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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 15, Issue 4 - Dec 2011
Volume 15, Issue 3 - Sep 2011
Volume 15, Issue 2 - Jun 2011
Volume 15, Issue 1 - Mar 2011
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Studies on the Solar Activated Insecticidal Activities of Dihydroxyl Phosphorus(V) Triazatetrabenzocorrole Derivatives
Oh, Hyun-Chul ; Woo, Je-Wan ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 87~96
To study solar activated insecticide, three types of dihydroxyl phosphorus(V) triazatetrabenzocorrole derivatives (
) including H, 4'-methoxy phenoxy, 4'-tert butyl phenoxy substituents were synthesized. The results show that slightly red-shift with introducing substituents was observed and singlet oxygen was generated by the sunlight. Based on photochemical properties, solar activated insecticidal activity tests against Liriomyza trifolii (Burgess) were carried out. Overall insecticidal activities were 100~85.7% in the concentration of 500ppm, and especially in the case of compound
the insecticidal activities was 100%.
Residue Patterns of Azoxystrobin and Cyenopyrafen In Grape between Rainshield and Plastic House Conditions
Lee, Cho-Rong ; Hong, Ji-Hyung ; Lim, Jong-Sung ; Lee, Kyu-Seung ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 97~103
The residual patterns of azoxystrobin and cyenopyrafen were investigated to compare the differences between rainshield and plastic house conditions of the grapes. Initial residue concentrations of azoxystrobin were
under rainshield condition and
under plastic house condition. And cyenopyrafen was 0.55 and
respectively. Biological half-life of azoxystrobin was calculated as 13.28 days under rainshield and 15.58 days under plastic house condition. And cyneopyrafen was 7.99 and 12.65 days, respectively. In comparison with two different cultivation conditions, pesticides in/on the grape were decomposed more faster under plastic house than rain shield conditions.
Thirteen-Week Repeated Oral Toxicity Study of Leaf Folder (Cnaphalocrocis medinalis) Resistant Rice (Nakdongbyeb) in Sparague-Dawely Rats
Seo, Dong-Seok ; Kwon, Min ; Sung, Ha-Jung ; Park, Cheol-Beom ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 104~113
We investigated the systemic effects of leaf folder (Cnaphalocrocis medinalis) resistant rice (transgenic rice, Nakdongbyeb) in Sprague-Dawley rats for 13 weeks. Leaf folder resistant rice was added to the diet at percentage levels of 0, 5 and 20 percentage/feeder and was administered for 13 weeks. The results did not show any changes in food and water intake. There were also no biologically significant changes in both body and organ weights, hematological and blood biochemical parameters, autopsy and histopathology between the treatment and control groups. Based on these results, no observed adverse effect level (NOAEL) of transgenic rice was considered to be more than 10,000 mg/kg b.w. under the conditions of the present study.
The Survey on Pesticide Residues in Vegetables Collected In Seoul
Jang, Mi-Ra ; Moon, Hyun-Kyung ; Kim, Tae-Rang ; Yuk, Dong-Hyun ; Kim, Eun-Hee ; Hong, Chae-Kyu ; Choi, Chae-Man ; Hwang, In-Sook ; Kim, Jung-Hun ; Kim, Moo-Sang ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 114~124
The characteristics of pesticide residues by multiresidue analysis method using GC, HPLC and GC-MSD were examined for 18,069 numbers of 91 kinds of vegetables in Seoul from 2007 to 2009. Detection rates of pesticide residues were 11.2% in 2007, 8.5% in 2008 and 12.0% in 2009, respectively, and the excess rates of Maximum Residue Limits (MRLs) showed a declining tendency as 4.6% in 2007, 2.8% in 2008 and 2.1% in 2009, respectively. The pesticides exceeding MRLs were 43 ingredients in 2007, 30 ingredients in 2008 and 36 ingredients in 2009, respectively. The frequencies of pesticides exceeding MRLs were 71 of azoxystrobin in 2007, 29 of procymidone in 2008, 24 of endosulfan in 2009, respectively. The excess rate of mustard green for MRLs in comparison with sample numbers was the highest among vegetables analyzed more than 10 cases each year. As a result of comparing annually pesticide residues about pesticide ingredients showed high frequency rates for pesticide detection, there was a statistical significance for the detection quantities of diazinon, endosulfan, paclobutrazol and procymidone.
