appropriate metallated PEG ligand.
Kinetics of producing
-carotene from Dunaliella salina by Light Limited Turbidostat Cultivation
Park, Young-Shik ; You, Ho-Keum ; Ohh, Shang-Jip ; Lee, Hyeon-Yong ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 342~347
It was proved that the cell growth followed a photo-inhibition model in light-limited turbidostat cultivation, having 1.06 (1/h) of maximum specific growth rate and 0.00094(kcal/
/h) and 0.063 (kcal/
/h) as half saturation and light inhibition constants, repectively.
-carotene production showed a growth related porcess. And the activation energy of Dunaliella salina was roughly estimated as 12.36 (kcal/mole) in employing Arrhenius relationship. It should also point out that relatively much porduction of
-carotene was observed at hight light intensity with yieding 1.04 (mg-carotene/g-dry cell/day) of specific product production rate while the cell growth was decreased. The optimal conditions of producing
-carotene in turbiodostat cultivation were as follows:
/h)of light intensity, 2 (mM) and 50(mM) of nitrate and sodium bicarbonate concentrations and 100(ml/h) of
Performance of a Novel Bioreactor Equipped with Moving Membrane Tube-Aeration System
Kim, Young-Nam ; Jeon, Byung-Cheol ; Lee, Jin-Ha ; Lee, Hyeon-Yong ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 348~353
The optimal conditions for operating a moving-aeration bioreactor were determined as 30rpm and 150 (ml/min) of air flow rate, which can yield ca. 7.3 (l/h)of maximum mass transfer coefficient. It was also found that the agitation speed played much much important role than air input rate in oxgen transfer into the medium.
(cells/ml) and 0.6 (ml/l) of maximum cell denisty and IL-2 production were observed in batch cultivation of IL-2 producing BHK cell line. 0.53 (mM/l/h) of oxygen uptake rate was also estimated. The performance of a moving-aeration bioreactor (specific growth rate and oxygen uptake rate, etc.) was superior to other culture systems, such as cell-life and static membrane aeration bioreactors. Ii must be useful to apply this reactor to many culture processes by improving structural limitations in scaling-up the system.
Control of dissolved Oxygen Concentration and Specific Growth Rate in Fed-batch Fermentation
Kim, Chang-Gyeom ; Lee, Tae-Ho ; Lee, Seung-Cheol ; Chang, Yong-Keun ; Chang, Ho-Nam ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 354~365
A novel control method with automatic tuning of PID controller parameters has been developed for efficient regulation of dissolved oxygen concentration in fed-batch fermentations of Escherichia coli. Agitation speed and oxygen partial pressure in the inlet gas stream were chosen to be the manipulated variables. A heuristic reasoning allowed improved tuning decisions from the supervision of control performance indices and it coule obviate the needs for process assumptions or disturbance patterns. The control input consisted of feedback and feedforword parts. The feedback part was determined by PID control and the feedforward part is determined from the feed rate. The proportional gain was updated on-line by a set of heuristics rules based on the supervision of three performance indices. These indices were output error covariance, the average value of output error, and input covariance, which were calculated on-line using a moving window. The integral and derivative time constants were determined from the period of output response. The specific growth rate was maintained at a low level to avoid acetic acid accumulation and thus to achieve a high cell density. The specific growthe rate was estimated from the carbon dioxide evolution rate. In fed-batch fermentation, the simutaneous control of dissolved oxygen concentration (at 0.2; fraction of saturated value) and specific growth rate (at 0.25
) was satisfactory for the entire culture period in spite of the changes in the feed rate and the switching of control input.
Continuous Rapid Fermentation of Soy Sauce by Immobilized Zygosaccharomyces rouxii BH-90 and Candida versatilis BH-91 Using Column Type Reactor
Ryu, Beung-Ho ; Cho, Kyung-Ja ; Chae, Young-Ju ; Jin, Seung-Heun ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 366~372
The aim of this study was to find out the rapid fermentation of soy sauce from koji hydrolyzate using column type reactor packed with immobilized yeast cells. Each immobilized cell of Zygosaccharomyces rouxii BH-90 and Candida versatilis BH-91 in the packed column type reactor produced 2.8% ethyl alcohol and 18mg/l 4-ethylguaiacol over 96 hours under the optimal condition. Continuous fermentation was performed by immobilized Z. rouxii BH-90 packed in column type reactor. Immobilized Z. rouxii BH-90 produced 2.30~2.85% ethyl alcohol during 30 days, and decreased gradually from 40 days to 80 days. Also C. versatilis BH-91 produced 4-ethylguaiacol at the constant rate of 16~18mg/l and decreased gradually after 40 days. Final product of soy sauce contained 2.8% ethyl alcohol and 18mg/l 4-ethylguaiacol. However, amino acid compositions of final products were consisted of predominantly glutamic acid, leucine, arginine, aspartic acid, lysine and valine, which were more than 50% of total amino acid.
Ethanol Production by a New Method of Alginate-Immobilization
Kim, Eun-Young ; Kim, Seung-Wook ; Kim, Keun ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 373~380
When the cells of yeast K35 were immobilized in Ca-alginate gel, cell concentration and viability decreased as alginate concentration increased. Considering the results, 2% (w/v) Ca-alginate concentration would be suitable. Among various concentrations of additives and cross-lin-king agent, the addition of 1.67% (w/v) of bentonite together with 0.33% (v/v) of glutaraldehyde (ABG bead) resulted in the highest ethanol production of 1.8%(w/v), using YPD medium containing 2% glucose. ABG bead seemed to be more resistant to phosphate ion than Ca-alginate bead. 0.33%(w/v) of phosphate was a proper concentration for the ethanol production by ABG bead. Scanning electron microscopic observation depicted that the immobilized cells on the bead surface were coated by alginate gel and that the cells in the internal bead were cross-linked with alginate matrix. When repeated-batch culture was performed with ABG bead for 40 days in a packed-bed reactor, ethanol concentration of about 90~110 g/l-gel was maintained. Cell viability was maintained around 70%, and outgrowing cell concentration was below 6.3% of total cell concentration. Consequently, the results showed that ABG head was a potential carrier for continuous production of ethanol compared to conventional Ca-alginate bead.
Development of Dermal Equivalent Using Mouse Fibroblasts
Yang, Eun-Kyoung ; Lee, Jae-Ho ; Choe, Tae-Boo ; Park, Jung-Keug ;
Microbiology and Biotechnology Letters, volume 21, issue 4, 1993, Pages 381~391
As the first stage of development of an artificial skin, fibroblasts were cultured in the collagen matrices to make a living dermal equivalent. Mouse embryonic fibroblasts were incorporated into a collagen matrices on plastic dishes containing concentrated DMEM culture media supplemented with sodium bicarbonate, hepes, antibiotics and fetal bovine serum. As the growth stimulation components, glycosaminoglycans were added: hyaluronic acid, chondroitin sulfate, heparin, chitosan were incorporated into the media at a concentration of either 1% or 5% w/w/ to collagen in order to investigate the effect on development of dermal equivalent. After the few days of incubation, gel matrics were contracted and firm dermal equivalent were formed. And the keratinocytes were cultured on top of dermal equivalent and make a three dimensional artificial skin tissue.