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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 39, Issue 4 - Dec 2011
Volume 39, Issue 3 - Sep 2011
Volume 39, Issue 2 - Jun 2011
Volume 39, Issue 1 - Mar 2011
Selecting the target year
Isolation and Characterization of Starch-hydrolyzing Pseudoalteromonas sp. A-3 from the Coastal Sea Water of Daecheon, Republic of Korea
Chi, Won-Jae ; Park, Da-Yeon ; Jeong, Sung-Cheol ; Chang, Yong-Keun ; Hong, Soon-Kwang ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 317~323
Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between
. However, it grew more slowly at the temperatures of
. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-
, Pseudoalteromonas luteoviolacea
, Pseudoalteromonas rubra
, and Pseudoalteromonas byunsanensis
, with 98.30%, 97.86%, 97.78%, and 97.25% similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-
belong to a clade. However, strain A-3 differed from P. phenolica O-
in relation to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-
and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces
-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and
, respectively. Pseudoalteromonas sp. A-3's high, stable production of
-amylase in addition to its biochemical features, such as alkalitolerance, suggest that it is a good candidate for industrial applications.
Plant Growth Promotion Activity of Endophytic Fungi Isolated from the Roots of Calystegia soldanella
You, Young-Hyun ; Yoon, Hyeok-Jun ; Woo, Ju-Ri ; Seo, Yeong-Gyo ; Shin, Jae-Ho ; Choo, Yeon-Sik ; Lee, In-Jung ; Kim, Jong-Guk ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 324~329
Eight endophytic fungal strains were isolated from the roots of Calystegia soldanella from the western coast of South Korea. The culture filtrate of the eight endophytic fungi were applied to waito-c rice seedlings in order to verify potential plant growth promotion activities. The results of bioassay indicated that the Cs-9-7 fungal strain possessed the highest plant growth promotion activity. Fungal culture filtrates were analyzed to verify secondary metabolites using gas chromatography and mass spectroscopy with selected ion monitoring (GC/MS-SIM). The culture filtrate of the Cs-9-7 fungal strain was confirmed to contain gibberellins GA3 (1.229 ng/mL), GA4 (3.535 ng/mL), GA7 (1.408 ng/mL) and GA12 (0.378 ng/mL). Polymerase chain reactions (PCR) were performed so as to determine the internal transcribed spacer (ITS) regions for the identification of isolated strains with universal primers ITS-1 and ITS-4. The Cs-9-7 fungal strain, isolated from the root of C. soldanella, has been named Aspergillus tubingensis Cs-9-7.
Isolation and Optimized Culture Conditions of Fibrinolytic Enzyme Producing Strain Isolated from Korean Traditional Soybean Sauce
Baek, Seong-Yeol ; Yun, Hye-Ju ; Park, Heui-Dong ; Yeo, Soo-Hwan ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 330~336
Bacterial strains exhibiting fibrinolytic activity were screened from traditional Korean soybean sauce. The Fibrinolytic activities of the various isolated microorganism were further examined and the superior strain YJ11-21 was selected for further analyses. Gene sequence analysis of 16S rDNA of the YJ11-21 strain revealed Bacillus licheniformis. Optimal culture conditions were investigated in order to maximize the production of the fibrinolytic enzyme by YJ11-21. Amongst the carbon sources tested, glucose was the most effective for enzyme production and amongst the nitrogen sources tested, yeast extract was seen to be the most effective. A one percent addition of NaCl to the medium resulted in the highest fibrinolytic activity. Interestingly, a 10% addition of NaCl resulted in a high activity together with a high cell growth rate. Therefore, YJ11-21 is speculated of being a halotolerant. The optimum pH and temperature for enzyme production were a pH of 9.0 and
Site-Specific Recombination by the Integrase MJ1 on Mammalian Cell
Kim, Hye-Young ; Yoon, Bo-Hyun ; Chang, Hyo-Ihl ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 337~344
Integrase MJ1 from the bacteriophage
carries out recombination between two DNA sequences (the phage attachment site, attP and the bacterial attachment site, attB) in NIH3T3 mouse cells. In this study, the integration vector containing attP, attB and the integrase gene MJ, was constructed. The integration mediated by integrase MJ1 in Escherichia coli led to excision of LacZ. Therefore, the frequency of integration was measured by the counting of the white colony, which is detectable on X-Gal plates. The extrachromosomal integration in NIH3T3 mouse cells was monitored by the expression of the green fluorescent protein (GFP) as a reporter. To demonstrate integration mediated integrase MJ1 in NIH3T3 cells, vectors containing attP and attB were co-transfected into NIH3T3 cells. The integration was confirmed by fluorescent microscopy. The expression of GFP was induced in NIH3T3 cells expressing MJ1 without accessory factors. By contrast, the excision mediated by the MJ1 between attR and attL had no effect on the expression of GFP. These results suggest that integrase MJ1 may enable a variety of genomic modifications for research and therapeutic purposes in higher living cells.
