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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
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Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 13, Issue 4 - Dec 2009
Volume 13, Issue 3 - Sep 2009
Volume 13, Issue 2 - Jun 2009
Volume 13, Issue 1 - Mar 2009
Selecting the target year
Rainfastness of Two Fungicides Tank-mixed with Spreader-sticker
Choi, Yun-Kyong ; Yu, Ju-Hyun ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 203~208
In order to elucidate the effect of spreader-stickers on the rainfastness of dithianon and chlorothalonil wettable powders, and to estimate the possibility of suggesting good new formulations, the fungicide residues on the leaf surface of hot pepper was assessed and compared after the drop-wise applications of fungicide suspensions containing spreader-sticker on leaf surface followed by artificial raining. Three commercial spreader-stickers, which were Cover, Reitron and Silwet, not only made the rainfastness of dithianon wettable powders worse on hot pepper leaf, but increasing their concentrations also accelerated it further. On the other hand, to chlorothalonil wettable powder, Reitron showed the 3-fold improvement of rainfastness. But, for the rest, there was no improvement as well. The effect of N-octylpyrrolidone (NOP) on rainfastness of both fungicides was excellent. Soybean oil formulations containing leaf-penetrable nonionic surfactant, which was either polyoxyethylene monotridecyl ether or polyoxyethylene monolauryl ether, improved dithianon rainfastness, but the ones containing conventional emulsifiers did not.
Studies on the Processing Factors of Pesticide in Dried Carrot from Field Trial and Dipping Test
Park, Kun-Sang ; Suh, Jung-Hyuck ; Choi, Jeong-Heui ; Kim, Sun-Gu ; Lee, Hyo-Ku ; Shim, Jae-Han ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 209~215
This study was performed to produce the processing factors of pesticides in dried carrot. It is essential data for establishing the maximum residue limits (MRLs) of pesticides in dried carrot. The target pesticides were azinphos-methyl, chlorpyrifos, captan, endosulfan and triclorfon. These pesticides are included Korea's MRL list in carrot and USA's MRL list in dried foods. To infiltrate these pesticides up to each MRL level in carrot, the dipping test was performed in laboratory. Also, the supervised residue trial of the pesticide for carrot was conducted in the green house to recognize the field trial's tendency. In the dipping test in laboratory (including drying examination), the processing factors of the carrot at various concentrations and temperatures could be evaluated. In field test, the processing factors were 5.9 for azinphos-methyl, 1.7 for captan, 7.6 for chlorpyrifos, 6 for endosulfan, 0 for trichlorfon, respectively. The dipping test in laboratory on various kinds of conditions showed more precise processing factors than field trial. The processing factors obtained from the dipping test of carrot were 0~4.7 at the various concentration of the pesticides, and 0~6.7 at various drying temperature. The lower level processing factors were 0~0.6 for trichlorfon and the higher level were 3.0~5.8 for chlorpyrifos. The highest processing factor was 9.1 for captan.
Monitoring of Pesticide Residues in Floricultural Crops Collected from Floral Farms and Markets in Korea
Lee, Kyung-Hee ; Kim, Seong-Soo ; Park, Hong-Ryeol ; Ji, Kwang-Yong ; Kim, Jong-Geol ; Huh, Kun-Yang ; Hur, Jang-Hyun ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 216~222
The present study aims to monitor pesticide residues in cut flowers collected from the farms and markets. Cut flowers used in this study included rose, lily and chrysanthemum collected from June to September, 2008. Samples were collected once from farms in Hwasung, Goyang (Gyeonggi-do), Inje (Gangwon-do) and thrice from wholesale market in Namdaemunm, Yangjae and Gangnam (Seoul). Total of 24 pesticides (12 fungicides, 11 pesticides and 1 acaricide) were detected from samples collected from farm and total of 64 pesticides (25 fungicides, 36 pesticides, 1 acaricide and 2 fungicides) were detected from samples collected from wholesale market. The highest detection frequency of pesticide from farm was for carbaryl (15%) and for boscalid, fluacrypyrin, fluquinconazole, methomyl, pyraclostrobin, trifloxystrohin (10%), with overall detection of
. While the highest detection frequency of pesticides from wholesale market was for carbaryl, fluquinoconazole and kresoxim-methyl (18.52%), methomyl (16.6%), and methiocarb and thiacloprid (12.96%) with overall detection amount of
. Higher amount of pesticides were detected in leaves than in flowers. Among the pesticides detected, detection frequency of unregistered pesticides for rose, chrysanthemum and lily was 55%, 60% and 63% collected from farms and 47%, 60% and 89% collected from markets, respectively. These pesticides require registration and further monitoring in floricultural crops.
