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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 35, Issue 4 - Dec 2007
Volume 35, Issue 3 - Sep 2007
Volume 35, Issue 2 - Jun 2007
Volume 35, Issue 1 - Mar 2007
Selecting the target year
Effects of Plants, Rhizobacteria and Physicochemical Factors on the Phytoremediation of Contaminated Soil
Hong, Sun-Hwa ; Cho, Kyung-Suk ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 261~271
Phytoremediation is an economic and environmentally friendly technique to remediate contaminated-soil. In this study, the effects of plants, rhizobacteria and physicochemical factors on phytoremediation have been reviewed. For successful phytoremediation, the selection of plants is primarily important. To remediate soil contaminated with petroleum hydrocarbon, raygrass (Lolium multiflorum lam), white mustard, vetch (Vicia villosa), tall fescue (Festuca arundinacea), legumes, poplar, and Pine (Pinus densiflora) were mainly applied, and the removal efficiency of petroleum hydrocarbon were ranged 68 to 99%. Corn (Zea mays), raygrass (Lolium multiflorum lam), vetch (Vicia villosa), mustard, clover (Trifolium repens), and tall fescue (Festuca arundinacea) were used for the removal of polycyclic aromatic hydrocarbon, and their removal efficiencies were 50-98%. Rhizobacteria play significant roles for phytoremediation because they can directly participate in the degradation of contaminant as well as promoting plants growth. The following rhizobacteria were preferred for phytoremediation: Azospirillum lipoferum, Enterobactor cloacae, Azospirillum brasilense, Pseudomonas putida, Burkholderia xenovorans, Comamonas testosterone, Pseudomonas gladioli, Azotobacter chroococcum, Bacillus megaterium, and Bacillus subtilis. Pysicochemical factors such as pH, temperature, nutrient, electron acceptor, water content, organic content, type of contaminants are consequential limiting factors for phytoremediation.
Screening of the Genes Expressed in Pichia pastoris Grown in Phosphate-Limited Chemostat Culture
Hong, Ji-Yeon ; Ahn, Jung-Oh ; Park, Myoung-Soo ; Choi, Soon-Yong ; Choi, Eui-Sung ; Jung, Joon-Ki ; Lee, Hong-Weon ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 272~277
The physiological responses of microorganisms to specific nutrient limitation can be regulated at the transcriptional levels. In this study, in order to develop the Pichia pastoris-derived promoter inducible by nutrient-limited condition, we constructed cDNA libraries using RT-PCR of total RNA from P. pastoris in steady-states of phosphate-limited chemostat with different dilution rates. Various genes were detected from cDNA library. Among these genes, the gene encoding putative sodium/phosphate (
/Pi) symporter (NPS), high affinity transporter of phosphate, was detected. It was observed that expression of NPS increased in a manner specific to phosphate-limited condition through Northern blot. Therefore, it is thought that the promoter from NPS gene may have the potential as auto-inducible promoter by phosphate-limited culture condition without inducer.
A Novel Screening Strategy for Salt-resistant Alpha-helical Antimicrobial Peptides from a Phage Display Library
Park, Ju-Hee ; Han, Ok-Kyung ; Lee, Baek-Rak ; Kim, Jeong-Hyun ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 278~284
A novel screening strategy for salt-resistant antimicrobial peptides from a M13 peptide library was developed. Fusion of MSI-344, a magainin derivative and indolicidin to pIII coat proteins did not significantly affect viability of the recombinant phages, which indicated that the pIII could neutralize toxicity of the antimicrobial peptides and therefore it is possible to construct antimicrobial peptide library in Escherichia coli. On the basis of the conserved sequence of
-helical antimicrobial peptides, a semi-combinatorial peptide library was constructed in which the peptides were displayed by pIII. To remove hemolytic activity from the library, the phages bound to red blood cells were removed, and the subtracted phage library was screened for binding to target bacteria Pseudomonas aeruginosa and Staphylococcus aureus under high salt concentrations. The screened peptides showed relatively low antimicrobial activity against the target bacteria. However, antimicrobial activities of the screened peptides P06 and S18 were not affected by the cation concentrations of 150 mM
, 2 mM
and 2 mM
without significant hemolytic activity. This screening strategy that is based on binding capacity to target cells provides new potential to develop salt-tolerant antimicrobial peptides.
