Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 24, Issue 6 - Dec 1996
Volume 24, Issue 5 - Oct 1996
Volume 24, Issue 4 - Aug 1996
Volume 24, Issue 3 - Jun 1996
Volume 24, Issue 2 - Apr 1996
Volume 24, Issue 1 - Feb 1996
Selecting the target year
Characterization and Identification of Streptomyces SL20209 Producing Valistatin and des-Asp
-Amastatin, Two Inhibitors of Aminopeptidase M.
Ko, Hack-Ryong ; Chun, Hyo-Kon ; Chung, Myung-Chul ; Suh, Hyun-Hyo ; Kim, Hong-Joong ; Park, Yong-Ha ; Kho, Yung-Hee ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 1~8
Characterization and numerical identification were carried out for an actinomycetes SL20209. Morphological, cultural and physiological perperties of SL20209 which porduced valistatin and des-
-amastatin as inhibitors of aminopeptidase M were evaluated. The isolate was identified to be the genus of Streptomyces. Fourty-three taxonomic units were analysed by using a TAXON program. The isolate was classified into the major cluster 29 of Streptomyces and best-matched to Streptomyces griseoplanus.
Purification and Characterization of Degradative Enzyme of Dental Plaque from Streptomyces sp. Y9343
Kim, Seong-Joo ; Han, Hong-Keun ; Yoon, Jeong-Weon ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 9~18
Streptococcus mutans has been implicated as primary causative agents of dental caries by insoluble glucan (IG) in human and experimental animals. An attempt was made to search for the
-1,3 glucanase that degrades IG produced by S. mutans.
-1,3 glucanase was detected in the culture supernatant of microorganisms, which are isolated from soils on agar medium containing IG as a sole carbon source. This Streptomyces sp. hydrolysed IG produced by immobilized S. mutans and was named as Y9373. This enzyme required
-1,3 glucan (IG) as an inducer. The optimum conditions for enzyme production were studied. The enzyme was purified by 30~70%
precipitation, anion exchange chroma tography on DEAE-cellulose and gel filtration on Sepadex G-75. The purified enzyme has a specific activity of 7840.0 U/mg protein giving 32.1-fold purification and final yield of 0.53%. The molecular weight was estimated to be about 22.5 kDa by SDS-PAGE. The optimum pH and temperature for enzyme reaction were 6.5 and 37
, respectively and the enzyme was relatively stable at the temperature below 60
. The activity of purified enzyme was enhanced by adding
into the medium, whereas inhibited by adding
and SDS. The
-1,3 glucanase for IG were estimated to be 2.50 mM and 0.0431 mM/min, respectively. The thin layer chromatographic analysis of hydrolysates from IG with
-1,3 glucanase showed that glucose was the main product of reaction. This enzyme activity was about 14 times higher than marketing dextranase as preventive agent against artificial dental caries by S. mutans in TH medium including 5% sucrose after 30 minutes.
Isolation and Characterization of MT2617-2B, a Phospholipase C Inhibitor Produced by an Actinomycetes Isolate
Ko, Hack-Ryong ; Lee, Hyun-Sun ; Oh, Won-Keun ; Ahn, Soon-Cheol ; Kim, Bo-Yeon ; Kang, Dae-Ook ; Mheen, Tae-Ick ; Ahn, Jong-Seog ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 19~26
A phospholipase C (PLC) inhibitor (MT267-2B) was isolated from the culture broth of actinomycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR,
-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of
1 and PLC-
1 were 25 and 50
g/ml, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.
Isolation and Numerical Identification of Streptomyces Strains Producing Inhibitors of Fungal Cell Wall Synthesis
Suh, Won-Na ; Park, Jung-Hee ; Lee, Ji-Young ; Kim, In-Seop ; Lee, Kye-Joon ; Bae, Moo ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 27~36
The aim of the present research program was to identify and develop strains of actinomycetes producing antifungal antibiotics which inhibit cell wall biosynthesis. 860 strains of Actinomycetes were isolated from various soil samples. Three isolates, EMS4, EMP22, and L234 were selected as the strains producing antifungal antibiotics inducing abnormal morphology against Penicillium cyclopium, Cryptococcus laurentii, and Aspergillus flavus, respectively. Taxonomic unit characters of the strains were tested and the data were analyzed numerically using TAXON program. EMS4, EMP22, and L234 were indentified to be a member of Streptomyces lavendulae, Streptomyces willmorei, and Streptomyces aburaviensis, respectively.