Establishment of Analytical Method for Fenhexamid Residue in Korean Cabbage, Apple, Mandarin and Green Pepper
Lee, Hye-Ri ; Riu, Myoung-Joo ; Park, Hee-Won ; Na, Ye-Rim ; Song, Hyuk-Hwan ; Keum, Young-Soo ; Zhu, Yongzhe ; Kim, Jeong-Han ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 223~231
This study was performed to develop a precise single residue analytical method of fungicide fenhexamid in representative crops for general residue analytical method which could be applied to most of crops. Korean cabbage, mandarin, apple and green pepper were selected, macerated, extracted with acetone, concentrated and partitioned with dichloromethane. Then the extracts were concentrated and cleaned-up through Florisil column with ethyl acetate/0.1% acetic acid in hexane [15:85, (v/v)] before concentration and analysis with HPLC. LOQ (Limit of Quantitation) of fenhexamid was 1 ng (S/N>10) and MQL (Method Quantitative Limit) was 0.01 mg/kg. Recoveries were measured at two fortification levels (10 MQL and 50 MQL) on crop samples and ranged from 85.2% to 94.8% (mean recoveries) and coefficients of variation were <10% regardless of sample type.
Residues of Azoxystrobin during Cultivation and Processing of Ginseng
Kim, Jong-Geol ; Kim, Seoung-Su ; Park, Hong-Ryeol ; Ji, Kwang-Young ; Lee, Kyung-Hee ; Ham, Hun-Ju ; Im, Moo-Hyeog ; Hur, Jang-Hyun ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 232~240
The aim of this study was to determine the processing and reduction factors for ginseng and its commodities during ginseng processing to obtain information of pesticide residue in ginseng. For this study, azoxystrobin was used in two field containing 6 years old ginseng plants. Ginsengs were harvested and processed to obtain different commodities (Dried ginseng, red ginseng and ginseng water and alcohol extracts, red ginseng water and alcohol extracts) for pesticide analysis. The amount of residue levels from wonju and icheon for fresh ginseng were 0.05,
dried ginseng were 0.12,
, red ginseng were both
, ginseng alcohol extract were 0.28,
, ginseng water extract were 0.22,
, red ginseng alcohol extract were 0.31,
and red ginseng water extract were 0.09,
respectively. These data were under MRLs notified by KFDA. The processing factors for ginseng products were 3.25, 1.34, 7.84, 4.63, 6.15 and 2.56 respectively. The reduction factors for ginseng products were 1.19, 0.51, 3.41, 1.91, 2.74 and 1.00 respectively. These data showed increment during processing which could be due to concentration but considering water contents, residue levels were similar or decreased than the initial residue level during processing.
Establishment of Pre-Harvest Residue Limit (PHRL) of Insecticide Bifenthrin during Cultivation of Grape
Kim, Sung-Woo ; Lee, Eun-Mi ; Lin, Yang ; Park, Hee-Won ; Lee, Hye-Ri ; Riu, Myoung-Joo ; Na, Ye-Rim ; Noh, Jae-Eok ; Keum, Young-Soo ; Song, Hyuk-Hwan ; Kim, Jeong-Han ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 241~248
Pre-Harvest Residue Limit (PHRL) of bifenthrin during cultivation of grape was established by utilizing the dissipation curve and biological half-life of bifenthrin calculated from the analysis of 0, 1, 2, 3, 5, 7, 10, 15 days after treatment of bifenthrin. Grape sample was extracted and partitioned with acetonitrile and dichloromethane, respectively, and bifenthrin was determined with GC/ECD. Limit of quantitation (LOQ) of bifenthrin was 0.01 ng. Recoveries at two fortification levels of 0.1 and
, respectively. The biological half-lives of bifenthrin were about 21 days at standard application rate, while, 23 days at double application rate. Dissipation of bifenthrin on grape was not influenced by growth dilution effect. The PHRLs of bifenthrin were recommended as 0.60 and
for 10 and 5 days before harvest, respectively.