Determination of Ametryn Residue in Agricultural Commodities Using HPLC-UVD/MS
Lee, Su-Jin ; Kim, Young-Hak ; Song, Lee-Seul ; Choung, Myoung-Gun ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 125~133
Ametryn is used in USA, China, and Japan, but not introduced in Korea yet. So, MRL (Maximum Residue Level), and analytical method of ametryn were not establishment in Korea. Therefore, this experiment was conducted to establish a determination method for ametryn residue in crops using HPLC-UVD/MS. Ametryn residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover ametryn from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The ametryn was quantitated by HPLC with UVD, using a Tosoh ODS 120T (
mm) column. The crops were fortified with ametryn at 2 levels per crop. Mean recovery ratio were ranged from 83.7% for a 0.2 mg/kg in soybean to 91.1% for a 1.0 mg/kg in hulled rice. The coefficients of variation were ranged from 1.2% for a 1.0 mg/kg in hulled rice to 3.6% for a 1.0 mg/kg in soybean. Quantitative limit of amatryn was 0.02 mg/kg in representative 5 crop samples. A LC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of ametryne in agricultural commodities.
Pesticide Residue Monitoring and Environmental Exposure in Paddy Field Soils and Greenhouse Soils
Park, Byung-Jun ; Lee, Ji-Ho ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 134~139
To investigate an amount of pesticide residue in rice paddy field soils and greenhouse soil, this monitoring was carried out pesticide detection frequency and concentrations collected samples from 150 rice paddy field soils and 152 greenhouse soils of nationwide in the year of 2007, and 2008, respectively. The detection limit of pesticides of this experiment were ranged 0.001~0.005 ppm. In 2007, One hundred fifty samples were collected from rice paddy field soils in April and monitored for 120 wide-used pesticides. A total of 11 pesticides were detected four fungicides, four insecticides and three herbicides in paddy field soils. The highest concentration levels of pesticide detected were 0.84 ppm as herbicide oxadiazon, 0.81 ppm as fungicide isoprothiolane and 0.50 ppm as insecticide buprofezin. The detection frequencies range were 0~19.3%, and the frequency was 2.7% as isoprothiolane and 19.3% as oxadiazon in paddy field soils. In 2008, One hundred fifty two samples were collected from greenhouse soils in April and monitored for 120 wide-used pesticides. A total of 29 pesticides were detected six fungicides, sixteen insecticides and seven herbicides in greenhouse soils. high concentration levels of pesticide detected levels were 5.09 ppm as insecticide chlorfenapyr, 2.57 ppm as fungicide chlorothalonil and 0.72 ppm as herbicide oxadiazon. The detection frequencies range were 0~38.8%, and high frequencies were 38.8% as insecticide endosulfan, 13.2% as oxadiazone, 10.5% as fungicide hexaconazole and 7.2% as isoprothiolane in greenhouse soils, Total endosulfan and oxadiazon were showed high detection frequency of 38.8% and 13.2%, respectively.
