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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 5, Issue 4 - Dec 1977
Volume 5, Issue 3 - Sep 1977
Volume 5, Issue 1 - Mar 1977
Volume 5, Issue 2 - 00 1977
Selecting the target year
Mutagenicity of Pesticides in the Salmonella System
Microbiology and Biotechnology Letters, volume 5, issue 4, 1977, Pages 159~165
20 pesticides induding 11 insecticides, 5 herbicides and 4 fungicides have been tested for mutagenic activity in the Salmonella microsome system. It was found that Captan showed strong mutagenic activity directly in TA 1535, TA 100 and TA 98 strains, indicating that it induces both base substitution and frame shift mutation. With microsomal activation system, mutagenicity of Captan was slightly decreased. Micut, Dimethoate and Triforine revealed slight mutagenicity in TA100 without microsmal enzyme activtion.
Studies on Microbial Utilization of Agricultural Wastes (Part IX) Analsis of Cellulosic Single Cell Protein and Their Amino Acid Patterns
Microbiology and Biotechnology Letters, volume 5, issue 4, 1977, Pages 167~169
Cellulosic single cell proteins produced from rice straw by three strains of cellulolytic bacteria were analysed for their composition and for their amino acid patterns. It was showed that they contains 52.2∼70.3％ of crude Protein, 11.6∼13.9％ of crude fat and 11.5∼18.1％ of ash. The sulphur containing amino acids and threonine were analysed to be the limiting amino acid in the cellulosic SCP. The protein scores were calculated as 80.0 for Cellulomonas flavigena KIST 321, 78.00 for Cellulomonas fimi and 61.8 for Cellulomonas aurogena KIST 11. The nutritional value of the cellulosic SCP is discussed from the results.
Studies on the Isolation and Identification of Lactic Acid Bacteria and its Utilization for Pharmaceutical Preparation
Microbiology and Biotechnology Letters, volume 5, issue 4, 1977, Pages 171~175
A microbial strain capable of producing lactic acid was isolated and identified as Streptococcus faecium. During the incubation of the isolated bacterium in a synthetic medium (Petterson broth), the optimal temperature was 36 to 39
and the cell concentration at stationary growth phase was 2.1
8/ viable cells/ml. When it was dried in vacuum and diluted with avicel, the Viability of Streptococcus faecium in physiological saline solution was decreased to 80％ after incubation for 48 hr at 37
, whereas the viability was above 90％ after incubation for 1 hr at 40
and the viability in M buffer solution (pH 4.5∼9.0) at 37
was above 95％. From these data it was concluded that the isolated microbe must be adoptible for pharmaceutical preparation such as solid dosage form.
Purification and Properties of Arylsulfatase of Serratia marcescens
Yim, Moo-Hyun ;
Microbiology and Biotechnology Letters, volume 5, issue 4, 1977, Pages 177~184
Arylsulfatase catalyzes the release of SO
4//sup2/- from sulfate esters of simple phenols. Arylsolfatase occurs widely in animal tissues and in microorganisms including soil bacteria. Its widespread distribution suggests that it has a rather fundamental function and environmental meaning. It has been shown previously that arylsulfatase of Klebsiella was purified and characterized. A condition of arylsulfatase synthesis was tested with several strains of Serratia. Serratia marcescens could not utilize some sugars, such as xylose, rhamnose, glucosamine and arabinose hut glucose and mannitol as a sole carbon source. However, arylsulfatase synthesis was repressed by glucose but not by mannitol. The enzyme synthesis was repressed ob inorganic sulfate and methionine, and this repression was relieved by addition of tyramine. Arylsulfatase of S. marcescen was purified by fractionation with ammonium sulfate and followed by chromatographies on DEAE-Cellulose CM-Cellulose, and DEAE-Sephadex A-25. The molecular weight of arylsulfatase was determined to be 46,000 by SDS-Gel electrophoresis and 49,000 by Sephadex G-100 column chromatography. The enzyme showed some different properties with that of K. aerogenes. The activity was maximum at pH 6.8. The Km and Vmax values for p-nitrophenyl sulfate were 2.5
－4/ M and 20 nmoles/min/mg protein, respectively. The enzyme showed high activities toward phenyl sulfate, ο-and p-nitro phenyl sulfates, and p-nitrocatechol sulfate. The inhibition of enzyme was strongly affected by hydroxylamine, inorganic fluoride, sulfide and phosphate, but by inorganic sulfate. Like Klebsiella arylsulfatase, tyramine, octopamine, and dopamine gave signifcant inhibitory effect.
Electron Microscopic Study on the Changes of the Epithelial Cells of Proximal Convolted Tubule due to Overdosage of Vitamin Complex
Microbiology and Biotechnology Letters, volume 5, issue 4, 1977, Pages 185~189
After administering overdosage of vitamin A, D ＆ E to the matured female chicken, the changes of the epithelial cells of the proximal convoluted tubule were observed and the results were summarized at follows. In the group treated with 2.5 ml of Rovisol, the mitochondria were slightly swollen and free ribosomes were mildly increased. The mitochondria were more swollen, microvilli were somewhat irregular and fused chondriosphere were noted when the chicken were treated with 7.5 ml of Rovisol. When the thicken were treated with 12.5ml of Rovisol the cell sap was markedly edematous so that the cellular organelles seemed to reduce. Also noted were chondriospgere and increased free ribosomes. According to the results of this study it is considered that the changes of the epithelial cells of proximal convoluted tubules of tile matured hens after administering overdosage of vitamin complex are mainly cellular swelling and are reversible ones.