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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Microbiology and Biotechnology Letters
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Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 36, Issue 4 - Dec 2008
Volume 36, Issue 3 - Sep 2008
Volume 36, Issue 2 - Jun 2008
Volume 36, Issue 1 - Mar 2008
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Evaluation of Lactic Acid Bacterial Community in Kimchi Using Terminal-Restriction Fragment Length Polymorphism Analysis
Shim, Sang-Min ; Lee, Jong-Hoon ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 247~259
Terminal-restriction fragment length polymorphism (T-RFLP) analysis, one of rapid culture-independent microbial community analysis methods, was used to determine the lactic acid bacterial complexity and dynamics during kimchi fermentation at
. At both temperatures, the common presence of Leuconostoc mesenteroides, Lc. inhae, Lc. kimchi, Weissella koreensis, W. cibaria, Lactobacillus sakei, Lb. curvatus, Lb. plantarum, Lb. paraplantarum, Lb. pentosus, and Lb. brevis was predicted. Lc. citreum and Enterococcus faecalis were detected at
, respectively. W. koreensis predominated during the mid stage of kimchi fermentation whereas lactobacilli were dominants during later stage. Lb. sakei and Lb. curvatus became dominants regardless of fermentation temperature but the growth of Lb. plantarum, Lb. paraplantarum, Lb. pentosus, and Lb. brevis was restricted at psychrophilic temperature. Some species of leuconostocs were maintained until the later stage of kimchi fermentation.
Evaluation of Novel Constitutive Expression Vectors Equipped with Mined Promoters from Metagenome
Han, Sang-Soo ; Kim, Geun-Joong ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 260~267
The choice of expression vector is very important for industrial production of proteins. Therefore, the systematic mining of promoters over a wider range of genetic resource and/or host is required. We previously reported a novel bidirectional reporting system (pBGR) for the isolation of promoters from metagenome and screened useful promoters that functioned constitutively in E. coli under general culture conditions. Among them, three promoter sequences including each upstream region were amplified by PCR and used to construct new expression vectors. To facilitate subcloning, a multi-cloning site was incorporated into the downstream region of the revere primer sequence. At these sites, GFP, esterase and
-glucosidase were subcloned and analyzed the constitutive expression ability of new promoter in terms of protein solubility and expression level. As a result, these vectors expressed the proteins constitutively to a level of
of the total cell protein in soluble fraction (>80 %). This study suggested that excavation of metagenomic promoters for construction of expression vector in a certain strain could provide a way for the development of the expression systems.
Selection of the Auxin and ACC Deaminase Producing Plant Growth Promoting Rhizobacteria from the Coastal Sand Dune Plants
Lim, Jong-Hui ; Kim, Jong-Guk ; Kim, Sang-Dal ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 268~275
In order to develop the multi-functional rhizobacteria that can exert positive effect on the growth of plants growing in the coastal sand dune located along East Coast of Korea, rhizospheral bacteria of 11 different plants from this area were isolated 1,330 rhizobacteria. Among these, 23 strains were able to produce auxin and had spectrum of antagonism toward various phytopathogenic microbes. To know the mechanism of this antifungal activity, these 23 strains were subjected to further analyses; 19 strains of these produced siderophore as determined by color reaction on CAS-blue plate, 4 strains produced antifungal cellulase as judged by color change on CMC-Congo red plate, 17 strains were able to utilized insoluble phosphate salts, also determined by clear zone formation on PVK medium. Identification of the strain was assigned to all 23 strains by l6s rDNA sequence analysed, and all were identified to be in the genus of Bacillus and Pseudomonas. One strain of these, denoted Pseudomonas fluorescens IB4-14, showed ACC deaminase activity which is known to be involved in the resistance of environmental stress such as salt and drought. Also, P. fluorescens IB4-l4 showed the germination stimulation and roots growth promoting activity on the in vivo assay of Lysimachia mauritiana Lam. (spoonleaf yellow loosestrife).
