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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 14, Issue 4 - Dec 2010
Volume 14, Issue 3 - Sep 2010
Volume 14, Issue 2 - Jun 2010
Volume 14, Issue 1 - Mar 2010
Selecting the target year
Monitoring and Risk Assessment of Pesticide Residues in Agricultural Products Collected from Wholesale and Traditional Markets in Cheongju
Noh, Hyun-Ho ; Kang, Kyung-Won ; Park, Young-Soon ; Park, Hyo-Kyung ; Lee, Kwang-Hun ; Lee, Jae-Yun ; Yeop, Kyung-Won ; Lee, Eun-Young ; Jin, Yong-Duk ; Kyung, Kee-Sung ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 1~9
In order to survey residual characteristics of pesticides in the agricultural products selling at markets and to assess their safety, a total of 120 agricultural products were collected from the wholesale and traditional markets in Cheongju and analyzed the pesticide residues in them by multiresidue analysis method using GLC, HPLC and GC-MSD. Three pesticides, procymidone, penconazole, and tetraconazole, were detected from 4 samples such as onion, leek, tomato, and green pepper. Fungicide penconazole was detected from the onion collected from wholesale market. Fungicide procymidone was detected from leek and tomato and fungicide tetraconazole was detected from green pepper. Pesticide residues were detected from 3.3% of the total samples. The estimated daily intakes (EDIs) of the pesticides detected were less than 0.1% of their acceptable daily intakes (ADIs), representing that residue levels of the pesticides detected were evaluated as safe.
Comparison of Toxic Response of Cladocerans to Organic Solvents to Establish the Standard Test Guidelines Using Korean Native Species
Kim, Byung-Seok ; Park, Yoen-Ki ; Yang, Yu-Jung ; Hong, Soon-Sung ; Park, Kyung-Hun ; Jeong, Mi-Hye ; Kim, Se-Ri ; Park, Kyeong-Hun ; Yeh, Wan-Hae ; Kim, Doo-Ho ; Yun, Jong-Chul ; Hong, Moo-Ki ; Kyung, Kee-Sung ; Ahn, Young-Joon ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 10~15
Most pesticides are poorly soluble in water and must be dissolved in a solvent carrier before being added to the test medium on aquatic toxicity test. The concentration of solvent is critical to the success of a test. This study were conducted to recommend possible organic solvents which have good solubility for pesticides and low toxicity to Korean native water flea to establish new standard toxicity test methods using Korean native water flea for ecological risk assessment of pesticide. Four Korean freshwater cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocephalus vetulus were exposed to 10 different organic solvents during 48 hours to evaluate their toxic response to solvents. Ethyl acetate was the most toxic to cladocerans tested. Although ethyl ether was the least toxic to cladocerans tested, it may not adequate as possible solvent in aquatic toxicity test due to high volatility and low water solubility. In conclusion, acetone, methanol, ethanol and acetonitrile which has low toxicity as well as good water solubility are recommended as optimal organic solvent to use in aquatic toxicity tests with Korean native cladocerans tested.
Analysis of Thiosultap in Hulled Rice by Derivatization
Choi, Jeong-Heui ; Do, Jung-Ah ; Yoon, Hae-Jung ; Park, Yong-Chun ; Kim, Jae-Hun ; Choi, Dong-Mi ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 16~20
Thiosultap, a nereistoxin analog insecticide, registered in China has been used to control selected beetles and Lepidopteran pests on rice, vegetables and fruit trees. Although domestic use of thiosultap is not permitted, it is needed to monitor this insecticide from imported crops because that has been used on crops in many foreign countries, especially China. Thiosultap in hulled rice was determined as nereistoxin derived in basic condition by GC-FPD. This method accomplished ion-associated liquid-liquid partitioning for cleanup, and limit of quantification and linearity performed by the established method were 0.05 mg
. The recoveries performed by control hulled rice fortified with thiosultap at 0.5 and 2.5 mg
Development of Analytical Method and Monitoring for Bifenazate in Commercial Agricultural Products
Park, Eun-Heui ; Go, Myoung-Jin ; Cho, Myong-Shik ; Kim, Young-Sun ; Lee, Jin-Ha ; Choi, Dong-Mi ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 21~29
The analytical method for bifenazate was developed using a HPLC (UVD). Also, analytical condition of LC/MS was set up for bifenazate. We validated the method for the precision and the reproducibility. The correlation coefficient of bifenazate ranged from 0.05 to 2.5 mg/kg was 1.0. Limit of quantitation (LOQ) was 0.01 mg/kg. To measure recoveries from agricultural products such as foxtail millet (cereal grains), kidney bean (beans), orange (fruits), perilla leaves (vegetables) and oak mushroom (mushrooms), bifenazate was spiked. Mean recoveries of bifenazate for each sample were 82.7~104.1% at the level of 0.1 mg/kg and 73.1~104.3% at the level of 0.5 mg/kg. The relative standard deviations (n=3) were 0.2~9.7%. Pesticide residues for bifenazate were investigated in 16 commodities (rice, foxtail millet, buckwheat, kidney bean, peanut, sesame, orange, grapefruit, kiwifruit, spinach, perilla leaves, leek, garlic stem, garlic, ginger and oak mushroom) collected from 22 provinces in 2009. Bifenazate was analyzed using analytical method by HPLC from 304 samples, and residue was not detected.
