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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 28, Issue 6 - Dec 2000
Volume 28, Issue 5 - Oct 2000
Volume 28, Issue 4 - Aug 2000
Volume 28, Issue 3 - Jun 2000
Volume 28, Issue 2 - Apr 2000
Volume 28, Issue 1 - Feb 2000
Selecting the target year
Prevalence of Antibiotic-Resistant Strains among Bacteria Isolated from Bovine Mastitis, Swine Diarrhea, and Swine Pneumonia.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 189~194
Microorganisms causing bovine mastitis, swine diarrhea and swine pneumonia were isolated from farms in Chungbuk and Kyunggi Province and the isolates were identified using microbial identification system(MIS). The most common isolates from bovine mastitis were Staphylococcus sp.,Streptococcus sp., and Corynebacterium sp., those from swine diarrhea were Escherichia coli, Salmonella sp., and Clostridium perfringens, those from swine pneumonia were Bordetella bronchiseptica and Pasteurella multocida. Examination of 16 antibiotics against these pathogens revealed that the incidence of antibiotic-resistant microoganisms were very high and that many of these isolates had multiple resistance to various commercially available antibiotics such as penicillin, ampicillin, erythromycin, streptomycin, norfloxacin, and tetracycline. These results suggest that the use of antibiotics in a farm should be controlled in order to decrease the number of antibiotic-resistant microorganisms were very high and that many of these isolates had multiple resistance to various commercially available antibiotics such as penicillin, ampicillin, erythromycin, streptomycin, norfloxacin, and tetracycline. These results suggest that the use of antibiotics in a farm should be controlled in order to decrease the number of antibiotic-resistant microorganisms.
Improvement of Bacterial Endo-1,4-,\beta-D-glucanase(CMCase) Secretion in Yeast by Mutagenesis of Glucoamylase Signal Sequence.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 195~201
Glucoamylase of Saccharomyces diastaticus is produced as a large precursor composed of signal peptide (21 amino acid residues), Thr and Ser-rich region and functional glucoamylase. To evaluate the utility of the glucoamylase signal peptide (GSP) for the secretion of foreign proteins, four types of GSP mutants (ml : Pro-18 longrightarrowLeu-18, m2 : Tyr-13 longrightarrowLeu, m3 : Ser-9longrightarrowLeu-9, m4 : Asn-5 longrightarrowPro-5) were constructed and secretion efficiency of each mutant was compared with that of native GSP by the expression and secretion of Bacillus subtilis CMCase under the control of GAP in N-terminal domain and hydrophobic domain. n mutant 4, a polar amino acid was replaced by a helix - breaking Pro residue. CMCase activity assay and Western blot analysis revealed that CMCase secretion by GSP mutants replaced by Leu were increased compared with native GSP. In the case of m2 and m3, the substitution of Leu for Tyr-13 and Ser-9 in the hydrophobic region resulted in a twofold increase in the extracellular CMCase activity.
Isolation and Characterization of Stenotrophomonas maltophilia Strains Capable of Degrading Aniline.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 202~208
Four bacteria capable of using aniline as a sole source of carbon and energy we4e isolated from river waters. Among them, two strains were identified as Stenotrophomonas maltophilia based on their physiological and biochemical characteristics and 16SrRNA gene sequence and the others as delftia acidovorans. The four strains were able to grow on the mineral salt media containing aniline at concentrations up to 6,000
/ml. Since aniline degradation by S. maltophilia has not been reported so far, the two strains A-s and 51-4 were selected for further studies. They completely utilized aniline in a mineral salt medium containing 300
/ml of aniline as a sole carbon and energy source within 24 hours. Optimum pH and temperature for aniline degradation and cell growth of both strains were 7.0 and
, respectively. In addition, they effectively degraded aniline is waste, underground and river waters containing 300
/ml of aniline. This is the first report of aniline degradation by S. maltophilia strains.
Effect of Ganoderma lucidum Mycelial Fractions on the Functional Recovery of Primary Cultured Hepatocytes.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 209~213
The cultured mycelia of Ganoderma lucidum were extracted and the extract was separated into six fractions by organic solvent fractionation. The antihepatotoxic activity of all the fractions was evaluated by measuring activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). Among the fractions tested, the high-polarity fractions such as aqueous and n-butanol fractions significantly reduced activities of GPT and GOT in CCl4- and galactosamine-intoxicated rat primary hepatocytes. When intracellular synthetic activities were measured by pulsing the rate primary cultured hepatocytes with [3H]-uridine and [3H]-leucine, activities of DNA, RNA and protein. When direct toxicities of the fractions were measured against human hepatoma(SK-Hep-1), the non-polarity fractions such as n-butanol and ethyl acetate fractions showed potent direct cytotoxicities even at the concentration of 1
/ml. These data showed that Ganoderma lucidum has hepatoprotective and hepatotoxic recovery principles in its mycelia.