Optimization of the Reaction Conditions for (R)-Phenyl-1,2-ethanediol Preparation by Recombinant Epoxide Hydrolase from Caulobacter crescentus
Lee, Ok-Kyung ; Kim, Hee-Sook ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 285~291
Enantioconvergent hydrolysis process for the preparation of chiral diol from racemic epoxides by using the recombinant Caulobacter crescentus epoxide hydrolase (CcEH) in Escherichia coli BL21 (DE3) was optimized. For the optimization, the effects of detergent, temperature and product inhibition on the enantiopurity and the yield of diol were investigated. (R)-phenyl-1,2-ethanediol with 92% enantiomeric excess and 56% yield from 20 mM racemic styrene oxide was obtained by using the recombinant CcEH at the optimal condition of
and the addition of 2% (w/v) Tween 80. At 50 mM racemic styrene oxide was used as a substrate, (R)-phenyl-1,2-ethanediol was obtained with 87% enantiomeric excess and 77% yield. Racemic phenyl-1,2-ethanediol, (R)-phenyl-1,2-ethanediol and (S)-phenyl-1,2-ethanediol dramatically inhibited the hydrolytic activity of the recombinant CcEH. These results suggested that another EH with the regioselectivity on
-position of (R)-enantiomer and without feedback inhibition by products would be needed as the partner EH of C. crescentus EH.
Influence of High Light and Nitrate Deprivation on the Carotenoid Biosynthesis in Haematococcus pluvialis
Yun, Ji-Hyun ; Kwak, In-Kyu ; Jin, Eon-Seon ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 292~297
The unicellular green alga, Haematococcus pluvialis used as a biological production system for astaxanthin. It accumulates large amounts of the red ketocarotenoid astaxanthin when exposed to various environmental stress such as active oxygen species and high light intensities. To induce astaxanthin biosynthesis of H. pluvialis, cells were incubated in either nitrate free at
under continuous high light intensity (
) for 2 days or high light stress only. Expressions of astaxanthin biosynthetic genes such as carotenoid hydroxylase, IPP isomerase and
-carotene ketolase were monitored under different culture conditions by using real time RT-PCR. All the subjected genes increased their expression under highlight and N-deprivation condition where a large amount of astaxanthin was accumulated.
Effect of NaCl on Halomonas subglaciescola DH-1 Incapable of Growing at Non-Salinity
Na, Byung-Kwan ; Yoo, Young-Sun ; Park, Doo-Hyun ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 298~303
A halophilic bacterium, H. subglaciescola DH-1, grew at 2.0 M salinity, but did not grow at 0.8 M salinity when cultivated at higher temperature (
) than optimum (
). When the cell extract of strain DH-1 was heated at
for 60 min in the absence of NaCl, isocitrate dehydrogenase and malate dehydrogenase lost their activities, but when it was heated in the presence of 2.0 M NaCl, the activity was maintained. Meanwhile, the cell extract of E. coli did not catalyze the reduction of
to NADH coupled with the oxidation of isocitrate and malate at higher salinities than 1.0 M. The pH range for DH-1 was 7 to 10, and that for E. coli was 5 to 9. DH-1 was not grown in conditions with sodium salts other than NaCl.
Optimization of Culture Conditions of Bacillus pumilus JB-1 for Chungkook-jang Fermentation in Soybean Boiling-Waste Liquor Medium
Kwon, Ha-Young ; Ryn, Hee-Young ; Kwon, Chong-Suk ; Lee, Sang-Han ; Sohn, Ho-Yong ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 304~309
Soybean is useful source of protein, especially in Asia. But soybean needs heat inactivation or fermentation process before consumption, since it contains the toxic lectin and various protease inhibitors. Therefore, production of soybean boiling-waste liquor (SBWL) as a byproduct is inevitable. In this study, the chemical composition of SBWL and the optimization of culture conditions for Bacillus pumilus JB-1, a selected strain for functional chungkuk-jang fermentation, using SBWL were investigated. The SBWL contains 88% water, 9.5% free sugar, 1.6% crude protein, 0.3% crude fat, 0.1% crude fiber and 2.1% ash, respectively. The contents of total polyphenol, total flavonoids and free amino acid in SBWL were 55%, 76%, and 30% of those of raw soybean, respectively. Culture conditions for B. pumilus JB-1 in SBWL were optimized. The 1/10-diluted, 0.1 % of
added SBWL without pH adjustment and carbon source addition was cultured at
for 48 h with agitation (120 rpm). The 0.5% of inoculation was enough. The large scale fermentation in 5-L jar fermentor showed that the SBWL is a good resource for production of chungkuk-jang starter and functional ingredients.
