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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 18, Issue 4 - Dec 2014
Volume 18, Issue 3 - Sep 2014
Volume 18, Issue 2 - Jun 2014
Volume 18, Issue 1 - Mar 2014
Selecting the target year
Distribution Characteristics of Pesticide Residues in the Portions of Lettuce Leaves
Kwon, Sun-Mok ; Choi, Ok-Kyung ; Kim, Ki-Cheol ; Kim, Jung-Beom ; Kang, Heung-Gyu ; Cho, Yun-Sik ; Ha, Jin-Ok ; Jang, Jin-Ho ; Lee, Byoung-Hun ; Lee, Sung-Nam ; Lee, Sun-Young ; Kang, Suk-Ho ; Lee, Jong-Bok ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 53~60
DOI : 10.7585/kjps.2014.18.2.53
This study was conducted to investigate the changes of the residual amount in the leafstalk (end) and the fore-end (upper) portion of lettuce leaves during cultivation period to 14 days, 12 times in total (0, 1, 2, 3, 4, 5, 7, 8, 10, 12, 13 and 14 days) after spraying with boscalid and lufenuron. In the case of boscalid, the initial concentrations at 3 hours (0 day) of the leafstalk and the fore-end portion of lettuce leaves were 18.26 mg/kg and 84.97 mg/kg, respectively and the residual amounts were rapidly decreased to 0.31 mg/kg and 0.37 mg/kg at 14 days after chemical application. In the case of lufenuron, the initial concentrations at 3 hours (0 day) of the leafstalk and the fore-end portion of lettuce leaves were 0.91 mg/kg and 5.21 mg/kg, respectively and the residual amounts were rapidly decreased to 0.06 mg/kg and 0.09 mg/kg at 13 days after chemical application. The variations of the residual concentrations analyzing 12 times after spraying showed that the residual amounts of the leafstalk portion of lettuce leaves were less than its fore-end portion in boscalid and lufenuron. In additon, 9 kinds of pesticide including boscalid in 16 lettuce leaf (found to contain pesticide in 2013) showed that the residual amounts of the leafstalk portion of lettuce leaves were less than its fore-end portion as well.
Residual Patterns of Acaricides, Etoxazole and Flufenoxuron in Apples
Hwang, Jeong-In ; Kim, Jang-Eok ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 61~68
DOI : 10.7585/kjps.2014.18.2.61
Dissipation constants and half-lives of acaricides etoxazole and flufenoxuron in apples were calculated to establish their pre-harvest residue limits (PHRLs). The acaricides were sprayed on apples with single and triple doses based on safe use guidelines, and their residual patterns in the apple were interpreted using first order kinetics equation. The residual amounts of acaricides during the experimental period were below their maximum residue limits (MRL) for apple. The dissipation constants of acaricides in the apples were calculated at 0.0788 for etoxazole and 0.0319 for flufenoxuron corresponding to their biological half-lives; 8.8~21.7 days for etoxazole and 21.7~23.1 days for flufenoxuron. The PHRLs of acaricides in the apple showed the residual amounts of etoxazole and flufenoxuron at the harvesting date would be below their MRLs if their residual amounts were less than 0.87 and 0.88 mg/kg, respectively, at 7 days prior to harvesting the apples.
Development of Analytical Method for Cymoxanil in Agricultural Commodities using HPLC/UVD
Kim, Ji-Yoon ; Kim, Hea-Na ; Kim, Ja-Young ; Kim, Jong Geol ; Ham, Hun-Ju ; Lee, Young-Deuk ; Hur, Jang-Hyun ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 69~78
DOI : 10.7585/kjps.2014.18.2.69
In the present study, we developed an official individual analytical method for cymoxanil using HPLC/UVD, respectively in different representative crops. Individual analytical methods for these pesticides are not included in the Korea food code. The samples were extracted with acetonitrile, concentrated and partitioned with dichloromethane and saturated sodium chloride solution. For cymoxanil, extracts were concentrated and clean-up through silica gel column chromatography with dicloromethane/acetone (60/40 v/v) and subjected to instrumental analysis. The limit of detection (LOD) for cymoxanil were 0.1 ng and 1 ng respectively and limit of quantitation (LOQ) were 0.02 mg/kg. Recoveries for cymoxail ranged from 79.6~107.6% respectively, at fortification level of 0.02 mg/kg (LOQ), 0.2 mg/kg (10 LOQ) and 1.0 mg/kg (50 LOQ) and the coefficient of variation (CV) was less than 10%, regardless of sample types. These results were further confirmed with LC/MS. The proposed simultaneous analysis method is reproducible and sensitive enough to determine the residues of cymoxanil in the agricultural commodities. According to the validation data and performance characteristics and high sample throughput, the proposed method is suitable for routine application.