Augmentation of antitumor activity of antitumor drugs in combination with Lactobacillus casei HY2782
Yoon, Sang-Kun ; Bae, Hyoung-Suk ; Kim, Gyung-Tae ; Baek, Young-Jin ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 37~43
Augmentation of antitumor activity of antitumor drugs in combination with Lactobacillus casei HY2782 (LC2782) was studied against Sarcoma-180 (S-180) and Lewis lung carcinoma (3LL). Antitumor drugs used in this study were 5-fluorouracil (5-fu) and cyclophosphamide (CP). The prolongation effect of LC2872 on the life span of mouse intraperitoneally implanted with S-180 was stronger than that of OK-432 and BCG, while the inhibitory effect of OK-432 and BCG on the growth of 3LL solid tumor was a little stronger than that of LC2782. Average survival rates of mice administrated LC2782, OK-432 and BCG were 192%, 141%, and 112%, respectively, when that of the control was 100%, Intralesional administration of 5-Fu, CP, 5-Fu+LC2782 and CP+LC2782 resulted in 93%, 69%, 99% and 73% inhibition rates against 3LL solid tumor proliferation. The combination therapy of 5-Fu or CP with LC2782 significantly prolonged the life span of S-180-inoculated ICR mice. Average survival rates of mice administrated 5-Fu and CP alone were 115% and 99%. Furthermore, survival rates of mice administrated 5-Fu and CP in combination with LC2782 were 226% and 244%, respectively.
Production of 3,4-dihydroxyphenyl-L-alanine by Using the
-Tyrosinase of Citrobacter freundii Overexpressed in Recombinant Escherichia coli.
Lee, Seung-Goo ; Ro, Hyeon-Su ; Hong, Seung-Pyo ; Lee, Kyu-Jong ; Wang, Ji-Won ; Tae, Dong-Nyeon ; Uhm, Ki-Nam ; Bang, Sang-Gu ; Kim, Young-Jun ; Sung, Moon-Hee ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 44~49
By using the
-tyrosinase of Citrobacter freundii KCT2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18
and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammoniumacetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300mM and 50mM, respectively. Although pyrocatechol showed the optimal concentration at 20mM, it was controlled between 20mM and 50mM to avoid the depletion of substrate during the enzymatic synthesis. Meanwhile the synthetic rate was improved about 20% when ethanol was included in the reaction solution. Based on above results, a reaction medium for the productin of L-DOPA was prepared and incubated with 1 unit/ml of
-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solutin intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6g/l of L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.
Characterization of Xylanase from an Hybird between Aspergillus oryzae var. oryzae and Aspergillus Nidulans 514 by Nuclear Transfer
Yang, Young-Ki ; Moon, Myeng-Nim ; Park, Hyung-Nam ; Lim, Chae-Young ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 50~58
Interspecific hybrids between Aspergillus oryzae var oryzae and A. nidulans 514 were obtained by nuclear transfer technique. Several autotrophic mutants isolated from conidiospores of the two strains were mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were
. From observation of genetic stability, conidial size, DNA content, and nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrids showed 1.1~1.4 fold higher xylanase activities than parental strains did. The xylanase of Aspergiilus sp. TAVD514-3 was purified and some of it's enzymatc characteristics were investigated. The enzyme was purified about 85 fold with an overall yield of 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and CM-sephadex A-50 ion exchange chromatography. The purified enzyme functions optimally at pH 9.0 and 80
. The enzymatic activity was increased by the presence of
The Relationship between Virginiae Butanolide C(VB-C) and Receptor in Virginiamycin Production
Kim, Hyun-Soo ; Hyun, Ji-Sook ; Yu, Tae-Shick ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 59~66
Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the productin of virginiamycin in Streptomyces virginiae. To further understand the mechanism of virginiamycin induction, we isolated three mutants from S. virginiae by N-methyl-N'-nitrosoguanidine (NTG) treatment. The characteristics of the three mutants were confirmed as follows: the mutant No. 1 delayed the production of the VB-C, receptor and antibiotics; the mutant No.3 hyperproduced receptor; the mutant No.4 failed to produce the VB-C. The addition of synthetic VB-C couldn't induce the production of antibiotics in the mutant No.1 due to delayed production of receptor, could provoke the production of larger amount of antibiotics than parental wild type strain in the mutant No.3 due to the presence of large amount of receptor, and could induce production of very small amount of antibiotics in the mutant No.4 due to the absence of VB-C. Antimicrobial spectrum and HPLC analysis of the mutant No.1 and No.3 suggested that the VB-C might have a specific ability to induce the production of virginiamycin M and S. These results imply that the VB-C has an ability to trigger the production of virginiamycin under receptor existence in S. virginiae.