On the Processing and Reduction Factors of Several Pesticides with Welsh Onion
Park, So-Yeon ; Kang, Hye-Rim ; Ko, Kang-Young ; Gil, Keun-Hwan ; Im, Moo-Hyeog ; Lee, Kyu-Seung ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 249~255
In order to calculate the processing and reduction factor between fresh and dry Welsh onion which was widely used as a dried agricultural ingredient of food in Korea. 7 pesticides such as pyridalyl, kresoxim-methyl, spinosad, flufenoxuron, difenoconazole, metconazole, and tebufenozide were tested. After 2 sites field trial conducted and measured water contents by drying at
and analyzed the pesticide residue. Water contents of fresh and dried Welsh onion are 89.2 and 10.2% respectively. Averages of processing factor showed 7.24 of pyridalyl, 2.85 of kresoxim-methyl, 7.43 of spinosad, 3.17 of flufenoxuron, 4.38 of difenoconazole, 2.40 of metconazole and 8.13 of tebufenozide into 2 field samples. Averages of Reduction factor showed 0.87 of pyridalyl, 0.35 of kresoxim-methyl, 0.88 of spinosad, 0.38 of flufenoxuron, 0.52 of difenoconazole, 0.29 of metconazole and 0.98 of tebufenozide. Residual amounts of pesticides in/on fresh Welsh onion reduced during drying process.
Comparison of Acute Toxicity of Molinate on Two Aquatic Insects, Chironomus riparius and Cloeon dipterum in Different Larval Stages
Kim, Byung-Seok ; Park, Yoen-Ki ; Hong, Soon-Sung ; Yang, Yu-Jung ; Park, Kyung-Hun ; Joeng, Mi-Hye ; Kim, Se-Ri ; Park, Kyeong-Hun ; Yeh, Wan-Hae ; Kim, Doo-Ho ; Hong, Moo-Ki ; Ahn, Young-Joon ; Shin, Jin-Sup ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 256~261
Molinate (S-ethyl hexahydro-1H-azepine-1-carbothioate), a selective, preemergence thiocarbamate herbicide used for grass weed control in rice fields, is being most widely used in Korea. This study was conducted to assess the comparative toxicity of molinate on two aquatic insects, Chironomus riparius and Cloeon dipterum in four larval stages. First-instar larvae of C. riparius appeared to be the most sensitive to acute exposure of molinate with a 48-h
of 7.8 mg/L, followed by third instar (48-h
), second instar (48-h
) and fourth instar (48-h
> 50 mg/L) larvae. Also, the sensitivity of the larvae of C. dipterum was presented as the same manner of the larvae of C. riparius. The youngest group of larvae of C. riparius appeared to be the most sensitive to molinate, with 96-h
values ranged from 14.3 to 24.1 mg/L. The authors assume that the young instar lavae of aquatic insects, Chironomus riparius and Cloeon dipterum are more sensitive to molinate. Also, the authors propose that Mayfly, Cloeon dipterum is well suited as a bioassay organism because it is one of the most vulnerable aquatic insects inhabiting agricultural ecosystem in Korea.
Canola Plant Growth Promotion by a Selected Plant Growth Promoting-Rhizobacteria, Burkholderia pyrrocinia Strain 13-1 in the Cold Condition
Lee, Jae-Eun ; Cho, Sang-Min ; Cho, Young-Eun ; Park, Kyung-Seok ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 262~266
Plant growth-promoting rhizobacteria (PGPR) are beneficial native soil bacteria that colonize plant roots and result in increased plant growth. The objective of this study was to determine the plant growth promotion in canola plants by selected PGPR strain 13-1 under low temperature condition. The seed treatment of strain 13-1 was enhanced plant height and root elongation on canola plant at low temperature condition. This result determined that a selected strain of PGPR can enhance plant growth and root propagation under extremely low temperature conditions. Thus, this PGPR strain extends their role on plant growth promotion on canola until low temperature condition for practical applications.