Characteristics on the Conversion of EBDCs Fungicides to ETU for Mancozeb and Metiram
Jang, Mi-Ra ; Hwang, Kwang-Eun ; Lee, Eun-Soon ; Kim, Eun-Hee ; Kim, Dong-Hyun ; KIm, Jung-Hun ; Park, Seog-Gee ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 30~36
This study was conducted to investigate the characteristics of conversion and residue for two EBDCs fungicides (mancozeb and metiram) and their metabolite, ethylenethiourea (ETU) under laboratory conditions. The sampling on the pesticides was done at 0, 1, 2, 5, 7, 9, 12, 15, 19, 22 and 26 days. The amounts of their residues and ETU were analyzed with an HPLC-DAD. The average recoveries were
for metiram, and
for ETU. The half-lives of mancozeb and metiram were 7.5 and 27.8 day, respectively. The conversion rates of mancozeb and metiram to ETU ranged from 4.7 to 67.2% and from 7.5 to 34.6%, respectively. These results may be different under the real environmental conditions. Therefore, following many studies are recommended to make the criteria for applying the use and safety for mancozeb and metiram.
Determination of Methoxyfenozide, Chromafenozide and Tebufenozide Residues in Agricultural Commodities Using HPLC-UVD/MS
Lee, Su-Jin ; Kim, Young-Hak ; Hwang, Young-Sun ; Kwon, Chan-Hyeok ; Do, Jeong-A ; Im, Moo-Hyeog ; Lee, Young-Deuk ; Choung, Myoung-Gun ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 37~48
The diacylhydrazine insecticides, methoxyfenozide, chromafenozide and tebufenozide are new-generation insecticides. These insecticides induce premature molting and cause the death of insects by mimicking their hormone. Also, these insecticides have already been widely used for vegetables planting in worldwide. Highperformance liquid chromatography (HPLC) is the most widely used procedure for determination of each compound residues in crops. However, simultaneous analysis method of these diacylhydrazine insecticides was not reported. The purpose of this study is to develop a simultaneous determination procedure of methoxyfenozide, chromafenozide and tebufenozide residue in crops using HPLC-UVD/MS method. These insecticide residues were extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, apple, pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover these insecticides from the aqueous phase. Florisil column chromatography was additionally employed for final cleanup of the extracts. The analytes were quantitated by HPLCUVD/MS, using a
column. The crops were fortified with each insecticide at two levels per crop. Mean recoveries ranged from 89.0 to 104.8% in five representative agricultural commodities. The coefficients of variation were less than 3.9%. Quantitative limits of methoxyfenozide, chromafenozide and tebufenozide were 0.04 mg/kg in crop samples. A HPLC-UVD/MS with selected-ion monitoring was also provided to confirm the suspected residues. The proposed simultaneous analysis method was reproducible and sensitive enough to determine the residues of methoxyfenozide, chromafenozide and tebufenozide in agricultural commodities.
Controlling Activity of Bacillus subtilis KB-401 against Cucumber Powdery Mildew Caused by Sphaerotheca fusca
Nam, Myung-Hyeun ; Choi, Jae-Pil ; Kim, Hyung-Jo ; Lee, Jae-Jun ; Lim, Keun-Hwan ; Kim, Young-Gwon ; Kim, Heung-Tae ; Jeun, Yong-Chull ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 49~53
Disease control efficacy was evaluated with use of Bacillus subtilis KB-401 against cucumber powdery mildew in a greenhouse and fields. B. subtilis KB-401 showing inhibitory effect on mycelial growth of various phytopathogenic fungi was formulated for the evaluation. The formulated biofungicide of B. subtilis KB-401 was less effective at 1,000 times dilution rate than that at 250 or 500 times dilution rate. The powdery mildew was successfully controlled by the biofungicide at the early stage of disease development. The field performance of the biofungicde was conducted in Asan and Cheonan city. Three or four consecutive applications of the biofungicide at 500 dilution rate with 10-day intervals resulted in considerable efficacy of disease control as high as 83.3%.
