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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 9, Issue 4 - Dec 1981
Volume 9, Issue 3 - Sep 1981
Volume 9, Issue 2 - Jun 1981
Volume 9, Issue 1 - Mar 1981
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Studies on the Production of Guanosine-5'-monophosphate by Microorganism. (Part III) Studies on the Crystallization of 5'-GMP. 2Na.
Microbiology and Biotechnology Letters, volume 9, issue 1, 1981, Pages 1~6
Crystallization conditions of disodium guanosine-5'-monophosphate (5'-GMP. 2Na) were studied. The solubility of 5'-GMP. 2Na was decreased by addition of methanol and the optimum condition was as follows. The crystallization was carried out at 45
with agitation rate of 160-200 rpm., which is Reynold's No. of 25, 000-32, 000. When concentration of methanol was 7.5%~10.0%, the 5'-GMP. 2Na was easily crystallized by addition of crystal seed.
Studies on the Production of Gluconic Acid by Resting Cell System of Aspergillus niger
Microbiology and Biotechnology Letters, volume 9, issue 1, 1981, Pages 7~19
The production of gluconic acid from glucose by the resting cell system of Aspergillus niger was studied. It was found that the conversion products from glucose by the resting cell system were markedly influenced by the pH, temperature, substrate concentration, aeration, metal ions, cultivation time and storage conditions of the resting cells. Conversion products were identified as gluconic acid by the thin layer chromatography and infrared spectrophotometry. These conversions were greatly stimulated by addition of
, and S
, but showed inhibitory effects by C
and cyanide. For the optimum cell storage, it was effective to be kept at -
in 0.05M phosphate buffer solution of pH 7.0. The gluconic acid production by the resting cell system was more effective than those of the fermentation with respect to cultivation time, yield, recovery and re-use of the cell.l.l.l.l.l.l.
Antioxidant properties of red-pepper peel extracts on margarine.
Microbiology and Biotechnology Letters, volume 9, issue 1, 1981, Pages 21~27
The effects of anti-oxidant of ether extract of red pepper peel were studied to compare with butylated hydroxyl toluene (BHT), citric acid and ascorbic acid. Peroxide and acid values of magarine irradiated with the sunlight were detected. The results were as follows: 1. The extract of red pepper peel influenced as antioxidant of magarine. But the extract was lower anti-oxidative effect than that of BHT at the same concentrations. The anti-oxidative effect increased with the concentration of the extract applied. 2. There was no synergistic and anti-oxidative effects, when the extract was used together citric and ascorbic acids. 3. The peroxide value of fermented magarine was controlled by the addition of the extract and BHT. However acid value of it was not controlled as much as peroxide value.
Studies on Microbial Penicillin Amidase ( I ) Optimization of the Enzyme Production from Escherichia coli
Kim, Bong-Hee ; Seong, Baik-Lin ; Mheen, Tae-Iek ; Moon H. Ban ;
Microbiology and Biotechnology Letters, volume 9, issue 1, 1981, Pages 29~34
To maximize the production of penicillin amidase from Estherichia coli (ATCC 9637), the media composition and several factors affecting the engyme production during fermentation were studied. The optimal media composition was found to be; 3.5% tryptone, 1.5% monosodium glutamate and 0.5% yeast extract. The addition of 0.15% phenylacetic acid as an enzyme inducer at the initial stage of cultivation increased the engyme productivity about 5 fold. It was found that the engyme activity reached maximum within 16hr of cultivation. The maximum production of the enzyme obtained was about 102.5 units/l broth under the optimized condition. The enzyme production was markedly increased by the optimization as compared with those previously reported.
Studies on Microbial Penicillin Amidase (II) Characteristics and the Reactor Performance of Whole Cell Immobilized Penicillin Amidase of Escherichia coli
Seong, Baik-Lin ; Kim, Bong-Hee ; Mheen, Tae-Iek ; Moon H. Han ;
Microbiology and Biotechnology Letters, volume 9, issue 1, 1981, Pages 35~44
Whole cell penicillin amidase of Escherichia coli was immobilized by entrapment in gelatin followed by extrusion and crosslinking with glutaraldehyde. The immobilized engyme preparation demonstrated the recovery yield of activity up to 70% and good stability during storage and operation. The half life of activity decay during the operation was estimated to be about 50 days. The optimum pH and temperature for both of immobilized and soluble enzyme are 8.5 and 5
, respectively. No significant change was demonstrated in the effect of pH and temperature, but the increase in heat stability at high temperature was observed in the case of the immobilized enzyme. It was found that the plug flow reactor could be operated favorably since the pH drop along the column path due to tile reaction product was minimized by employing substrate solution with moderate buffer strength. The optimal condition of reactor operation was discussed with regard to the effect of substrate concentration and the residence time on the conversion efficiency and productivity.
Studies on the Fermentative Production of Inosine 5'-monophosphate by Microorganisms. (Part II) Effects of Carbon Source and Purine Base on Inosine 5'-monophosphate Accumulation by a Mutant of Brevibacterium ammoniagenes
Microbiology and Biotechnology Letters, volume 9, issue 1, 1981, Pages 45~50
The effect of growth and the carbon sources including the molar ratio of fructose to glucose was studied for the maximization of inosine-5'-monophosphate (5'-IMP) production from Brebibacterium ammoniagenes D-21530. According to experimental results, fructose was more efficient to 5'-IMP accumulation than glucose, while the latter was better for the cell growth than the former. To synchronously use glucose and fructose as carbon source is to optimally control the cell growth and maximum production of 5'-IMP without change of other conditions. The optimal weight percent of fructose to sum of glucose and fructose was 20～40%, and the productivity improvement over the utilization of fructose was about 40%. And also the optimality of purine base such as adenine and guanine were considered. The optimal concentrations of adenine and guanine were near 50㎎/l.