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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 35, Issue 4 - Dec 2007
Volume 35, Issue 3 - Sep 2007
Volume 35, Issue 2 - Jun 2007
Volume 35, Issue 1 - Mar 2007
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Pomegranate (Punica granatum) as Resources of Phytoestrogen and Anticancer Substances.
Song, Bang-Ho ; Tran, Hoang Ngoc Ai ; Bae, Soo-Young ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 81~97
Punica granatum, L. (Pomegranate) has 613 seeds which accidentally corresponds to the 613 commandments in the Bible. Accordingly, the fruit has been worshipped by the Jewish and other religious people from the ancient. Pomegranate's seed, peel and juice contain a variety of ethnomedical components so much as the sum of three kinds of other common fruits. The number of published papers related to the pomegranate in recent 7 years flourished 7 times more than before at the bases of Medline record. Since the containments of estrogen, as
, estrone, and estradiol, etc., in pomegranate have been reported, public interests and commercial values of pomegranate arose considerably. The report was disproved later, however, merits of this fruit remained yet; clinical efficacy for preventing and remediating cancers including breast and prostate cancers by oral administration of the juice, seed oil, and peel extract is still believed to be true. In this review, target components of pomegranate such as antioxidants, anticancers, antiestrogens and ethnomedical components were analyzed and discussed along with examining its pharmaceutical efficacy and prescription to postmenopausal lesion, cardiosclerosis, cosmetic beautification, viral and allergic symptoms, and diabetes mellitus, etc.
Probiotic Properties of Lactobacillus salivarius CPM-7 Isolated from Chicken Feces.
Lim, Soo-Jin ; Jang, Sung-Sik ; Kang, Dae-Kyung ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 98~103
To isolate probiotic lactic acid bacteria for animal, we have screened the microorganisms from chicken feces, by random selection and agar well diffusion assay. Among them, CPM-7 strain showing superior inhibitory activity against Escherichia coli was selected. By examining carbohydrates utilization, morphologic property and 16S rRNA gene sequence, CPM-7 strain was identified as Lactobacillus salivarius, then named L. salivarius CPM-7. L. salivarius CPM-7 produced thirteen enzymes in the test using API ZYM kit, and showed resistance to low pH and bile salts. It survived at pH 2 for 30 min. and pH 3 for 6 hr. And, it was able to grow in MRS medium containing 0.2% (w/v) bile salts. L. salivarius CPM-7 adhered to the jejunal epithelium cells of pig. Both the supernatant of L. salivarius CPM-7 and the its neutralized one showed high inhibitory activity against E. coli K88.
Comparative Analysis of Aniline Dioxygenase Genes from Aniline Degrading Bacteria, Burkholderia sp. HY1 and Delftia sp. HY99.
Kahng, Hyung-Yeel ; Oh, Kye-Heon ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 104~111
In this study, aniline dioxygenase genes responsible for initial catabolism of aniline in Burkholderia sp. HY1 and Delftia sp. HY99 were cloned and the amino acid sequences were comparatively analyzed, which already have been reported as bacteria utilizing aniline as a sole source of carbon and nitrogen, B. sp. HY1 was found to have at least a plasmid, and the plasmld-cured strain, B. sp. HY1-PC obtained using mitomycin C was tested with wild type strain to investigate whether the former maintained the degradability for aniline. This proved that the aniline oxygenase gene from B. sp. HY1 was located in chromosomal DNA, not in plasmid DNA. Aniline dioxygenase small subunits from B. sp. HY1 and D. sp. HY99 were found, based on 146 amino acids, to share 79% similarity. Notably, ado2 genes from B. sp. HY1 and D. sp. HY99 which were found to be terminal dioxygenase of aniline dioxygenase small subunit showed 99% similarity in the deduced amino acid sequences with tdnA2 of Frateuria sp. ANA-18 and danA2 of D. sp. AN3, respectively. Besides, enzyme assay and amino acid sequence analysis of catechol dioxygenase supported the previous report that B. sp. HY1 might occupy ortho-cleavage pathway using catechol 1,2-dioxygenase, while D. sp. HY99 might occupy catechol 2,3-dioxygenase for meta-cleavage pathway.
