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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Microbiology and Biotechnology Letters
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Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 33, Issue 4 - Dec 2005
Volume 33, Issue 3 - Sep 2005
Volume 33, Issue 2 - Jun 2005
Volume 33, Issue 1 - Mar 2005
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Bioactive Substances from Ganoderma lucidum
Bae Woo-Chul ; Kim Yong-Seok ; Lee Jun-Woo ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 75~83
The popular edible mushroom Ganoderma lucidum was considered the most valuable medicine in ancient Asia and was belived to bring longevity, due to its mysterious power of healing the body and calming the mind. Today, Ganoderma lucidum is still widely revered as a valuable health supplement and herbal medicine worldwide, as studies (mostly conducted in China, Korea, Japan, America) into the medicinal and nutritional values of Ganoderma lucidum revealed that it does indeed contain certain bioactive ingredient (such as polysaccharide, triterpene) that might be benefical for the prevention and treatment of a variety of ailments, including diseases such as hypertension, diabetes, hepatitis, cancer, AIDS.
Expression and Cloning of the pmmC Gene Encoding Phosphomannomutase in Sphingomonas chungbukensis DJ77
Kim Mi-Hye ; Choi Jung-Do ; Shin Malshick ; Kim Young-Chang ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 84~89
Phosphomannomutase (PMM) is a key enzyme in prokaryotes and eukaryotes, which catalyzes the conversion of
-D-mannose 6-phosphate to
-D-mannose 1-phosphate. The latter is the substrate for the synthesis of GDP-mannose, which serves as the mannosyl donor for many metabolic pathways in the cells. We report here on the isolation of a gene from a genomic library of Sphingomonas chungbukensis DJ77, the pmmC gene encoding phosphomannomutase. The gene was cloned into E. coli expression vector, and the sequence was analyzed. The ribosomal binding site GGAAG lays 5 bp upstream of the ORF of 750 bp, which is initiated by ATG codon and terminated by TAG. The predicted sequence of the enzyme consists of 249 amino acids with a molecular mass of 27.4 kDa and showed
similarity to that of eukaryotic phosphomannomutase after bioinformatical analyses with the conserved domain search of NCBI. The purified gene product revealed the activity of phosphomannomutase. In conclusion, we confirmed that pmmC gene encodes phosphomannomutase actually.
Isolation and Identification of Streptomyces sp. Producing Anti-vancomycin Resistant Staphylococcus aureus Substance
Oh Se-Teak ; Lee Jun-Jae ; Lee Ji-Youn ; Kim Jin-Kyu ; Yang Si-Yong ; Kim Yang-Soo ; Song Min-Dong ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 90~95
An Actinomycetes producing an anti-VRSA (vancomycin-resistant Staphylococcus aureus) substance was isolated from soil. The cultural, morphological, physiological and phylogenetic analyses of an isolated strain were investigated for identification. Cultural characteristics based on ISP (International Streptomyces Project) were as follows: white aerial mycelium, yellow reverse side, and good growth on various medium. Also, the isolate did not produce the soluble pigment. Morphological characteristics were showed cylindrical spore chain and smooth spore surface by SEM (Scanning Electron Microscope). Physiological characteristics were showed LL-type by DAP isomer analysis and detected glycine, glutamic acid and alanine. A phylogenetic analysis of the 16S rDNA provided a clue that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces echinatus. The isolate was identified to be a genus of Streptomyces sp.. The optimal culture conditions for the maximum production of anti-VRSA substance by Streptomyces sp. were attained in a culture medium composed of
