Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 7, Issue 4 - Dec 1979
Volume 7, Issue 3 - Sep 1979
Volume 7, Issue 2 - Jun 1979
Volume 7, Issue 1 - Mar 1979
Selecting the target year
Studies on the Immobilization of Enzymes and Microoganism Part 1. Immobilizing Method of Glucose Oxidase by Gamma Radiation
Kim, Sung-Kih ;
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 1~8
A new method for immobilization of glucose oxidate by the aerobic gamma radiation of synthetic monomers was developed. The radiocopolymerization was conducted aerobically at -70 to -8
with the mixture of several polyfunctional esters, acrylates and native enzyme. The retained activity of immobilized glucose oxidase was about 50 to 55％ when a NK 23G ester, acrylamide-bis and water mixture (1:1:2) in cold toluene treated with 450 krad of gam-ma radiation. The radiation dose did not influence significantly to the enzyme activity. The solvents used to prepare the beads of glucose oxidase and monomers were toluene, n-hexane, petoleum ether and chloroform. 0.05M tris-gycerol (pH 7.0) was a more suitable bugger solution for immobilizing the enzyme than was 0.02M phosphate. Immobilization of glucose oxidase shifted the optimum pH for its reaction from 6.0 to 6.5. The pH profile for the immobilized enzyme showed a broad range of optimum activity while the native enzyme gave a sharp pick for its optimum pH value. The immobilized enzyme reaction temperature was at the range of 30~4
Studies on the Utilization of Straw for Production of Glucose Isomerase
Han, Youn-Woo ; W. P. Chen ;
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 9~14
The hemicellulose fraction of ryegrass straw was extracted with NaOH and used for production of glucose isomerase by Streptomyces flavogriseus. Up to 25％ crude hemicellulose (20％ as pentosan) could he obtained by treating straw with 1 to 24％ NaOH. The yield of hemicellulose was increased proportionately with increasing NaOH concentration up to 4％, but the rate of increase was slowed thereafter. The optimum condition for hemicellulose extraction from ryegrass straw was to treat straw with 4％ NaOH for 3hrs at 9
or 24hrs at 3
. Highest level of glucose isomerase activity (3.04 units/ml culture) was obtained when the organism was grown for 2 days at 3
on 2％ straw hemicellulose. The organism also produced a good quantity of glucose isomerase on xylan, xylose or H
-hydrolysate of straw. The hemicellulose-extracted straw residue could be used as animal feed, because the residue had 75％ higher digestibility and 20％ better feed efficiency for weanling meadow voles than the untreated straw.
The Quantitative Change of Chitin as a Criterion to Indicate Fungal Invasion to Rice Storage
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 15~21
In order to find out a reliable chemical indicator which can be used to tell whether rice, during storage, is invaded by fungi or not, 90 percent milled rice (Tongil) was stored at 26
for 4 weeks and weekly analyzed for changes in several chemical components. The results were as follows : 1) Of several genera of fungi (Aspergillus sp., Penicillium sp., and Rhizopus sp.) observed in stored rice after surface disinfection, Aspergillus sp. were the most frequent fungi. 2) Chitin content in the rice was 25 ug/g at the beginning of the experiment and increased proportionly with moisture content of the storedrice to become 1980 ug/g at 26.9％ moisture after 4 weeks. 3) Non-reducing sugartended to decrease with increasing moisture content and or prolonged period. 4) Fat acidity showed a tendency of increase with rise in moisture content of the stared rice. Consequently, changes in chitin content can be mployed as a reliable measure to evaluate the quality of stored rice in connection with fungal invasion.
Studies on the Red Pigment Produced by Monascus sp. in Submerged Culture. Part I Isolation of Strain and Cultural Conditions of Pigment Produced
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 23~30
Fungi which produce red pigment were isolated from tapioca chips, Korean Koji, air, and plant leaves. Among the Fungi, T-1 strain was selected as test strain. This strain was identified as a Monatcus sp. by the morphological propeties. Various culture conditions, and physical and physiological characteristics of red pigment were studied. According to the studies of culture conditions, optimum condition was found to be pH 6.5; 4 days of incubation; temperature, 32~33 c: 3.5％ of Tapioca chips powder as carbon source, 0.2％ of sodium nitrate as nitrogen source and 100 ml of medium in the 500 ml Erlenmyer flask at a rotary shaker (rpm180) as aeration condition. Also effective levels of vitamins, amino acids and inorganic compounds was found to be l
/ml of folic acid and niacin; 0.3％ of L-arginine, L-glutamic acid and L-proline; and 0.001％ of manganese dioxide giving good results.
Studies on the Red Pigment Produced by Monascus sp. in Submerged Culture. Part II Production of Crude Pigment, Physical and Physiological Characteristics.
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 31~36
Yellow pigment was extracted by petroleum ether, and red pigment by 60％ ethanol. By thin layer chromatography red pigment preparation consists of more than five species whereas yellow pigment preparation consist of single species. The absorption curve of pigment solution exhibits maximum peak at wavelongth range of 495~500 nm and endo pigment at 394~403 nm. Pigment solution was relatively stable at the pH range of 3~9. Extracted pigment solution gave negative hemolysis test and pigment showed no bio-activity and nontoxicity.
Studies on the Compositional Change of Media During Oyster Muchroom Cultivation
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 37~46
In order to investigate the compositional change of the lauan and pine sawdust media during the coltivation of oyster mushroom (Pleurotus ostreatus) chemical components of the two media and mushroom were analized periodically from spawning to ending. The results are summerized as follows: 1) The total yield of mushroom from the lauan sawdust media was 6.39 kg/
, and from the Pine saw-dust media 6.31 kg/
, 94％ of which was produced from the first and second cropping period. 2) In both media ash content increased gradually, while organic matter content decreased as the cultivation progressed. Moisture content, which was about 79％ at the time of spawning, decreased a little during the priod of mycelial propagation, after which no change was observed. 3) Total nitrogen content of the two media decreased gradually as the cultivation progressed, and total loss of insoluble nitrogen was greater than that of soluble nitrogen. Amino nitrogen content tended to increase continually. 4) In both media pentosan,
-cellulose, lignin and C/N ratio decreased gradually, while the content of reduced sugar, trehalose and mannitol continued to increase. 5) In both media phosphorus, potassium, manganese and zinc decreased, and magnecium, calcium, iron and copper showed irregular changes. 6) CMC-saccharifying and liquefying activity gradually increased from after mycelial propagation to the second cropping, after which it decreased in both media. Xylanase activity rapidly and greatly increased during the second cropping period rather than the first period. At the start of the third cropping period the activity decreased slowly. Protease activity was highest after mycelial propagati, after which it gradually decreased. The pH of the two media decreased as the cultivation progressed. 7) In both media little change was observed in the content of the components of mushroom cropped from the first to the third period, but slight decrease was noticed at the fourth cropping.
Increasing the Cell Density in SCP Production from Cellulosic Substrtaes
Han, Youn W. ;
Microbiology and Biotechnology Letters, volume 7, issue 1, 1979, Pages 47~49
Undigested cellulosic substrate was frequently replaced with new substrate during fermentation. About 3-fold increase in cell density could be obtained by changing the substrate.