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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 8, Issue 4 - Dec 1980
Volume 8, Issue 3 - Sep 1980
Volume 8, Issue 2 - Jun 1980
Volume 8, Issue 1 - Mar 1980
Selecting the target year
Studies on the Production of Protease by Aspergillus oryzae KC-15 and Characteristics of the Enzymes
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 77~85
This experiment was conducted to investigate the conditions for production and the characteristics of pretenses. Aspergillus oryzae KC-15, which is selected as a superior strain for the production of the protease, was used in this study. The results obtained were as follows: 1. The optimum culture time for the production of acid, neutral and alkaline protease on wheat bran medium were about 48, 48 and 72hr, respectively. The protease-produced by the strain were mainly alkaline and neutral one, but the production of acid protease was feeble extremely. 2. The addition of NaH
, glucose, rice powder and Na-glutamate respectively to wheat bran media were effective for the production of alkaline and neutral protease, and the addition of (NH
, glucose and rice powder respectively were effective for the production of acid protease. 3. Characteristics of professes(equation omitted) 4. As a heat resistance agent, NaH
was the most effective one. The optimum amount of NaH
was 10mg for alkaline and neutral protease, and 5mg for acid protease. 5. The heat resistance of the Protease by NaH
was not recognized mostly above 6
. 6. After the treatment of enzyme solution with 10mg of NaH
for 30 minutes at 55
, the residual activities measured for alkaline, neutral and acid protease were 58, 57 and 55％ respectively.
Studies on the Citric Acid Fermentation by Fungi (Part II) Preservation of the Selected Strains and the Effect of UV-Irradiation
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 87~92
These experiments were conducted to manage more safe the selected strains and improve their characteristics. The results obtained were as follows: Preservation on soil at the range of 0 to 5 C was suitable and there were no remarkable changes on their abilities to produce citric acid until 10 months preservation. The successive transfer of spore slightly stimulated the selected strain, M-315 to produce citric acid and the spore precultured on sporulation medium for 7-10 days was desirable as inoculum. Under UV-irradiation from the selected strains, 109 mutants whose morphological characteristics were changed were isolated. Among them, the mutant M-80-12 was shown 3.2％ increase on acid producing ability than that of its parent.
Studies on Conditions of the Gluconic Acid Production by a Mold isolated from the Soil of Seoul Area
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 93~102
Fungi which were capable of producing gluconic acid were isolated from soil and tree leave samples, which had been collected in Seoul ana its vicinity. Among the 19 strains isolated, a strain named arbitrarily KUF-O4 was selected as a test strain chiefty because of its efficiency in gluconic acid production. The strain was identified as an Aspergillus sp. through its morphological properties. Optimum conditions for the gluconic acid production of KUF-O4 were investigated. The results obtained are as follows. 1. An incubation period of at least 30 hours was required for a good yield of gluconic acid. 2. A medium containing 10％ glucose needed at least 3 ％ CaCo
to maintain the optimum pH for the production of gluconic acid during fermentation. 3. As a carbon source, glucose was the most effective one among the carbon sources tested. 4. As a nitrogen source, an ammonium salt was more effective than any other form of nitrogen compounds. 5. As mineral source, a small amount of both KH
was found to be necessary to increase the efficiency of the gluconic acid production.
The Manufacture of Vinegar from Fallen Persimmons
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 103~111
This studies were conducted to make vinegar from the physiologcical fallen persinmon during the ripening fruit. The main components of the persimmon mere investigated for the several focal valieties, Some microke acted on the fermentation of persimmon was isolated. The obtained results were as follows : 1) The hardness of fruit was the hithest for Jangjunsi (long persimmon) The hardness of Bansi and Soosi valieties was decreased vary fast after five or six weeks. 2) The total amount of pectin was increased a little in order of Jangjunsi, Bansi and Soosi. The amount of soluble pectin was increased considerably in order of Soosi, Bansi and Jangjunsi. 3) The amount of total sugar and reducing sugar were increased in order of Bansi, Jangjunsi and Soosi. After five or six weeks the amount of reducing sugar of Soosi and Bansi was increased much. The amount of starch was decreased in order of Bansi, Jangjunsi and Soosi, to trace amounts without significent differences. 4) The amount of Soluble tannin was decreased in order of Bansi, Jangjunsi and Soosi. 5) Main microbes on the fermentation of persimmon vinegar were identified as follows: yeast was proved to be Saccharomyces rouxii, CBS 726, and Acetobacters were Gluconobacter oxydans subsp. suboxydans, Gluconobacter oxyaans subsp, osydans, Acetobaeter pasteurians subsp. xylinum 6) During the fermentation, process of persimmon vinegar the amount of reducing sugar and alcohol were decresed, but that of acidity was increased.
