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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 32, Issue 4 - Dec 2004
Volume 32, Issue 3 - Sep 2004
Volume 32, Issue 2 - Jun 2004
Volume 32, Issue 1 - Mar 2004
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Isolation of Immuno-stimulating Strain Bacillus pumilus JB-1 from Chungkook-jang and Fermentational Characteristics of JB-1
Kim, Yeong-Suk ; Gwon, Jeong-Suk ; Gwon, Gi-Seok ; Son, Ho-Yong ; Gwon, Ha-Yeong ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 291~296
To produce functional and high nutritional chungkook-jang, an immuno-stimulating and rapid growing bacterium was isolated from one of Korean traditional food, chungkook-jang. The isolated bacterium was identified as Bacilpumilus and deposited in Korean Collection for Type Culture (KCTC 10461BP). The chungkook-jang fermented by JB-l has good taste and pleasant smell with high content of free amino acids, and the water extract of chungkook-jang showed 410% of immuno-stimulation activity at concentration of 2 mg/ml. The rapid fermentation in 16 h is achieved by inoculation of 20 ml culture broth of JB-l into 6 kg cooked soybean in commercial scale at
Molecular Biological Studies on the Stress Protein HSP90
Gene from Flounder (Paralichthys olivaceus)
Lee, Jae-Hyeong ; Kim, Yeong-Tae ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 297~306
Heat shock proteins (HSPs) were induced in cells in the thermal stress, and the HSP90 family is one of the major classes of HSPs. Gene encoding HSPs have been characterized from various mammals and piscine. We have cloned and sequenced the HSP90 cDNA from a brain cDNA library constructed from flounder (Paralichthys oliThe result of sequence analysis shows it to be the HSP90~. The nucleotide sequence of the HSP90
was composed of 2791 long, encoding 726 amino acid residues. The flounder hsp90
gene showed very high sequence homology with hsp90f3 of European sea bass (96.6%), zebrafish (92.9%), Atlantic salmon (92.0%) and human (89.5%). We also constructed a phylogenetic tree based on HSP90 amino acid sequences from vertebrate species. Gene-specific primers were selected and used in RT-PCR reactions to measure the basal hsp90
mRNA. The hsp90f3 gene is constitutively expressed at a fairly high level in all examined tissues (brain, liver, kidney, muscle, and spleen). In order to express protein of flounder hsp90
in E. coli, we used the His-tagged pETvector. Then, the expression of flounder HSP90
was confirmed by Western blot analysis.
Characterization of an Antioxidant from Sporophyll of Undaria pinnatifida
Yang, Ji-Yeong ; Yu, Mi-Yeong ; Kim, Sang-Gwon ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 307~311
Hot water soluble extract from sporophyll of Undaria pinnatifida was examined for antioxidant activity using l,l-diphenyl-picrylhydrazyl (DPPH) and lipid peroxidation assay. The
value (7.6 mg/ml) of hot water extract from sporophyll of U. pinnatifida was more higher than ascorbic acid (0.025 mg/ml) and BHT (0.25 mg/ml). The hot water extract from sporoof U. pinnatifida was stable from pH 2 to pH 7 but decreased at alkali pH. The heat stability of hot water extract from sporophyll of U. pinnatifida was stable from
. An effect on an antioxidant activity of hot water extract from sporophyll of U. pinnatifida was studied with various metal ions and EDTA. Antioxiactvity of hot water extract from sporophyll of U. pinnatifida was inhibited by EDTA but increased by adding
Selection and Antagonistic Mechanism of Pseudomonas fluorescens 4059 Against Phytophthora Blight Disease
Jeong, Hui-Gyeong ; Kim, Sang-Dal ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 312~316
In oder to select the powerful rhizophere-dorminatable biocontrol agent, we had isolated an indigenous antagonistic bacterium which produced antibiotic and siderophore from a disease suppressive local field soil of Gyungsan, Korea. And we could select the Pseudomosp. 4059 which can strongly antagonize against Fusarium oxysporum and Phytophthora capsici by two kinds of antifungal mechanism that can be caused by the antibiotic of Phenazin, a siderophore and a auxin like subThe selected strain was identified as Pseudomonas fluorescens (biotype A) 4059 by biochemical tests, API
test, MicroLog TM system and 16S rDNA analysis. The selected antagonistic microorganism, Pseudomosp. 4059 had an antifungal mechanism of antifungal antibiotic and sidrophore. And we were confirmed the antagonistic activity of P fluorescens 4059 with in vitro antifungal test against Phytophthora capsici and in vivo by red-pepper.