Development of an Analytical Method for the Determination of Pyriofenone residue in Agricultural Products using HPLC-UVD
Park, Hyejin ; Kim, HeeJung ; Do, Jung-Ah ; Kwon, Ji-Eun ; Yoon, Ji-Young ; Lee, Ji-Young ; Chang, Moon-Ik ; Rhee, Gyu-Seek ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 79~87
DOI : 10.7585/kjps.2014.18.2.79
Pyriofenone is an aryl phenyl ketone fungicide that is newly registered in Korea in 2013 to control powdery mildew on food. The objective of this study was to develop reliable and sensitive analytical method for determination of pyriofenone residue in agricultural products for ensuring the food safety. The pyriofenone residues in all samples(Korean melon, pepper, potato, mandarin, soybean, and hulled rice) were extracted with acetonitrile, partitioned with dichloromethane, and then purified with a silica cartridge. The purified samples were analyzed by HPLC-UVD and confirmed with LC-MS. The linear range of pyriofenone was 0.05~5 mg/kg with the correlation coefficient (
) > 0.999. Average recoveries of pyriofenone ranged from 72.8% to 99.5% at the spiked level of 0.05 and 0.5 mg/kg, while the relative standard deviation was 2.3%~6.4%. In addition, the limit of detection and limit of quantification were 0.01 and 0.05 mg/kg, respectively. The results revealed that the developed and validated analytical method was suitable for pyriofenone determination in agricultural products.
Assessment of Pre-Harvest Environmental Factors in Domestic Production of Organic Lettuce
Namgung, Min ; Kim, Beom Seok ; Heo, Seong Jin ; Choi, Yong Beom ; Hur, Jang Hyun ; Park, Duck Hwan ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 88~94
DOI : 10.7585/kjps.2014.18.2.88
Among pre-harvest environmental factors, increasing attention has been paid to the effects of chemical and microbiological factors on fresh produce. The occurrence and prevalence of these factors have been usually studied with regard to the final products at the post-harvesting stage and/or when they are sold in the market. However, the origin and routes of transmission of both factors remain to be clarified. In the present study, we examined the contamination levels of food-borne pathogens and chemical factors such as pesticide residues and heavy metals in 83 and 43 samples, respectively, including various soil, water, and fertilizer samples, as well as post-harvested and processed samples. Among the organic farming samples, only one pesticide, dimethomorph, was detected in the soil sample, however no pesticides were observed from any other samples in organic farming system. Thus, it was thought that might be contaminated from conventional farm land in the vicinity. Whereas many pesticide residues were detected in conventional farming systems such as soil, fertilizer, water, and fresh produce as expected. Furthermore, heavy metals detected from all tested samples did not shown contamination levels higher than the standard limit. We comparatively assessed the levels of contamination by food-borne pathogens on the samples from organic and conventional farming systems, and found aerobic bacteria at approximately 7 log CFU/g, with no significant differences observed between the two systems. Coliforms were present at lower levels than aerobic bacteria. No human pathogens were present among the coliforms detected, indicating that these bacteria are saprophytes without the ability to cause food-borne illnesses. In contrast, among the high-risk food-borne pathogens, only sporadic cells of Bacillus cereus were found on samples of organic farming system. These data extend previous findings that the most prevalent food-borne pathogen is B. cereus and demonstrate that it spreads to whole living plants via soil.
