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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
Editor in Chief :
Volume & Issues
Volume 16, Issue 4 - Dec 2012
Volume 16, Issue 3 - Sep 2012
Volume 16, Issue 2 - Jun 2012
Volume 16, Issue 1 - Mar 2012
Selecting the target year
A survey on pesticide residues of imported fruits circulated in Gyeonggido
Cho, Yun-Sik ; Kang, Jeong-Bok ; Kim, Yang-Hee ; Jeong, Jin-A ; Huh, Jeong-Weon ; Lee, So-Hyun ; Lim, Young-Sik ; Bae, Ho-Jeong ; Kang, Heung-Gyu ; Lee, Jeong-Hee ; Jung, Eun-Sook ; Lee, Byoung-Hoon ; Park, Yong-Bok ; Lee, Jong-Bok ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 195~201
DOI : 10.7585/kjps.2012.16.3.195
We tested for pesticide residues in 124 samples of 22 different items of imported fruits circulated in Gyeonggido. Total 218 pesticides were analyzed by multi-residue method using gas chromatography/nitrogen phosphorus detector-electron capture detector (GC/NPD-ECD), time of flight/mass spectrometer (TOF/MS), ultra performance liquid chromatography/photo diode array (UPLC/PDA), high performance liquid chromatography/fluorescence detector (HPLC/FLD) and mass spectrometer (LC/MS/MS). The pesticides were detected in 18 fruits samples, ranging 0.003~0.3 mg/kg and no samples had violative residue. The separation test to 14 sample pesticides detected was conducted to monitor the current status of pesticide residues according to the partial characteristic. The pesticides were detected in 14 peels ranging 0.03~1.5 mg/kg and 2 fleshes in less than detection limits. These results indicate that imported fruits are safe when the human takes normally but even the small amount of pesticides is harmful when the human takes it in a prolonged period. Therefore, the pesticide residual amounts of imported friuts should be constantly monitored for food safety.
Determination of cyromazine residues in agricultural commodities using HPLC-UVD/MS
Song, Lee-Seul ; Kim, Young-Hak ; Lee, Su-Jin ; Hwang, Young-Sun ; Kwon, Chan-Hyeok ; Do, Jung-Ah ; Oh, Jae-Ho ; Im, Moo-Hyeog ; Chang, Woo-Suk ; Lee, Young-Deuk ; Choung, Myoung-Gun ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 202~208
DOI : 10.7585/kjps.2012.16.3.202
A high-performance liquid chromatographic (HPLC) method was developed to determine residues of cyromazine, a triazine insecticide, in agricultural commodities. Cyromazine was extracted with 90% aqueous methanol from representative crops which comprised brown rice, oyster mushroom, oriental melon, watermelon, and Chinese cabbage. Following to evaporation of methanol in the extract, the aqueous concentrate was acidified to form the protonated cyromazine. Dichloromethane partition was then applied to remove nonpolar co-extractives in the aqueous phase. Strong cation-exchange chromatography using Dowex 50W-X4 resin was employed for final purification of the extract. Cyromazine was successfully separated on a Zorbax SB-Aq
column showing high retention for polar compounds. Cyromazine was sensitively quantitated by ultraviolet absorption at 214 nm. Limit of quantitation (LOQ) of the method was 0.04 mg/kg irrespective of sample types. Each crops were fortified at 3 different concentrations of cyromazine for recovery test. Mean recoveries from samples fortified at LOQ~2.0 mg/kg in triplicate ranged 80.2~103.3% in five agricultural commodities. Relative standard deviations in recoveries were all less than 6%. A selected-ion monitoring LC/MS method with electrospray ionization in positive-ion mode was also provided to confirm the suspected residue. The proposed method was reproducible and sensitive enough to routinely determine and inspect the residue of cyromazine in agricultural commodities.
Isolation and identification of antifungal compounds from Spatholobus suberectus Dunn
Hwang, Joo-Tae ; Park, Young-Sik ; Kim, Young-Shin ; Kim, Jin-Cheol ; Lim, Chi-Hwan ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 209~216
DOI : 10.7585/kjps.2012.16.3.209
In the continued research on natural fungicides for control of plant diseases by using plant-derived products, we found that Spatholobus suberectus Dunn had a strong fungicidal activity against several plant pathogens. S. suberectus (1 kg) was extracted with 80% aqueous MeOH and then the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH and
successively. The four layers were tested their disease contron efficacies against 6 plant diseases such as rice blast (RCB), rice sheath blight (RSB), tomato grey mold (TGM), tomato late blight (TLB), wheat leaf rust (WLR), and barley powdery mildew (BPM). The EtOAc fraction was highly active showing over 80% control against RCB, TGM, TLB, and BPM. By using silica gel chromatography, preparative TLC and HPLC, six compounds that were expected to have antifungal activity were separated. Their chemical structures were identified as ethanone, hydroxytyrosol, epicatechin, procyanidin B2, dimethoxy daizein and formononetin by ESI-MS,
-NMR, and 2D-NMR spectroscopic analyses. The chemicals except epicatechin were first reported in S. suberectus. Study on in vitro and in vivo antifungal activities of the isolated compounds is in progress.
