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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 21, Issue 6 - Dec 1993
Volume 21, Issue 5 - Oct 1993
Volume 21, Issue 4 - Aug 1993
Volume 21, Issue 3 - Jun 1993
Volume 21, Issue 2 - Apr 1993
Volume 21, Issue 1 - Feb 1993
Selecting the target year
Characterization of Mosquitocidal Bacillus thuringiensis Strain H9B
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 393~398
One strain of mosquitocidal Bacillus thuringiensis, H9B, was isolated from soil. The biochemical characteristics and flagella antigenicity of the strain H9B is similar to that of B. thuringiensis subsp. darmstadiensis. The delta-endotoxin of the strain H9B coincided with that of B. thuringiensis subsp. darmstadiensis strain 73E10-2 on agarose double immunodiffusion test. The delta-endotoxin of B. thuringiensis subsp. israelensis contains hemolysin fragment (28 kb) on SDS-PAGE when the delta-endotoxin was solubilized in alkali, while that of the strain H9B does not contain 28 kb protein.
Screening of Microorganisms having Antiviral Activity against HIV Virus from Soil
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 399~405
For screening of the antiviral agent from soil, about 520 strains of microorganisms were evaluated for their antiviral activity, About 6.9% of strains showed more than 95% antiviral activity against Herpes Simples Virus (HSV)-1. Two strains among 30 strains active against HSV-1 virus showed a quite strong activity against human immunodeficiency virus.
The Production and Characterization of Monoclonal Antibodies to the Major Polyhedra Inclusion Body of the Occluded Form of Hyphantria cunea Nuclear Polyhedrosis Virus
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 406~413
This study was performed to produce monoclonal antibodies to the major polyhedral inclusion body (PIB) antigen of the occluded form of Hyphantria cunea nuclear polyhedrosis virus (HcNPV). PIB proteinS were purfied from the Spodoptera frugiperda cell infected with HcNPVs. Using the purified PIB protein, BALB/C mice were immunized 3 times with 2 weeks intervals. The spleen were removed and fused with Sp2/0-Ag14, mouse myeloma cells.
Detergent Sensitivity of mrdA and mrdB Shape-Forming Mutants of Escherichia coli
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 414~420
Escherichia coli mrdAts and mrdBts mutants forming spherical cells at 42C, were employed to investigate the possible role of both inner and outer membrance structures in the determination of cell shape of gram-negative cells. Spherical cells, but not rod-shaped wild types, were specifically killed by anionic detergents, such as sarkosyl, sodium dodecylsulfate and sodium deoxycholate. From the spherical intact cells grown overnight at 42C, much more proteins were released by sakosyl.
The Production of Foreign Protein in Mouse L Cell
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 421~427
Some interleukin 6 (IL-60 transcription control factors were resported as the regulator of IL-6 expression. A nuclear protein bound to interleukin 1 (IL-1) responsive element in the IL-6 promoter region was named NF-IL6 (nuclear factor for IL-6). This NF-IL6 was known to be very imporant as a transcription factor for various immuno-protein as well as for IL-6. The human NF-IL6 genes were transfected into the mouse L cells under the metallothionein promoter (MT promoter) to establish a model system for the expression of foreign gene in the mammalian cell line.
Characterization of Two Type Crystal Proteins Produced by Transformed Bacillus thuringiensis NT0423
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 428~434
Cloning and expression of two different crystal protein genes from transformed Bacillus thuringiensis were investigated. B. thuringiensis NT0423 is toxic to both Lepidopterous and Dipte-rous larvae. The pCG5 vector carrying crystal protein genes (mosquitocidal and hemolytic activity) of B. thurigiensis subsp. morrisoni PG-14 was transformed into B. thurigiensis NT0423. Transformant has expressed two type crystals of bipyramid from NT0423 and ovoid from pCG5 in one cell.
Production of Glutathione by Candida sp. Mutant
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 435~439
For the overproduction of glutathione, Candida sp. mutant was isolated by the treatment with U.V. light. The highest glutathione production of Candida sp. mutant was obtained after shaking culture for 48 hours in the cullture medium containing glucose 1.5%(w/v), yeast extract 4.0% (w/v), KH2PO4 0.04%(w/v), biotin 5 ng/ml, and L-cysteine 0.04%(w/v). The optimal pH and temperature for the glutathione production were pH 6.0 and 25C, respectively.
beta-Glucosidase를 생산하는 균주의 분리 및 조효소의 특성
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 440~445
The fungi SFN 416 strain which produced a stable beta-glucosidase was isolated from nature and identified to Aspergillus niger. Optimal conditions of enzyme reaction were temperature 36C, pH-5.0, reaction time-40 minutes. The enzyme was stable below 60C and in the range of pH 4.5-6.5. The enzyme was greatly inhibitied by Ag+ and slightly activated by Ca2+ (0.5mM) and Cu2+ (5 mM).
Purification and Characterization of Cholesterol Oxidase from Bacillus sphaericus
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 446~452
The cholesterol oxidase produced from Bacillus sphaericus was purified and characterized. Through a series of purification procedures including DEAE-Toyoperal 650C, Sephadex G-200 and DEAE-Sephadex A-50 column chromatography, the purified enzyme was shown to have a specific activity of 0.179 units/mg protein having 31.8 fold purification and final yield of 12%. The molecular weight of the enzyme was estimated to be 47kDa and 47.tkDa by Sephadex G-200 chromatography and SDS-PAGE. The optimum temperature and pH for the enzyme were 30C and 6.0, respectively. The activity of the purified cholesterol oxidase was inhibited by Fe2+ and Hg+.
Genetic Transformation of Bacillus subtilis by the Bacteriolytic Enzyme from Alkafophilic Bacillus sp.
