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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 21, Issue 6 - Dec 1993
Volume 21, Issue 5 - Oct 1993
Volume 21, Issue 4 - Aug 1993
Volume 21, Issue 3 - Jun 1993
Volume 21, Issue 2 - Apr 1993
Volume 21, Issue 1 - Feb 1993
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Degradation of Polyhedral Proteins of Nuclear Polyhedrosis Viruses in the Gut Juice of Several Lepidopteran Larvae
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 513~519
The alkaline protease in the polyhedra preparation of Spodoptera litura nuclear polyhedrosis virus was successfully inactivated by heating at 100C for 20 minutes. SDS-PAGE analysis indicated that heat inactivated polyhedra is composed of major proteins of 31kDa and presumptive its polymer protein of 62kDa. However, this polyhedra was converted into several smaller molecular weight proteins when treated with midgut juice, but not by treatment with heat-inactivated midgut juice.
Morphological Characterization and Culture Conditions of A White Mutant of Ganoderma Iucidum
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 520~526
A morphologically different form of Ganoderma lucidum was isolated from a cultivator's farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white wherase those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5.
-Aminolevlinic Acid Biosynthesis in Rhodocyclus gelatinosus KUP-74
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 527~533
Aminolevulinic acid(ALA) was shown to be synthesized via active pathways of either C4 or C5 ALA biosynthesis in cells of a photosynthetic bacterium, Rhodocyclus gelatinosus KUP-74, where the C5 pathway was appeared to be preferntially expressed in the cells. It was strongly suggested that L-glutamine might be utilized more effectively than L-glutamate to synthesize ALA via C5 pathway in this bacterium from the fact of relationship between the cellular uptake rates of glutamate and its Gamma-derivaties and corresponded ALA productivities in vitro and in vivo.
Nucleotide Sequence of
-Amylase Gene in the Yeasr Schwanniomyces accidentalis var. persoonii CBS 2169
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 534~541
The relationship between Schwanniomyces occidentalis CBS 2863 (formerly castellii) and CBS 1153 (formerly alluvius), and their variety persoonii was examined at alpha-amylase gene level. Using Sch. occidentalis alpha-amylase gene as probe, Sch. occidentalis alpha-amylase gene homologues were obtained from Sch. occidentalis CBS 1153 and Sch. occidentalis var. persoonii. The restriction analysis of these homologues showed that the restriction enzyme sites between Sch. occidentalis CBS 2863 and CBS 1153 was identical but different between these strains and Sch. occidentalis var. persoonii.
Molecular Cloning and Expression of the Acetyl Xylan Esterase Gene of Bacillus stearothermophilus in Escherichia coli
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 542~548
Bacillus stearothermophilus was shown to express multiple xylanolytic enzymes including acetyl xylan esterase. Genomic DNA of the strain partially digested with HindIII was ligated into the HindIII site of pBR322, and expressed in E. coli HB101 cells in order to clone the gene for acetyl xylan esterase. One transformant among 4000 screened formed a clear zone around its colony on the LB agar supplemented with 1.0% tributyrin. The functional clone harbored the recombinant plasmid pKMG5 with an insert of 5.1kb.
Cellulase Production from the Catabolite Repression Resistant Mutant of Pseudomonas sp.
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 549~555
The production of cellulase by Pseudomonas sp. LBC505 isolated was under the strict genetic and biochemical control mechanisms such as catabolit repression and induction. These biochemical control reduced cellulase production. Thus LBC505 was mutated to increase enzyme yields. Cells growth and cellulase production were inhibited by the addition of 2-deoxy glucose (2-DG), which is presumed to function as repressor for the selection of high cellulase yielding mutant.