Selection of Trunk Injection Pesticides for Preventive of Pine Wilt Disease, Bursaphelenchus xylophilus on Japanese Black Pine (Pinus thunbergii)
Lee, Sang-Myeong ; Kim, Dong-Soo ; Lee, Sang-Gil ; Park, Nam-Chang ; Lee, Dong-Woon ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 267~274
This study was carried out to select effective preventive pesticides against pine wilt disease caused by pinewood nematode (PWN), Bursaphelenchus xylophilus on trunk injection. 1,000 fold aquatic solution of abamectin 1.8% EC and emamectin benzoate 2.15% EC were lower mortality (7.3% and 8.3% respectively) against PWN on 1 day after treatment. However effects of abamectin 1.8% EC, emamectin benzoate 2.15% EC, fosthiazate 30% SL and fenitrothion 30% SL were inhibited the reproduction of PWN over 99.6% in Botrytis cineria media. Effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of
per 10 cm in diameter of breast height (DBH) on mortality of Japanese black pine, Pinus thungergii by inoculated PWN was 0% and 3.3%, respectively at the applied year however when injection of fosthiazate 30% SL were treated with the rate of
per 10 cm tree DBH, mortality of tree was 63.3%. Abamectin 1.8% EC and emamectin benzoate 2.15% EC was showed high preventive efficacy representing >90% against PWN at the following year. PWN preventing efficacy of trunk injection was lower in naturally occurred area (mortality of pine tree in control was 11.7% at the first year) of PWN than artificially infected site (mortality of pine tree in control was >76.7% at the first year), PWN preventing efficacy of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of
per 10 cm in DBH was 91.5% and 82.9%, respectively, at the applied year and 89.5% and 82.6% respectively at the following year in PWN naturally occurred site. Control efficacy by trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC was more higher in 10 fold dilution with 10 fold high amount of aquatic solution than no dilution with 10 fold less amount of aquatic solution. The preventive effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of
per 10 cm in DBH was showed 100% at the applied year in PWN inoculated tree.
Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot
Lee, Jae-Eun ; Lee, Seo-Hyeun ; Park, Kyung-Soo ; Park, Jin-Woo ; Park, Kyung-Seok ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 275~282
The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.
Characteristics of Phytophthora capsici Causing Pepper Phytophthora Blight Resistant to Metalaxyl
Lee, Soo-Min ; Shin, Jin-Ho ; Kim, Sun-Bo ; Kim, Heung-Tae ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 283~289
Isolation frequency of resistant isolates of Phytophthora capsici to metalaxyl was reported to be 38.9% through the resistance monitoring for metalaxyl in P. capsici causing pepper Phytophthora blight. Metalaxyl was very effective to mycelium growth, while not to zoosporangium germination and zoospore release.
values of metalaxyl in the inhibition of mycelium growth were 0.204, 0.151, 0.379, and
against each isolate sensitive to the fungicide as P. capsici 06-119, 06-143, P08-7, and P08-31, respectively, whilst those were 5.242, 5.724, 6.621, and
in P. capsici 06-125, 06-155, P08-50, and P08-60. For the field fitness, several factors, which were mycelium growth, zoosporangium germination, zoospore release, virulence to pepper plants, and the zoosporangium and the oospore production, were investigated with 4 sensitive isolates and 4 resistant isolates. Between 2 groups differentiated by the sensitivity of metalaxyl, there was no significance in mycelium growth, zoosporangium germination, zoospore release, and virulence to pepper plants. However, the zoosporangium and the oospore production in each resistant isolate, which were related to survival of P. capsici in fields, were superior to those of sensitive isolates. Based on results of this study, it was suggested that the increase of the percentage of resistant isolates to metalaxyl resulted from the high capacities of the zoosporangium and the oospore production.
Characterization of the Acetolactate synthase (ALS) gene and Molecular Assay of Mutations Associated with Sulfonylurea Herbicide Resistance of Monochoria vaginalis
Park, Tae-Seon ; Park, Hong-Kyu ; Ku, Bon-Il ; Kim, Young-Doo ; Ko, Jae-Kwon ; Lee, In-Yong ; Park, Jae-Eup ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 290~297
This research aims to contribute the characterization of acetolactate synthase (Ec 126.96.36.199; ALS) and the resistance mechanism by sequence analysis of ALS gene of the sulfonylurea-resistant and -susceptible Monochoria vaginalis. The ALS gene was obtained from susceptible (S) and resistant (R) M. vaginalis to sulfonylurea herbicides (SUs). The 815 bp the fragment and the genomic DNA sequence coding for acetolactate synthase (ALS) of S and R biotypes of M. vaginalis were cloned and sequenced. Nineteen clones were divided greatly into 4 groups as result of sequencing. The first group was not difference to S type, the second group was amino acid of P197S which found point mutations causing substitution of serine for proline at amino acid 197, the third group was observed greatly other part of 6 places than group 1, and the fourth group appeared the intergrade of group 1 and 3. Therefore, it could be assumed what ALS gene of various types can be one plant. The peptide of the 13 amino acid Domain A region for ALS genes from R biotype of M. vaginalis differed from that of the S biotype by one base substitution at proline codon of Domain A. It could also be confirmed that point mutation of serine for proline at amino acid 197.