Identification of Protein Kinases by Anti-phosphoserine/Phosphothreonine/Phosphotyrosine Antibody Immunoaffinity Column Chromatographies in Streptomyces griseus.
Cheong, Yong-Hoon ; Kim, Jong-Hee ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 112~117
Protein kinases play very important role for maintaining viability in prokaryote and eukaryote. The metabolism of prokaryotic cell is generally regulated by bacterial two-component regulatory systems that are composed of histidine and asparitic acid kinases, however, some eukaryotic signal transduction system such as, serine and threonine kinases, have been also found to be involved in the regulation of morphogenesis and physiological differentiation in Streptomyces. Streptomyces griseus, a streptomycin producer, was expected to have varlous types of eukaryotic-type serine/threonine protein kinases, controlling morphogenesis. Thus, many steps of chromatographies were applied to isolate serine and threonine kinases from S. griseus IFO13350. The immunoaffinity steps using anti-phosphoserine, anti-phosphothreonine, and anti-phosphotyrosine agarose column chramatographies were successfully introduced to identify eukaryotic protein kinases from S. griseus IFO13350. Eight proteins with the expected molecular weight of 14, 29, 31, 35, 40, 52, 56, and 60 kDa, were identified on SDS-PAGE, and the their kination activity was confirmed by nonradioactive protein kination assay using FITC-labeled peptide as the substrate.
Germanium-Fortified Yeast Activates Macrophage, NK Cells and B Cells and Inhibits Tumor Progression in Mice.
Baek, Dae-Heoun ; Jung, Jin-Wook ; Sohn, Tsang-Uk ; Kang, Jong-Koo ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 118~127
Germanium-fortified yeast (GY) is a organic germanium-fortified yeast with potent immune modulating activities including anti-inflammatory effect. Through cell line studies, we observed that GY can modulate the diverse immune activity but little evidence was provided on the mechanism of GY in modulating immune activities in other higher animals. In this study, we investigated the effect of GY on modulation of immune function in mice. GY was administered in normal mice or tumor-bearing mice and then effect of GY on modulation of host immune system was analyzed by using ex vivo isolated macrophages, B cells, NK cells. Admistration of GY in mice induced macrophage activation thereby increased effector function of macrophage such as increased phagocytosis, chemotaxis, adherence,
production. In addition, GY administration Increased B lymphocyte activation and plaque forming cells. Furthermore, GY administration increased NK-cell mediated cytotoxicity. Furthermore, GY administration suppressed progression of tumor in mice by increasing
production and effector function of NK cells. Our results showed that GY has a potent immunostimulatory function in vivo mice model. Proper modulation and administration of GY in human could be helpful to maintaining immunological homeostasis by modulating host immune system.
Purification and Characterization of the Siderophore from Bacillus licheniformis K11, a Multi-functional Plant Growth Promoting Rhizobacterium.
Woo, Sang-Min ; Woo, Jae-Uk ; Kim, Sang-Dal ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 128~134
Previously, we isolated plant growth promoting rhizobacterium (PGPR) Bacillus licheniformis K11 which could produce auxin, cellulase and siderophore. The siderophore of B. licheniformis K11
was determined to be a catechol type siderophore which is produced generally by Bacillus spp. B. licheniformis K11 could produce the siderophore most highly after 96 h of incubation under nutrient broth at
with initial pH 9.0. For the production of the
, trehalose and
were the best carbon and nitrogen sources in Davis minimal medium, respectively. The
was Produced in M9 medium (pH 9.0) after 4 days at
, and purified from culture broth of B. licheniformis K11 by using Amberlite XAD-2, Sephadex LH-20 column chromatography, and reversed-phase HPLC. The
had the biocontrol activity against spore germination of P. capsici and F. oxysporum on potato dextrose agar (PDA). The results indicate that the
is an antifungal mechanism of B. licheniformis K11 against phytopathogenic fungi.