The Promoting Effect of Pleuropterus cilinervis Extracts Fermented with Lactobacillus rhamnosus on Hair Growth
Park, Jang-Soon ; Lee, Jae-Sug ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 345~349
The bacterial growth and pH of Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus during the fermentation process, were evaluated. The results indicated that the bacterial count after fermentation always remained higher than 6 log CFU/mL, with a constant pH of approximately 4. In order to evaluate the effects of Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus, on hair growth promotion, C57BL/6 mice were chosen as experimental subjects. Six week old males with similar body weights were divided into four groups; a normal group (saline), a negative control group (essence base), a positive control group and an experimental group (Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus, mixed with negative control). The substances and test materials were applied topically on the back skin of the mice for 8 days. Other external conditions and variables such as food intake were kept at the same as the four groups. At the end of the experiment it was noted that hair re-growth in the experimental group, using gross and histological examinations, was higher than that in the positive control group. This study therefore provides an empirical evidence that Pleuropterus cilinervis extracts and animal milk fermented with Lactobacillus rhamnosus promotes hair growth, and suggests that applications could be found in hair loss treatments.
Analysis of Constituents in Sipjundaebo-tangs Fermented by Lactic Acid Bacteria
Yang, Min-Cheol ; Jeong, Sang-Won ; Ma, Jin-Yeul ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 350~356
The purpose of this study was to investigate changes in the contents of constituents in Sipjundaebo-tang (SJ) and its fermentations (FSJ) with 8 species of lactic acid bacteria. Eight strains of lactic acid bacteria, Lactobacillus casei KFRI129, L. plantarum KFRI 144, L. curvatus KFRI 166, L. hilgardii KFRI 229, L. delbruekil subsp. lactis KFRI 442, L. casei KFRI 692, L. gasseri KCTC 3163, and Bifidobacterium breve KFRI 744, were utilized for the fermentation of Sipjundaebo-tang. As a result we identified three constituents which increased and two constituents which decreased. The increased constituents were isolated by chromatographic techniques and then their structure elucidated using NMR and MS. The decreased constituents were confirmed by comparing standard compounds. These compounds were quantitatively analyzed using the HPLC/DAD system. The increased constituents were identified to be cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5), while the decreased constituents were liquiritin (2) and nodakenin (4). Generally, liquiritin (2) and nodakenin (4) were noted as having decreased in fermented Sipjundaebo-tangs (FSJs), while cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5) were seen to have increased. Sipjundaebo-tang fermented by L. plantarum KFRI144 exhibited the most remarkable changes in all of fermentations. The eight lactic acid bacteria all demonstrated differing decomposable rates on the five maker compounds in fermented Sipjundaebo-tang (FSJ).
Quality Characteristics of wheat Nuruk and Optimum Condition of Liquid Starters for Aspergillus sp.
