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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Microbiology and Biotechnology Letters
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Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 41, Issue 4 - Dec 2013
Volume 41, Issue 3 - Sep 2013
Volume 41, Issue 2 - Jun 2013
Volume 41, Issue 1 - Mar 2013
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Isolation of Yeasts from Wild Flowers in Gyonggi-do Province and Jeju Island in Korea and the Production of Anti-gout Xanthine Oxidase Inhibitor
Hyun, Se Hee ; Mun, Hye Yeon ; Lee, Hyang Burm ; Kim, Ha-Kun ; Lee, Jong-Soo ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 383~390
DOI : 10.4014/kjmb.1309.09002
Several yeasts were isolated from flowers found in Gyonggi-do Province and Jeju island in Korea. They were then identified by a comparison of their PCR-amplified D1/D2 regions of 26S rDNA, internal transcribed spacer 1 and 2 inclusive of 5.8S rDNA, using the BLAST database. A total of fifty four yeast strains were isolated from wild flowers in Gyonggi-do and the genus Pseudozyma was noted as being dominant. A total of thirty two strains were isolated from Songaksan and Seongsan-ilchulbong in Jeju island and Sporobolomyces ruberrimus was seen to be dominant. The anti-gout xanthine oxidase inhibitory activities of the culture broths and cell-free extracts from eighty six yeast strains were then determined. The cell-free extracts of Pseudozyma hubeiensis 228-S-1 exhibited the highest xanthine oxidase inhibitory activity of 19.6%. The XOD inhibitor was also maximally produced when Pseudozyma hubeiensis 228-S-1 was cultured at
for 36h in YEPD medium.
Enzymatic Characterization of Lactococcus lactis subsp. lactis Cyclomaltodextrinase Expressed in E. coli
Jang, Myoung-Uoon ; Kang, Hye-Jeong ; Jeong, Chang-Ku ; Park, Jung-Mi ; Yi, Ah-Rum ; Kang, Jung-Hyun ; Lee, So-Won ; Kim, Tae-Jip ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 391~397
DOI : 10.4014/kjmb.1312.12001
A putative cyclomaltodextrinase (LLCD) gene was cloned from the genome of Lactococcus lactis subsp. lactis KCTC 3769 (ATCC 19435), which encodes 584 amino acids with the predicted molecular mass of 68.7 kDa. KCTC 3769 shares approximately 40% of amino acid sequence identity with the CDase-family of enzymes. The dimeric enzyme with C-terminal six-histidines was heterologously expressed and purified from recombinant E. coli. LLCD showed the highest activity against
-cyclodextrin (CD) at pH 7.0 and
. In particular, LLCD exhibited extremely low activity against starch and pullulan, while its CD-hydrolyzing activity was about 80 times higher than starch. Due to its much higher activity on CD over starch, LLCD has been identified as a member of CDases. However, LLCD can be distinguished from the other common CDases on the basis of its extremely low hydrolyzing activity against starch, pullulan, and acarbose.
Statistical Optimization of Culture Conditions for the Production of Aphicidal Metabolites of Beauveria bassiana Bb08
Go, Eunsu ; Lim, Younghoon ; Jeong, Hyeongchul ; Choi, Jaepil ; Park, Inseo ; Kim, Jeong Jun ; Lee, Dong-Jin ; Kim, Keun ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 398~406
DOI : 10.4014/kjmb.1309.09006
For the maximal production of aphicidal metabolites produced by the Beauveria bassiana Bb08, statistical methods such as the Box-Behnken experimental design and response surface methodology were used. The fungal culture filtrate was sprayed towards 3-star aphids and the mortality was examined. After the statistical analysis of the aphid mortality, the optimal culture conditions were found to be a culture temperature of
, medium pH 5.9, flask shaking speed of 209.0 rpm, and culture time of 5.9 days. The expected mortality on days 4, 5, and 6 after spraying the filtrate on to the aphids were 76.8%, 84.9%, and 89.4%, respectively. All 4 factors of the culture conditions significantly affected the production of the aphicidal metabolites, and the order of significance was temperature, pH, culture time and shaking speed.