Effect of Neem and Mustard oils on Entomopathogenic Nematodes and Silkworm
Ha, Pan-Jung ; Kim, Tae-Su ; Lee, Shin-Hae ; Choo, Ho-Yul ; Choi, Sung-Hwan ; Kim, Young-Sub ; Lee, Dong-Woon ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 54~64
Effects of thirteen essential oils (anise oil, clove oil, marigold, mustard oil, neem oil, quassia, quilaja, rosemary oil, rotenone, tea tree extract, thyme oil, wintergreen oil, and yucca) and caffeine on typical industrial insect, silkworm (Bombyx mori) and two entomopathogenic nematodes, Steinernema carpocapsae GSN-1 strain (Sc) and Heterorhabditis sp. Gyeongsan strain (Hg) were investigated in the laboratory. When 1,000 ppm of each essential oils was treated, neem oil showed the highest insecticidal activity against silkworm. Mortality of silkworm fed on neem oil treated mulberry leaf was 55.3 and 100% 5 and 10 days after treatment, respectively. The silkworm fed on neem oil treated mulberry leaf did not make cocoon and pupa. Weight of cocoon and pupa was low in rotenone treatment showing 0.27 g and 1.01 g, respectively. Mustard oil had the highest nematicidal activity against entomopathogenic nematodes. 20 ppm of mustard oil resulted in 69.0% and 100% mortality of Sc and Hg 3 days after treatment, but 4% and 36% at 5 ppm in X-plate, respectively. Mortality of baited Galleria mellonella larva by Sc was not different from control at the concentration of 100 ppm of mustard oil while 30% lower in Hg in sand barrier. Mean numbers of established infective juveniles of Hg in Galleria larva were lower than Sc in sand barrier. Survival rate of Sc was similar to control at the concentration of <200 ppm of mustard oil in sand barrier.
Effect of Inoculum Concentration of Rhizoctonia solani and Pythium ultimum Causing Damping-off of Pepper and Cucumber on the Efficacy of the Mixture of Etridiazole and Thiophanate-methyl
Kim, Hyung-Jo ; Jang, Ho-Sun ; Lee, Soo-Min ; Kim, Joo-Hyung ; Shin, Jin-Ho ; Kim, Heung-Tae ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 65~71
This study was performed to investigate the inhibiting activity of etridiazole and thiophanate-mthyl on mycelial growth of Rhizoctonia solani and Pythium ultimum and the effect of inoculum density of each pathogen on the control efficacy of the mixture of etridiazole and thiophanate-methyl in a seedling assay test. In mycelial growth inhibition test,
values of etridiazole and thiophanate-methyl against R. solani were 15.87 and 9.34
, while those were 0.2 and more than
against P. ultimum, respectively. Controlling activity of the mixture of etridiazole and thiophanate-methyl against damping-off of pepper and cucumber, caused by R. solani and P. ultimum was tested in a greenhouse. With the inoculation of R. solani, disease incidences of seedling in pepper were 73% and 95% at 0.5% and 1.0% of inoculum concentration, while in cucumber those were 55% and 62% at 1.0% and 2.0%. When P. ultimum was inoculated into soil by 2.0% of inoculum concentration, those in pepper and cucumber were 66.7% and 96.8%, respectively. The efficacy of the mixture was somehow affected by the concentration of R. solani. While each control value of the mixture was 94.4% and 90.7% in pepper and cucumber at low inoculum concentration (0.05%), the efficacy of the fungicide decreased in pepper and cucumber by 70.7% and 72.9% at high concentration of R. solani (0.1% in pepper and 0.5% in cucumber). However, the control value of the mixture was 100% in pepper and cucumber, irrespective of the inoculum concentration of P. ultimum, however, the increase of inoculum concentration in soil did not result in the decrease of the fungicide efficacy.
Minimum Structural Requirements of R-phenoxy Substituents for Herbicidal Evaluation of O-(2-phenoxy)ethyl-N-aralkylcarbamate Analogues against Phytoene Desaturase
Choi, Won-Seok ; Lee, Jae-Whang ; Hwang, Seung-Woo ; Sung, Nack-Do ;
The Korean Journal of Pesticide Science, volume 14, issue 1, 2010, Pages 72~77
The minimum structural requirements of R-phenoxy substituents for herbicidal evaluation of O-(2-(R)-phenoxy)-ethyl-N-aralkylcarbamate (1-15) analogues against phytoene desaturase (PDS) based on the three dimensional quantitative structure-activity relationships (3D-QSARs: CoMFA and CoMSIA) were studied quantitatively. The correlativity and predictability (
) of the CoMFA 1 model were higher than those of the rest models. The PDS inhibitory activities from the optimized CoMFA 1 model were depend upon the steric field (44.0%), electrostatic field (36.3%), and hydrophobic field (19.6%) of O-(2-(R)-phenoxy)ethyl-Naralkylcarbamate analogues. From the CoMFA contour maps on the structure of the most active compound (5), if it has the steric favor at meta-, para-position on the phenoxy ring, the negative charge favor in meta-position and positive charge favor in the outside part of para-position, the inhibitory activity will be predicted to increase. Also, if ortho-, para-position, and outside of phenoxy ring are hydrophilic favor, and meta-position is hydrophobic favor, it is predicted that the inhibitory activity against PDS will be able to increase.