Taxonomy, Purification and Physicochemical Properties of Novel Antifungal Antibiotics AF-011A
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 556~563
AF-011A is a novel lipopeptide with potent antifungal activity isolated from Pseudomonas cepacia AF6008 deposited as KFCC 10759. The compound was isolated from the fermentation broth by extraction with 50% isopropyl alcohol. Purification was effected by chromatography on Diaion HP-20, Alumina and C18 followed by HPTLC on silica gel. These techniques affored two closelt related compounds. AF-011A1 ans AF-011A2. The molecular weights of AF-011A1/A2 were determined by fast atom bombardment mass spectrometry(A1 m/z 1,215 : A2 m/z 1,199).
Biological Properties and Structural Analysis of Novel Antifungal Antibiotics AF-011A
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 564~569
AF-001A is a novel antifungal cyclic glyco-peptise isolated from Pseudomonas cepacia AF6008. AF-001A is a mixture of AF-011A1 and AF-001A2. Each compound contains glycine(1), serine(2), asparagine(1), 2,4-diaminobutyric acid(1), beta-hydroxytyrosine(1), xylose(1) and a methylene chain amino acid(1). Additionally, A1 contains one beta-hydroxyasparagine that is replaced with as asparagine in A2. AF-011A showed high in vitro antifungal activity against various animal and plant pathogenic fungi and caused no irritation on the skin of rabbits.
The Production of Glucose-1-phosphate from Sucrose by Leuconostoc sp.
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 570~576
For the production of glucose-1-phosphate from sucrose, bacteria having sucrose phosphorylase were isolated from Kimchi. Among them, JS-05, newly isolated strain having high activity of sucrose phosphorylase was selected and identified as Leuconostoc sp. The specific activity of sucrose phosphorylase of Leuconostoc sp. JS-05 was the highest when the strain was cultured at 25C for 20 hrs in the medium (pH 7.5) containing 10 g sucrose, 5g corn steep liquor, and 2.5g yeast extract per liter.
Producyion of Threonine Using Methanol Dehydrogenase and Serine Hydroxyltransferase in a New Methylotrophic Bacterium KJ29
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 577~581
The amino acid threonine was produced from glycine and ethanol in a reaction mixture using cell free extract of the methylotrophic bacterium isolated from soil and identified as mellthylo-bacterium sp. KJ29. Although the isolate could grow on carbon source other than methanol, only the cell free extract from the cells grown on methanol produced threonine. Methanol dehydrogenase (MDH) activity was present only in the cells grown on methanol when compared to the cells grown on heterotrophic substrates.
The Purification and Properties of
-Amylase from Schwanniomyces casrellii CBS 2863
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 582~587
The extracellular alpha-amylase was purified to homogenity from the culture filtrate of starch grown Sch. castellii CBS 2863. The purified enzyme was glycoprotein with a molecular weight of about 56 kDa. The pH and temperature optimum were 5.5 and 40C, respectively. The enzyme was fairly stable up to 40C and at acid pH range (pH 4.0-7.0). The apparent Km and Vmax of the enzyme toward starch was 1.0mg/ml and 100U/mg protein, respectively. The analysis of amino acid composition was found to be acidic protein. The amino acid sequence of N-terminal peptide consisted of Asp-Val-Ser-Ser-Ala-X-X-Thr-Arg-Ser-Glu-Ser-Ile-Tyr.
Purification and Characterization of D-Xylanase II from Penicillium verruculosum
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 588~593
Xylanase(1, 4-beta-D-xylan xylanohydrolase` EC 22.214.171.124) II was purified from Penicillium verruculosum by using the techniques of two anion exchange chromatographies, and gel filtration. The molecular weight of this enzyme was about 22, 000 as determined by SDS-electrophoresis. The enzyme showed hydropytic activity toward xylan but did not catalyze hydrolysis of Rho-nitrophenyl-beta-D-xylopyranoside, Rho-nitrophenyl-beta-D-glucopyranoside, Rho-nitrophenyl-beta-D-cellobiopyranoside, and celluloses such as Avicel, cotton, filter paper, carboxymethylcellulose.