Inhibition of Browning in Yam Fresh-cut and Control of Yam-putrefactive Bacterium Using Acetic Acid or Maleic Acid.
Ryu, Hee-Young ; Kwun, In-Sook ; Park, Sang-Jo ; Lee, Bong-Ho ; Sohn, Ho-Yong ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 135~141
To increase the consumer acceptability of yam and the shelf-life of fresh-cut yam, organic acid-treated fresh-cut yam was prepared. When uncontaminated fresh-cut yam was stored at
for 14 days after treatment with 1% (v/w) organic acids, the browning and microbial putrefaction of fresh-cut yam were inhibited by treatment of acetic acid or maleic acid, whereas treatment of citric acid and ascorbic acid, commonly used browning inhibitors in food industry, did not show apparent effects on the browning and putrefaction of yam. The Inhibitory effects of acetic acid or maleic acid were superior than those of NaOCl (100 ppm), hydrogen peroxide (100 ppm) or commercially available washing solution. Also, treatments of 1% acetic acid, or 1% maleic acid Into artificially-contaminated yam
showed strong inhibition of browning and putrefaction during long term storage at
. The growth inhibition test indicated that 0.1% is enough to inhibit the growth of psychrotrophic yam-putrefactive Pseudomonas sp., and treatment of 0.1% acetic acid, or 0.1% maleic acid inhibited the browning and microbial putrefaction of fresh-cut yam. Our results suggested long-term distribution of yam or other root crops products is possible by treatment of organic acid, such as acetic acid, combined with aseptic vacuum packaging technology.
Characteristics of Citrus By-Product Ferment Using Bacillus subtilis as Starter Extracts.
Moon, Young-Gun ; Lee, Kyeong-Jun ; Heo, Moon-Soo ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 142~149
In this study, we investigated the biological activity of antioxidant and antibacterial activity of citrus by-product ferment. Hot water extracts and 80% methanol extracts from citrus byproduct of ferment were screened for antibacterial activity pathogenic bacteria by paper disc method. Among the various hot water extracts and 80% methanol extracts the Bacillus subtilis showed relatively strong antibacterial activities in the order. The reducing activity on the 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and
radical scavenging potential were sequentially screened, in search for antioxidant activities of citrus by-product ferment.
Bioremediation of Oil-Contaminated Soil Using an Oil-Degrading Rhizobacterium Rhodococcus sp.412 and Zea mays.
Hong, Sun-Hwa ; Park, Hae-Lim ; Ko, U-Ri ; Yoo, Jae-Jun ; Cho, Kyung-Suk ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 150~157
The advanced bioremediation of diesel-contaminated soil through the exploration of bacterial interaction with plants was studied. A diesel-degrading rhizobacterium, Rhodococcus sp.412, and a plant species, Zea mays, having tolerant against diesel was selected. Zea mays was seeded in uncontaminated soil or diesel-contaminated soil with or without Rhodococcus sp. 412. After cultivating for 30 days, the growth of Zea mays in the contaminated soil inoculated with Rhodococcus sp. 412 was better than that in the contaminated soil without the bacterium. The residual diesel concentrations were lowered by seeding Zea mays or inoculating Rhodococctis sp. 412. These results Indicate that the simultaneous use of Zea mays and Rhodococcus sp. 412 can give beneficial effect to the remediation of oil-contaminated soil. Bacterial community was characterized using a 16S rDNA PCR and denaturing gradient gel electrophoresis (DGGE) fingerprinting method. The similarities of DGGE fingerprints were
between the uncontaminated soil and diesel contaminated soil. The similarities of DGGE fingerprints were
between the uncontaminated soil samples, and
between the diesel-contaminated soil samples. This results indicated that the structure of bacterial community was significantly influence by diesel contamination.
Monitoring of Methicillin Resistant Staphylococcus aureus from Medical Environment in Korea.
