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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 4, Issue 4 - Dec 1976
Volume 4, Issue 3 - Sep 1976
Volume 4, Issue 2 - 00 1976
Volume 4, Issue 1 - 00 1976
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Studies on the Hesperidinase of Aspergillus niger S-1
Microbiology and Biotechnology Letters, volume 4, issue 4, 1976, Pages 131~137
Aspergillus niger S-1 was proved to be a good hesperidinase producer which have been selected for naringinase utilization. Enzyme of this strain had good characteristics and purified relative high degree with good recovery by ammonium sulfate or aceton treatment. Results obtained were summarized as follows (1) The enzyme was most active at 60
, when the reaction was performed in the pH 4.0 for 30min. Optimum pH for enzyme activity was 5.0 and activity was retained 78％ at pH value 3.5. (2) Hesperidinase activity retained 95％ of its full activity after treatment at 60
for 30min at pH value 4.0., 70％ at 70
and 65％ at 80
. Most stable pH of this enzyme was showed 5.0 after treatment for 24hr at 4
(3) Only Magnesium ion activated enzyme reaction, while other metallic ions, Cu
＋＋/ inhibited. (4) Eleven fold purification with 35％ recovery was obtained in the case of 60％ aceton treatment and 10-fold purification with 5.6％ recovery was showed with 40％ aceton comparing to the crude extract Enzyme. (5) Crude enzyme precipitated with 0.4-0.6 saturated ammonium sulfate contained 13f6 of the original enzyme activity with 48-fold increase in specific activity and enzyme has been purified 25 fold with a yield 19％ by 0.6-5.8 saturation. (6) Hesperidinase formation was noticeably increased by addition of small amount of orange-peel extraction on the wheat bran medium.
Studies on the Microbial Glucose Isomerase Part 1. Isolation and Characterization of Streptomyces species Producing Glucose Isomerase
Chung, Tai-Wha ; Kim, Hyun U. ; Moon H. Han ;
Microbiology and Biotechnology Letters, volume 4, issue 4, 1976, Pages 138~144
five strains of Streptomyces spp. with high Productivity of glucose isomerase (15-30 units/ml) were obtained among 280 microbial strains isolated from 150 soil samples. These strains produced glucose isomerase with xylose as an inducer. These 5 strains were also identified to be different strains of Streptomyces spp.：streptomyces sp. K-14, K-53, K-71, K-77 and K-733. It was found that Streptomyces sp. K-14 produced the highest enzyme activity. The spore chains of these strains were rectiflexible and spore surface was smooth except Steptomyces sp. K-77 and K-733, with spiny surface.
Studies on the Microbial Glucose Isomerase Part 2. Culture Conditions of Streptomytes sp. K-14 in Producing Glucose Isomerase
Tai Wha Chung ; Moon H. Han ;
Microbiology and Biotechnology Letters, volume 4, issue 4, 1976, Pages 145~151
Cultural characteristics of a strain of Streptomyces sp. K-14 (KFCC 35051) producing glucose isomerase were demonstrated. The glucose isomerase was produced when the strain was grown in the medium containing pure xylan or xylan.containing materials such as wheat bran or com cob. The optimum condition was attained in a culture medium composed of 3 % wheat bran or com cob, 2 % com steep liquor, 0.1%
and 0.012 %
for the production of the glucose isomera,e. The production of the enzyme reached to a maximum level when the strain was cultured for 40 hrs
and pH 7.0.
Studies on the Fermentative Utilization of Cellulosic Wastes (part III) Production of Yeast from the Hydrolyzate of Rice straw, Rice hull and Corn Starch Pulp.
Microbiology and Biotechnology Letters, volume 4, issue 4, 1976, Pages 152~158
Cultivation condition of yeast on the utilization of fermentable substrate from the cellulosic wastes such as rice hull, rice straw and corn starch cake was investigated. The results obtained were summarized as follows；1. Corn starch cake was respectively added to rice hull and rice straw in order to increase sugar concentration in the hydrolyzate, and then hydrolyzed. As the result, concentration of sugar in hydrolyzed solution of rice hull was 9.12％, in that of rice straw was 7.98％. 2. It was found that calcium carbonate as a neutralizer was the most effective to prepare the culture broth of yeast. 3. An optimal growth of Hansenula subpelliculosa GFY-2 was observed in the medium prepared by adding 0.3％ of ammonium sulfate, 0.4％ of potassium phosphate dibasic, 0.02％ of magnesium sulfate, sodium chloride and calcium chloride to hydrolyaed sugar solution, respectively. 4. Hansenula subpellicuiosa GFY-2 cultured in the substrate solution which of rice hull and rice straw added to corn starch cake was assimilated more than 90％ of sugar in the hydrolyzate within 48 hours. The yeast cells yielded in rice hull was 46.5％, and that of rice straw 45.4％ to utilized sugars.
Enzymatic Chillproofing and Beer Foam Stability Part III. Effects of Papain Concentration and Storage Condition
Pack, M.Y. ;
Microbiology and Biotechnology Letters, volume 4, issue 4, 1976, Pages 159~165
Freshly fermented green beer samples were chillproofed with reduced amounts of papain in order to avoid the deterioration of foam quality. Without being pasteurized, the bottled beer samples were stored at different temperatures for various day and then their foam stabilities were determined. A significant improvement in foam stability was observed in the beer chillproofed with 15 ppm of papain for 22 days at
and not pasteurized. The findings may be applied in the finishing processes for draft beer where pasteurization is not needed.
Taxonomic Study of Bacillus coagulans by Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization Technique
Chung, Chi-Kwan ;
Microbiology and Biotechnology Letters, volume 4, issue 4, 1976, Pages 166~178
Taxonomic study of 11 strains of Bacillus coagulans and 14 strains of 13 spccies of Bacillus by deoxyribonucleic acid (DNA)-DNA hybridization were conducted. Among the 11 strains of B. coagulans, 6 were isolated from soil and the rest were the authentic strains obtained from American Type culture collection (ATCC) or the Institute for Fermentation, Osaka (IFO). All strains were examined to confirm as they are expected species of B. coagulans by the methods of Cordon et al. according to Bergey's Manual (8th ed.). The intraspecific DNA homology indexes among the 11 strains of B. coagulans using strain ATCC 7050 as the standard (
H labeled input DNA) showed 76％ or, more, respectively. These findings accorded well with the results of the conventional taxonomic study according to the Bergey's Manual. The interspecific DNA homology indexes between B. coagulant strain ATCC 7050 and the type cultures of B. subtilis (168), B. licheniformis (IFO 12107), B. pumilus (IFO 12110), B. firmus (ATCC 14575), B. lentus (ATCC 10840), B. circulans (ATCC 4513), B. macelans (ATCC 8244), B. polymyxa (ATCC 842), B. sphaericus (ATCC 14577), B. brevis (ATCC 8246, IFO 12334), B. laterosporus (ATCC 64), and B. pantothenticus (ATCC 14576) respectively, showed 2 to 4％, while that of between B. coagulans ATCC 7050 and Escherichia coli K-12 was less than 1 ％.