Cellular Antioxidant Activity and Whitening Effects of Dendropanax morbifera Leaf Extracts
Park, Su Ah ; Park, Jun ; Park, Chan Il ; Jie, Young Jong ; Hwang, Yun Chan ; Kim, Yong Hyun ; Jeon, So Ha ; Lee, Hye Mi ; Ha, Ji Hoon ; Kim, Kyeong Jin ; Park, Soo Nam ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 407~415
DOI : 10.4014/kjmb.1311.11001
In this study, we investigated the antioxidant activities on HaCaT and the whitening effects on B16F1 melanoma cells of Dendropanax morbifera leaf extract. In an antioxidative activity assay using HaCaT cells, the ethyl acetate (
) and aglycone fractions (
) of the D. morbifera leaf extract didn't exhibit any characteristics of cytotoxicity. When HaCaT cells were exposed to a single large dose (
) of UVB, the extracts protected the cells against UVB radiation. When HaCaT cells were treated with 10 mM
rose bengal, the ethyl acetate (
) and aglycone (
) fractions protected the cells against oxidative damage in a concentration dependent manner. When the whitening effects of D. morbifera leaf extract were tested in melanoma B16/F1 cells treated with the a-melanocyte stimulating hormone (
-MSH), the extracts inhibited
-MSH-stimulated intra/extracellular melanogenesis in a concentration dependent manner. The inhibitory effects of the ethyl acetate and aglycone fractions of D. morbifera leaf extract were 21% and 44% at
, respectively. Both are more effective than arbutin (15% at
) which is known as a whitening agent. These results indicate that fractions of the D. morbifera leaf can function as cell protectants and natural antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging
and other ROS, and protecting cells against ROS. In addition, fractions of the D. morbifera leaf can be applied to new whitening cosmetics because of their inhibitory effects on
-MSH stimulated melanogenesis in B16F1 melanoma cells.
A Comparison of the Components and Biological Activities of the Ethanol Extracts of Achyranthes japonica Nakai and Achyranthes bidentata Blume
Lee, Ye-Seol ; Kim, Mi-Sun ; Kim, Duck-Jin ; Sohn, Ho-Yong ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 416~424
DOI : 10.4014/kjmb.1307.07001
In the course of a study for the development of functional foods utilizing Woosul (Achyranthis radix), the components and various biological activities of Korean Woosul (AJN: Achyranthes japonica Nakai) and Chinese Woosul (ABB: Achyranthes bidentata Blume) were compared. Woosul in Korea, including AJN and ABB, are regulated and part of the Korean Pharmacopoeia. From AJN and ABB, ethanol extracts and their subsequent organic solvent fractions were prepared and their in-vitro antimicrobial, antioxidant and anti-diabetes activities were evaluated. Although AJN and ABB have no clear distinction in terms of usage in Korea, our results suggest that AJN has higher quantities of lipid-soluble components and lower amounts of water-soluble sugars than does ABB. ABB also appears to possess greater amounts of flavonoid and polyphenol substances than AJN. Analyses of biological activities showed that the fractions of AJN were more active as antibacterial agents, and possessed more pronounced
-glucosidase inhibitory activities than those of the ABB fractions. However, the antioxidant activities of the ABB fractions, as determined by DPPH anion-, ABTS cation-, nitrite-scavenging activity and reducing power, were higher than those of the AJN fractions. Our results suggest that the components and bioactivity of the extracts and subsequent fractions of AJN and ABB are different. Therefore, usage of either AJN or ABB should be carefully considered, as regards their individual properties, when the active fractions of Woosul are employed in the development of functional foods or novel plant-derived medicines.