Identification and Fermentation Characteristics of Lactic Acid Bacteria Isolated from the Fermentation Broth of Korean Traditional Liquor, Andong-Soju
Bae, Kyung-Hwa ; Shin, Kee-Sun ; Ryu, Hee-Young ; Kwon, Chong-Suk ; Sohn, Ho-Yong ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 310~315
To investigate the effect of lactic acid bacteria in Andong-Soju fermentation and traditional nuruk maturation, several lactic acid bacteria were isolated from the Andong-Soju fermentation broth and traditionally matured nuruks using Lactobacilli MRS agar containing bromocresol purple. Among the isolated bacteria, ADS-L1 showed the highest lactic acid production and was dominant species in fermentation broth. Based on physiological characteristics and 16S rDNA sequencing results, the ADS-L1 was identified as Pediococcus acidilactici. The ADS-L1 grew well at
, and under the acidic conditions at pH 4, whereas the ADS-L1 failed to grew by treatments of 12% (w/v) ethanol or 0.01N HCl. Considering the high temperature of nuruk above
during nuruk maturation and the high ethanol concentration of broth above 12% at the end-stage of Andong-Soju fermentation, these results suggested that the ADS-L1 is popular in matured nuruks and plays role in the early-stage of fermentation. Analysis of pH, brix, reducing sugar content, lactic acid production, and cell growth during the cultivation of ADS-L1 further suggested that the ADS-L1 may contribute the prevention of contamination by rapid and steady acidification of broth, and do not cause problems by rapid death at the end-stage of fermentation.
The Effects of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts
Yang, Eun-Soon ; Hong, Ran-Hi ; Kang, Sang-Mo ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 316~324
We studied the effects of genistein obtained from glycolysis of genistin, a kind of phytoestrogen present in soybeans, on cell proliferation and type I pN collagen synthesis in normal human dermal fibroblasts(NHDF). Cell proliferation was increased significantly with genistein treatment at 54-year aged NHDF. Genistein increased cell proliferation more strongly in cells form old doner than young doner. The senescence-associated
-galatosidase activity was decreased in NHDF from 77-year old doner with genistein treatment. Type I pN collagen synthesis was increased with genistein treatement in UVA treated and non-treated NHDF. The increasement of collagen synthesis was more effective in aged cells than young cells. Type I pN collagen synthesis was also increased with genistein treatment in collagen matrix culture with NHDF from sun-exposed and non-exposed skin from 54-year old doner. Genistein treatment inhibited MMP-1 synthesis in old NHDF but not in young NHDF. In conclusion, genistein may be a useful agent for preventing intrinsic aging as well as photoaging.
Characteristics of Bacterial-Koji and Doenjang(soybean paste) Made by using Bacillus subtilis DJI
Chang, Mi ; Chang, Rae-Choon ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 325~333
One bacterium with high proteinase production and spore-forming ability was isolated from korean traditional soybean paste(doenjang). The isolated strain was identified as Bacillus subtilis, based on gram-staining, biochemical properties and l6S rRNA gene sequencing, and designated as B. subtilis DJI. Its growth rate was very fast, and it reached its stationary phase within 9 h, and then started to form spores. Bacterial-koji and doenjang were prepared using B. subtilis DJI. Chemical components of the doenjang were determined after 2 months of aging period: amino nitrogen 507 mg%, crude protein 14.3%, crude fat 4.8% and water 54.9%. The composition of total and free amino acids and their ratios of doenjang were changed during the aging period. Among total amino acids in DJI doenjang, glutamic acid, aspartic acid, leucine and arginine were the major amino acids. The fibrinolytic activities of DJI doenjang and traditional doenjangs were 909.7 units/ml and
, respectively. Flavor compounds of DJI doenjang and traditional doenjang were extracted by SDE(simultaneous steam distillation and extraction), and analyzed by GC/MS; DJI doenjang possessed the typically favorable flavor compounds in traditional korean doenjang, with reduced off-flavor compounds.
Comparative Characterization on Bacterial Disinfection Using Trichloroisocyanuric Acid, Bromochlorodimethylhydantoin, and Calcium Hypochlorite
Oh, Se-Hyun ; Yoo, Hyun-Duk ; Lee, Han-Na ; Kim, Eung-Soo ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 334~338
The disinfection effects of trichloroisocyanuric acid (TICA), calcium hypochlorite (CH), and Bromochlorodimethylhydantoin (BCDMH) on various bacteria in aqueous suspension were comparatively characterized at various concentrations and exposure times of each disinfectant. When various bacteria cells (
) including Escherichia coli, Pseudomonas aeroginosa, Enterococcus faecalis, Bacillus cereus, Legionella pneumophila, and Staphylococcus aureus were exposed with a solution containing 8 ppm each of TICA, a 99% of the initial cells were killed in 60 sec, 368 sec, 372 sec, 506 sec, 812 sec, and 909 sec, respectively. In addition, the minimum exposure time required to kill 99% of E. coli (
) with 8 ppm of each TICA, BCDMH and CH was measured at 60 sec, 114 sec, and 7,100 sec, respectively. These comparative studies demonstrate that disinfection efficacy is highly variable depending on microbial species as well as disinfectant type, concentration, and exposure time.