(w/v) glucose, and
(w/v) yeast extract. The anti-VRSA substance was highly produced after 5 days of culture. Optimal pH and temperature conditions for the production of anti-VRSA substance were pH 7.0 and
Characterization and Cloning of the Gene Encoding Autoregulator Receptor Protein from Streptomyces longwoodensis
Yeo Soo-Hwan ; Lee Sung-Bong ; Kim Hyun-Soo ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 96~105
For screening of autoregulator receptor gene from Streptomyces longwoodensis, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHIsite of pUC19 and transformed into the E. coli
. The isolated plasmid from transformant contained the fragment of 100 bp, which was detected on
gel after BamHI treatment. The insert, 100 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridizations with the 100 bp fragment as a probe allowed to select a genomic clone of S. longwoodensis, pSLT harboring a 4.4 kb SphI fragment. Nucleotide sequencing analyses revealed a 651 bp open reading frame(ORF) were isolated protein showing moderate homology (
) with the
-butyrolactone autoregulator receptors from Streptomyces sp., and this ORF was named sltR The sltR/pET-17b plasmid was constructed to overexpress the recombinant SltR protein (rSltR) in E. coli BL21 (DE3)/pLysS, and the rSltR protein was purified to homogeneity by DEAE-Sephacel column chromatography, and DEAE-5PW chromatography (HPLC). The molecular mass of the purified rSltR protein was 55 kDa by HPLC gel-filtration chromatography and 28 kDa by SDS-PAGE, indicating that the rSltR protein is present as a dimer. A binding assay with tritium-labeled autoregulators revealed that the rSltR has clear binding activity with a A-factor type autoregulator as the most effective ligand.
Substrate Variety of a Non-metal Dependent Tagatose-6-phosphate Isomerase from Staphylococcus aureus
Oh Deok-Kun ; Ji Eun-Soo ; Kwon Young-Deok ; Kim Hye-Jung ; Kim Pil ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 106~111
To investigate the substrate variety of a putative non-metal dependent isomerase, the tagatose-6-phosphate isomerase (E.C. 126.96.36.199) structural genes (lacB; 510bp and lacA; 430bp) of Staphylococcus aureus were subcloned and co-expressed. Based on the substrate configuration, various aldoses were surveyed for substrate of ketose isomerization. Among the 10 aldoses tested, D-ribose and D-allose were isomerized by the enzyme. The subunit A and B showed more than
activity for D-ribose and
for D-allose in the presence of 1mM EDTA compared with non-EDTA conditions, which implying tagatose-6-phosphate isomerase is a non-metal dependent isomerase. Each of subunit A or subunit B alone showed no activity for any of the substrates tested. The affinity constant (
) of tagatose-6-phosphate isomerase against D-ribose and D-allose were 26 mM and 142 mM, respectively.
Culture Conditions and Antifungal Activity of Bacillus licheniformis KMU-3 against Crop Pathogenic Fungi
Park Sung-Min ; Han Sun-Hee ; Yu Tae-Shick ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 112~116
Bacillus licheniformis KMU-3 shown a strong antifungal activity was isolated from Swedish forest soils. B. licheniformis KMU-3 produced a maximum level of antifungal substance under incubation aerobically at
for 24 hours in LB broth containing
ammonium sulfate at 180 rpm and initial pH adjusted to 8.0. Chloroform extraction of culture broth was confirmed inhibitory zone by plate assay and Rf value 0.49 substance by thin layer chromatography (TLC) represented high antifungal activity against Rhizoctonia solani AG-1. This substance also exhibited against Rhizoctonia solani AG-4, Colletotrichum orbiculare, Colletotrichum gloeosporioides, Cladosporium cucumerinum, Fusarium oxysporum, and Fusarium graminearum.
