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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 27, Issue 6 - Dec 1999
Volume 27, Issue 5 - Oct 1999
Volume 27, Issue 4 - Aug 1999
Volume 27, Issue 3 - Jun 1999
Volume 27, Issue 2 - Apr 1999
Volume 27, Issue 1 - Feb 1999
Volume 9, Issue 6 - 00 1999
Volume 9, Issue 5 - 00 1999
Selecting the target year
Antimicrobial Activity of The Volatile Components from Fruit Peel of Chopi(Zanthoxylum piperitum DC)
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 179~183
The volatile components (essential oil) showing antimicrobial activity were extracted from the fruit peel of Zanthoxylum piperitum DC by distillation and separated by thin layer chromatography (TLC). The crude volatile components exhibited antimicrobial activity only at very high concentration. The active fraction obtained by TLC inhibited noticeably the growth of bacteria. The minimum inhibitory concentration (MIC) of the fraction were 150ppm, 300ppm, and 300ppm against Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis, respectively. The components in the active fraction were identified by gas chromatography/mass spectrometry to be geranlyl acetate (60.23%), citronellal(36.01%), citronellol(3.77%), geraniol(0.46%), and cumin ldehyde(0.43%).
Screening and Taxonomic Charactrization of D-Amino Acid Aminotransferase-producing Thermophiles
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 184~190
To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two thermophiles, Bacillus sp. Lk-1 and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-01, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.
Selection and Identification of Promicromonospora sp. KH-28 Producing Chitinase and Antifungal Antibiotic
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 191~196
A multifunctional antagonistic bacterium, producing both antifungal antibiotic and chitinase that could be used as biocontrol agents against fungal plant pathogens was isolated from the plant-disease suppressive soil. The isolate was identified as Promicromonospora sp. KH-28 from various morphological, biochemical and physiological tests. The antifungal antibiotic produced by Promicromonospora sp. KH-28 was soluble in n-butanol, methanol, toluene, n-hexane, ethanol but insoluble in H2O, acetone, chloroform, ethylacetate and ethylether. It inhibited the growth of fungal plant pathogens of Fusarium solani, F. oxysporum, Alternaria mali and Phytophthora capsici. The antagonistic Promicromonospora sp. KH-28 produced optimally the antifungal antibiotic when it was cultivated at pH 7, 3
for 5 days.
Antimutagenic Effect of Tansen (Salvia miltriorrhiza Bunge)
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 197~202
To confirm the effects of binlang(Areca catechu L.) and tansen(Salvia miltriorrhiza bung) on the mutagenicity induced by hydrogen peroxide, SOS Chromotest with Escherichia coli PQ37 and Ames test with Salmonella typhimurium TA104 were perlormed. Methanol-soluble parts of their water extracts showed high inhibitory effect against the mutagenicity of hydrogen peroxide in two bacterial mutation assays. Step-wise fractionation of methanol-soluble part from tansen was done using ethyl acetate, butanol and water. Among these fraction was further partitioned by Sephadex LH-20 column chromtography, and 6 subfractions were obtained. The fraction III showed the strongest inhibitory effects against the mutagenic activities induced by hydrogen peroxide. The inhibition rates of fraction III at concentration of 500
/assay were 28%, 30% and 15% against 4-NQO, MNNG and B(a)P, respectively. But the mutagenic potency of AFB1 was increased.
Properties of an Extracellular Amylase Produced by the Marine Halophilic Bacterium Vibrio alginolyticus
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 203~207
V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45
. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.
Immobilization of Agarase for the Agarooligosaccharide Production
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 208~214
The condition for immobilization of the partially purified agarase from Bacillus cereus ASK202 and the properties of the immobilized enzyme have been investigated. Agarase was immobilized on various supports by entrapment method. The enzyme immobilized on Na-alginate bead showed the highest activity among those studied. The optimal reaction conditions of the immobilized agarase were obtained in 3%(w/v) Na-alginate for the matrix, bead diameter of 2.5mm, 1 unit of agarase solution and 1.0%(w/v) calcium chloride solution. The optimum pH and temperature of the immobilized agarase were pH and temperature of the immobilized agarase were pH 7.0 and 4
, respectively. Km and Vmax values were 0.5mg/ml.min, respectively. The immobilized agarase conerted agar to agarobiose, and their total conversion ratio under the optimal condition was 89%.
