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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
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Volume & Issues
Volume 5, Issue 4 - Dec 1977
Volume 5, Issue 3 - Sep 1977
Volume 5, Issue 1 - Mar 1977
Volume 5, Issue 2 - 00 1977
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Studies on Microbial Utilization of Agricultural Wastes (Part 7) Scale up Production of Cellulosic Single Cell Protein
Kho, Yug-Hee ; Lee, Key-Joon ; Bae, Moo ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 47~47
For the production of cellulosic single cell protien from agricultural wastes by Cellulomonas flavigena KIST 321, batch cultivation in 10l ferementor were carried out to establis', the optimal culture conditions based on the previous report. The results obtained were summarized as follows. 1. A considerable ammount of organic acids were produced in the culture broth during the batch cultivation with rice straw as substrate. The yield of biomass and digestibility of the substrate remarkable decreased at low than pH 6.0 of the culture medium. 2. The cell mass productivity could be determined indirectly by alkali consumption during the batch cultivatin by maeans of a good parallelity between alkali consumption and cell mass production.3. The optimum pH and temperature of the cultivation was observed at 7.4 and
. 4. The optimal aeration was between 0.4~0.6 vvm on the agitation of 200 rpm.
Studies on Microbial Glucose-6-phosphate Dehydrogenase(I) Isolation and Charcterization of a Strain of Leuconostoc mesenteroides Producing Glucose-6-phosphate Dehydroenase
Lee, Kyung-Eun ; Chung, Tai-Wha ; Mheen, Tae-Ick ; Han, Moon-Hi ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 53~53
Leuconostoc mesenteroides No. 20-2-10 isolated from Kimchi demonstrated to be a good strain for the production of glucose-6-phosphate dehydrogenase, an intracellular enzyme (average 1u/ml broth). It was demenstrated that the maximum enzyme activity was obtained in the AC medium containing 1.2% sucrose as a carbon source and 1.0% yeast extract and 1.0% tryptone as nitrogen sources. The optimum temperature and pH for the culture of the organism were
and 7.0, respectively. The maximum growth of the cell and the productivity of the enzyme was attained within 10 hours at the condition described above.
Biological Active Substance Produced by a Strain of Streptomyces sp.
Song, Bang-Ho ; Seo, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 61~61
An Actinomycetes isolated from soil, which produced a piscicidal substance against various kinds of fresh water fishes, identified with Streptomyces umbrosus. The taxonomic relationships on the physiological properties and morphological characteristics of the genera in the Streptomyceteceae; aerial mass color, melanoid pigment production, reverse side pigment, presence of soluble pigment, spore chain morphology, structure of spore surface and utilization of carbon sources, are presented.
Proteinase Production by Nylon Oligomers utilizting Bacteria, Achromobacter guttatus
Rhee, In-Koo ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 67~67
Extracellular proteinase was produced by Achromobacter guttatus, which was isolated from soil as a strain decomposing nylon oligomers The culture conditions and proteinase production were studied. The maximum productin of proteinase was obtained in a medium containing 0.5% nylon oligomers, 0.3% peptone, and 0.1% yeast extract with salt mixture for 24hr. Enzyem production was significantly stimulated by the addition of yeast extract. The repression of enzyme production was observed by the additon of glucose to the media.
Studies on the Glucose Isomerizing Enzyme (Part 5) Conditions for the Production of Glucose Isomerzing Enzyme of Streptomyces sp. K-17
Rhee, In-Koo ; Seo, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 73~73
A source of xylose was required for the improved profunction of glucose isomerizing enzyme of Streptomyces sp. K-17. In order to supply indirectly expensive xylose as an inducer in the cheaper form of xylan, a source of xylose from natural products such as wheat bran and barley bran was investigated for enzyme production. The eznyme productivity was increased in the containing 0.5% wheat bran and 0.5% barley bran. The bacteria was able to utilize (
to the medium. Effect of organic nitrogen sources such as yeast extract, peptone and corn steep liquor on the enzyme productin was observed. Only corn steep liquor among them stimulated the enzyme production.
Studies on the Production of Yeast Part III. Enhancement of Ribonucleic Acid Content in Yeasts
Park, Kun-Young ; Lee, Jong-Chi ; Cho, Hong-Yeon ; Yang, Han-Chul ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 79~79
During the course of investigation on the productin of RNA, it has bee found that the RNA content was in wide distribution in various kinds of yeasts, and Cryptococcus laurentii had especially high RNA content and high dry cell weight. Time courses of the RNA synthesis and dry cell weitht of Cryptococcus laurentii were examined, and the most desirable results were obtained at 8 hours of cultivation on a rotary shaker at
under the pH 5.6. Under the above condition, the RNA content and the yield of dry cells were examined changing the medium composition in various ways. Glucose was observed favorable as a carbon source, and yeast extract and meat extract as a nitrogen source. The optimum C/N ratio of the medium was 8:1. The optimum concentrations of
, NaCl and
were 0.1%, 0.4% and 0.1%, respectively. Glutamic acid mother liquor, Zn ion or A1 ion was added to the medium. Addition of A1 ion by 50 ppm showed relatively favorable effects as compared with that of the other materials in increasing the RNA content. Comparing with the basal medium, the RNA content on dry basis was increased from 18% to 23% in the final medium, whereas the yield of dry cells maintained a high amount at the level of 26 to 28 mg per ml of culture broth.
Antibiotics and Antimetabolite
Park, Boo-Kil ;
Microbiology and Biotechnology Letters, volume 5, issue 2, 1977, Pages 89~89