Residual characteristics of tolclofos-methyl treated by seed dressing in ginseng
Noh, Hyun-Ho ; Lee, Jae-Yun ; Park, So-Hyun ; Lee, Kwang-Hun ; Park, Hyo-Kyoung ; Oh, Jae-Ho ; Im, Moo-Hyeog ; Kwon, Chan-Hyeok ; Lee, Joong-Keun ; Woo, Hee-Dong ; Kwon, Ki-Sung ; Kyung, Kee-Sung ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 217~221
DOI : 10.7585/kjps.2012.16.3.217
This study was carried out to evaluate residual characteristics of tolclofos-methyl in ginseng and elucidate the reason for its high detection rate from fresh ginseng selling at markets. Seeds of ginseng were sowed after seed dressing with tolclofos-methyl and after a year of growth, the young seedlings were transplanted to field. They were then harvested annually until three-years of growth and the pesticide residue was analyzed in them. LOD and LOQ of the pesticide were 0.001 and 0.003 mg/kg, respectively. Recovery test was carried out to validate the analytical method for tolclofos-methyl in ginseng. The ginseng seedlings were fortified with the test pesticide at the level of LOQ, ten times of LOQ and maximum residue concentration of tolclofos-methyl. Its recovery ranged from 77.37 to 100.16%. Residual concentration of tolclofos-methyl in ginseng seedlings just before transplanting and two-year-old ginseng were from 7.58 to 8.05 and from 6.46 to 6.79 mg/kg, respectively. In case of three-year-old ginseng, it was found to be from 4.18 to 4.35 mg/kg. As a result of annual pesticide residue analysis, concentration of the pesticide was found to decrease time-coursely in ginseng. This may be due to decomposition and increasing of fresh weight of the ginseng during the cultivation periods of three years.
Processing factors of azoxystrobin in processed ginseng products
Lee, Jae-Yun ; Noh, Hyun-Ho ; Lee, Kwang-Hun ; Park, Hyo-Kyoung ; Oh, Jae-Ho ; Im, Moo-Hyeog ; Kwon, Chan-Hyeok ; Lee, Joong-Keun ; Woo, Hee-Dong ; Kwon, Ki-Sung ; Kyung, Kee-Sung ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 222~229
DOI : 10.7585/kjps.2012.16.3.222
This study was carried out to evaluate the residual characteristics of azoxystrobin in fresh ginseng and calculate its processing factors in processed products, such as dried ginseng, red ginseng and their extracts. Azoxystrobin was sprayed annually onto four-year-old ginseng according to its pre-harvest interval (PHI) for two years. Harvested ginsengs were processed according to the commercially well-qualified conventional methods provided by the Korea Ginseng Corporation. Limits of detection (LODs) of azoxystrobin in fresh ginseng and its processed products were 0.001 and 0.002 mg/kg, respectively. Also limits of quantitation (LOQs) in fresh ginseng and its processed products were 0.003 and 0.007 mg/kg, respectively. Recoveries of the analytical methods in fresh ginseng and its processed products ranged from 69.3 to 114.8%. Highest residue amounts in fresh ginseng and its processed products were 0.025 and 0.118 mg/kg, respectively. Processing factors of the processed products ranged from 1.85 to 3.17 in four-year-old ginseng and from 2.48 to 5.84 five-year-old ginseng.
Effect of field location and spray device on pesticide residue in chilli peppers
Son, Kyeong-Ae ; Kang, Tae-Kyeong ; Park, Byeong-Jun ; Kim, Taek-Kyum ; Gil, Geun-Hwan ; Kim, Chan-Sub ; Kim, Jin-Bae ; Im, Geon-Jae ; Lee, Key-Woon ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 230~235
DOI : 10.7585/kjps.2012.16.3.230
This study was carried out to clarify effects of field location and sprayer on the level of pesticide residue in chilli peppers. As confirmed by statistical analysis, the residue levels in green pepper among three greenhouses did not show significant difference at the first day after spraying with the same engine sprayer and nozzle. But the residue levels in green peppers collected from the exposed outside of crop were 2 times higher than those from the hidden inside. The sampling site was one of variation elements of pesticide residue. The residue levels after application by knapsack engine powered sprayer were 1.7 times higher than those by manual compressed sprayer. As the spraying pressure of the engine power sprayer is 2 times higher than the commonly used pressure of the manual compressed sprayer, the pressure of the sprayer and nozzles were considered to affect on the residue levels in peppers.