Optimization of Extraction Conditions of Antioxidants from Sporophyll of Undaria pinnatifida by Response Surface Methodology
Yu, Mi-Yeong ; Kim, Gyeong-Hwan ; Lee, Jae-U ; Kim, Sang-Gwon ; Yang, Ji-Yeong ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 317~321
We selected a solvent for producing antioxidants from sporophyll of Undaria pinnatOptimum extraction condition for antioxidant ability(Y) of sporophyll of U. pinnatifida was investigated using response surface methodology(RSM). A compound central design was used with variables (
) (3.4; 20; 60; 100; 116.6); (
) pH(1.8; 3; 6; 9; 10.2) and (
) treatment time(min) (1.7; 10; 30; 50; 58.3). Antioxidant activities were increased with decreasing pH and increasing temperature. The optimal extraction conditions for antioxidant ability(Y) of sporophyll of U. pinnatifida were found to be temperature
, pH 4.2 and treatment time 28.2 min. Among the variables tested, pH showed greater significant facin extraction of antioxidants from sporophyll of U. pinnatifida.
Process Optimization for Concentration and Stabilization of Recombinant Endoxylanase Expressed in Bacillus subtilis
Choe, Yeong-Rok ; Park, Jeong-Ha ; ; Kim, Yeong-Man ; Gwon, Hyeon-Ju ; Kim, Byeong-U ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 322~327
A strong constitutive PJH promoter from Bacillus sp. was applied to overexpress the endoxylanase gene (639 bp) in Bacillus subtilis. The expression plasmid, pJHKJ4, was designed to contain the
promoter and open reading frame of endoxylanase including its own promoter. The plasmid was introduced into B. subtilis DB431 and the resulting transformant was grown on LB glucose medium. At the end of cultivation, the endoxylanase activity in the culture supernatant reached about 140 DIm!. The enzyme in the supernatant was concentrated by ultrafiltration (MW cut-off 10 kDa and 30 kDa) and ammonium sulfate precipitation. For the concentration of the enzyme, ultrafiltration was more efficient than 70% ammonium sulfate precipitation. The stabilization of concentrated enzyme solution at
was examined with various stabilizers such as NaCI, glycerol, polyethylene glycol, sorbitol, and
. The most effective stabilizers were found to be NaCI and
Removal of High Strength Hydrogen Sulfide Gas using a Bioreactor Immobilized with Acidithiobacillus ferrooxidans and a Chemical Absorption Scrubber
Ryu, Hui-Uk ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 328~333
To treat a waste gas containing a high strength H2S, the two-stages microbial desulfurization process that conof a bioreactor immobilized with Acidithiobacillusferrooxidans and a chemical absorption scrubber has was proposed. After 4 times repeat of batch cultures, the immobilized bioreactor has been stabilized and the rate of iron oxidation reached 0.89 kg .
at steady state. The two-stages microbial desulfurization prowas able to be operated for a long term over 54 days. The removal efficiencies of H2S were 97-99% at a space velocity of 70 h-I and a inlet concentration of 37,000 ppmv. The maximum elimination capacity of H2S was approximately 3.3 kg S .
. In the bioractor, the concentrations of the
and the immobilzed cell were constantly maintained during the desulfurization.