Kwon, Young-Il ; Kim, Tae-Woon ; Kim, Hae-Yeong ; Chang, Yun-Hee ; Kwak, Hyo-Sun ; Woo, Gun-Jo ; Chung, Yun-Hee ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 158~162
Methicillin-resistant Staphylococcus aureus (MRSA) is one of a major nosocomial pathogen worldwide and the emergence of this strain has become a major clinical problem. This study was performed for 13 hospitals with more than 400 beds in the country by collecting samples including hands and nasal cavities of doctors, nurses, guardians and patients. Also, additional 320 samples of hands and nasal cavities of 160 community resident in different locations and regions were collected. In all of medical environments and community resident, 625 strains of S. aureus were detected. Among 625 strains of S. aureus, 585 strains(93.6%) showed the resistance to at least one kind of antimicrobial and 112 strains (17.9%) showed multi-drug resistance with the resistance to 4 different types of antimicrobial. Total 152 MRSA strains (24.3%) were isolated from medical environment and community resident. In nasal cavity and hand, 49 MRSA (19.4%) and 103 (27.6%) MRSA were isolated, respectively Minimum inhibitory concentration(MIC) test is used to measure for susceptibility of MRSA isolated to oxacillin. At a concentration
of oxacillin, 11 strains were inhibited. 32 strains at
, 41 strains at
, 3 strains at
, 25 stains at
and 40 strains at over
were inhibited. It was considered that medical environment showed higher than livestock and marine environments in MRSA detection rate.
Qualitative Analysis of
in Germanium-Fortified Yeast.
Kim, So-Yeun ; Kim, Myoung-Hee ; Woo, Hee-Gweon ; Kim, Bo-Hye ; Sohn, Tsang-Uk ; Jung, Jin-Wook ; Baek, Dae-Heoun ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 163~172
This study was designed to investigate that inorganic germanium
did not exist in germanium-fortified yeast or obtained to non-detectable value by current analytical methods and equipments. For this purpose, we achieved
qualitative analysis protocol which could be the scientific basis of the study. Since reddish brown precipitate was formed from the reaction of
with 1 equiv
, and dark brown precipitate was also formed from the reaction of
with 2 equiv
was qualitatively analyzed by observing these particular colored-precipitates. Because no color change was showed from the reaction between
, the color change could be caused by charge transfer transition on Ge-O and B binding properties. The reaction between
and germanium-fortified yeast did not show any color change and precipitate formation which meant no
existed in germanium-fortified yeast. The reaction between
and supernatant specimen collected from the outside of dialysis membrane (MWCO 1,200 dalton) did not show any color change and precipitate formation. Therefore, we considered that the both germaniums in and outside of the dialysis membrane were organic germaniums. Germanium-fortified yeast which was biosynthesized organic germanium can be applied not only as a new functional material for improving health, prevention and treatment of chronic degenerative diseases including cancers, and the regulation of immune system, but also as a new materials.
Characteristics and HMG-Co A Reductase Inhibitory Activity of Fermented Red Pepper Soybean Paste(Kochujang) Prepared from Red-Rice and Barley.
Hyun, Kwang-Uk ; No, Jae-Duck ; Lim, Seong-Il ; Cha, Seong-Kwan ; Choi, Sin-Yang ;
Microbiology and Biotechnology Letters, volume 35, issue 2, 2007, Pages 173~176
The purpose of this study was to improve the palatability of fermented red pepper soybean paste (Kochujang). Five individual models of Kochujang were prepared the following: control, glutinous rice containing 50% (A) and 100% (B) of barley, glutinous rice containing 1.5% (C) and 3.0% (D) of red rice. These Kochujangs were prepared and fermented for 60 days at 30 in a crock, and examined for changes in their physicochemical properties. There were no distinguishable change in their pH, acidity, formol-N content. L (lightness)-, a (redness)- and b (yellowness)-values were decreased rapidly until 20 days of fermentation. After 60 days, the values of L-, a- and b- of A, B Kochujang were increased than those of control, C, D Kochujang. Sensory profiles of color, flayer, taste and overall quality on the final products which fermented for 60 days showed that there were no differences in quality of each models. HMG-Co A reductase inhibitory activities were observed in 1.5% of red rice containing Kochujang.