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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 28, Issue 6 - Dec 2000
Volume 28, Issue 5 - Oct 2000
Volume 28, Issue 4 - Aug 2000
Volume 28, Issue 3 - Jun 2000
Volume 28, Issue 2 - Apr 2000
Volume 28, Issue 1 - Feb 2000
Selecting the target year
Isolation and Indentification of Yeast Strains Producing High Concentration of Ethanol with High Viabi-lity.
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 309~315
To isolate yeast strains producing high concentration of ethanol, 125 strains were subjected to screening. Initially 14 strains able to grow in a medium containing 15%(v/v) ethanol, 7 strains capable of growing in a medium containing 50%(v/v) glucose, 23 strains having relatively fast fermentation rates, 13 strains able to grow at
were selected. After secondary screening, 11 strains having relatively high ini-tial fermentation rate and producing high concentration of ethanol were selected. After tertiary screening 5 strains producing high concentration of ethanol were selected. These 5 strains were again for their ethanol produc-tion, residual sugar, and viability using fermentation medium containing 25% glucose. The strain producing the highest concentration of ethanol was 20-1 strain which produced 10.56%(v/v) ethanol in 4 days, and the highest viable strain was 11-1 which produced 10.35%(v/v) ethanol(13.1%. v/v) with the viability of 30.44% after 5 days of fermentation. Both of the 20-1 and 11-1 strains were identified as Saccharomyces cerevisiae.
Prevalence of Salmonella Enterotoxin Gene(stn) among Clinical Strains Isolated in Korea and Regulation of stn Expression
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 316~321
Role of enterotoxin from Salmonella in pathogenesis is not know. Enterotoxin gene from Salmonella typhimurium(stn) encodes a 29kDa toxin that has no homology to any other known enterotoxins. Expression of stn is enthanced upon contact with epithelial cell but not all strains having the stn gene express Stn, Based on PCR analysis, we found that all 36 clinical strains of Salmonella isolated in Korea tested carried the stn gene. To understand the trgulation of the stn transcription, the expression of stn was studies in vitro. RNA polymerase was purified by polymin P fraction-ation, DNA-agarose affinity chromatography, and Mono-Q ion exchange chromatography from Salmonella. The expression of stn was inhibited by cAMP·CRP complex by about 50%.
Production and Purification of Acetylcholinesterase Inhibitor from Pseudomonas sp960903
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 322~328
To screen agent for the treat-ment of Alzhimers Disease several strains of bacteria producing acetylcholinesterase inhibitor ware isolated from soil. Strain 960903 showed strong acetylcholinesteras inhibitory activity and low butyrylcholinesterse inhibitory activity. The strain 960903 was identified as Pseudomonas sp. Acetylcholinesterase inhibitor ws highly achieved in fermentation medium containing soluble starch 3.0%, glycerol 1.0%, pharmamedia 0.5%, KCI 0.3%,
for 4 days. Acetylcholinesterase inhibitor was purified by Diaion WA-30(
) column charomatography and cellulose column chromatography. Acetylcholinesterase inhibi-tor showd the maximum wavelength at 205 nm and was soluble in water, acetic acid, ethanol, methanol and dime-thyl sulfoxide. The concentration of 50% inhibition(
) of inhibitor against acetylcholinesterase was 25
/ml. The inhibitor was inactivated on heating ar
fro 15 min and more stable in acidic region than alkaline region.n.
Characterization of Extracellular Protease of Bacillus sp. WRD-1 Isolated from Soil
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 329~333
Alkaline bacterium producing a high pro-tease activity at low temperature was isolated by using enrichment culture from soil samples and identified as Bacil-lus sp. WRD-1 Cell growth was maximal at 10 hours and the optimal initial pH and culture time of culture condition for enzyme production was pH 7 and 10 hours, respectively. Temperature range of high enzyme activity were
. The optimal pH and temperature for the enzyme activity were pH9 and
Selection and Antifungal Activity of Antagonistic Bacterium Pseudomonas sp. 2112 against Red-Pepper Rotting Phytophthora capsici
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 334~340
In order to select multifunctional powerful antagonistic biocontrol agent against red-pepper rotting fungi Phytophthora capsici, we isolated an indigenous antagonistic bacterium which produces antifungal substances and siderophores from a local soil of Kyongju, Korea. The isolated strain was identified as Pseudomonas fluorescens biotype F. The antibiotic produced from P. fluorescens 2112 inhibited hyphae growth and the zoospore germination of Phytophthora capsici. The favorable carbon, nitrogen source and salts for the production of antibiotic from P. fluorescens 2112 were glycerol, beef extract and LiCi at 1.0%, 0.5% and 5 mM, respectively. And antagonistic activity of P. fluorescens 2112 was confirmed against P. capsici in vivo.