Expression of an Active Adenylate Forming Domain of Peptide Synthetase
Kim, Yoen-Ok ; Kim, Ki-Young ; Lee, Seong ; Lee, Young-Haeng ; Yu, Byung-Soo ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 67~71
The plasmid pK8 was constructed to verify the existence of an adenylate domain in peptide synthetase by using pGC12. 1.2 kb fragment, coding tyrocidine synthetase 1 (123 kDa) was deleted, and 79.6 kDa one was expressed in Escherichia coli XL1-blue. The truncated multienzyme activated phenylalanine and substrate analogues with comparable kinetics as the over expressed synthetase. ATP-[
]PPi exchange reaction was measured for the enzyme assay.
Expression of Escherichia coli
-galactosidase Gene by New Transfer Vector of Baculovirus
Woo, Soo-Dong ; Kim, Woo-Jin ; Kim, Hye-Seong ; Jin, Byung-Rae ; Kang, Seok-Kwon ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 72~76
To investigate the expression efficiency of new transfer vector of Bombyx mori nuclear polyhedrosis virus (BmNPV), Escherichia coli lacZ gene was inserted into new transfer vector pBmKSK1, under the control of polyhedrin promoter and expressed in BmN-4 cells and larvae of silkworm, Bombyx mori. The recombinant virus containing lacZ gene was isolated from BmN-4 cells coinfected with transfer vectro pBmKSK1-LacZ and wild type BmNPV genome, and analysed by Southern blotting. The expression of
-galactosidase was characterized by SDS-PAGE, Western blotting and
-galactosidase activity assay. The results showed that the level of expression in silkworm larvae was higher than that of BmN-4 cells.
An Antifungal Compound Against Phytophthora capsici Produced by Streptomyces sp. 3D3
Yun, Bong-Sik ; Kim, Chang-Jin ; Lee, In-Kyoung ; Hiroyuki, Koshino ; Yoo, Ick-Dong ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 77~81
During the screening for the antifungal compounds against Phytophthora capsici causing phytophthora blight of red pepper, we isolated a strong active compound, bafilomycin
, produced by strain 3D3. The producing organism was identified as Streptomyces sp. based on taxonomic studies. The antifungal compound was purified from culture broth by HP-20 column chromatography, ethylacetate extraction, silica gel column chromatography and HPLC, and was identified as bafilomycin
by color reaction, UV and
-NMR spectral data analysis. Bafilomycin
showed strong antifungal activity against various phytopathogenic fungi.
A Herbicidal Nucleoside Compound isolated from Streptomyces tubercidicus ME-9189
Kim, Won-Gon ; Kim, Jong-Pyung ; Kim, Chang-Jin ; Yoo, Ick-Dong ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 82~86
Three thousand microbial strains collected from different sources were screened for herbicidal activity. A strain of ME-9189 showed herbicidal activity against Digitaria sanguinalis and Portulaca oleracea was isolated from a mountainy soil. Based on taxonomic studies, the strain was identified as Streptomyces tubercidicus. The active compound of ME-9189 was purified from the culture broth by charcoal, silica gel, sephadex LH-20 column chromatography and crystalization, consecutively. The ME-9189 compound was identified as tubercidin by spectroscopic methods of UV,
-NMR, and EIMS. In the bioassay, growth of radish shoot and root was inhibited by 50% with tubercidin treatment of 10 ppm, showing 2 times higher activity than that of herbicidin A and similar to that of toyocamycin.
Characteristics and Action Pattern of Alikaline Lipase from Serratia liquefaciens AL-11
Choi, Cheong ; Kim, Tae-Wan ; Ahn, Bong-Jeon ; Kim, Yung-Hwal ; Son, Jun-Ho ; Kim, Sung ; Choi, Hee-Jin ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 87~91
The optimum temperature and pH for the enzyme activity were 45
and 10.0, respectively. The enzyme was stable in a pH range of 5 to 10, and 62% of its activity was lost on heat treatment of 60
for 20 min. The activity of the purified enzyme was inhibited by
, and slightly activated by
-Chloromercuribenzoic acid, 2,4-dinitrophenol and
did not show inhibitroy effect on the lipolytic activity of the alkaline lipase but ethylenediaminetetraacetic acid inhibited the enzyem activity. This suggested that the enzyme have metal group in its active site. Sodium salts of bile acids stimulated the enzyme activity. Analysis of hydrolyzates of olive oil after the reaction revealed that Serratia liquefaciens AL-11 produced non-specific lipolytic enzyme.
Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Butyric Acid and Valeric Acid by Azotobacter sp.
Song, Hee-Ju ; Lee, Il-Seok ; Bang, Won-Gi ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 92~100
For the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(P(3HB-co-3HV)) from butyric acid and valeric acid, 10 strains of bacteria capable of producing P(3HB-co-3HV) were isolated from soil. Among them, the strain HJ-067 showed the best ability of producing P(3HB-co-3HV), and was indentified as a Azotobacter sp. For the production of P(3HB-co-3HV), the optimum concentrations of butyric and valeric acid were 3.0g/l, respectively. The most effective nitrogen source was
at an optimum concentration of 0.75g/l, which was equivalent to 21.36 in C/N ratio. Deficiency of the cationic metal ions (
) in the proguction medium had stimulating effect on P(3HB-co-3HV) accumulation, especially in the manganese. deficient medium. The optimum temperature for P(3HB-co-3HV) production was 27
and the optimum initial pH was 7.0. Under the optimum conditions, 1.82g/l of P(3HB-co-3HV) and 3.00g/l of dry biomass were produced after 36 hour cultivation, and the P(3HB-co-3HV) yield and HV% were 60.60% (w/w), 15.92%, respectively.
DNA Toposiomerase I Inhibitor by Streptomyces sp. 7489
Lee, Dong-Sun ; Ha, Sang-Chul ; Lee, Sang-Yong ; Kim, Jong-Guk ; Hong, Soon-Duck ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 101~104
During the screening of inhibitor of DNA topoisomerase I from microbial secondary metabolites, Streptomyces melanosporofaciens 7489 which was capable of producing high level of inhibitor was selected from soil. The active compound (7489-1) was purified from the culture broth by solvent extraction, silica gel column chromatography and HPLC. The inhibitor was identified as dibutyl phthalate by spectroscopic methods of UV,
-NMR, DEPT and EI-MS. 7489-1 showed a strong inhibitory activity against topoisomerase I with 10
Effect of Mugwort Extract on the in vitro Mutagenicity, Desmutagenicity.
Lee, Sung ; Kwon, Dong-Jin ; Yoo, Jin-Young ; Chung, Dong-Hyo ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 105~110
Mugwort has been known as a traditional substitutive foodstuff and as showing a physiologically beneficial function to a human being. Therefore, effect of mugwort extract in terms of mutagenicity and desmutagenicity was investigated to berify its function. Ethanol extract from mugwort did not exhibit any mutagenicity. On the contrary, inhibitory effects of the ethanol extract were observed on mutagenicity induced by aflatoxin
, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole(Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b]indole(Trp-P-2) and 2-nitroflourene(2NF) using Salmonella typhimurium reversion assay. On direct-acting mutagen(2NF, 3
g/plate), ethanol extract showed a slight inhibitory effect of 19.7~22.9%, however on indirect-acting mutagen such as AFB1(2
g/plate) and Trp-P-2(1
g/plate), we observed higher inhibitory effect of 47.9~61.2%, 64.1~70.7%, 67.4~78.7%, respectively. Step-wise fractionation of the ethanol extract was done by using hexane, chloroform, ethyl acetate and water to obtain effective fraction. Among them, hexane, chloroform, and ethyl acetate fractions showed high inhibition of 63.0~80.0%, 77.5~82.1%, and 68.5~83.1%, respectively on the mutagenicity of
in Sal. typhimurium TA98. Consequently, these results indicated that mugwort extract contains some compound(s) which may show desmutagenicity.
