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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 6, Issue 4 - Dec 1978
Volume 6, Issue 3 - Sep 1978
Volume 6, Issue 2 - Jun 1978
Volume 6, Issue 1 - Mar 1978
Selecting the target year
Studies on the Production and Properties of Chitinase Produced by Streptomyces sp.
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 149~153
A strain of Streptomyces sp producing chitinase was isolated from soil and its cultural condition and some properties of this enzyme were investigated. When 0.375 per cent of glucose was added to basal medium, this organism produced the most quantities of this enzyme after shaking culture at 3
for 48 hrs., while the production of the enzyme was repressed at the more concentration of glucose than that. The enzyme had a optimal pH of 7.0, optimal temperature of 5
and the activity of that was not decreased by heat treatment for 20 minute at 7
. And then the activity was increased by Co
but was slightly inhibited by Hg
Studies on the Utilization of Phenolic Substance by Yeast
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 155~159
Phenol utilizing yeast No. 558 isolated from soil sewage sediment was able to use substantial amount of phenol as the sole carbon source, and the biomass productivity by this organism was very excellent. This organism could grow well in 1000 ppm of phenol concentration, the maxim-um specific growth rate obtainable at pH 5.0, 3
was 0.27/hr., and the biomass yield coefficient Y vs. consumed phenol was 3.2. Maximum production rate of biomass was observed at 35
, pH 3.5 to pH 4.5, and the addition of the 0.005~0. 01% yeast extract was the most effective. Addition of HgCl
and phenyl hydrazine, inhibitors of oxide-reductase, in the phenol containing cultural liquid caused this organism no-growth at the concentration of 10
M respectively. This organism could utilize not only phenol but catechol, resorcinol and benzidine.
Studies on the Citric Acid Fermentation (Part 1) Strain Screening and Medium Improvement
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 161~165
Out of 11 organic acid producing strains isolated from fruits, soil, and air, one strain was selected for the study of the citric acid fermentation using Sakaguchi's medium. The organism was identified as Aspergillus niger. When Asp.niger was shaked at 3
in a cotton plugged 500 mι Erlenmeyer flask with 100 ml of Sakagnchi's medium containing 10% of glucose (Difco), 0.6% of peptone, and mineral, citric acid were produced at the level of 17 gram per liter in 14 days. The citric acid was also produced at the level of 35 gram per liter after the improvements of Sakaguchi's medium-the adaptation, peptone addition, aeration, methanol addition, and glucose addition.
Studies on the Citric Acid Fermentation (Part 2) The Citric Acid Fermentation by Asp. niger, as the Substrate of Local Commercial Glucose
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 167~171
When Asp. niger was shaked at 3
in 500 mι Erlenmeyer flask with 50 ml of the medium containing 14% of Korean local commercial glucose brand, 0.45% of peptone, and mineral, the citric acid was produced at the level of 37~43 gram per liter in 8 days, and the citric acid production at medium containing X glucose brand was better than that containing Y glucose brand. When the contaminated minerals were removed from the local glucose by Ambelite-IR 120 and peptone by potassium ferrocyanide followed by readjustment of ferric ion content in the medium to 10 mg per liter, the citric acid formation reached 53 gram per liter, a production level of three times higher than that with the original Sakaguchi's medium. The further physiological studies and the mutation of isolated Asp. niger will be needed.
Studies on the Production of Microbial Cell Protein from Hydrocarbon
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 173~179
1) To study the productivity of single cell protein from the n-paraffin utilizing yeast, 235 yeast strains were isolatea from 90 samples 2) Optimum cell growth temperature of three strains selected was 40~45
and these were identified as Candida tropicalis, Candida krusei and Torulopsis molischiana. 3) A-28 strain easily assimilated tetradecane, hexadecane and octadecane, but B-8 strain and C-15 strain assimilated more hexadecane than other n-paraffins. 4) Out of the selected three strains, the mass doubling time, specific growth rate and cell yield were 3.4~4.0 hours, 0.170~0.215, 86~98%, respectively. 5) Crude protein, fat, fiber, ash and nitrogen free extract of the selected three strains were found to be 48.2~61.2% 3.7~8.0%, 3.5~4.2%, 5.6~6.7%, 23.5~31.8%, respectively, and thiamine and riboflavin contents of dried yeast cell were 0.78~0.93 mg% and 6.03~7.3 mg%, respectively. 6) Yeast protein contained evenly most of amino acid, but the sulfur-containing amino acids were particularly low.
Studies on the Quick Fermentation of Kochujang (Part 1) Changes of Composition
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 181~185
The competitions were made between two kinds of Kochujang, one is the native fermented Kochujang which is fermented at 20
, and the other is quick fermented Kochujang which is maintained at 35
for 30 days. The results were showed that the general compositions, amino acid, nitrogen and flavor of quick fermented Kochujang were superior to the native fermented Kochujang.
Studies on the New Antimetabolites Produced by Microorganisms
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 187~196
Antimetabolite N-2292 substance, an antagonist of L-aspartic acid and L-glutamic acid was isolated from the fermentation broth of Streptomyces. Taxonomical study on the producing strain made it a related species of Streptomyces albulus judged by cultural characteristics and carbon utilization. N-2292 substance was isolated as amorphous white powder with melting point at 185
. From the physicochemical characteristics of the substance, it was peptide like substance. It was active against Gram positive and Gram negative bacteria but negative against yeast and mold in its biological properties. It was reversed by L-Asp and L-Glu on the synthetic medium.
Properties of Cellulose as an Enzyme Substrate
Kim, B. Hong ;
Microbiology and Biotechnology Letters, volume 6, issue 4, 1978, Pages 197~203
Voluminous studies have been carried out on the utilization of cellulosic materials as a raw material for the production of sugar or single cell protein. The structural complexity of the substrate generated problems in the research works as well as in the application of the results obtained in the researches. This review outlines the physical ana chemical properties of cellulosic materials obtained from researches in the field other them biology.