Purification and Properties of an Inulinase Produced by Arthrobacter sp.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 214~218
The inulinase producing microorganism was isolated from soil and tentatively identified as Arthrobacter protophormiae/ramosus. Inulinase was pruified by ethanol precipitation, DEAE-Sephadex ion exchange chromatography and Sephadex gel filtration chromatography. The molecular weight of the purified enzyme was 34 kDa. The specific activity, yield and purity were 31.5 Unit/mg, 19.5% and 18.5 fold, respectively. Optimal pH and temperature for reaction of the purified inulinase were 8.5 and
, respectively. The enzyme was stable at pH 7.5, below
, and the activity was stimulated Mg2+.
Isolation of Alkalophilic Bacillus sp. KJ-133 Producing Cyclomaltodextrinase and Its Enzyme Production
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 219~222
To produce and utilize microbial cyclomaltodextrinase being industrially useful, we isolated an alkalophilic Bacillus strain from soil which was capable of degrading cyclodextrins. The newly isolated strain was aerobic, gram-positive, spore-forming, motile, rod shape(0.2~0.4
), and 35.8 mol% of DNA base composition. Based on its morphological, phisiological, and biochemical properties, it was identified as alkalophilic Bacillus sp. KJ-133 and cultivated well in the ranges of
and pH 8.0~9.0 . The cyclomaltodextrinase of the strain showed maximal production after 48h of cultivation at
, and the activity was inhibited by Ag2+, Hg2+, Cu2+, and p-chloromercuribenzoate.
Enantioselective Hydrolysis of (R,S)-Naproxen Methyl Ester Using Two-step Acetone-treated Candida rugosa Lipase
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 223~227
A novel two-step acetone treatment method was developed to enhance the enantioselectivity of Candida rugosa lipase (CRL) toward the hydrolysis of racemic naproxen methyl ester. The acetone-teated CRL was considerably more enantioselective than the crude CRL, yielding an enantiomeric excess of 98~100%. The crude and acetone-treated CRLs were subjected to anion exchange chromatography, and their chromatography profiles were compared. In consequence, both chromatography profiles were found to be almost identical, resulting in two separate lipase peaks (lipase A and B). The lipase B, which is known to be less enantioselective, was treated with acetone using a two-step treatment method. The enantioselectivity of acetone-treated lipase B was dramatically increased, yielding an enantiomeric excess of 99%.
Hypolipidemic Effect of Extracts of Syobean Paste Containing Mycelia of Mushrooms in Hyperlipidemic Rats.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 228~232
The hypolipidemic effects of soybeam paste containing mycelia of Phellinus linteus, Cordyceps militaris, Ganoderma lucidum were evaluated in hyperlipidemic rats. Body weight of the rats fed with soybean paste containing mycelia of Ganoderma lucidum (ESG) were significantly lower than control group whereas, there was no significant difference in body weight in order diet groups. Food intake, food efficiency ratio, and liver and kidney weights were not significantly different among the diet groups examined. However, the spleen weight of ESG group was markedly lower than other groups. Significant decreases in serum total cholesterol, LDL-cholesterol and liver total cholesterol were also observed in all diet groups tested. All groups fed with soybean paste containing mycelia of mushroom were also observed in all diet groups tested. All groups fed with soybean paste containing mycelia of mushroom were almost equally effective in increasing plasma HDL level and decreasing the atherogenic index, whereas the ratio of HDL to total cholesterol was significantly increased in all experimental diet groups.
Characteristics of Bioflocculant Produced by Achromobacter sp. YJ-66.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 233~238
To develop biodegradable and safe flocculant, Achromobacter sp. HY-66 were isolated from soil. The purified flocculant was composed of fructose and glucose in an approximate molar ratio of 1:24. Molecular weight was approximately 9
105 dalton by Sepharose CL-4B gel filtration chromatography. Elemental analysis revealed that the contents of carbon, hydrogen and oxygen were 37.72%, 5.37% and 56.89%, respectively. The presence of O-H stretching, C-H streching, carboxy ester and C-H bending were confirmed by IR absorption spectrum analysis. We suggest that a bioflocculant produced from Achromobacter sp. YJ-66 is new and different from other bioflocculants.
Distribution of Oligotrophic Actinomycetes in Forest Soil.
Microbiology and Biotechnology Letters, volume 28, issue 4, 2000, Pages 239~241
From 5 forest soil samples, the isolation frequency of oligotrophic Actinomycetes was investigated with nutrient and minimal media. The frequency of soil Actinomycetes by the minimal media. The frequency of soil Actinomycetes by the minimal media of DHV, WA, BA and NA was similar to the value by HV nutrient medium. In addition, different Actinomycetes were isolated from all the media used in this experiment and then their growth were tested on nutrient (Bennett's agar) and minimal media(WA). In consequent, the number of different Actinomycetes from minimal media was 26.1 strains, whereas the number of from nutrient medium (HV) was 26.6 strains. Furthermore, the percentage of facultative and obligate strains among the oligotrophic Actinomycetes was 90% and 10%, respectively.