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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 19, Issue 4 - Dec 2015
Volume 19, Issue 3 - Sep 2015
Volume 19, Issue 2 - Jun 2015
Volume 19, Issue 1 - Mar 2015
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Sample Preparation Method for Perfluorochemicals with LC-Tandem Mass Spectrometry in Agricultural Water
Kim, Jin Hyo ; Jin, Cho-Long ; Choi, Geun-Hyoung ; Park, Byung-Jun ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 1~4
DOI : 10.7585/kjps.2015.19.1.1
The current official perfluorochemicals (PFCs) analysis method is established with hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) clean-up method with LC-tandem mass spectrometry (
). Herein the HLB clean-up method from the official method by Korean government was modified with Envi-Carb
to improve the clean-up efficiency for agricultural water and the unexpected UV disturbance in the water was effectively removed by the adsorbent treatment without a significant disturbance of quantitation. And quadrupole time of flight mass spectrometry (qTOFMS) was suggested to the available
instruments for the residue analysis of PFCs based on the ng/L of quantitation limits in water as well.
Development of an Official Analytical Method for Determination of Imazapyr in Agricultural Commodities using HPLC-UVD
Jang, Jin ; Kim, Heejung ; Ko, Ah-Young ; Lee, Eun-Hyang ; Joo, Yoon Ji ; Kim, Jinhong ; Chang, Moon-Ik ; Rhee, Gyu-Seek ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 5~13
DOI : 10.7585/kjps.2015.19.1.5
A chromatographic method for the determination of imazapyr, a non-selective herbicide, in agricultural commodities was developed to use safety control of pesticide residue on crops, and was fully validated as an official method for residue analysis. Agricultural commodities, mandarin (fruit), hulled rice (cereal grains), pepper (vegetables), potato (potatoes) and soybean (beans) were extracted with methanol and partitioned with dichloromethane to remove the interference obtained from sample extracts, adjusting pH to 2.5 by 4N hydrochloric acid. Finally, they were analyzed by high performance liquid chromatography coupled to UV detector (HPLC-UVD). The developed method had the linearity in the range of test concentrations with coefficients of determination (
) more than 0.99. Recovery studies were carried out at three concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates at each level. Recoveries were ranged between 72.1 to 108.0%, with relative standard deviations less than 10%. A consistent recovery was determined according to the CODEX guidelines (CAC/GL40, 2003). Finally, LC/MS with selected ion monitoring was also applied to confirm the suspected residues of imazapyr in agricultural samples. This developed method for determination of imazapyr residues in agricultural commodities. can be used as an official method.
Residual Characteristics and Behavior of Azoxystrobin in Ginseng by Cultivation Conditions
Lee, Jae Yun ; Noh, Hyun Ho ; Park, Hyo Kyoung ; Kim, Jin Chan ; Jeong, Hye Rim ; Jin, Me Jee ; Kyung, Kee Sung ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 14~21
DOI : 10.7585/kjps.2015.19.1.14
To determine residual characteristics of azoxystrobin in ginseng under different cultivation conditions such as use of straw mat on cultivation soil and filling gap between ginseng stem and soil surface and also to elucidate its approximate behavior after spraying, 20% azoxystrobin suspension concentrate solution was sprayed 4 times onto 5-year-old ginseng with 10 days interval at a application rate of about 200 L/10 a and then residues in samples were analyzed. The residue level was lower in case of use of straw mat and filling the gap with soil than in case of no use of straw mat and no filling the gap, representing that use of straw mat and filling the gap with soil were contributed to decrease of pesticide residues in ginseng. A large portion of the test pesticide distributed onto ginseng leaf with a higher specific surface area. The amounts of azoxystrobin residues decreased in ginseng leaf, while increased on soil surface, as close to harvest. About 0.1% of azoxystrobin sprayed was distributed in ginseng root and 12.7-20.4% (mean 16.6%) of azoxystrobin could be decreased for dietary intake by removing of rhizome from ginseng root before intake.
