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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
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Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 18, Issue 4 - Dec 2014
Volume 18, Issue 3 - Sep 2014
Volume 18, Issue 2 - Jun 2014
Volume 18, Issue 1 - Mar 2014
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Determination of Pre-Harvest Residue Limits of Pesticides Metalaxyl-M and Flusilazole in Oriental Melon
Kim, Da Som ; Kim, Kyung Jin ; Kim, Hae Na ; Kim, Ji Yoon ; Hur, Jang Hyun ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 1~7
DOI : 10.7585/kjps.2014.18.1.1
The present study was performed to investigate the pre-harvest residue limit (PHRL) of pesticides namely, metalaxyl-M and flusilazole in oriental melon, and to identify the biological half-life and characteristics of their residues. In this study, pesticides were sprayed once as single spray and double spray on oriental melon. The oriental melon samples were collected at 0, 1, 2, 3, 5, 7, 9 and 11 days before harvest and samples were extracted with QuEChERS method. The residues of both the pesticides were quantified using GC/NPD and LC/MS/MS. The limit of detection was found to be 0.02 mg/kg and 0.01 mg/kg and their recoveries were greater than 95% (95.7% ~ 103.2% for metalaxyl-M and 100.2% ~ 106.8% for flusilazole) for both pesticides. The biological half-lives of both metalaxyl-M and flusilazole were 12 days at single and double spray, respectively. The PHRL of metalaxyl-M and flusilazole was found 1.0 mg/kg and 0.3 mg/kg, respectively for 10 days before harvest. The results of the present study shows the residual level of both the pesticides metalaxyl-M and flusilazole in oriental melon were less than their maximum residual limits.
Application of Neutral Red Uptake Assay Using EPC Cells as an Alternative to the Fish Acute Toxicity Test for Pesticide
Seo, Ji-Hyun ; Park, June-Woo ; Lee, Sung-Kyu ; Kim, Woo-Keun ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 8~13
DOI : 10.7585/kjps.2014.18.1.8
This study evaluated in vitro cytotoxicity of 5 pesticides, including 2 herbicides, 2 germicides, and an insecticide, as an alternative to the fish acute toxicity test. The in vitro cytotoxicity was tested using a neutral red uptake (NRU) assay with epithelioma papulosum cyprini (EPC) cells that originated from the epidermal tissue of Cyprinus carpio (common carp). An in vivo fish acute toxicity test was conducted according to OECD Test Guideline No. 203 using Aphyocypris chinensis (Chinese bleak), Oryzias latipes (Japanese medaka), and C. carpio. The results showed that the sensitivity of the cell viability assay for the pesticides was similar to the fish acute test in ranking order despite having approximately 10 times less absolute sensitivity. The
correlation values were calculated as 0.38 (p = 0.26), 0.76 (p = 0.05) and 0.90 (p = 0.01) for A. chinensis, O. latipes, and C. carpio, respectively. These results suggested that the potential of EPC cell viability assay as an alternative to the fish acute toxicity test due to their good correlation and NRU assay is expected to serve as a useful tool for predicting acute fish lethality for pesticides if further studies with a large set of pesticides are conducted.
Study on Development Effect on Zebrafish Embryo by Alacholr, Butachlor and Fipronil
Park, Soo Jin ; Jeong, Mihye ; Paik, Min-Kyoung ; Lee, Je-Bong ; You, Are-Sun ; Hong, SoonSung ; Ihm, Yang Bin ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 14~20
DOI : 10.7585/kjps.2014.18.1.14
This study is aimed to search the possibility of developmental toxicity test using the zebrafish from the pesticide. We selected herbicides alachlor and butachlor, reported for fish toxicity, and insecticide fipronil reported for the high fish toxicity and the honey bee risk among the pesticides with high usability for the examples of the pesticides in this experiment. In this study, we showed those effects on the zebrafish embryo development by exposing different kinds of pesticide with different concentration and exposed time periods. As a result, the rates of hatching and abnormality of the zebrafish embryo after treatments of alachlor were increased in 24-48 hpf group, and the juvenile fishes in every group exposed to
or more of alachlor displayed sever morphological changes such as bent tails, edema and activity failures. In case of the butachlor, the rates of hatching and the abnormality in 24-48 hpf group were higher than the other groups exposed in different time periods. The fatality before hatching was high in
or more of butachlor treatment, and entire zebrafish embryos in 48 hpf group died before hatching. All the living juvenile fishes showed morphological changes as like as the treatment of alachlor. The rate of hatching and the survival of the zebrafish embryo by the fipronil were higher than other pesticides. However, morphological changes such as bent tails were observed from the most of living juvenile fishes. Therefore, the effects of three different pesticides with different concentrations and exposing time periods on the development of zebrafish embryos showed that all the pesticides effects were proportional to the concentration, and exposing time periods may cause the morphological abnormality.