An Ecological Analysis of Lichens Distributed in Rocks of Coast and Field in U-do by Molecular Technique
Gang, Hyeong-Il ; Yun, Byeong-Jun ; Kim, Seong-Hyeon ; Sin, Deok-Ja ; Kim, Hyeon-U ; Heo, Jae-Seon ; Gang, Ui-Seong ; O, Gye-Heon ; Go, Yeong-Jin ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 334~340
This study was initially performed to obtain fundamental data on lichen distribution depending on environmental condiLichens distributed on coastal rocks atleast 20 m far away from the coast were also investigated. Analyof morphological characteristics and rDNA ITS clones revealed that lichens containing 9 families and 15 genera were distributed in coastal rocks of V-do, while lichens containing 10 families and 14 genera were in field rocks, demonstrating little difference of diversity between both sites. Foliose lichens such as PhaeophysPhyscia, Pyxine belonging to the family Physciaceae were representatives distributed in coastal rocks of V-do, along with Xanthoparmelia belonging to Parmeliaceae, fruticose lichens such as Ramalina to Ramaliand crustose lichen Lecanora to Lecanoraceae. In contrast, foliose lichen Cladonia belonging to the family Cladoniaceae, fruitcose lichen Stereocaulon to Streocaulaceae, and crustose lichen Porpidia to Porpidwere found only on the surface of field rocks. Crustose lichens containing genera Caloplaca, CanDirinaria, Graphis, Rhizocarpon, and Pertusaria were rarely distributed in coastal rocks, and most of them were also found in field rocks.
Quantification of Inhibitory Impact of Heavy Metals on the Growth of Escherichia coli
Jo, Gyeong-Suk ; Gu, So-Yeon ; Kim, Ji-Yeong ; Ryu, Hui-Uk ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 341~346
The quantification of the toxicological effects of the heavy metals such as Cu, Cd, Cr, Hg, and Zn on the growth of Escherichia coli was performed, and the variations of toxicwith exposure time were evaluated in adaptation procedure. The characteristics of growth inhibition on Escherichia coli by heavy metals were different with metal species, and critical concentration of each metal, which inhibited cell growth completely, were Cu of 3.5 mM, Zn of 2.5 mM, Cd of 1.5 mM, Cr of 1.2 mM, and Hg of 0.12 mM, respectively. The tolerance of E. coli against heavy metals, based on
values, increased in order of Cu > Zn > Cr > Cd > Hg. The slopes obtained from the relationship between ECso values and expose time corresponds to adaptability of test organisms to the toxicants. The adaptability of test organisms to the toxicants was much higher at higher slope values. Adaptability of E. coli on heavy metals increased in order to Zn > Cd > CU >> Cr> Hg.
Hydrolysis of Galactomannan and Manno-oligosaccharides by A Bacillus subtiis Mannanase
Gwon, Min-A ; Yun, Gi-Hong ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 347~351
Hydrolysis of manno-oligosaccharides and galactomannan was studied with the purified Bacillus subtilis WL-7 mannanase from recombinant Eschericoli. The predominant products of hydrolysis were mannose, mannobiose and mannotriose. The enzyme could hydrolyze
-1 A-linked manno-oligosaccharides larger than mannobiose, but was not active on mannobiose. When the mannanase hydrolyzed manno-oligo saccharides of degree of polymerization(DP) 4-6, it was more active on the substrate of higher DP. Based on analysis of transient reaction products by TLC, the enzyme was found to have a preference for internal
-IA-mannosidic linkages, which are the central mannosidic bond of mannotetraose and the two middle mannosidic bonds of mannopentaose. The
-l A-mannosidic bonds situated at the second and fourth positions from the nonreducing end of mannohexaose were preferenhydrolyzed by the mannanase. Locust bean gum(LBG) was enzymatically hydrolyzed with higher efficiency than guar gum, resulting that amount of reducing sugars was liberated more efficiently from LBG than guar gum with same activity of mannanase.
Screening of Antimicrobial Substances against Brachyspira hyodysenteriae
Kim, Jin-Gyu ; O, Se-Taek ; Yang, Si-Yong ; Kim, Seon-Yeo ; Gang, Hyeon-Mi ; Mun, Jin-San ; Song, Min-Dong ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 352~355
The extracts from 70 different plant natural products were used to investigate the antimicrobial activities against Brachyspira hyodrsenteriae, the causative agent of swine dysentery. Among them, the extracts from Terminalia chehula exhibited the highest antimicrobial activities against B. hyodysenteriae. It showed the antimicrobial activity against B. hyodysensteriae mostly in parts of its shell. Minimum inhibitory concentration (MIC) of the water extracts of T chehula was 12.5 mg/ml. After organic solvent extractions, the ethylacetate extracts exhibited higher antimicrobial activities against B. hyodysenteriae, and their antimicroactivities were similar to that of the tannin. In conclusion, these findings suggest that the extracts from T chebula may be used as animal feed additives for their antimicrobial activities against B. hyodysenteriae.