Purification and Characterization of Aminoglycoside-Resistant inhibitior from methylotrophic Actinomycetes
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 215~222
Methylotrophic actinomycetes No. 155 produced an aminoglycoside antibiotics(AG)-resistant inhibitor. We have previously reported that the inhibitor shows strong inhibition to sisomicin-resistant strain. In order to understand the functions of inhibitor and sisomicin-resistance, characterizations and purification of inhibitor were investigated. Strain No. 155 was tentatively identified as Nocardiopsis sp. based on morphological and some physiological characteristics. In the antimicrobial activity test, the addition of inhibitor to sisomicin showed a reduction effect of MIC on the test strains such as Gram(+), Gram(-) bacteria and yeasts. The combination of the inhibitor and various antibiotics revealed synergistic against E. coli K-12 and B. subtilis PCI 219. The induced intracellular proteins from sisomicin-resistant strain exhibited the sisomicin inactivation by invitro test. And the induced intracellular proteins were inactivated by addition of the inhibitor. The inhibitor compound was purified by anion exchange chromatography(Dowex-1) and HPLC using Asahipak ES-502C column. The purified inhibitor compound was detected in a single peak(above 98.5% purity) through the HPLC analysis.
Optimization of Fermentation Processes with Singular Approximation and Minimum Principle
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 223~229
The two optimal control algorithms, singular approximation and minimum principle, were compared in this paper. The switching time with singular approximation was determined with mathematical derivation and the optimal control profile of specific growth rate was also calculated with minimum principle. The optimal control profiles were calculated by making simple model correlating the specific cell growth rate and specific product formation rate. The optimal control profiles calculated by singular approximation approach were similar to stepwise form of those calculatd by minimum principles. With the minimum principle, the product concentration was 8% more than that of singular approximation. This performance difference was due to a linearization of a nonlinear function with singular approximation. This optimal approaches were applicable to any system with different optimal cell growth and product formation.
Effect of Reducing Agents on Bifidobacterium Fermentation of Saccharified Rice Solution
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 230~235
This study was intended to develop a new rice product by the fermentation of saccharified rice solution using Bifidobacterium and to select an appropriate reducing agent to provide the anaerobic condition for the growth of Bifidobacterium during fermentation. The enhancement of the growth of Bifidobacterium in saccharified rice solution was achieved by the treatment of reducing agents such as ascorbic acid and cysteine. The physical and chemical properties of the fermented product were evaluated, and the effect of the reducing agents were compared between ascorbic acid and cysteine. The fermented product with the addition of ascorbic acid shows the lower pH and the higher titratable acidity comparing the product with the addition of cysteine. This indicated that ascorbic acid was more appropriate reducing agent than cysteine for the fermentation of the saccharified rice solution. The number of viable Bifidobacterium in the fermented product with the addition of ascorbic acid(2.2
108CFU/ml) was greater than that with the addition of cysteine (8
108CFU/ml). Ascorbic acid supplement also contributed better sensory properties, such as flavor, taste and overall acceptibility than cysteine supplement did.