Acute and Chronic Exposure Assessment of Organophosphate Pesticides through the Consumption of Fruit Vegetables
Park, Byung-Jun ; Gil, Keun-Hwan ; Son, Kyeong-Ae ; Im, Geon-Jae ; Yoon, Hyunjoo ; Park, Kyung-Hun ; Kim, Doo-Ho ; Ihm, Yangbin ; Paik, Min-Kyoung ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 95~103
DOI : 10.7585/kjps.2014.18.2.95
In this study, we monitored the residues of organophosphate pesticides (OP) in eight fruit vegetables grown in Korea, and assessed risk levels of acute and chronic exposure of OP through the consumption of fruit vegetables. Chlorpyrifos, EPN, methidathion and phosphamidon in eight fruit vegetables were detected in this study. The results of cumulative assessment of national estimated short term intake for acute exposure of OP were due to the following order; eggplant, tomato, squash and cucumber. Total theoretical maximum daily intake calculated at first step of chronic exposure assessment for registered OP in Korea was 76.14%, compared with acceptable daily intake ADI) based on chlorpyrifos. In addition, total national estimated maximum daily intake calculated at second step of chronic exposure assessment was 13.949%, compared with ADI. Third chronic assessment was conducted by probabilistic approach using OP residues detected in eight fruit vegetables and showed that total exposure risk was very low, corresponding to 0.0001% compared to ADI. Based on those finding, the risk of organophosphate pesticides in fruit vegetables was considered quantitatively negligible. In future, further investigation to expand the target should be followed to do more accurate and detailed risk assessment.
Insecticidal Activity of Chinese Honeysuckle, Quisqualis indica Extracts against Scale Insects
Song, Jin Sun ; Lee, Chae Min ; Choi, Young Hwa ; Lee, Dong Woon ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 104~114
DOI : 10.7585/kjps.2014.18.2.104
Scale insects heavily damaged many agricultural crops and trees in urban and forest areas. This study was conducted to carry out alternative control methods for environmentally friendly control of scale insects. Hot water extract of Quisquali indica produced high mortality against the black pine bast scale, Matsucoccus thunbergianae in previously experiment. Methanol extract obtained from fruit of Q. indica was successively fractionated using hexane, chloroform, ethyl acetate, buthanol and water which were examined by exposing for their insecticidal activity against four Cocoidea species (Eriococcus lagerstroemiae, Ceroplastes japonicus, Crisicoccus pini and Planococcus citri) in laboratory. Hexane fraction gave the highest insecticidal activity against scale insect pest. GC-mass analysis confirmed that the main compounds of Q. indica were alpha-pinene, methyl palmitate, eugenol, methyl myristate, phthalic acid mono(2-ethylhexyl)ester and palmitic acid. Among those compounds,
-pinene was included 10 hexane subfractions.
-pinene was 0.0114 ppm against 2nd instar of E. lagerstroemiae. These results suggest that Q. indica extracts might be used as an alternative control agent of scale insects.
In vitro Fruit Assay for the Evaluation of Fungicide Activity Against Pepper Anthracnose
Lee, Soo Min ; Jang, Ho Seon ; Kim, Heung Tae ;
The Korean Journal of Pesticide Science, volume 18, issue 2, 2014, Pages 115~121
DOI : 10.7585/kjps.2014.18.2.115
This study was performed to investigate the effect of conidial density, wetness period and temperature on conidial germination, appressoria formation and disease incidence. While there was not significantly correlated between conidial density and temperature, and conidial germination and appressoria formation, there was a significant correlation between those factors and disease incidence. The longer wetness period was, the higher the ratio of conidial germination, appressoria formation and the disease incidence was. The optimum conidial density, temperature and wetness period was
and 5 days, respectively. In case the wetness period was more than 5 days, the typical symptom was not found on pepper fruits because of the overgrowth of mycelia. Using this fruit assay method, which the pepper anthracnose pathogens were inoculated by spraying spore suspension on non-wounded or wounded pepper fruits, control effect of three fungicides were evaluated against pepper anthracnose by the protective and/or the curative application. Propineb showed high protective control activity, while it showed curative control activity on unwounded fruits, but did not showed curative control activity on wounded fruits. Tebuconazole, one of curative fungicide, showed higher control activity in non-wound inoculation than wound inoculation. Trifloxystrobin, one of strobilurin group, showed high both protective and control activity against anthracnose. In conclusion, we supposed that the newly developed in vitro pepper fruit assay can be used to evaluate antifungal activity of control agents against pepper anthracnose.