Antifungal Activity of Lactobacillus plantarum Isolated from Kimchi
Yang, Eun-Ju ; Chang, Hae-Choon ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 276~284
A lactic acid bacterium having antifungal activity was isolated from kimchi. It was identified as Lactobacillus plantarum based on its morphological and biochemical properties, and 16S rRNA sequence, and designated as Lb. plantarum AF1. This isolate inhibited the growth of Aspergillus flavus ATCC 22546, A. fumigatus ATCC 96918, A. petrakii PF-1, A. ochraceus PF-2, A. nidulans PF-3, Epicoccum nigrum KF-1, and Cladosporium gossypiicola KF-2 under a dual culture overlay assay. Also, the antimicrobial activity was found to be active against various species of Gram-positive and Gram-negative bacteria. The antifungal activity was found to be stable after heat (
, 15 min) and proteolytic enzyme treatment, but it was unstable over pH 5.0. The antifungal compound(s) was estimated to have a low molecular mass (below 3,000 Da).
Physicochemical Properties of an Anti-Yeast Substance Produced by Rahnella aquatilis Strain AY2000
Kang, Min-Jung ; Lee, Bok-Kyu ; Lee, Eun-Woo ; Kim, Kwang-Hyeon ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 285~290
Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS has a declining tendency during storage. To investigate what has been decreased activity of the AYS, the AYS was treated with various physicochemical agents in this paper. The activity of AYS was decreased by heat treatment. Thiol reagent such as
-mercaptoethanol or dithiothreitol was also another factor decreasing the activity of AYS. However, pH, EDTA, and NaCl were not factors decreasing the activity of AYS. Use of methanol to precipitate the AYS was also decreased the activity of AYS. The activity of AYS was not lost after Sepharose S-400 gel filtration. However, the AYS activity was completely lost, when a polysaccharide and a unknown substance (230 nm absorption) among components of the AYS was separated by DEAE-cellulose chromatography. MIC of the AYS against S. cerevisiae was usually determined at
Changes of Angiotensin I-Converting Enzyme Inhibitory Activity, Fibrinolytic Activity and
-Secretase Inhibitory Activity of Red Wines During Fermentation and Post-Fermentation
No, Jae-Duck ; Lee, Eun-Na ; Seo, Dong-Soo ; Chun, Jong-Pil ; Choi, Shin-Yang ; Lee, Jong-Soo ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 291~298
The cardiovascular angiotensin I-converting enzyme inhibitory activity, fibrinolytic activity and bbb-secretase inhibitory activity of four kinds of red wine were investigated during fermentation and post-fermentation. After 10 days of fermentation, the antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activities of all the red wines ranged from 38.6% to 58.8%. However, the ACE inhibitory activities increased with the prolongation of the post-fermentation period; moreover, in the Vitis hybrid red wine, the ACE inhibitory activity reached its highest value, 76.9%, after 120 days of post-fermentation. During the fermentation and post-fermentation of all the red wines, fibrinolytic activity was weak or not detected. After 10 days of fermentation, Vitis labrusca B red wine exhibited the greatest antidementia
-secretase inhibitory activity of 54.8%, though
-secretase inhibitory activity decreased significantly to less than 10% during 120 days of post-fermentation. In conclusion, we obtained a highly valuable Vitis hybrid red wine that was fermented for 10 days at
with Vitis hybrid and S. cerevisiae K-7 and then post-fermentation for 120 days at
Development of Egg Yolk Antibody Specific to the Pancreatic Lipase Domain for Anti-Obesity
Woo, Seung-Eun ; Kwon, Jin-Hyuk ; Yang, Si-Yong ; Park, Hyun-Ju ; Kim, Hyung-Kwoun ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 299~306
Human pancreatic lipase is a digestive enzyme which is synthesized in pancreas, secreted into small intestine, and there hydrolyze the fat in food. Pancreatic lipase protein composes of catalytic domain and colipase-binding domain. In this research, the gene segments corresponding to total protein, catalytic domain, and co lipase-binding domain were cloned by PCR method, inserted into an expression vector, and then used to transform Escherichia coli BL21 (DE3). The recombinant proteins produced were purified and injected intramuscularly three times into laying hens. The egg yolk antibodies (IgY) were obtained from the egg yolks and tested for their antibody titer. Among three IgY, the IgY against colipase-binding domain showed the highest antibody titer. All three IgY had inhibitory effects on the porcine pancreatic lipase. Among them, the IgY against colipase-binding domain showed the highest inhibition effects. The fat diet with corn oil and IgY was administrated to the experimental rats and their blood compositions were examined with time course. The triglyceride concentration of treated rats was decrease meaningfully when compared with those of control rats. This suggested that the IgY against colipase-binding domain antigen inhibited pancreatic lipase in vivo.