Expression of Ethionine Resistance Conferring Gene in an Industrial Strain of Saccharomyces cerevisiae
Park, Jeong-Nam ; Lee, Gyeong-Hui ; Go, Hyeon-Mi ; Seo, Guk-Heon ; Jin, Jong-Eon ; Lee, Hwang-Hui ; Bae, Seok ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 356~361
The ethionine resisconferring gene (ERCI) was constitutively expressed under the control of the alcohol dehydrogenase gene promoter (ADClp) and introduced into the chromosomes of an industrial polyploid strain of Saccharocerevisiae by using the 8-sequences of the Tyl retrotransposon as the recombination site. 8-Integrative cassette devoid of bacterial DNA sequences containing the ampicillin resistance gene was constructed that had the aureobasidin A resistance gene (AURl-C) as the selection marker and ERCl gene. The ERCl gene was also employed as the selection marker in the 8-integrative cassette lacking the A URl-C gene. Industrial Saccerevisiae transformed with these integrative cassettes exhibited strong resistance to DL-ethioncompared with nontransformants.
Purification of Fucoidan from Korean Sea Tangle (Laminaria religosa) and Isolation of Fucoidan-Degrading Microorganisms
Kim, Dae-Seon ; Im, Dong-Jung ; Mun, Seong-Hun ; Seo, Hyeon-Hyo ; Park, Yong-Il ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 362~365
The fucoidan from Laminaria relicollected at Wando in Korea was purified with the yield of 2.3% in mass. The monosaccharide composiof the purified fucoidan was nearly identical to that of the commercial standard: fucose 63.71 %, xylose 22.98%, galactose 6.62%, mannose 0.24%, and uronic acid 3.26%. Microorganisms capable of degrading the purified fucoidan were isolated from the colonies on the minimal medium containing 0.2% of purified fucoidan as a sole carbon source. Of these isolates, a strain showing a relatively higher capability to degrade fucoidan, up to 63%, was partially characterized as a Gram positive, aerobic, moderately halophilic marine bacterium.
Effects of Organic Nutrients on Chitinase Production in Minimal Media
Jang, Ji-Yun ; Kim, In-Cheol ; Jang, Hae-Chun ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 366~370
Four chitinase producing bacteria, Arthrobacter nicotinae CH4, Arthrobacter nicotinae CHI3, Arthrobacter sp. CH5 and Micrococcus sp. CH3, were isolated from small crabs and shrimps. We investigated the optimum medium condition for the production of enzyme and high cell mass. The preferable medium composition was as follows: colchitin 0.1 %(w/v), glycerol 0.25%(w/v) and yeast extract 0.05%(w/v) in minimal midium (
0.001 g/l, pH 7.0). This cell culture medium could be used directly as sample for measuring chitinase activity. Because it hardly conreducing sugar such as glucose (blank value=0), the detected reducing sugar can be considered as a chitinase reaction product. The results can be used for easy preparation method for determination of enzyme activity and analysis of enzyme-substrate reaction in step of screening of chitinase producing bacteria.
Isolation of Chryseobacterium meningosepticum Producing
-Mannosidase from a Mudfish
Kim, Hyeon-Suk ; Yun, Gi-Hong ;
Microbiology and Biotechnology Letters, volume 32, issue 4, 2004, Pages 371~374
A bacterium producing the
-mannosidase was isolated from intestine of fresh fish. The isolate YB-29 has been identified as Chryseomeningosepticum on the basis on its 16S rRNA sequence, morphology and biochemical proper The
-mannosdiase activity was detected in both the culture filtrate and the cell extract of C. meningosepticum YB-29. The
-mannosidase of culture filtrate showed the maximum activity for hydrolysis of para-nitrophenyl-
-D-mannopyranoside at pH 5.0-6.0 and 55-
. The enzyme was able to hydrolyze the oligomeric substrates such as mannobiose and mannotriose with higher activity for mannotriose than mannobiose.