The effect of the divalant Metal ions on the ATPase activity in Myofibrillar protein of the Muscle of Rabbit fed Vegetable Oils.
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 113~117
The effects of divalant metal ions on the ATPase activity were studied by using my of ibrillar protein of rabbit (Chin-Chilla species) fed with vegetable oils. The results obtained were as follows : 1. The ATPase activity in myofibrillar protein of the is not significant to Rabbit exhibited a common biohapsic respnse, such as the ATPase activity is high at a lower ionic strength and low at a higher ionic strength. 2. The effect of EDTA on the ATPase activity of Myofibrillar protein extracted from Rabbit fed vegetable oils was tested by using various concentrations. The ATPase activity was inhibited from 0.2mM and over concentration of EDTA. 3. The ATPase activity in Myofibrillar protein was decreased remarkably in 0.2mM and over concentration for
, and in 1.0mM and over concentration for
. 4. in vitro, the digestibilities in A, B, C and D groups of Rabbit muscle treated with Papsin and Trypsin for 30 minutes at 36
water bath were 71.66％, 73.87％ ； 70.62％, 77.93％ ； 67.93％, 76.52％ ； and 86.79％, 90.22%, respectively.y.
Studies on the yellow pigment produced by Monascus sp. CS-2 PartI. cultural conditions for yellow pigment produceduction.
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 119~123
Culture conditions of yellow pigment in Monascus sp. were studied. According to the studies of culture conditions optimum condition was found to be pH 4.5, 3 days of incubation with 3% of sucrose as carbon source, 0.2 ％ of yeast extract as nitrogen source and 75m1 of medium in the 500m1 erlenmyer flask by rotary shaking (rpm 180) at 180 r.p.m. Effective levels of inorganic compounds were found to be 0.25 % of potassium phosphate monobasic and 0.1 ％ of Magnesium sulfate.
Identification of a Bacterium which Produced D-Glucose Isomerase and Partial Purification on the Enzyme
Rhee, In-Koo ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 125~133
A microorganism which produced D-glucose isomerase was identified to be similar to Streptomyces antibioticus on the morphological, cultural and physiological characteristics except the spore chain and the utilization of sucrose. D-xylose grown cells of Streptomyces sp. strain K-17 were disrupted by grinding with sea sand. D-glucose isomerase was partially purified with the fractionation by ammonium sulfate, Mn-treatment, DEAE-cellulose column chromatography, DEAE-sephadex (A-50) column chromatography and gel filtration of sephadex G-200. The enzyme was purified about 380 fold with 25 ％ recovery.
Wastewater Treatment by Microorganism
Microbiology and Biotechnology Letters, volume 8, issue 2, 1980, Pages 135~142
The process of biological treatment of organic wastewater is principally associated with those of self-purification in the natural water environment. The treatment system has e intensive function of stabilizing wastewater more effectively than in natural water, which is like natural water concentrated in a small space. Biological treatment of wastewater involves activated sludge and various modified process, trickling filter, rotating disk, oxidation ditch, etc. for aerobic decomposition and anaerobic processes such as anaerobic decomposition and methane fermentation. The basic characteristic of these processes is the use of mixed culture for the conversion of pollutants. This review forcuses on the various kinds of microorganisms related to each treatment processes. Kinetic analysis of the activated sludge process is discussed in order to understand the basis of control and maintenance of the biological treatment process.