Production Conditions and Characterization of the Exo-biopolymer Produced by Submerged Cultivation of Ganoderma lucijum Mycelium
Lee, Shin-Young ; Kang, Tae-Su ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 111~118
For the screening and the development of the new bio-material, cultural conditions for the exo-biopolymer (EBP) production throught the submerged cultivation of Ganoderma lucidum mycelium were investigated. Also, the fractionations and the purifications of the exo-biopolymer were carried out and the chemical compositions of the exo-biopolymer were examined. The optimal culture conditions for the exo-biopolymer production were pH 5.0, 30
and 100 rpm of agitation speed in the medium containing of 5% (w/v) glucose, 0.5%(w/v) yeast extract, 0.1% (w/v) (
, and 0.05% (w/v)
. In the flask cultivation for 7 days under these conditions, the concentration of the maximum exo-biopolymer and the cell mass were 15.4g/l and 18.8g/l, respectively. The specific growth rate was 0.039
. In addition, the substrate consumption rate, and the exo-biopolymer production rate were 0.043
, respectively. The exo-biopolymer was fractionated into BWS (water soluble exo-biopolymer) and BWI (water insoluble exo-biopolymer) by the water extraction, and the sugar contents of two fractions were higher than 97% (based on dry basis). The components sugar of BWS and BWI fractions were glucose, galactose, mannose, xylose, and fucose. Their molar ratios were 3.6:1.5:2.1:0.5: trace and 2.9:3.1:2.0:1.6:0.3, respectively.
Development of Enzyme-Linked Immunosorbent Assay for the Detection of Fumonisins
Shon, Dong-Hwa ; Hahn, Seong-Min ; Lim, Sun-Hee ; Lee, Yin-Won ; Cho, Sun-Hee ; Kang, Shin-Young ; Lee, Kyung-Ae ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 119~125
In order to develop enzyme-linked immunosorbent assay (ELISA) for fumonisins, production of specific antibodies, establishment of ELISA conditions, and quantitation of the toxin from spiked corns by ELISA were performed. Fumonisin
conjugated to cholera toxin (CT) with or without Freund's adjuvant was subcutaneously injected into 2 groups of rabbits. When the titer of the antisera produced by each rabbit was tested, higher titer was observed in case of the immunization with the adjuvant. By use of the antiserum showing the highest titer (1:16,000) and its purified antibodies, competitive indirect and direct ELISA's (ciELISA and cdELISA) were established, respectively. When the cross-reactivity of the antibody against fumonisin analogs was investigated by the ciELISA, it was very low against
(2%) but high against fumonisin
(179%). The sensitivity of the ELISAs was also very high, because the detection limit for
was 0.03 ppb in ciELISA and 0.3 ppb in cdELISA. When the ELISA's were applied to the spiked corns after extraction with 75% methanol, the assay recovery of
was too unstable to assay. However, when cleanup by strong anion exchange (SAX) cartridge was introduced to remove interfering materials, the mean ELISA recovery of
from corns spiked to 3~10 ppm was found to be 34.0% and stable (mean of CV, 8.2%).
The Production of Tissue Type-Plasminigen Activator and Mechanism of Cell Death from Human Promyelocytes(HL-60) in Low Serum Containing Medium
Kim, Hyun-Gu ; Sung, Ki-Don ; Kim, Tae-Ho ; Ahn, Ju-Hee ; Ham, Moon-Sun ; Park, Jin-Seo ; Lee, Hyeon-Yong ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 126~131
HL-60 was cultivated to produce tPA (tissue-type plasminogen activator) and study the mechanism of cell death. Maximum cell density and tPA production were obtained as
cells/ml and 324ng/ml, respectively under perfusion cultivation. tPA production was enhanced to 420ng/ml in adding 160nM of phorbol ester. The cells were gradually differentiated to granulocytes rather than proliferation. By Fluorescent microscope, apoptosis was prevailed except the death phase and in high agitation speed, but necrosis was prevailed in thawed cells and during the latter periods of the cultivation. It was also proved that tPA was most produced in apoptosis. To obtain higher tPA productivity, the cells must be maintained in apoptosis, not necrosis phase when the cells were dying.
Construction of a Computation Web Server for Genome Analysis
Park, Kie-Jung ; Lee, Byung-Wook ; Park, Yong-Ha ;
Microbiology and Biotechnology Letters, volume 24, issue 1, 1996, Pages 132~136
A comutation server is needed to provide analysis programs to Korean biologists, especially genome researchers, on GINet. For each analysis program, we implmented an input form with HTML and a CGI program for interface between an input form and an analysis program with C language on GINet computatin Web server. We made two construction methods of CGI programs for analysis programs, and implemented all CGI programs based on the methods followed by modifying each CGI program for specific processing of each program. On the server ten programs are availabel now, which include most frequently used ones and those developed by our team, and most programs with will be ported or developed by our team will be available on the Web server.