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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Microbiology and Biotechnology Letters
Journal Basic Information
Journal DOI :
The Korean Society for Applied Microbiology and Biotechnology
Editor in Chief :
Volume & Issues
Volume 9, Issue 4 - Dec 1981
Volume 9, Issue 3 - Sep 1981
Volume 9, Issue 2 - Jun 1981
Volume 9, Issue 1 - Mar 1981
Selecting the target year
Production of Lactic Acid from 1,2-Propanediol by Yeast
Chae, Ki-Soo ; Seu, Jung-Hwn ;
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 51~57
1,2-propanediol-utilizing yeast, Y-1-4, was isolated from sludge sample by the enrichment culture technique. The product produced from 1,2-propanediol by the selected strain was identified as lactic acid by paper chromatography and infrared absorption spectrum. The strain assimilated ethanol, 1,2-propanediol, glycerine and glucose, but it produced lactic acid from 1,2-propanediol used as the sole carbon source. Under optimal conditions, the strain Y-1-4 was cultured with shaking at 3
for 4days in the medium containing 1,2-propanediol 20.0g, NH
Cl 5.0g, KH
0.25g, yeast extract 0.4g, CaCO
3. 0g and tap water to one liter, and then the yield of lactic acid was about 12. 1g per liter of the culture broth.
Cultural Conditions of Heavy Metal-ion Tolerant Microorganism and Accumulation of Heavy Metal-ion into the Cells.
Yu, Tae-Shick ; Song, Hyung-Ik ;
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 59~64
The cultural conditions and the intra cellular accumulation of cadmium was studied using a cadmium tolerant yeast strain B-7 which had been isolated from activated sludge collected from a zinc mining area. The organism was able to grow in a medium containing 3,000
of cadmium-ion. (C
) Optimum conditions for the growth of the organisms were 20~22
and pH 5.0~8.0 under aerobic condition. The maximum cadmium accumulation was observed when the organism was grown at pH 6.0. The growth of B-7 was not affected by the addition of a silicone-based antifoamer, which stimulated the intra cellular accumulation of cadmium. The intra cellular cadmium accumulation started after the cell ceased to grow. One gram of cells accumulated 34.17mg of cadmium when the organism was grown in a medium containing 500
of cadmium and 0.2%, v/v silicone-based antifoamer at 28
for 48 hours with shaking. About 73 % of the accumulated heavy metal by the organism was found in the cytoplasm.m.
Studies on the Microbial Utilization of Agricultural Wastes (Part 12) Comparisions of Cellulolytic Methods for Ethanol Production from Cellulosic Material
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 65~69
As a process to utilize agricultural residues, simultaneous hydrolysis-fermentation (SSF) was compared with fermentation of enzymic hydrolyzate using koji cultures of Trichoderma sp. KI 7-2 and a thermotolerant yeast Saccharomyces cerevisiae NCYC 716. Cellobiose was not detected in SSF broth whilst 15 mg/
of the disaccharide was found in enzymic hydrolysate of rice straw using the same enzyme source. It was found that converting glucose to ethanol in SSF process reactivated the cellobiase activity, which is inhibited by the accumulation of glucose in enzymic hydrolysis process. Cutting milled rice straw was fermented as effectively as ball milled one in SSF process. From tile results discussions are made on the product inhibition mechanism of cllulolytic enzyme system.
Studies on the Microbial Utilization of Agricultural Wastes (Part 13) Optimization of Simultaneous Hydrolysis-Fermentation for Ethanol Production from Rice Straw
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 71~75
Studies were made to optimize the simultaneous hydrolysis-fermentation (SSF) process for the production of ethanol from rice straw. Trichoderma sp. KI 7-2 was selected to produced cellulase by solid culture for SSF. Ethanol production was highest when the SSF process utilized koji culture of the fungus grown on a medium of wheat bran-rice straw 3 : 2 mixture with moisture content of 50% adjusted to pH 4.5 for 7 days as the enzyme source. It was found that pretreatment of the substrate is not necessary. To ferment 1g of rice straw by SSF 2.47 units of cellulase were required, and the initial yeast concentration of 2.5
was found to be sufficient. Optimum pH and temperature for the process were 4.5 and 4
, respectively. It was also found that higher ethanol concentration in the broth can be obtained by the addition of substrate or substrate and enzyme to SSF broth.
A Study on the Alcohol Distiller′s Waste Treatment by Microorganisms
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 77~82
Candide tropicalis was selected for its ability to utilize spent waste generated by the alcohol distillery using tapioca starch as a raw material. Optimum pH and temperature on batch culture of the organism were 4.0 and 3
. The growth of the organism was markedly increased when 0.2% of ammonium sulfate, 0.002% of potassium phosphate dibasic, add 0.04% of magnesium sulfate were supplemented to the filtrate. At these conditions, maximum specific growth rate and saturation constant were 1.0 hr
and 4.4 g.1
, respectively. At a dilution rate of 0.5hr
, a productivity of 1.84 g.1
was obtained and about 70% of carbohydrate was assimilated. Protein content of dried cell was about 60%.
Biomass Production and Cell Wall Lysis of Rhizopus oryzae
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 83~89
Several kinds of organic acids, alcohols, aromatic compounds and sugars as carbon sources were tested in order to produce the cell mass of Rhizopus oryzae which is used in part of food processing or organic acid fermentation. Sodium acetate among them was good enough for carbon source as well as glucose under the concentration of one percent. All nitrogenous substances tested such as ammonium, nitrate or organic nitrogen compounds were well used by this strain of Rhizopus oryzae as nitrogen source. Ammonium sulfate among inorganic nitrogen compounds was most utilized as a nitrogen source in glucose or acetate medium. This strain did not require any growth factors such as yeast extract. The following composition of medium was therefore determined in order to produce the cell mass of Rhizopus oryzae: Na-acetate 1 %, (NH
0.01%, NaCl 0.01% (PH 5.5). The cell wall of mycelium grown in above medium was lysed optimally at pH 6.5 and 5
by the action of Strepzyme 115-5. On producing protoplast from mycelium by enzymatic action, almost all of the mycelium was damaged after 4hrs of treatment.
Studies on the Production of Thermostable Amylase. Part 1. Optimal Culture Conditions and Purification of Enzyme.
Microbiology and Biotechnology Letters, volume 9, issue 2, 1981, Pages 91~97
A thermophilic soil isolate Bacillus sp. Y-127 was selected for the production of thermostable amylase. The strain was used for the enzyme production and the thermostable amylase was characterized. The optimum cultural conditions for the enzyme production were 6
at pH 7.0 for 32 hours using a mineral medium containing 2% soluble starch and 0.2% yeast extract. The extra-cellular enzyme was purified about 123-folds with about 6% recovery. The purified enzyme was stable at pH between 4.0 and 7.0, and temperature up to 6