Improvement of Analytical Method for Residue Pesticides in Herbal Medicines Using Macroporous Diatomaceous Earth Column
Hwang, Jeong-In ; Jeon, Young-Hwan ; Kim, Hyo-Young ; Kim, Ji-Hwan ; Ahn, Ji-Woon ; Seok, Da-Rong ; Lee, Yoon-Jeong ; Park, Ju-Young ; Kim, Do-Hoon ; Kim, Jang-Eok ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 140~148
The official analytical method of residue pesticides in herbal medicines by KFDA cannot be applied to all of the pesticides and herbal medicines because of various active materials in herbal medicines and various physicochemical properties of pesticides. Moreover, liquid-liquid partition uses harmful solvents such as methylene chloride and is consuming a lot of time and effort. In order to improve the problems, we have studied for the availability of the analytical method applying the macroporous diatomaceous earth (MDE) column instead of liquid-liquid partition to simultaneously analyze five pesticides in two dried herbal medicines. The results showed that the recovery rates of acetamiprid and azoxystrobin in Astragalus root by GC/ECD ranged from 89.6 to 94.1%, from 86.8 to 94.4%, respectively, and those of bifenthrin, chlorfenapyr, chlorpyrifos in Cnidii Rhizoma by GC/MS ranged from 83.6 to 88.4%, from 77.4 to 83.8%, from 82.6 to 84.3%, respectively. Also, the coefficients of variation (CV) for triplication ranged from 0.5 to 1.7%. The results satisfied the criteria of residue pesticide analysis, setting 70~120% for the recovery rate and below 10% for the coefficient of variation. The improved methods are safer to residue pesticide analysts, faster and less laborious than the KFDA official method.
Analytical Method of Bentazone Residue in Agricultural Commodities Using HPLC-UVD/MS
Kim, Young-Hak ; Lee, Su-Jin ; Song, Lee-Seul ; Hwang, Young-Sun ; Lee, Young-Deuk ; Choung, Myoung-Gun ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 149~159
Bentazone is benzothiadiazole group herbicide, and used to foliage treatment. This herbicide have already been widely used for cereals and vegetables planting in worldwide. This experiment was conducted to establish a determination method for bentazone residue in crops using HPLC-UVD/MS. Bentazone residue was extracted with acetone (adjusted pH 1 with phosphoric acid) from representative samples of five raw products which comprised hulled rice, soybean, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover bentazone from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The bentazone was quantitated by HPLC with UVD, using a YMC ODS AM 303 (
mm) column. The crops were fortified with bentazone at 3 levels per crop. Mean recovery ratio were ranged from 82.0% for a 0.2 mg/kg in apple to 97.9% for a 0.02 mg/kg in Chinese cabbage. The coefficients of variation were ranged from 0.5% for a 0.02 mg/kg in soybean to 9.7% for a 0.02 mg/kg in Chinese cabbage. Quantitative limit of bentazone was 0.02 mg/kg in representative five crop samples. A LC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of bentazone in agricultural commodities.
Monitoring of Residual Pesticides in Agricultural Land from the Southern Area of Seoul
Choi, Chae-Man ; Yook, Dong-Hyeon ; Hong, Chae-Kyu ; Kim, Tae-Rang ; Hwang, Young-Sook ; Hwang, In-Sook ; Kim, Jung-Hun ; Kim, Mu-Sang ; Chae, Young-Zoo ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 160~165
Monitoring of pesticide residues were conducted at three sites in the Southern Area of Seoul, Korea (Gang-nam, Gang-dong and Song-pa). We measured pesticide residues within soil samples using multi-pesticide residues analysis. Samples were collected at the three sites with 60 sampling spots. The amount of pesticide residues in the soil samples were as follows [(minimum-maximum), mg/kg] endosulfan 0.002-0.999, procymidone 0.002-1.200, diazinon 0.003-0.024, metalaxyl 0.012-0.075. In soil treated with 0.445 mg/kg of endosulfan, the pesticide absorbed by chamnamul, was 0.157 mg/kg (Absorption rate : 35.3%). However, in soil treated with 0.358 mg/kg of endosulfan, the pesticide absorbed by lettuce, was 0.004 mg/kg (Absorption rate : 1.1%).