Characterization of Bacteriocin-Like Substances Produced by Bacillus subtilis MJP1
Yang, Eun-Ju ; Chang, Hae-Choon ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 339~346
The MJP1 bacterial strain, which possesses antifungal activity, was isolated from meju and identified as Bacillus subtilis based on its morphological and biochemical properties, as well as its 16S rRNA sequence. Antimicrobial activity was found against various species of Gram-positive bacteria, yeasts, and molds, including food-spoilage microorganisms. The antifungal activity was found to be stable after heat and proteolytic enzyme treatment, and in the pH range of
. The antibacterial activity was stable in the pH range of
, but about 50% of the activity was lost after 24 hr at
. The antibacterial compound was also inactivated by proteolytic enzyme treatment, indicating its proteinaceous nature. The apparent molecular masses of the partially purified antifungal and antibacterial compounds, as indicated by using the direct detection method in Tricine-SDS-PAGE, were approximately 2.4 kDa and 4.5 kDa, respectively. These studies suggest that B. subtilis MJP1 produces two bacteriocin-like substances with antifungal and antibacterial activities.
Phylogenetic Differentiation of Streptomyces spp. Isolated from Potato Scab Lesions in Jeju Island of Korea on the Basis of 16S rRNA Gene Sequences
Lee, Soo-Hyun ; Ko, Young-Hwan ; Kim, Chang-Jin ; Kim, Bum-Joon ; Lee, Keun-Hwa ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 347~351
Potato scab is prevalent in all potato-growing areas of Jeju Island and causes economically significant losses. Streptomyces species are known as pathogens of potato scab. In this study, we analyzed the 16S rRNA sequences of Streptomyces spp, which are isolated from potato scab lesions in Jeju Island, and constructed 16S rRNA phylogenetic tree. All isolates were clearly differentiated into the genus Streptomyces, and the tree also showed that new scab-causing Streptomyces spp or not yet named species of Streptomyces are existed in Jeju Island, Korea.
Characterization of a Lactobacillus acidophilus Strain Isolated from Korean Infant Feces and Cloning of Surface Layer Protein Gene slp and Its Expression in Escherichia coli
Park, Myeong-Soo ; Ji, Geun-Eog ; You, Kwan-Hee ; Lee, Si-Kyung ; Jeong, Won-Seok ; Kim, Jin-Hyung ; Jo, Myoung-Hwan ; Kim, Soo-Young ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 352~356
A Lactobacillus sp. has been isolated from infant feces and characterized according to its physiological properties and identified as Lactobacillus acidophilus KLA1012. A gene coding surface layer protein (SLP) has been cloned and the sequence has been determined. The nucleotide sequence of slpA was 1,338 bp in size and was identical to that of L. acidophilus ATCC 4356 (100%). Amino acid sequence of SLP-A was deduced from the nucleotide sequence and it had signal sequence at N-terminal, consisting of positively charged amino acid mainly lysine. slpA was cloned and heterologously expressed in E. coli M15 and the 45.2 kDa surface-layer protein band was examined by SDS-PAGE and confirmed by Western blotting using polyclonal antibody against L. acidophilus KLA 1012 SLP-A protein.
Isolation and Identification of Fungal Strains Producing Gibberellins from the Root of plants
Rim, Soon-Ok ; Lee, Jin-Hyung ; Khan, Sumera Afzal ; Lee, In-Jung ; Rhee, In-Koo ; Lee, Kyung-Soo ; Kim, Jong-Guk ;
Microbiology and Biotechnology Letters, volume 35, issue 4, 2007, Pages 357~363
249 fungal strains were isolated from the roots of 26 plants, and the production of GAs was spectrophotometric ally examined. As a result 76 fungal strains were shown to produce GAs. Bioassay of culture broth from seventy six fungal strains producing GAs was carried out with waito-c rice, that is dwarf rice. The seventy six fungi with GAs-producing activity were incubated for seven days in 40 mL of Czapek's liquid medium at
and 180 rpm, and the culture broth of fungi were treated on the 2-leaf rice sprout. Fifteen of these showed plant growth promoting activity and the amount of each GAs in the medium was measured by Gas chromatographymass spectrometer (GC-MS). Nine of these fungi were also identified by genetic analysis of the nucleotide sequences in the internal transcribed spacer region of the ribosomal DNA.