The residue characteristics of chlorpyrifos in chilli and sweet peppers
Son, Kyeong-Ae ; Kwon, Hye-Young ; Kim, Jin-Bae ; Jin, Yong-Duk ; Kim, Taek-Kyum ; Kim, Chan-Sub ; Gil, Geun-Hwan ; Im, Geon-Jae ; Lee, Key-Woon ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 236~241
DOI : 10.7585/kjps.2012.16.3.236
The characteristics of chlorpyrifos was studied to clarify the effect of the cultivation season, spray intervals, spray volume and concentration on residues in chilli and sweet peppers cultivated under greenhouse condition. Residue levels of chlorpyrifos detected in chilli pepper fruit cultivated were 1.5 to 2.7 times higher in winter(February-March) than those in summer(June-July). During winter season residue levels of chlorpyrifos in chilli peppers were 6.9~3.2 times higher than those in sweet peppers for 10 days after 3 times application with 7 days interval. Residue levels in chilli pepper were from 2.070 mg/kg at 1 day to 0.929 mg/kg at 10 day, while those in sweet pepper were from 0.302 mg/kg to 0.291 mg/kg. Residue levels in sweet pepper to which are 3 times applied with 7 days interval were from 0.302 mg/kg at 1 day to 0.291 mg/kg at 7 day, and the residue levels in sweet pepper which are 3 times applied with 3 days interval were from 0.498 mg/kg at 1 day to 0.470 mg/kg at 7 day. Residue levels of chlorpyrifos in the sweet peppers applied by double concentration were 2.5 times higher than those sprayed by normal standard amounts diluted in double volume.
Modified QuEChERS Multi-Residue Analysis Method for 61 pesticides in Fruits using with HPLC and GC-ECD/NPD
Lee, Ju-Young ; Hong, Su-Myeong ; Kim, Taek-Kyum ; Min, Zaw Win ; Kim, Yang-Hyeon ; Song, Kyung-Ae ; Kwon, Hye-Yong ; Lee, Hee-Dong ; Im, Geon-Jae ; Kim, Doo-Ho ; Kim, Jang-Eok ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 242~256
DOI : 10.7585/kjps.2012.16.3.242
QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method has been a lot of research for pesticide analysis, because it is very simple and fast. However, this method requires high sensitivity instrument such as LC-MS/MS because of the use of small sample volume and many impurities compared to the conventional method. So, QuEChERS method needs to be modified for using with HPLC and GC-ECD/NPD. The aim of this work was to study the application of the QuEChERS method as well as its modification for the extraction and preconcentration of 5 groups of 61 pesticides from 4 fruits prior to their determination by HPLC-PDA, GC-ECD/NPD, and LC-MS/MS. The method was validated using spiking levels at 0.1 mg/kg (or 0.01 mg/kg) in apple, grapes, pear and persimmon. The average recovery by QuEChERS AOAC Official 2007. 01 version using the LC-MS/MS varied from 71.1127.4% for 61 pesticides. The average recovery rates using modified QuEChERS varied from 70.9~126% for 61 pesticides by HPLC-PDA and GC-ECD/NPD. The results satisfied the criteria of multiple pesticide residue analysis, setting 70~130% for recovery rates and below 30% for CV.
Investigation of residual tricyclazole and its risk assessment in Korean sundried salts
Kim, Jin-Hyo ; Choi, Geun-Hyung ; Lee, Ji-Ho ; Kwon, Oh-Kyung ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 257~260
DOI : 10.7585/kjps.2012.16.3.257
Sundried salt was recently registered in food category in Korea in 2008, and food hygiene regulation only applied to several heavy metals and a few inorganic ions. In this report, we investigated the residual amounts of tricyclazole, a highly concerned contaminant from agricultural activity, and estimated their daily intake and hazard index. All 60 sundried salts were collected by region, and qualitatively and quantitatively were analyzed with GC-NPD. In this investigation, only three samples were confirmed the residual tricyclazole in sundried salts. Their maximum residue was
, and their estimated average daily intake was
. From these results, HI of tricyclazole for sundried salts was
for Koreans, and the values were not considered as serious risk issues currently.
The application of alternative methods for skin irritation evaluation on pesticides
Jeong, Mi-Hye ; Kim, Mi-Kyoung ; Park, Soo-Jin ; You, Are-Sun ; Hong, Soon-Sung ; Park, Kyung-Hun ; Park, Jae-Eup ;
The Korean Journal of Pesticide Science, volume 16, issue 3, 2012, Pages 261~266
DOI : 10.7585/kjps.2012.16.3.261
It is common to use many experiment animals to evaluate the toxicity of chemicals including pesticides. For protecting animal, the concepts of 3R (Reduction, Replacement, Refinement) were introduced and in vitro alternatives methods actively have been developed all over the world. Many experimental animals for toxicological tests have been used, so that it is important to establish the alternative methods. In this study, the alternative method using reconstituted human skin model (Keraskin
) was conducted for classification of skin irritation on pesticides. Sixteen formulations selected on the basis of the degree of irritation were treated by Keraskin
test. The percent of cell viability was measured into the culture medium collected after treatment of the pesticides for 24-72 hrs. The skin irritations of formulations were evaluated by the cell viability. In this study, The 4 formulations with mild irritation in rabbits were evaluated as nonirritant, the 6 formulations with moderate and severe irritation were evaluated as irritant in human skin model test. We suggest that the alternative test using Keraskin
model could be used as toxicity evaluation for primary irritation index (P.I.I.) score of greater than or equal to 2.1 of pesticides. The further studies should be required to apply for hazardous assessment of pesticides on alternative skin irritation methods because of the interindividual variability of the sensitivity of skin irritation on pesticides.