Choi, Jeong-Sil ; Jung, Seok-Tae ; Kim, Joo-Yeon ; Choi, Ji-Ho ; Choi, Han-Seok ; Yeo, Soo-Hwan ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 357~363
This study focus was primarily the development of liquid starters for Aspergillus oryzae and Aspergillus niger prepared with wheat bran as a low cost culture medium. For the preparation of the liquid media wheat bran was added at rates of 0, 5, 10, 15 and 20% and the Aspergillus sp. strains were then inoculated to these prepared broths. The results indicated that the more that wheat bran was contained in the medium, the more mycelia was produced for A. oryzae and A. niger. The highest enzyme activities were obtained with a 10~15% adding rate of wheat bran for both strains. Changes in the enzyme activities of the liquid starters during various incubation times (0, 24, 48, 72 and 96 hrs), indicated that the highest enzyme activities were seen between 48 and 72 hrs of culture. In addition, a comparative study was carried out on the production of enzymes using wheat as a substrate in nuruk, with liquid starter made from fermented agents according to the same concentrations used with the wheat bran. The pH, acidity, amino acidity, reducing sugar content and enzyme activity (
-amylase, glucoamylase, acidic protease) of wheat nuruk made with liquid starter were compared with those of wheat nuruk made with solid starter. The results suggest that the liquid starter is superior in both cases.
Establishment of Functional Cells for Vascular Defect Disease from Human Embryonic Stem Cell via Region Sorting Depending on Cell Volume
Lee, Ji-Hye ; Kim, Ju-Mi ; Chung, Hyung-Min ; Chae, Jung-Il ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 364~373
Human embryonic stem cells have been highlighted as a valuable cellular source in the regenerative medicine field, due to their pluripotency. However, there is the challenge of the establishment of specific functional cell type forms of undifferentiated human embryonic stem cells (hESC). To establish and purify functional cell types from hESCs, we differentiated undifferentiated hESCs into vascular lineage cells and sorted the specific cell population from the whole cell population, depending on their cell volume, and compared them with the non-sorted cell population. We observed that about 10% of the PECAM positive population existed in the VEGF induced differentiating human embryoid body (hEB), and differentiated hEBs were made into single cells for cell transplantation. After making single cells, we performed cell sorting using a fluorescence-activated cell sorter (FACs), according to their cell volume on the basis of FSC region gating, and compared their therapeutic capacity with the non-sorted cell population through cell transplantation into hindlimb ischemic disease model mice. 4 Weeks after cell transplantation, the recovery rate of blood perfusion reached 54% and 17% in the FSC regions of sorted cells- and non-sorted cells, respectively. This result suggests that derivation of a functional cell population from hESCs can be performed through cell sorting on the basis of cell volume after preliminary differentiation induction. This approach may then greatly contribute to overcoming the limitations of marker sorting.
Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm (Eisenia fetida) using DGGE and Pyrosequencing
Kim, Eun-Sung ; Hong, Sung-Wook ; Chung, Kun-Sub ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 374~381
The beneficial effects of Eisenia fetida on soil properties have been attributed to their interaction with soil microorganisms. The bacterial diversity of the intestinal tract of E. fetida was investigated by culture-dependent and culture-independent methods including denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analyses. In a pure culture, Lysinibacillus fusiformis (51%), Bacillus cereus (30%), Enterobacter aerogenes (21%), and L. sphaericus (15%) were identified as the dominant microorganisms. In the DGGE analyses, B. cereus (15.1%), Enterobacter sp. (13.6%), an uncultured bacterium (13.1%), and B. stearothermophilus (7.8%) were identified as the dominant microorganisms. In the pyrosequencing analyses, Microbacterium soli (26%), B. cereus (10%), M. esteraromaticum (6%), and Frigoribacterium sp. (6%) were identified as the dominant microorganisms. The other strains identified were Aeromonas sp., Pseudomonas sp., Borrelia sp., Cellulosimicrobium sp., Klebsiella sp., and Leifsonia sp. The results illustrate that culture independent methods are better able to detect unculturable microorganisms and a wider range of species, as opposed to isolation by culture dependent methods.