Occurrence of Papaya ringspot virus Infecting Cucurbit Crops in Korea
Jin, Tae-Seong ; Kim, Sang-Mok ; Ko, Sug-Ju ; Lee, Su-Heon ; Choi, Hong-Soo ; Park, Jin-Woo ; Cha, Byeong-Jin ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 298~308
A flexuous rod-shaped virus was isolated from Cucurbita pepo leaves showing as green mosaic and puckering symptoms at Anseong, Korea. Based on the biological analysis, electron microscopy, and reverse transcription-polymerase chain reaction (RT-PCR), the virus isolate was identified as Papaya ringspot virus type watermelon (PRSV-W). From biological analysis, the host range of PRSV-W was limited to the families Cucurbitaceae and Chenopodiaceae. Most susceptible cucurbit species, such as Cucumis lanatus, Cucumis sativus, Cucurbita pepo, and Citrullus lanatus, showed symptoms of green mosaic, malformation, puckering, and narrow laminae by infection with PRSV-W. The local lesion were showed on the inoculated leaves of both Chenopodium amaranticolor and C. quinoa. Field survey of PRSV, Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV), three major viruses infecting cucurbit, was done during 2001 to 2003 on 173 commercial cucurbit cultivating fields distributed over the three regions of Gyeonggi, Gyeongbuk and Jeonnam Provinces where cucurbits are grown in different environmental conditions and cropping patterns. Typical viral symptoms were observed from 107 cultivating fields, and all three kinds of potyviruses were detected from 206 samples out of the 235 samples using RT-PCR. Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV) are the most widely distributed viruses in outdoor and retarding-culture fields, at an infection rating of 48 and 33 percents, respectively. PRSV was detected from 12 percent of 235 samples. The nucleotide and amino acid sequences of coat proteins (CP) of eight PRSV isolates, collected from several areas including Anseong, were determined and sequenced heterogeneity among the isolates was performed. The CP gene of PRSV showed 88.6~97.3 percent homology in nucleotide sequences and 95.1~99.3 percent homology in amino acid sequences with other PRSV isolates worldwide. The phylogenetic analysis indicated that the Korean PRSV isolates belong to the southern-east Asian cluster.
Incidence of Rice stripe virus during 2002 to 2004 in Korea and Chemical Control of Small Brown Plant Hopper
Park, Jin-Woo ; Jin, Tae-Seong ; Choi, Hong-Soo ; Lee, Su-Heon ; Shin, Dong-Bum ; Oh, In-Seok ; Lee, Sang-Guei ; Lee, Min-Ho ; Choi, Byeong-Ryeol ; Bae, Soon-Do ; Kim, Jin-Young ; Han, Kwang-Seop ; Noh, Tae-Hwan ; Ko, Sug-Ju ; Park, Jong-Dae ; Lee, Bong-Choon ; Kim, Tae-Sung ; Chung, Bu-Keun ; Hong, Sung-Jun ; Kim, Choong-Hoe ; Park, Hyung-Man ; Lee, Key-Woon ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 309~314
Incidence of rice stripe disease, caused by Rice stripe tenuivirus (RSV), was surveyed during 2002 to 2004. The incidence area and ratio of diseased fields were decreased gradually during those period. Rate of diseased plants were 45.8%, 45.0% and 43.7% in the susceptible cultivars Chucheong, Saechucheong and Ilpum, respectively. However, the rate was 4.4% in resistant cultivar Hwaseong. In addition, breakdown rate was also significantly high in the susceptible cultivars in Chucheong, Saechucheong and Ilpum showing 33.6, 33.2 and 31.9%, respectively. In Hwaseong, the breakdown rate was 0.8%. Collection efficiency was compared between two insect vector collection methods. Insect-sucking machine method was much more efficient than sweeping net method in collecting small brown plant hopper (SBPH). According to the survey of the population density of the insect vector during 2002 to 2004, the national average population density was gradually decreased year by year, 3.6, 2.3, and 1.3%, respectively. This result was significantly related with the decrease of the incidence of rice stripe disease. Control efficiency of rice stripe disease by treating several seedling box and water surface with insecticides against SBPH resulted that imidacloprid GR, Fipronil FG, Clothianidin+Probenazole GR and Thiamethoxam GR showed over 80% of control efficiency at 28 days after treatment at the early stage in nursery.