Induction of G2/M Arrest and Apoptosis by the Methanol Extract of Typha orientalis in Human Colon Adenocarcinoma HT29 Cells
Jin, Soojung ; Yun, Seung-Geun ; Oh, You Na ; Lee, Ji-Young ; Park, Hyun-Jin ; Jin, Kyong-Suk ; Kwon, Hyun Ju ; Kim, Byung Woo ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 425~432
DOI : 10.4014/kjmb.1308.08002
Typha orientalis, also known as bulrush or cattail, is a perennial herbaceous plant found in freshwater wetlands and has been widely used in constructed wetlands for wastewater treatment. Recent data has revealed that SH21B, a mixture composed of seven herbs including T. orientalis, exhibited an anti-adipogenic activity by the inhibition of the expression of adipogenic regulators. However, the anti-cancer effect of T. orientalis and its molecular mechanisms remain unclear. In this study, we evaluated the anti-cancer effect and its mechanism in the methanol extract of T. orientalis (METO) on human colon carcinoma HT29 cells. It was found that METO treatment showed cytotoxic activity in a dose-dependent manner, and induced G2/M cell cycle arrest and apoptosis in HT29 cells. The induction of G2/M arrest by METO was associated with the up-regulation of phospho-Cdc2 (Tyr15), an inactive form of Cdc2 and the down-regulation of Cdc25c phosphatase. METO also induced tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) expression. In addition, METO-induced apoptosis was characterized by the proteolytic activation of caspase-3, degradation of poly ADP ribose polymerase (PARP), and up-regulation of death receptor FAS and pro-apoptotic Bax expression. Collectively, these results indicate that the cell cycle inhibition and apoptosis induction of METO in HT29 cells allows for the possibility of its use in anti-cancer therapies.
Anti-Oxidative, Anti-Inflammatory, and Anti-Melanogenic Activities of Endlicheria Anomala Extract
Jin, Kyong-Suk ; Lee, Ji Young ; Kwon, Hyun Ju ; Kim, Byung Woo ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 433~441
DOI : 10.4014/kjmb.1309.09005
In this study, the anti-oxidative, anti-inflammatory, anti-melanogenic activities of Endlicheria anomala (Nees) Mez methanol extract (EAME) were evaluated by use of in vitro assays and cell culture model systems. The results revealed that EAME scavenges various radicals such as 1,1-diphenyl-2-picryl hydrazyl hydrogen peroxide induced reactive oxygen species, and lipopolysaccharide induced nitric oxide. Furthermore, EAME induced the expression of anti-oxidative enzymes such as heme oxygenase 1, thioredoxin reductase 1, NAD(P)H dehydrogenase 1, and their upstream transcription factor, nuclear factor-E2-related factor 2. Moreover, EAME inhibited in vitro DOPA oxidation and 3-isobutyl-1-methylxanthine induced melanogenesis in B16F10 cells. Its anti-melanogenic activity will have originated from the inhibition of tyrosinase enzyme activity and melanogenesis related protein expression. Taken together, these results provide the important new insight that E. anomala possesses various biological activities such as anti-oxidative, anti-inflammatory, and anti-melanogenic. Therefore, it might be utilized as a promising material in the fields of nutraceuticals and cosmetics.
Isolation of the Alcohol-Tolerant Lactic Acid Bacteria Pediococcus acidilactici K3 and S1 and their Physiological Characterization
Jang, Danbie ; Park, Seulki ; Lee, Hyunjoo ; Pyo, Sangeun ; Lee, Han-Seung ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 442~448
DOI : 10.4014/kjmb.1308.08001
Lactic acid bacteria (LAB) are a representative group of probiotics and used in many fermented foods and beverages. Several recent studies have shown that LAB are present in makgeolli which is a traditional Korean alcoholic beverage. However, most LAB are intolerant of more than 6% (v/v) alcohol concentrations. For this reason, alcohol-tolerant LAB are isolated from kimchi, makgeolli and nuruk using alcohol containing selective media. After being cultured in MRS broth containing 13% (v/v) alcohol, the two strains which showed the highest increasing O.D values, were finally selected. As results of 16S rRNA gene sequencing and biochemical characterization using an API kit, the two species were identified as Pediococcus acidilactici K3 and S1. In addition, the identified two strains produced bacteriocins against Staphylococcus aureus. When compared with the P. acidilactici type strain, the two selected strains possessed two to three time higher growth on 12-13% (v/v) alcohol containing MRS broth. The viability of P. acidilactici K3 and S1 when inoculated in makgeolli and stored at
did not decrease through a period of one month indicating that the selected strains can be used for LAB containing makgeolli.