Optimization of Heteropolysaccharide-7 Production by Beijerinckia Indica
Wu Jian-Rong ; Son Jeong Hwa ; Kim Ki Myong ; Nam Soo-Wan ; Lee Jin-Woo ; Kim Sung-Koo ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 117~122
Beijerinckia indica was cultured in mineral salts medium (MSM) medium with various carbon and nitrogen sources to improve the production yield of heteropolysaccharide-7 (PS-7). At high C/N ratio, the high concentration of PS-7 was produced until 40 h of the culture, whereas most of the glucose as a carbon source was used for the cell growth at low C/N ratio. However, at the high C/N ratio, PS-7 accumulation stopped at 48 h of the culture due to the increasing viscosity of the culture broth would inhibit the cell growth. Therefore, the optimized value of C/N ratio was 33.3 (20 g/L glucose, 7.5 mM
) for the high production of PS-7. In the culture with various carbon sources, B. indica effectively used the hexoses or glucose-generating sugars for PS-7 formation. Especially, sucrose was the best carbon source for the high production of PS-7 (6.96 g/L) with a high viscosity (40772 cp). In the culture of B. indica with MSM medium containing 20 g/L glucose and 7.5 mM
in a 51 fermentor, the highest cell concentration was 2.5 g/L and the highest concentration of PS-7 was 7.5 g/L (35174 cp). The additional nitrogen sources of 7.5 mM
, glutamine and glutamate at 12 h of the culture after exhaustion of a nitrogen source regulated the metabolism of carbon sources, therefore the nitrogen sources could control PS-7 synthesis.
Isolation and Identification of Antimicrobial Compound from Amarantus lividus
Oh, Young-Sook ; Lee, Shin-Ho ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 123~129
Isolation and identification of pathogens from slaughter and meat processing plant were investigated. Antimicrobial activity of Amaranthus lividus against isolated pathogens such as Aeromonas sobria, Escherichia coli, Escherichia coli O157, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus was investigated. Among the chloroform, ethyl acetate and buthanol fraction of amaranthus lividus showed inhibitory effect against Aeromonas sobria CLFM1 and Escherichia coli CLFM2. Antimicrobial substance in chloroform fraction was isolated by silica gel adsorption column chromatography, sephadex LH-20 column chromatography and silica gel partition column chromatography. The antimicrobial compound of amaranthus lividus was identified as diethyl phtalate by HPLC, GC-MS, H-NMR and C-NMR.
Synergistic Antimicrobial Effect of Patrinia scabiosaefolia and Forsythiae fructus Extracts on Food-borne Pathogens
Bae Ji-Hyun ; Son Kug-Hee ; Lee Eun-Joo ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 130~135
To investigate the antimicrobial effect of the Patrinia scabiosaefolia extracts against food-borne pathogens, we extracted the P. scabiosaefolia with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The antimicrobial activity of the P. scabiosaefolia extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of P. scabiosaefolia showed the highest antimicrobial activity against Escherichia coli and Shigella sonnei. Synergistic effect in inhibition was observed when P. scabiosaefolia extract was mixed Forsythiae fructus extract as compared to each extracts alone. Finally, the growth inhibition curves were determined by using ethyl acetate extracts of P. scabiosaefolia against Staphylococcus epidermidis and Shigella sonnei. The ethyl acetate extract of P. scabiosaefolia had strong antimicrobial activity against S. sonnei at the concentration of 4,000 ppm. At this concentration, the growth of S. Sonnei was retarded more than 72 hours and up to 48 hours for S. epidermidis. These results suggest that the ethyl acetate extracts of P. scabiosaefolia can be used for the efficient material against the growth of S. epidermidis and S. sonnei.
Simultaneous Removal of Nitrogen and Phosphorus Leached from Farming Feed by the Marine Bacteria, Bacillus sp. CK-10 and Bacillus CK-13, Isolated from Shrimp Farming Pond
Chun Jae-Woo ; Ma Chae-Woo ; Kahng Hyung-Yeel ; Oh Kye-Heon ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 136~141
A bench-scale feasibility study was conducted with solid farming feed to evaluate a treatment process for microbiological removal of nitrogen (N) and phosphorus (P). Strains, Bacillus sp. CK-10 and Bacillus sp. CK-13, were originally isolated from water samples of shrimp farming pond. Simultaneous removal of N/P in marine media was monitored in the co-cultures, CK-10 and CK-13. As the results,
were eliminated within 12 hours and
within 36 hours, and
was completely disappeared within 36 hours from the media. Cultures of CK-10 and CK-13 were applied for removal of N/P leached from shrimp farming fred. HPAEC-PAD system was used to analyze sugars in farming feed, resulting in resolution of various sugars including glucose, galactose, galatosamine, mannose, and fucose.