Production of Xylooligosaccharides by Yeast Cell Surface-Displayed Endoxylanase
Kim, Hyun-Jin ; Lee, Jae-Hyung ; Kim, Yeon-Hee ; Nam, Soo-Wan ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 307~313
The yeast surface expression system, pCTXYN (6.8 kb), of Bacillus endoxylanase gene (xynB, 642 bp) was constructed and introduced into Saccharomyces cerevisiae EBY100 cell. The transformed yeast cell showing the highest endoxylanase activity was selected through the active staining of colonies grown on YPDG medium containing xylan. With the yeast transformant, EBY100/pCTXYN, grown on galactose containing medium, it was found that the endoxylanase was successfully displayed on the yeast cell surface and the xylooligosaccharides were efficiently produced from xylan. The most of endoxylanase activity was detected in the cell fraction and reached about 1.9 unit/mL after 48 h cultivation. The optimized conditions for xylooligosaccharides production from xylan were determined as follows: substrate and its concentration, oat spelt xylan 6%; concentration of yeast whole-cell, 5 unit/mL; temperature,
, and reaction time
. When the oat spelts xylan and corncob xylan were hydrolyzed by treatment with cell surface-displayed endoxylanase, xylotriose was formed as a main product.
Fed-batch Culture of Recombinant E.coli for the Production of Penicillin G Amidase
Lee, Sang-Mahn ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 314~319
Penicillin G amidase (PGA, benzylpenicillinaminohydrolase, EC 220.127.116.11) is industrially important enzyme which converts penicillin G to 6-aminopenicillanic acid (6-APA) and phenylacetic acid (PAA). The PGA in E. coli ATCC 11105 is secreted into the periplasm after removing signal sequences and becomes heterodimer which composed of two subunits, small subunit (24 kDa) and large subunit (65 kDa). In this study, the PGA gene was obtained from E. coli ATCC 11105 using PCR (polymerase chain reaction) technique. The active PGA was successfully secreated into periplasm in E. coli BL2 1(DE3) harboring pET-pga plasmid. The optimized fed-batch fermentation, consisting of a three-step shift of culture temperature from
, gave a productivity of 19.6 U/mL with a cell growth of 62 O.D. at 600 nm.
Characterization of Anti-dementia, Cadiovascular and Antioxidant Functionalities in Korean Traditional Alcoholic Beverages
Seo, Dong-Soo ; Kim, Jae-Ho ; Ahn, Byung-Hak ; Lee, Jong-Soo ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 320~325
Some anti-dementia, cardiovascular and antioxidant functionalities of Korean traditional alcoholic beverages were characterized. Anti-dementia acetylcholinesterase inhibitory activities were generally not detected or low except BHS-ju (19.5%) and buthyrylcholinesterase inhibitory activities were also show below 1.0%. However,
-Secretase inhibitory activities were high in PMR-ju (42.5%), WJY-ju (41.6%) and SSJ-ju (42.9%). Antihypertensive angiotensin I-converting enzyme inhibitory activities was the highest in YON-ju (85.6%), however fibrinolytic activities were not detected in all traditional alcohol beverages. Furthermore, antioxidant activities were very high in SBB-ju (72.2%) and GMB-ju (67.9%), however SOD like activities generally were show below 20%.
Structural Identification of
from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper
Woo, Sang-Min ; Kim, Sang-Dal ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 326~335
The siderophore (
) of Bacillus subtilis AR18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LR-20 chromatography, and reversed-phase RPLC. The
was identified bacillibactin with its structure by GC-MS,
(bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of
showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.