Anti-wrinkling Effects of Juniperus rigida Sied
Jun, Hye-Ji ; Lee, Soo-Yeon ; Kim, Jeung-Hoan ; An, Bong-Jeun ; Lee, Jin-Young ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 449~455
DOI : 10.4014/kjmb.1307.07004
Human skin is constantly exposed to environmental conditions such as UV rays, polluted air, and chemical products. UV rays, in particular, affect skin in many ways causing wrinkles, fine wrinkles, rough skin, and xeroderma through a skin aging process. The purpose of this study was to investigate the anti-wrinkling effect of Juniperus rigida Sieb., derived from a common cedar tree found the world over. Measuring the elastase to investigate wrinkling efficacy, it was shown that at a concentration level of
of the two extracts, the water extract exhibited a lower than 10% inhibition activity, while the ethanol extract exhibited a 68.5% inhibition activity. Collagenase inhibition activity in the water extract and ethanol extract were 44.9% in the former and 97.2% in the latter extract, which in the case of the ethanol extract, is similar to ascorbic acid (99.6%). Moreover, measuring the biosynthesis of collagen by fibroblast, a concentration level of
of ethanol extract produced 151.52% of biosynthetic promotion, proving that the ethanol extract acts as a superb anti-wrinkling agent. The result of an investigation conducted on the influence of the ethanol extract on MMP-1 caused by UVA showed that at a concentration level of
of the ethanol extract of J. rigida Sieb a 67.1% inhibition activity was noted. At a concentration level of
of the ethanol extract of J. rigida Sieb a 35% and 39% inhibition ratio to MMP-1 protein and mRNA were observed respectively, thereby restraining the appearance of the collagen breakdown enzyme MMP-1 and wrinkle creation by skin photo-aging.
Real-time Fluorescence Assay of DNA Polymerase Using a Graphene Oxide Platform
Gang, Jongback ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 456~461
DOI : 10.4014/kjmb.1310.10003
Using the different adsorption properties of ssDNA and dsDNA to GO, this study used a real time and efficient fluorescence assay to detect the enzymatic activity of the Klenow fragment with the adsorbed DNA to GO. Results showed that adsorption of fluorescein-tagged ssDNA to GO resulted in fluorescence quenching and DNA was released from GO by adding complementary DNA. In addition, fluorescence restoration was increased through a polymerization reaction by the Klenow fragment in the presence of a fluorescein-attached template, GO, and primer. Gel electrophoresis was conducted to confirm the hybridization and DNA polymerization reactions on GO.
Analysis of the Bacterial Community in Ojingeo-jeotgal and Selection of Bacillus Species Inhibiting the Growth of Food Pathogens
Kim, Hye-Rim ; Han, Seulhwa ; Lee, Bitnara ; Jeong, Do-Won ; Lee, Jong-Hoon ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 462~468
DOI : 10.4014/kjmb.1307.07005
Jeotgal is a generic term given to the high-salt-fermented seafood of Korea. This study aimed at developing an overview of the bacterial community present in Ojingeo-jeotgal, a highly consumed type of jeotgal, which is made with squid. Bacteria were isolated and purified from two samples on six different kinds of media and identified by 16S rRNA gene sequence analysis. Among the 121 total isolates, the most dominant genus was Bacillus, followed by coagulase-negative staphylococci (CNS) and lactic acid bacteria (LAB). CNS were detected in both samples, but LAB were observed in only a single sample. Six strains of Bacillus species inhibiting the growth of food pathogens, Staphylococcus aureus and Vibrio parahaemolyticus, were selected from the 121 isolates. These were found to inhibit the growth of both pathogens in addition to displaying proteolytic activities on media containing 6% NaCl and 2% skim milk.
Antibacterial Activity Evaluation of Radioisotope Lu-177 with a Modified Tube on Plate Core
Joh, Eun-Ha ;
Microbiology and Biotechnology Letters, volume 41, issue 4, 2013, Pages 469~471
DOI : 10.4014/kjmb.1311.11006
In this study, we measured the anti-bacterial activity of radioisotope Lu-177 using a new laboratory instrument. The disk method used for the measurement of existing anti-bacterial antibiotics is drug diffusion into the medium. To measure the antimicrobial activity of a radioisotope, a new type of laboratory instrument is necessary to prevent the drug from spreading and the present invention was thus tested. In the medium, a space isolated separately for radioisotope injection was used to prevent the radioisotope from spreading and radioisotopes are actually injected by this system. We found that the antibacterial activity increased according to the radiation dose increases. It is expected that, through the present study, measuring the antibacterial activity of the other radioisotopes easily in the laboratory will be possible.