(w/v) Pulp densities of the farming feed contained approximately
which could dissolved within 72 hours of leaching in aqueous solution followed by bacterial removal. Complete bacterial removal of N/P was achieved within 84 hours at
of the feed in co-cultures, whereas single cultures removed to incompletion of N/P during the incubation period. This work demonstrated that test cultures, CK-10 and CK-13 showed effective removal of N/P derived from shrimp farming feed.
Production of Antibacterial Substance against Bovine Pneumoniae Bacteria from Agastache rugosa
Jang Bong-Gak ; Lee Dae-Hyoung ; Lee Jong-Soo ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 142~147
The objective of this study was to develop a new potent antibacterial compound against bovine pneumoniae bacteria from medicinal plants or herbs. Among 65 kinds of medicinal plants and herbs, ethanol extracts of Citrus unishiu showed the highest solid yield of
. However, ethanal extracts from Agastache rugosa had the highest antibacterial activities against bovine pneumoniae bacteria, Mannheimia haemolytica A and Haemophilus somnus (size of clear zone: 16.0 mm and 10.0 mm, respectively). The antibacterial compound was also maximally extracted when the powder of A. rugosa was treated with
for 12 hours.
Novel Real Time PCR Method for Detection of Plasmodium vivax
Ki, Yeon-Ah ; Kim, So-Youn ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 148~153
Malaria is a re-emerging infectious disease that is spreading to areas where it had been eradicated, such as Eastern Europe and Central Asia. To avoid the mortality from malaria, early detection of the parasite is a very important issue. The peripheral blood smear has been the gold standard method for the diagnosis of malaria infection. Recently, several other methods have been introduced for quantitative detection of malaria parasites. Real time PCR that employs fluorescent labels to enable the continuous monitoring of PCR product formation throughout the reaction has recently been used to detect several human malaria parasites. 18S rRNA sequences from malaria parasites have been amplified using Taqman real time PCR assay. Here, a SYBR Green-based real time quantitative PCR assay for the detection of malaria parasite-especially, Plasmodium vivax - was applied for the evaluation of 26 blood samples from Korean malaria patients. Even though SYBR Green-based real time PCR is easier and cheaper than Taqman-based assay, SYBR Green-based assay cannot be used because 18S rRNA cannot be specifically amplified using 1 primer set. Therefore, we used DBP gene sequences from Plasmodium vivax, which is specific for the SYBR Green based assays. We amplified the DBP gene from the 26 blood samples of malaria patients using SYBR Green based assay and obtained the copy numbers of DBP genes for each sample. Also, we selected optimal reference gene between ACTB and B2M using real time assay to get the stable genes regardless of Malaria titer. Using selected ACTB reference genes, we successfully converted the copy numbers from samples into titer,
of parasites per microliter. Using the resultant titer from DBP based SYBER Green assay with ACTB reference gene, we compared the results from our study with the titer from Taqman-based assay. We found that our results showed identical tendency with the results of 18S rRNA Taqman assay, especially in lower titer range. Thus, our DBP gene-utilized real time assay can detect Plasmodium vivax in Korean patient group semi-quantitatively and easily.
Detection of cry-type Genes of Bacillus thuringiensis Isolates from Korea
Park Sooil ; Lee Kwang Yong ; Kang Eun Young ; Kim Eui Na ; Kwon Hyuk Han ; Ahn Seong Kyu ; Lee Hyung Hoan ;
Microbiology and Biotechnology Letters, volume 33, issue 2, 2005, Pages 154~158
Twenty-three Bacillus thuringiensis strains isolated from Korea were screened to detect the cry-type genes using PCR with 21 specific oligonucleotide primers. Eight strains contained distinct multiple crystal genes; cry1Aa2, cry1Ab1, cry1Ac1 and cry2Aa1. These results indicate that the strains coincided with the B. thuringiensis subsp. kurstaki strain. The other 15 strains were not recognised to the 21 specific primers.