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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 8, Issue 4 - Dec 1980
Volume 8, Issue 3 - Sep 1980
Volume 8, Issue 2 - Jun 1980
Volume 8, Issue 1 - Mar 1980
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Microbiological Studies on the Rice Makkulli (Part 2) Nucleic Acid Degrading Enzymes and Their Related Substances during Rice Makkulli Koji Making
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 1~8
Changes of nucleic acid related substances and their enzymes during rice makkulli koji making were observed and enzymological properties of crude enzymes were examined. The results obtained were as follows : (1) The amounst of acid soluble phosphorus were increased, while no remarkable changes were observed in the component of total phosphorus during koji making. (2) AMP and IMP were increased, while ADP and ATP were decreased gradually in the course of process. (3) Activities of nucleic acid degrading enzymes were increased with the lapse of time. (4) In the crude enzyme solution extracted from rice makkulli koji, the optimal pH of RNase was 4.0~5.0 and those of PDase PNase were 5.0. (5) RNase and PMase were stable at the range of pH 4.0~5.0 and PDase was stable at the pH 4.0. (6) The optimal temperature of RNase was 55
, and that of PDase was at the range of 50~55
, and 5
for PMase. (7) Among the three enzymes, the heat stability was in order RNase, PDase and PMase, and especially PMase was so heat labile that it was almost inactivated at 7
for 10 min. (8) Inhibition by metal ions and other inhibitors was disclosed : C
inhibited the activity of RNase, and C
, NaF and N
inhibited that of PDase, while C
and NaF inhibited the PMase activity.ctivity.
RNA Production from the Hydrolyzate of Sliced and Dried Sweet Potatoes.
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 9~18
During an extensive screening tests of yeasts for their RNA formation, it was found that Cryptococcus laurentii had especially high RNA content and high dry cell weight, when hydrolyzate of sliced and dried sweet potatoes was used as a carbon source. Growth conditions of this strain were examined, and the most desirable results were obtained at 48 hours of cultivation on a reciprocal shaker at 3
with initial pH 6.0. Under the above conditions, the RNA content and yield of dry cells were investigated using various media compositions. Ammonium sulfate 0.40%, peptone 0.6 ％, and yeast extract 0.4% were appeared to be favorable as a nitrogen sources. The optimum concentrations of K
were 0.05 ％, 0.1 ％, and 0.001 ％, respectively. Ca-pantothenate, 400
, showed relatively favorable effects as a growth factor. The maximum RNA content obtained in this study was 16.8 ％ of the total dry cell weight.t.t.
Effect of Freezing and Lyophilization on Lactic Starter Cell
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 19~25
Trials of investigating the effect of freezing and lyophilization, as the practical lactic starter preservation methods, on the viability and lactic acid producing activity of Lactobacillus bulgaricus NLS-4 have been carried out. After the treatments, both of viability and activity were decreased. However, when the initial cell cocentration was increared, the survival rate against freezing could be raised to 46% and the activity to 0.25% lactic acid whereas those against lyophilization were 22 ％ and 0.29% lactic acid, respectively. There were further increased maximally when the cell suspension was subjected to freezing and lyophilization after the addition of protective agents such as glycerol and the G. C. G. S. suspending medium.
Alcohol Dehydrogenase Active on Furfuryl Alcohol from Pseudomonas sp. (Part 1) Purification and Properties of Alcohol Dehydrogenase
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 27~32
We isolated a strain of Pseudomonas sp. from soil to utilize furfuryl alcohol as a carton source by enrichment culture. Alcohol dehydrogenase from this bacteria was purified 700-fold by Sephadex G-200 and affinity column chromatography to be homogeneous by electrophoresis and analytical centrifugation. This enzyme had a molecular weight of 120,000 and was composed of four subunits consisting of 266 amino acid residues. The optimal pH of the enzyme was pH 8.5 to 9, and the optimal temperature was, 45
. This enzyme was stable at 55
, but lost 80% of its activity in 10min at 6
Studies on the Penicillin Acylase Production of Genus Escherichia
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 33~39
In order to invertigate penicillin acylase produced by a strain of Genus Escherichia, 47 strains of Genus Escherichia were isolated and examined to the extend of the inactivation of penicillins and the ability of 6-amino penicillanic acid (6-APA) production. Among them, 12 strains were found to produce penicillin acylase and to form 6-APA. The strain No. 11 of the isolates was selected to be the most excellent one producing the acylase. Optimum culture conditions for the production of the acylase of the strain were found as follows : pH at 7.6~8.0, time on 18 hrs, temperature at 34 to 38
. And effective levels of the medium were found to be contained 0.3% phenylacetic acid, 1.0％ yeast extract, 1.0％ peptone and 0.3％ L-glutamate. And, the production of the acylase by the isolate was strongly inhibited by 1% glycerol and the growth was remarkably retarded by the addition of 1.0% n-butylacetate. The acylase was extracted from the isolate and the crude enzyme of the acylase was prepared and the characteristies of the enzyme were primary examined in optimum pH, temperature, stabilities and reaction time.
Reaction Kinetics and Absorption Property of Low Molecular Weight Endo-glucanase Component of Cellulase
W.S. Ryu ; Dewey D. Y. Ryu ;
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 41~46
Low molecular weight endo-glucanase was partially purified from cellulase complex using Sephadex G-100 gel chromatography. Biochemical properties of the purified component was investigated. Optimum pH and temperature determined were 6.0 and 5
, respectively. Enzymatic hydrolysis of four cellulosic substrates having varying crystallinity was evaluated. It was found that hydrolysis of amorphous region was followed by the hydrolysis of crystalline region. In order to examine the effect of adsorption of the enzyme onto the cellulosic substrates on the hydrolysis kinetics, adsorption studies were carried out. Time course of adsorption of low molecular weight endo-glucanase onto various cellulostances was observed for 25 min. The rate and amount of adsorption to amorphous cellulose was greater than those to the crystalline cellulose. This result suggested that the role of endo-glucanasc was more important to the hydrolysis of amorphous cellulose than to the crystalline region of the cellulose.
Studies on the Citric Acid Fermentation with Fungi (Part I) Isolation and Identification of Strains
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 47~53
For the Purpose of studies on the citric acid fermentation, 579 strains of Aspergilli were isolated from natural sources of microorganisms. Out of them, the strains of M-80 and M-315 which produced relatively larger amount of citric acid than any others were selected after calling out an extensive screening test. The results obtained in light of the manual of Raper had been shown that the selected strains of M-80 and M-315 were identified as Aspergillus usamii mut. shirousamii, Aspergillus ficuum, respectively.
Higher Biomass Production of Lactobacillus bulgaricus NLS-4 by Improvement of Cultural Conditions
Rhee, Sang-Ki ; Pack, Moo-Young ;
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 55~60
Some of the cultural conditions were improved in order to obtain the higher biomass of Lactobacillus bulgaricus NLS-4 which has the higher lactic acid producing activity as well. Among eight media including 11％ non-fat milk medium as a control, the TIP medium was selected. By a batch experiment, the maximum cell concentration could be increased to 1.0
when the organism was grown at 38
for 18 hours with agitation speea of 200 rpm and under the constant level of pH 6.5 con-trolled with 1 N KOH solution in the selected medium. The cell concentration was further increased to 2.3
cells per me in the steady state of continuous culture at the dilution rate of 0.17 hr
for 18 hours.
Studies on the Night Soil Treatment
Ose, Youki ; Ishikawa, Tetsuya ; Sato, Takahiko ;
Microbiology and Biotechnology Letters, volume 8, issue 1, 1980, Pages 61~73