Effect of Methyl tert-Butyl Ether and Its Metabolites on Microbial Activity and Diversity in Tidal Mud Flat
Cho, Won-Sil ; Cho, Kyung-Suk ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 336~342
The effect of methyl tert-butyl ether (MTBE) and its metabolites like tert-butyl alcohol (TBA), and formaldehyde (FA) on microbial activity and diversity in tidal mud flat was studied. MTBE, TBA, and FA with different concentrations were added into microcosms containing tidal mud samples, and placed at room temperature for 30 days. Then the physico-chemical properties such as pH, moisture contents and organic matter contents in the microcosms were measured. In addition, the total viable cell number and dehydrogenase activity were measured. Bacterial communities in the microcosms were monitored using a 16S rRNA-PCR-DGGE (Denaturing gradient gel electrophoresis) fingerprinting method. As a result, the exposure concentrations of MTBE and its metabolites showed no correlation with the physico-chemical factors (P>0.05). Dehydrogenase activity and total viable cell number were decreased with increasing MTBE, TBA and FA concentrations (P<0.05). The toxic effect was higher the following order: FA > MTBE > TBA. Dominant species in the microcosms contaminated with MTBE and its metabolites were Sphingobacteria, Flavobacteria, delta-proteobacteria, gamma-proteobacteria. The diversity of bacterial community was not significantly influenced by MTBE and its metabolites.
Anti-inflammatory Effect of Boswellia sacra (Franckincense) Essential Oil in a Mouse Model of Allergic Asthma
Lee, Hye-Youn ; Yun, Mi-Young ; Kang, Sang-Mo ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 343~352
Frankincense, the gum resin derived from Boswellia species, is complex mixtures composed of about
highly aromatic essential oil,
alcohol-soluble resins, and the remaining water-soluble gums. The anti-inflammatory properties of frankincense, alcohole-soluble resins, are well-recognized, but the question of whether aromatic essential oil also plays a role in the allergic asthma remains unanswered. This study was performed to evaluate anti-inflammatory effects of Boswellia sacra essential oil (BSEO) on ovalbumin (OVA)-induced asthma mouse model. BALB/c mice after intraperitoneal OVA sensitization were challenged with intratracheal OVA. One experimental group was inhaled with 0.3% BSEO for the later 8 weeks. BALB/c mice were sensitized and challenged with OVA and developed airway eosinophilia, mucus hypersecretion, and airway hyperresponsiveness. In contrast, the BSEO treated mice had reduced a number of eosinophils among BALF cells, goblet cell hyperplasia, and airway hyperresponsiveness. Cytokine analysis of BALF revealed that BSEO caused an increase in Th1 cytokine (interferon-
)) and a decrease in Th2 cytokines (interleukin-4 (IL-4), IL-5 and IL-13) levels. In addition, the OVA-specific serum IgE and eotaxin levels were also reduced. In mice inhaled BSEO,
mediastinal lymph nodes cells were also decreased. These results suggest that inhaled BSEO as a immunomodulator in Th1/Th2 mediated asthma may have therapeutic potential for the treatment in allergic airway inflammation by a simple, cost-effective way.
An Interferon Resistance Induced by the Interaction between HCV NS5B and Host p48
Park, So-Yeon ; Lee, Jong-Ho ; Myung, Hee-Joon ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 353~359
Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.
Evaluation and Improvement of Bioassay for Residual Antibiotics in Foods
Park, Min-Hee ; Kim, Tae-Woon ; Jo, Nam-Uk ; Jeong, Ji-Yoon ; Lee, Soon-Ho ; Lee, Jong-Ok ; Kim, Hae-Yeong ;
Microbiology and Biotechnology Letters, volume 36, issue 4, 2008, Pages 360~365
For the screening of residual antibiotics in foods, bioassays and microbiological inhibitor tests are commonly applied. These methods are tested by the various susceptibility of bacteria against different kinds of antibiotics. However, the sensitivity of bioassay is generally insufficient to detect some residual antibiotics at level of interest. This study was performed to investigate the detection limit of variable antibiotics of the bioassay and to improve the sensitivity to some antibiotics. The sensitivity of bioassay using Bacillus megaterium ATCC 9885, B. subtilis ATCC 6633, B. cereus ATCC 11778 and Geobacillus stearothermophilus ATCC 10149 was low in the detection of macrolides, quinolones, chloramphenicol, and monensin. On the contrary, Micrococcus luteus ATCC 9341 showed high sensitivity to macrolides and Escherichia coli ATCC 11303 was highly sensitive to quinolones and aminoglycosides. Consequently, both strains would be useful to improve sensitivity of bioassay with a wide detection range.