Toxicity Evaluation of Burkholderia pyrrocinia CAB08106-4 in Cyprinus carpio and Daphnia magna
Cho, Jae-Gu ; Kim, Mee-Seon ; Choi, Hyun-Jung ; Kwon, Min ; Kang, Tae-Gu ; Chung, Chang-Kook ; Kim, Kyun ; Oh, Seung-Min ; Park, Cheol-Beom ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 21~25
DOI : 10.7585/kjps.2014.18.1.21
Bukholderia pyrrocinia CAB08106-4 has an anti-fungal effect on Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp. It is environmentally friendly microbial product that prevents and controls a variety of phytopathogens including Garlic White Rot caused by Sclereotium cepivorum and Sclereotium sp. The aim of this study was to assess the environmental toxicity using Cyprinus carpio and Daphnia magna. Bukholderia pyrrocinia CAB08106-4 (
) was adminatrated to Cyprinus carpio and Daphnia magna according to the toxicity test guideline for peciticide.
of Bukholderia pyrrocinia CAB08106-4 is over
in Cyprinus carpio and Daphnia magna and no adverse effect was observed. Based on these results, we concluded that Bukholderia pyrrocinia CAB08106-4 has no toxiciy for Cyprinus carpio and Daphnia magna.
Control Efficacy of Environment-friendly Agricultural Materials against Alfalfa Weevil, Hypera postica Gyllenhal at Chinese Milk Vetch Field
Bae, Soondo ; Mainali, Bishwo Prasad ; Choi, Byeongryel ; Yoon, Youngnam ; Kim, Hyunju ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 26~32
DOI : 10.7585/kjps.2014.18.1.26
This study was conducted to evaluate pest control efficacy of environment-friendly agricultural materials (EFAMs) according to their spray time and frequency against alfalfa weevil, Hypera postica Gyllenhal, at Chinese milk vetch field in Miryang, Korea. Larva, pupa and adult of H. postica occurred more in year 2010 than those in 2011, and the larval population peaked 5 days faster in year 2010 than on
May in 2011. Control efficacies of EFAMS sprayed on various time and in several frequencies to H. postica during April in 2010 were 73.6%, 71.9% and 66.2% at single spray in early, middle and late April, respectively and ranged from 77.1% to 78.9% when sprayed twice. With three times spray the control efficacy averaged 87.2%. Control efficacies of EFAMs with a single application in early, middle and late April in 2011 were 57.9%, 66.8% and 65.2%, respectively and ranged from 73.7% to 76.8% when sprayed twice. Control efficacy averaged with 82.7% when EFAMs were sprayed for three times. Accordingly, control efficacy of EFAMs against was increased with increasing spray frequency. Among the tested, Ungsamee(R), Wangjoongwangeco(R) and Muchungjidae(R) with a single application showed relatively higher control efficacy with a 4 to 16% lower control rate than a chemical insecticide, etofenprox 20EC. Therefore, Ungsamee(R), Wangjoongwangeco(R) and Muchungjidae(R) can be effectively used for management of H. postica when sprayed at early April for high occurring population and middle April for low occurring population with a single spray.