Insecticidal activity of Diamondback Moth, Plutella xylostella against Bacillus thuringiensis and Neem oil
Cho, Min-Su ; Choi, Su-Yeon ; Kim, Tae-Whan ; Park, Chan ; Kim, Dam-A ; Kim, Young-Rim ; Oh, Se-Mun ; Kim, Sung-Woo ; Youn, Young-Nam ; Yu, Yong-Man ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 315~324
For the environmental friendly management of diamondback moth, Plutella xylostella (L.), Bacillus thuringiensis subsp. kurstaki (
) and neem oil (0.5% azadirachtin) were used as green control agencies with mixed and alternative treatments on the chinese cabbage. When Bacillus thuringiensis subsp. kurstaki was applied to 1st and 2nd larva of P. xylostella with recommended concentration, their mortalities were reached to 100% by 2 days after treatment. In case of azadirachtin, its effect of mortality was continued for 7 days, and reached to 100% mortality.
values of Bacillus thuringiensis subsp. kurstaki and azadirachtin against 1st, 2nd, 3rd and 4th larva of P. xylostella were
, and 2.7, 3.9, 4.7 and 7.1 ppm, respectively. The number of laid eggs of P. xylostella was reduced to 57.5 at 25 ppm of azadirachtin compared with control treatment. The hatch ratio was not significantly different with Bacillus thuringiensis subsp. kurstaki treatment in comparison with control treatment. However, when azadirachtin was applied, their hatch ratio were reduced to 25.8 and 45.4% at 25 and 50 ppm, respectively. On the other hand, emergence rate of eggs was not different with Bacillus thuringiensis subsp. kurstaki treatment, but 45.4% was shown in azadirachtin treatment with 50 ppm in comparison with control. When the mixture with Bacillus thuringiensis subsp. kurstaki and azadirachtin was applied to adults of P. xylostella, their mortality was higher than Bacillus thuringiensis subsp. kurstaki treatment only. These results are supposed that the mixture of Bacillus thuringiensis subsp. kurstaki and azadirachtin might be used as green control agents for reducing the demage of diamondback moth in the Chinse cabbage.
Biological Control of Soft Rot on Chinese Cabbage Using Beneficial Bacterial Agents in Greenhouse and Field
Shrestha, Anupama ; Kim, Eun-Chang ; Lim, Chuen-Keun ; Cho, Sae-Youll ; Hur, Jang-Hyun ; Park, Duck-Hwan ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 325~331
Three beneficial bacterial agents, Lactobacillus strain KLF01, Lactococcus strain KLC02 and Paenibacillus strain KPB3 were showed clear zone against plated Pectobacterium carotovorum subsp. carotovorum (Pcc) soft rot pathogen. In greenhouse test, bio-control efficacy was more significantly effective in the treatments by KLC02 and KPB3 as 64%, 50%, 56% and 66%, 57%, 58% according to date of evaluation, respectively. In case of KLF01 control effect was relatively lower than treatments of KLC02 and KPB3 but its efficacy was still significantly observed when compared to control (only water treatment). Furthermore, KLF01, KLC02 and KPB3 showed 55%, 60% and 62% bio-control efficacy, respectively in field test from early March to late July of 2009. Thus, we suggest that these strains can be useful as bio-control agents against soft rot caused by Pcc.
Identification of Rhizo-bacterium Inhibiting Diaporthe citri Causing Citrus Melanose
Nnam, Myung-Hyeun ; Shin, Jin-Ho ; Choi, Jae-Pill ; Hong, Suck_Il ; Kim, Young-Gwon ; Kim, Heung-Tae ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 332~335
Rhizo-bacteria were isolated from organic-farming soils to select antagonistic agent for controlling citrus melanose disease. Among several antagonistic bacteria, KB-401 effectively inhibited mycelial growth of several plant fungal pathogens, including the pathogen of citrus melanose, Diaporthe citri. KB-401 also inhibited spore germination of the fungal pathogen. The tip of germ tube was swollen when conidia of D. citri were co-culture with KB-401 in PD broth amended 1% glucose. KB-401 was identified as Bacillus subtilis through the investigation for physiological characters and the analysis of nucleotide sequences of 16S rDNA.
Developmental Trend of Analytical Methods for Pesticide Residues
Ock, Hwan-Suck ;
The Korean Journal of Pesticide Science, volume 13, issue 4, 2009, Pages 336~348