Insecticidal Activity and Molecular Characteristics of Bacillus thuringiensis CAB530 Isolated from Anomala albopilosa (Rutelidae: Coleoptera)
Beom, Jong-Il ; Seo, Mi-Ja ; You, Joo ; Youn, Young-Nam ; Yu, Yong-Man ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 166~176
Bacillus thuringiensis CAB530 was isolated from dead Anomata albopilosa (Rutelidae: Coleoptera) and soil of green tea field, and confirmed its insecticidal activities. CAB530 isolate showed a high insecticidal activity against the beet armyworm among the many lepidopteran insects that are difficult to control.
value of CAB530 isolate against the second larva of Spodoptera exigua was
spore concentration (cfu/
). SDS-PAGE result of insecticidal toxin protein of CAB530 isolate showed a band at 130 kDa that is similar pattern with B. thuringiensis subsp. kurstaki that took insecticidal activity against S. exigua. Otherwise, the crystal protein of the CAB530 isolate was conformed at 65 kDa level after 30 minute of incubation in S. exigua midgut juice. Six crystal genes (cry1Aa, cry1Ab, cry1C, cry1D, cry1F and cry1I) were identified by PCR. It different from genes of B. thuringiensis subsp. kurstaki. Crystal shape and pattern of toxin protein was similar with B. thuringiensis subsp. kurstaki, however, insecticidal activity and PCR result of CAB530 isolate was similar with B. thuringiensis subsp. aizawai.
Insecticidal Activities of Polymers and Surfactants Against Sweet Potato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
Yoon, Chang-Mann ; Cho, Sun-Ran ; Moon, Sang-Rae ; Shin, Youn-Ho ; Kim, Gil-Hah ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 177~187
This study was performed to evaluate the polymers and surfactants as the potential control agents of sweet potato whitefly Bemisia tabaci, which is causing problems in ornamental garden and greenhouse. Polymers have an insecticidal activity to knock down and to be lethal to small winged insects by its viscosity. Among five polymers tested at 0.2% concentration, polinol P-24 showed the highest insecticidal activity as 59.4% against B. tabaci adult in cylindrical chamber, and followed by polinol P-20 (insecticidal activity, 57.1%). When treated at 0.1 % or 0.3% concentrations, Polinol P-24 also showed the highest insecticidal activity with 43.3% and 54.5%, respectively. Among eight surfactants tested, insecticidal activity was the highest in 0.0005% NP10 treatment (70.0%), and followed by 0.001% NP7 (67.4%). The synergistic effect between polinol P-24 and eight surfactants was evaluated. After bioassays, the 0.2% polinol P-24 plus 0.005% NP10 was selected as a candidate control agent for controlling of B. tabaci adults. Polinol P-24/NP10 was showed the highest control efficacy against B. tabaci adults applied three times at three day-intervals in square rearing cage. In the greenhouse, the mixture treatment showed good control value over 70% seven days after treatment.
Sensitivity of Botrytis cinerea Isolated from Infected Leaves of Ginseng to Tolyfluanid
Kim, Joo-Hyung ; Kim, Ga-Hye ; Kim, Heung-Tae ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 188~193
Resistance monitoring for tolyfluanid was conducted over 2 years from 2005 to 2006 with 218 isolates of Botrytis cinerea obtained from infected plants of ginseng. All isolates of B. cinerea were divided into 3 groups such as highly sensitive (HS) group, sensitive (S) group and less sensitive (LS) group to tolyfluanid. HS group was defined as one showing
value less than
value of S group ranged between
, while that of LS group was more than
Among B. cinerea isolates tested in this study, 174 isolates were included into S group, while 22 isolates were done into HS group. The other isolates belonged to LS group. Botrytis cinerea isolates showed the high correlation of
value of mycelial growth with that of spore germination to tolyfluanid. This results could be used for the determination of fungicide resistance and the establishment of strategy for fungicide resistance management.