Flavor Characteristics of Korean Traditional Distilled Liquors Produced by the Co-culture of Saccharomyces and Hansenula
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 236~245
Andong Soju is a Korean traditional distilled liquor brewed with Nuruk which is cultured with wild microorganisms. To provide useful information for scientific production and systematic quality control of traditional distilled liquor, the effects of mixed culture of the alcoholic yeasts and saccharifying molds isolated from the Nuruk, and mashes on the flavor and sensory characteristics were investigated. Distillate from mashes cocultured with Saccharomyces cerevisiae and Hansenula anomala using Mucor Nuruk was compared with distillate from mashes brewed with Andong Nuruk and with distillate from plant fermented mashes to analyze their flavor characteristics. The volatile flavor compounds in distillates were analyzed by GC and GC-MS using direct injection, solvent extraction, and purge & trap methods. Alcohols such as 3-methyl-1-butanol, 2-methyl-1-propanol, 1-propanol, and 2-phenyl ethanol; aldehydes such as acetaldehyde and 2-furancar-boxaldehyde; esters such as ethyl ester of acetic acid, hexanoic acid, octanoic acid, decanoic acid; alkanes, alkenes, ketone, sulfur, and pyrone compounds were detected. Alcohols were chief components of flavor compounds. No significant difference in overall acceptability test was shown among three experimental groups(p<0.05), but Nuruk-like aroma, Kaoliangchiew-like aroma, sweet taste, and well rounded mouthfeel showed significant differences among them(p<0.05).
Fermentation Method of Kimchi Using Halophilic Lactobacillus sp. HL-48 and Lactic Acid
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 246~251
To extend the storage period and to inhibit contamination of Kimchi by Escherichia coli, conditions of Kimchi brining and effects of the fermentation starter, halophilic Lactobacillus HL-48 were investigated. Optimum brining condition for Kimchi was accomplished in 15% NaCl and at pH2.5-3.0 adjusted by lactic acid. Starter-treated Kimchi showed pH 4.2 after 18hr fermentation, while the pH of starter-untreated Kimchi resulted in 3.3. After 36hr fermentation, the number of E. coli in starter-treated Kimchi was found clearly to decrease and not detected macroscopically, but contamination of E. coli (5.3
103CFU/ml) was observed in starter-untreated sample. Organic acids in Kimchi contained organic acids such as oxalic acid, citric acid, malic acid and lactic acid. among ther, lactic acid content was remarkably high in the early fermentation stages. However, from 24hr fermentation, lactic acid content of starter-untreated Kimchi was higher than that of starter-treated Kimchi.
Relationship between the Organic Content, Heavy Metal Concentration and Anaerobic Respiration Bacteria in the Sediments of Shiwha-ho
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 252~259
Anoxic sediments collected from Shiwha-ho area were used to find the relationship between the heavy-metal, organic content and anaerobic respiration bacteria by most probable number (MPN) method. Analysis of the sediments showed that COD content was higher in the sediments collected from Ansan-cheon and Shiwha-ho than those collected from sea area nearby. Particularly noticeable was the fact that heavy metal concentration was much higher in the sediments of Shiwha-ho area contaminated by heavy-metal, although they were rich in electron donor and electron acceptor for Fe(III)-reducing bacteria using lactate as an electron donor was in the range of 1.1
107MPNs/ml in the sediments collected from the sea-side of the lake, which were lower in heavy-methal concentration and higher in Fe-Mn content than those from other region. The number of Fe(III)-reducing bacteria using acetate as an electron donor was in the rang eof 4.3
105MPNs/ml in the same sediments. Chromate-reducing bacteria were more populated(4.6
105MPNs/ml) in the sediments contaminated by heavy metals. The number of sulfate-reducing bacteria wee counted in the sediments collected from the more contaminate inner-side than those from the sea-side of the lake.
Induction of Lactococcal /beta-Galactosidase in E. coli
Microbiology and Biotechnology Letters, volume 27, issue 3, 1999, Pages 260~265
-galactosidase gene (lacZ) from Lactococcus lactis ssp. lactis 7962 was cloned into plamid vector pKF18, which was designated as pKF-gal. Expression of the lacZ from L. lactis 7962 was found to be higher when cells were grown at 3
-galactosidase activity was obtained when E. coli/pKF-gal was cultivated for 6hr at 3
and for 3hr at 37
, and L. lactis 7962 was grown for 8hr at 3
. Enzyme induction was achieved by the addition of lactose, galactose, or lactose+IPTG to growing culture. The addition of glucose had no effect on enzyme induction.