Electricity Production Performance of Single- and Dual-cathode Microbial Fuel Cells Coupled to Carbon Source and Nitrate
Jang, Jae-Kyung ; Lee, Eun-Young ; Ryou, Young-Sun ; Lee, Sung-Hyoun ; Hwang, Ji-Hwan ; Lee, Hyung-Mo ; Kim, Jong-Goo ; Kang, Youn-Koo ; Kim, Young-Hwa ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 382~386
Microbial fuel cells (MFC), devices that use bacteria as a catalyst to generate electricity, can utilize a variety of organic wastes as electron donors. The current generated may differ depending on the organic matter concentrations used, when other conditions, such as oxidant supply, proton transfer, internal resistance and so on, are not limiting factors. In these studies, a single-cathode type MFC (SCMFC) and dual-cathode type MFC (DCMFC) were used to ascertain the current's improvement through an increase in the contact area between the anode and the cathode compartments, because the cathode reaction is one of the most serious limiting factors in an MFC. Also an MFC was conducted to explore whether an improvement in electricity generation resulted from oxidizing the carbon sources and nitrates. About 250 mg
sodium acetate was fed to an anode compartment with a flow rate of 0.326 mL
by continuous mode. The current generated from the DCMFC was higher than the value produced from MFC with a single cathode. COD removal of dual-cathode MFC was also higher than that of single-cathode MFC. The nitrate didn't affect current generation at 2 mM, but when 4 and 8 mM nitrate was supplied, the current in the single-cathode and dual-cathode MFC was decreased by 98% from
mA, respectively. These results demonstrate that increasing of contact area of the anode and cathode can raise current generation by an improvement in the cathode reaction.
Cytyotoxicity and Anti-Malassezia Activity of Limonene
Lee, Jeong-Hyun ; Lee, Jae-Sug ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 387~389
A previous study of ours indicated that Citrus auranifoli oil possesses antifungal activity against Malassezia furfur and Malassezia pachydermatis. In this study, we evaluated the anti-M. furfur and M. pachydermatis activities of limonene, which is a major component of C. aurantifolia oil, using the disk diffusion method. We also examined cytotoxicity against human normal epithelial (Beas-2B) cells using the cytopathic effect reduction (CPE) method. The results revealed that the minimum fungicidal concentration (MFC) value of limonene is lower than the value for itraconazole. The MFC value of limonene was seen to be 7.81
/mL against M. furfur and 3.90
/mL against M. pachydermatis. MFC values of itraconazole against M. furfur and M. pachydermatis were 62.50
/mL and 31.25
/mL, respectively. In addition, it was noted that limonene was not toxic to Beas-2B cells with normal morphology at a concentration of 100
/mL. However, itraconazole exhibited weak toxicity at the same concentration. Therefore, our results indicate that limonene could potentially be effective at controlling M. furfur and M. pachydermatis infections with no cytotoxicity.
Characterization of the Bacteriocin Produced by a Leuconostoc mesenteroides Strain Inhibiting the Growth of Lactobacillus sakei
Lee, Kwang-Hee ; Lee, Jong-Hoon ;
Microbiology and Biotechnology Letters, volume 39, issue 4, 2011, Pages 390~396
Lactobacillus sakei is known to be the most populous lactic acid bacteria in over-ripened kimchi. Twenty three strains of Leuconostoc species inhibiting the growth of Lb. sakei were isolated from kimchi and amongst these the Leuconostoc mesenteroides strain CK0122 exhibited the highest antagonistic activity against Lb. sakei. The culture supernatant of the strain CK0122 was fractionated by a molecular weight cutter and lyophilized. The fraction with a molecular weight of less than 3,000 Da showed antagonistic activity against Lb. sakei. The antibacterial activity of the active fraction was sensitive to proteinase K treatment, confirming its proteinaceous nature (bacteriocin). The crude bacteriocin was active in the pH range of 4 to 7 and extremely stable after 15 min of heat treatment at
. The crude bacteriocin inhibited the growth of Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Alcaligenes xylosoxydans, Flavobacterium sp., and Salmonella typhimurium.