Development of a Bioassay Method Using Aluminium Foil Sheet for Screening Ovicidal Activity Against Diamondback Moth Egg, Plutella xylostella L. and Selection of Plant Extracts with High Ovicidal Activity
Kwon, Min ; Kim, Ju-Il ; Lee, Seung-Hwan ; Lin, Mei-Ying ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 194~200
Diamondback moth (DBM, Plutella xylostella L.) is known as the most destructive pest of cruciferous crops worldwide. As most insecticides targeted to mainly larval stage, new insecticides which have hatching-inhibitory or ovicidal activity could be more efficient to control DBM. Therefore, we developed an easy and efficient method for screening ovicidal activity of DBM eggs using aluminum foil. The aluminum foil (
cm) coated with filtered juice of chinese cabbage leave (hereinafter called oviposition foil) exposed to 300 newly-emerged adults for 24 hours inside the rearing container. The oviposition foils were replaced every 4 days consecutively after mating, but it was better to discarded over then. Oviposition foil were divided into 6 to 12 pieces depending on egg mass volume. After dipping into test solutions for 10 seconds using faucet, oviposition foil pieces were placed into common petri dish, and then investigated hatchability. The effect of methanol solvent (50%) for 10 seconds dipping on the toxicity against DBM eggs was negligible. In addition, whether covering the petri dish or not should be dependent on nature of active compounds tested. With applying the new bioassay method, methanol extracts from 50 plants were tested the ovicidal activity to DBM eggs. Among them, four plant extracts; Angelica tenuissima root, Lycium chinense root, Cnidium officinale root and Polygala tenuifolia root, showed high ovicidal activity of over 90% control efficacy, against DBM eggs.
Introduction of Globally Harmonized System for Agrochemical Products
Jeong, Sang-Hee ; Park, Cheol-Beom ; Han, Bum-Seok ; Kang, Chang-Soo ; Jeong, Mi-Hye ; Sung, Ha-Jung ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 201~207
The use of chemical products to enhance and improve life is a widespread worldwide practice. In spite of the benefits of these products, there is the potential of chemicals for adverse effects to people or the environment. The globally harmonized system (GHS) of classifying and labeling chemicals that was recommended by the United Nations in 2003, has been introduced globally since 2008. Compare to the classification criteria of agricultural formulations today, classification criteria of GHS is different partly. One pictogram is removed and 3 pictograms are introduced newly. The classification criteria of GHS will be changed preferentially and implemented gradationally to hazard products.
Toxicity Assessment and Establishment Acceptable Daily Intake of Pyrimisulfan
Jeong, Mi-Hye ; Park, Kyung-Hun ; Park, Jae-Eup ; Kwack, Seung-Jon ; Kim, Young-Bum ; Han, Bum-Seok ; Son, Woo-Chen ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 208~217
Pyrimisulfan is a herbicide. In order to register this new pesticide, the series of toxicity data on animal testing were reviewed to evaluate its hazards to consumers and also to determine its acceptable daily intake. Pyrimisulfan was excreted mostly by feces. It has low acute oral toxicity while it has no dermal, ocular irritation and skin sensitization (As the result of subchronic and chronic toxicity and carcinogenicity showed changes of hematology and liver.). Two-generation reproduction toxicity, genotoxicity, carcinogenicity and prenatal development toxicity were not proven. Therefore, the ADI for Pyrimisulfan is 0.1 mg/kg/ bw/day, based on the NOAEL of 10 mg/kg/ bw/day of 90-days repeated dose oral toxicity study in dogs while applying an uncertainty factor of 100.
Toxicity Assessment and Establishment Acceptable Daily Intake of Lepimectin
Jeong, Mi-Hye ; Hong, Soon-Sung ; Park, Kyung-Hun ; Park, Jae-Eup ; Kwack, Seung-Jun ; Kim, Young-Bum ; Han, Bum-Seok ; Son, Woo-Chen ;
The Korean Journal of Pesticide Science, volume 15, issue 2, 2011, Pages 218~229
Lepimectin is a insecticide agent. In order to register this new pesticide, the series of toxicity data on animal testing were reviwed to evaluate its hazards to consumers and to determine its acceptable daily intake. Lepimectin was mostly excreted by feces. It has low acute oral toxicity while it has no dermal, ocular irritation and skin sensitization (As the result of subchronic, chronic toxicity and carcinogenicity showed changes of hematology and clinical biochemistry parameter of serum and blood.). Two-generation reproduction toxicity, genotoxicity, carcinogenicity and prenatal development toxicity were not proven. Therefore, the ADI for Lepimectin is 0.02 mg/kg/ bw/day, based on the NOAEL of 2.02 mg/kg/ bw/day of two-years carcinogenic toxicity study in rats and applying an uncertainty factor of 100.