Antitumoral Compound, MCS-202, an Effector on Proliferation and Morphology of Human Breast Tumor Cell Line, MCF-7
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 594~599
In the course of screening for microbial metabolites employing human cancer cell line, we identified a mycelial extract of Streptomyces sp. 1365, which are effective on growth inhibition and morphological change of MCF-7, human breasr cancer cell line. By repeased column chromatography and recrystallization process, yellow needle crystals were obtained as an active compound and identified as resistomycin by spectral analysis.
Screening of Microorganisms Having ACAT Inhibitor Activity from Soil and Characterization of AI-3, ACAT Inhibitor Produced by Streptomyces sp. A-3
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 600~608
About 1, 300 strains isolated from soil were evaluated for acyl-CoA:cholesterol acyltransferase (ACAT) inhibition activity. About 4.0% of actinomycetes and 3.6% of fungi showed greater than 50% inhibition activity, respectively. However, none of the isolated bacteria exhibited inhibition activity more than 50%. Among them, one Streptomyces sp. A-3 showed a higher ACAT inhibition activity in culture broth. Isolation of the ACAT inhibitor (AI-3) was achieved by Amberlite XAD-7 column, silica-gel column, Sephadex LH-20 gel-filtration and reverse phase HPLC.
Cultural Characteristics and Pilot Scale Fermentation for the Submerged Mycelial Culture of Lentinus dfodes
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 609~614
The optimum conditions for the submerged mycelial culture of Lentinus edodes SR-1 were elucidated to be incubation temperature of 25C, initial pH 4.0, agitation of 300 rpm, inoculation of 10.0%(v/v), and aeration of 1.0 v/v/m in TGY medium. The optimum c/n ratio and economic yield coeffcient for the submerged mycelial culture were 13.1:1 and 0.45 respectively. As the plant growth hormones test, SCM medium containing 0.5ppm of 2,4-dicholorophenoxyacetic acid increased mycelial yield in 1.1%, but 6-benzylaminopurine was not effective.
Media Optimization and Fed-Batch Fermentation for Riboflavin Overproduction by Ashbya gossypii
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 615~621
In order to maximize the riboflavin production by a mutant strain Ashbya gosspyii, the optimization of medium and fed-batch fermentation were performed. As carbon sources, glucose and soybean oil were necessary for the riboflavin overproduction. Optimal concentrations of glucose and soybean oil in the flask cultures were found to be 3.0% and 0.5%, respectively, in a complex medium containing corn steep liquor(CLS) 1%. Among the various organic nitrogen sources tested, CSL was the most effective one both for the cell growth and riboflavin overproduction.
Production of Photosynthetic Bacterial Cells of Rhodospirillum rubrum P17 from Soybean Curd Waste Water
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 622~627
Rhodospirillum rubrum P17 was used to investigate the pontential for the treatment of soybean curd waste and for the utilization of the biomass produced. The maximal biomass production and COD removal from the waste water were obtained at 30C, pH 7.0 under 2,500lux production and 50 rpm of agitation. The initial COD level of the soybean curd waste water was 3,240mg/l, and after 4 days of cultivation in batch culture, 3.46g/l of cells was obtained and COD level of the waste water reduced to 150mg/l (COD removal rate 95.4%).
Inhibitory Effects of Chinese Pepper on the Mutagenicity and the Growth of MG-63 Humman Osteosarcoma Cells
Microbiology and Biotechnology Letters, volume 21, issue 6, 1993, Pages 628~634
The inhibitory effects of various extracts from Chinese pepper on the mutagenicity and the growth of MG-63 human osteosarcoma cells were studied. Chinese pepper was extracted with methanol and then the methanol extract was further fractionated by using hexane, chloroform, ethyl acetate and butanol. The methanol extract of Chinese pepper revealed the strong antimutagenic activity on the aflatoxin B1(AFB1) and N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in Ames mutagenicity test and SOS chromotest.