Effect of Cyantraniliprole against of Bemisia tabaci and Prevention of Tomato Yellow Leaf Curl Virus (TYLCV)
Lee, Mun-Haeng ; Lee, Hee-Kyoung ; Lee, Hwan-Gu ; Lee, Sun-Gye ; Kim, Jeom-Soon ; Kim, Sung-Eun ; Kim, Young-Shik ; Suh, Jeung-Keun ; Youn, Young-Nam ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 33~40
DOI : 10.7585/kjps.2014.18.1.33
To control Bemisia tabaci on tomato, we applied five different combinations of chemical treatments as below: 1) treatment of combinations of cyantraniliprole on the root area and leaf with the existing registered chemicals three times; 2) treatment of combinations of cyantraniliprole on the root area and dinotefuran + emamectin benzoate on the leaf with the existing registered chemicals three times; 3) treatment of combinations of dinotefuran on the root area and cyantraniliprole on the leaf with the existing registered chemicals three times; 4) treatment of combinations of dinotefuran on the root area and dinotefuran + emamectin benzoate on the leaf with the existing registered chemicals three times; 5) untreated control plot (Table 1). Twenty days after treatment (
Aug.), the number of population of B. tabaci was zero on the 1, 2, 3, 4 treatments of combinations, and only 2 individuals were found on the 5 treatment of combination per each 20 plant. On
Sep., in the last observation, the average number of population of B. tabaci was 10.3, 10.3, 10.6 on the 1, 2, 3 treatments of combinations on the 20 plants per each combination, however, the average number of 23.3 and 37.6 were examined on the 4 and 5 treatments of combinations, respectively. TYLCV was not occurring on the 1 and 2 treatments of combinations, and presented only 3% and 17% on the 3 and 4 treatments of combinations, respectively, which indicates that the treatments (1-4) should be effective on TYLCV control as considering that 33% of TYLCV occurred on the untreated control plot. However, after the third flowering period, there is no difference among the five combinations. The amount of products was 9,148g and 9,698g on the 1 and 2 treatments of combinations, respectively, which was the most among the 5 combinations. The number of fallen fruits and the average weight of fruits showed the similar tendency.
Biological Monitoring of the Exposure Level of Organophosphorus and Pyrethroid Pesticides in Floriculture Workers and Florists
Song, Jae Seok ; Kwon, Ki Doo ; Choi, Hong Soon ; Yu, Ho Young ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 41~47
DOI : 10.7585/kjps.2014.18.1.41
This study was performed to evaluate the exposure level of organophosphorus and pyrethroid pesticide to floriculture workers and florists. The urinary dialkylphosphates, metabolites of organophosphorus insecticides, including dimethylphosphate (DMP), diethylphosphate (DEP), dimethylthiophosphate (DMTP), diethylthiophosphate(DETP) and pyrethroids of metabolites, cis/trans DCCA, DBCA, and 3-PBA were analysed to evaluate the exposure of organophosphorus and pyrethroid pesticide to floriculture workers and florists. The concentration of DMP is highest in floriculture workers. but the concentration of DETP is highest in retail florist. The concentration of 3-PBA is highest in floriculture workers. The amount of organophosphorus and pyrethroid pesticide expusure is highest in flower workers, wholesale florist and retail florists are followed. The management for reducing pesticide exposure to floriculture workers and wholesale florist is required.
Revision in the Codex Classification of Foods and Animal Feeds (2013)
Lee, Mi-Gyung ;
The Korean Journal of Pesticide Science, volume 18, issue 1, 2014, Pages 48~51
DOI : 10.7585/kjps.2014.18.1.48
Since the year of 2006 when the extended revision of the Codex Classification of Foods and Animal Feeds was undertaken, considerable progresses have been made in revising the Classification. This paper aimed to summarize the present status on revision of the Codex Classification of Foods and Animal Feeds, focusing remarkable achievements such as 1) the draft revision of the Codex Classification for the fruit commodity group and 2) the draft Principles and Guidance on the Selection of Representative Commodities for the Extrapolation of Maximum Residue Limits for Pesticides to Commodity Groups, adopted by the Codex Alimentarius Commission in 2012. Additionally, it included information on lists of crop group or subgroup which are holding at Step 7 and were adopted at Step 5, and further have not been yet discussed by the Codex Committee on Pesticide Residues. These information will be very helpful for a pesticide regulatory regime.