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 453~460
The extracellular bacteriolytic enzyme from alkalophilic Bacillus sp. YJ-451 was endopeptidase which hydrolyzes the peptide bond at the amino group of D-glutamic acid in the peptidoglycan. Protoplast transfomation system of B. subtilis by the lytic enzyme that differs, in mechanisms, from lysozyme which was used to transformation of B. subtilis was investigated. High protoplast yield was obtained from cells cultured in PAB at the late logarithmic growth phase.
Transglycosylation Reaction of Cyclodextrin Glucanotransferase in the Attrition Coupled Reaction System using Raw Starch as a Donor
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 461~467
Transglycosylation reaction of cyclodextrin glucanotransferase (CGTase) was analyzed in the attrition coupled heterogeneous reaction system using raw starch as a donor` and mono-, di-saccharide, and glycoside as acceptors. For transglycosylation reaction of stevioside, the transglycosylation rate was similar and the transglycosylation yield was increased compare with conventional process using liquefied starch as the donor. Also the accumulation of maltooligosaccharides in reaction mixture was minimized.
Production of Casein Phosphopeptides by Protease from Streptococcus sp.
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 468~472
For the production of Casein Phosphopeptide(CPP) inhibiting the insolubility of calcium, 10% sodium caseinate was treated with 1.5% of protease from Streptococcus sp.. Optimal conditions and productivity for the CPP production, and properties of the CPP were compared with tryptic hydrolysates of sodium caseinate. Optimum conditions of pH, temperature and reaction time were 8.0, 50C, 4 hrs, respectively. Under these conditions the productivity of CPP was 23% and Molecular weight of CPP was ranged from 3, 000 to 17, 000. The results also showed that the insolubility of calcium was completely inhibited by using 1.5 times of CPP for the amount of calcium.
Hormonal Regulation of Glycerol-Phosphate Acyltransferase Gene Expression
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 473~477
Both glycerol-phosphate acyltransferase (GPAT) and 7.2 kb mRNAs were present at the highest level in liver. Glycerol-phosphate acyltransferase and 7.2 kb mRNA levels increased dramatically when fasted mice were refed a high carbohydrate diet. In mature 3T3-L1 adipocytes, insulin increased both glycerol-phosphate acyltransferase and 7.2kb mRNA levels 2.6 to 3-fold while dibutyryl cAMP decreased mRNA levels by 50% and 80%, respectively. These results indicate positive regulation by insulin and negative regulation by dibutyryl cAMP of both glycerol-phosphate acyltransferase and 7.2 kb mRNA.
The Production and Properties of Exopolysaccharides(P0L-11) by Bacillus sp. LK-1
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 478~485
The strain which produced highly viscous exopolysaccharides (EPS) in liquid culture was selected from soil. The strain was supposed to Bacillus sp. from the results of mophological, biochemical and physiological tests. The medium composition for EPS production was trypton 0.75%, sucrose 4%, CaCO3 0.01%, Winogradsky's nitrogen free mineral medium 5ml/l and pH 7.0. In 2-l jar fernenter, the viscosity of culture broth after 120-hr cultivation time was very high (60, 000 cps) and the amount of EPS was 6.2g/l.
Bioflocculant production from Bacillus sp. A56
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 486~493
A gram(+) bacteria that produced microbial flocculant was isolated from soil and classified as a Bacillus species and named as Bacillus sp. A56. The culture conditions of the strain for fluocculant production were studied in a shake flask. Optimum temperature and initial pH for flcculant production were 30C and 6.5, respectively. The optimized medium has gollowing composition: glucose 20g/l, NH4NO3 0.5g/l, K2HPO4 1.0g/l, KH2PO4 0.8g/l, MgSO4.7H2O 0.2g/l, MnSO4.4-6H2O 0.3g/l, CaCO3 0.5g/l, yeast extract 0.3g/l, tryptone 0.3g/l in tap water.
Production of Pure Pullulan from the Pigment-Deficient Isolate of Aureobasidium pullulans GM21
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 494~503
A fungal strain was isolated as a pullulan-producer from plant leaves and identified as Aureobasidium pullulan GM21. With A. pullulans GM21, culture conditions were optimized for the pullulan production and the changes of the molecular weight of pullulan produced were investigated according to the culture conditions we obtained maximum conversion yield of pullulan about 58-60%(40.8-42.0g/l) from 7% sucrose at 25C, initial pH 7.5 by the batch cultivations either in Erlenmeyer flask or in jar fermentor.
Effect of AcNPV Infection Conditions on Recombinant Protein Production in Spodoptera frugiperda 21 Cells
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 504~510
The effect of AcNPV infection conditions such as serum concentration, pH, CaCl2, lysosomotropic agent, cell density at infection, agitation, aeration and nutritional supplementattion on recombinant protein production in Spodoptera frugiperda 21 cells was investigated using tissue culture flask, bottle and spinner flask. It was shown that serum, CaCl2, pH and cell density at infection affected recombinant production. The lysosomotropic agent did not significantly influence recombinant protein production.
Production of High Acetic Acid Vinegar by Single Stage Fed-Batch Culture
Microbiology and Biotechnology Letters, volume 21, issue 5, 1993, Pages 511~512
The production of vinegar containing high acetic acid concentration was carried in a single stage fed-batch culture. The initial and residual ethanol concentration were 50.0g/l and 5.0g/l, respectively, and the ethanol concentration was maintained from 5.0g/l to 10.0g/l during fedbatch culture. The fermentation temperature was decreased by 1C for every increase of 2.0% in acidity. The maximum productivity was 2.53g/l-hr and the acidity was 16.08% after 40 hours of acetic acid fermentation.