Enhanced Production of Antifungal Substance(PAFS) Bioxynthesized by Pseudomonas aeruginosa and Examination of Its Physiological Characteristics in Fermentation
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 341~348
Selection of high producer strain, optimization of production medium and cultivation in bioreactor system were carried out in order to produce an antifungal substance, PAFS in large amounts which sources and 41 kinds of nitrogen sources, a synthetic medium consisting of fructose(70 g/1) and ammonium sulfate (10g/l) and a complex medium including galactose(30g/l), fructose(20g/l) and cottonseed flour(35g/l) were determined as opti-mized media for PAFS production. In bioreactor studies examining physiological characteristics of the pro- ducer microorganism with the complex medium, typical pattern of diauxic growth was observed as demonstrated by the result that fructose was not used before almost exhaustion on readily utilizable carbon source, galactose. When galactose was supplemented additionally during the fermentation period. PAFS pro-ductivity did no increases any more, indicating that large portion of the added galactose was used for cell growth instead of biosynthesis of the secondary metabolite. It was deduced that PAFS production could be enhananced by employing fed-batch operation in order to overcome the apparent phenomenon of catabolite repression and /or inhibition.
Optmization of Culture Conditions and Nitrogen Sources for Production of Erythritol by Candida magnoliae.
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 349~354
Culture conditions and nitrogen sources were optimized for production of erythritol, a natural sweetener, by Candida magnoliae M26. The optimal culture conditions were found to be culture temperature of
, initial pH of 7, aeration of 1 vvm and agitation speed of 500 rpm in a 2.5 1 jar-fermentor. Glucose was chosen as the best carbon cource bsed on cell growth and erythritol productivity. Kight steep water(LSW) and corn steep liquor (CSL) which are by-products in starch processing from corn were tested as a nitrogen source substitute for yeast extract. The use of either LSW or CSL did not change the fermentation performance. The experimental results using LSW and CSL showed 1.5 times higher in cell growth and almost the same value in erythritol productivity com-pared with the control fermentation using yeast extract as a nitrogen source. These results suggested that either LSW of CSL could be used as a nitrogen source in a large-scale fermentation for erythritol production.
Optimization of D-\beta-hydroxybutyric Acid Fermentation Using a Mutant of Candida Rugosa IFO0750
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 355~360
A UVmutant of Candida rugosa IF00750 was made and used to convert butYlic acid to D-
-HBA). Major regulating factors for D-
-HBA fennentation were investigated via chemostat analyses. The maximum specific productivity was achieved at a specific growth rate of
where the glucose and butyric acid concentrations in the fermentor were 10 g/L and 8.7 g/L. respectively. A fed-batch fennentation was performed with maintenance of the optimum glucose and butyric acid concentrations. The D-
-HBA concentration after 120 h of cultivation reached 12.4 g/L, which was 4.7 times greater illan the concentration obtained by batch fermentation.
Protozoa as an Indicator of Activated In Sludge Plant Effluent Quality
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 361~366
pilot plant used for the treatment of municipal sewage and pre-treated night soil-containing sewage. In both case, the predominant protozoa was ciliates and among them Vorticella was the most common. In the pilot plant where pre-treated night soil was mixed with municipal sewage, genera of free-swimming ciliates, flagellates and amoeba was higher than in those withour night soil. Correlation analysis on the quality of effluent and protozoa indicates that municipal sewage has positive correlation with protozoa. However in the pilot plan 샐 sewage contatinin pre-treated night coil soil more samples show negative correlation. Followed equations were derived by the regression analysis of BOD in both the pilot plants. In case of pilot plant A of municipal sewage, the analysis B of munici-pal and pre-treated night soil-containing sewage, the analysis of BOD was $6.731
=0.864). At low temperature, number of protozoa was decreased to 35% and among therm, Aspidisca was the most common genus. Therefore, protozoa can be used as indicator of quality of the effluent in sewage treatment plants
Manufacture and Physiological Functionality of Korean Traditional Liquor by using Dandelion (Tarax-acum platycarpum)
Microbiology and Biotechnology Letters, volume 28, issue 6, 2000, Pages 367~371
In order to develop a Korean traditional liquor using dandelion, alcohol fermentation condition was investigated. Etha-nol was produced maximally when 10% dandelion and 5% nuruk were added in cooked rice and fermented by S. cerevisiae at
for 10 days. The taste and nutraceutical properties of dandelion liquor with different concentra-tions(1%, 5%, 10%) of dandelion petal were compared. The dandelion-petal liquors which was brewed by addition of 1% dandelion petals into mash showed the best acceptability and its angiotensin-converting enzyme inhibitory activity was also high.