Monitoring and Safety Assessment of Pesticide Residues on Agricultural Products Sold via Online Websites
Park, Duck Woong ; Kim, Ae Gyeong ; Kim, Tae Sun ; Yang, Yong Shik ; Kim, Gwang Gon ; Chang, Gil Sik ; Ha, Dong Ryong ; Kim, Eun Sun ; Cho, Bae Sik ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 22~31
DOI : 10.7585/kjps.2015.19.1.22
This study was carried out to monitor the current status of pesticide residues in selling agricultural products via online and assessed their safety in 2014. A total of 124 samples were purchased six times from March to August 2014 twenty online shopping malls randomly. These samples were analysed 208 pesticides by multiresidue method using a GC-ECD/NPD and a LC-MS/MS and confirmed by a GC-MSD. As a result of analysis, residual pesticides samples were 11 (8.9%) such as leek, young radish, welsh onion etc, of which 2 samples (1.6%) such as sesame bud (Chlorothalonil), artemisia (Chlorpyrifos) were violated Korea Maximum Residue limits (MRLs). 11 kinds of pesticides (19 times) were detected in 11 samples. Risk assessment evaluated human health exposure with the ratio of EDI (Estimated daily intake) to ADI (Acceptable daily intake) of pesticides detected. %ADI (the ratios of EDI to ADI) were 0.04~95.70% and some samples represented a fairly dangerous levels. In particular, Chlorothalonil in the sesame bud was shown as a significant risk close to 100% of %ADI. Accordingly, it is recommended to strengthen a safety check on agricultural products in online sales.
Monitoring and Exposure Assessment of Pesticide Residues in Domestic Agricultural Products
Kang, Namsuk ; Kim, Seongcheol ; Kang, Yoonjung ; Kim, Dohyeong ; Jang, Jinwook ; Won, Sera ; Hyun, Jaehee ; Kim, Dongeon ; Jeong, Il-Yong ; Rhee, Gyuseek ; Shin, Yeongmin ; Joung, Dong Yun ; Kim, Sang Yub ; Park, Juyoung ; Kwon, Kisung ; Ji, Youngae ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 32~40
DOI : 10.7585/kjps.2015.19.1.32
This study was implemented to evaluate food safety on residual pesticides in agricultural products of Korea and to use as a data base for the establishment of food policy. A total of 196 pesticide upon these products were analyzed using multi class pesticide multiresidue methods of Korean Food Code, and 232 samples of 15 agricultural products collected from 9 regions were supplied for this study. In the results, 64 kinds of pesticides were detected in 53 samples, chlorpyrifos and procymidone of them were shown a high frequency of detection in the analyzed pesticides. Among them, two samples (chlorpyrifos in perilla leaves and picoxystrobin in peach) were detected over Maximum Residue Limits (MRLs). The levels of the detected pesticide residues were within safe levels. Also, the intake assessment for pesticide residues including chlorpyrifos at multi pesticide residue monitoring were carried out. The result showed that the ratio of EDI (estimated daily intake) to ADI (acceptable daily intake) was 0.001~0.902% which means that the detected pesticide residues were in a safe range so that residual pesticides in the agricultural products in Korea are properly controlled.
Evaluation of Exposure to Pyrethroid Pesticides in Highland Cabbage Farmers by Using Biological Monitoring
Km, Ha Kyoung ; Song, Jae Seok ; Choi, Hong Soon ; Yu, Ho Young ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 41~46
DOI : 10.7585/kjps.2015.19.1.41
This study was conducted to evaluate the use of biological monitoring to determine the factors that influence the effects of exposure to pyrethroid pesticides among highland cabbage farmers. Urine was collected from farmers who was cultivating Chinese cabbage in the relatively highground in Gangwon-do the morning following pesticide application and was analyzed for cis- and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcy-clopropane carboxylic acid (DCCA) and 3-(2,2-dibromovinyl)-2,2-dimethyl cyclopropane carboxylic acid (DBCA). Factors affecting exposure to pesticides, such as wind speed, spraying location, and the use of protective gear, were also investigated. Statistical analysis was performed by the Kruskal-Wallis test. Our analysis indicated that highland cabbage farmers were exposed to a higher level of pesticides than the general public or other types of farmers. When the wind speed was low, workers who sprayed pesticides were exposed to a higher level of pesticides compared to the exposure level in an assistant. However, there was no difference in exposure between the two when wind speed was high.
Evaluation of Antimicrobial Activity and Disease Control Efficacy of Sodium Dichloroisocyanurate (NaDCC) Against Major Strawberry Diseases
Kim, Da-Ran ; Gang, Gun-Hye ; Cho, Hyun-Ji ; Yoon, Hae-Suk ; Kwak, Youn-Sig ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 47~53
DOI : 10.7585/kjps.2015.19.1.47
Various diseases occur in fruits, leave and roots during strawberry cultivation and cause severe economical damage and huge amount of chemical fungicide use. Recently, as consumers' interest in safety of foods and organic agriculture produces have increased, control measures using alternatives for chemical fungicides have been newly developed in various ways. This study was conducted to test antifungal activity and control effect of sodium dichloroisocyanurate (NaDCC), using as disinfectants, against major disease pathogens of strawberry, Fusarium oxysporum (Fusarium wilt), Colletotrichum gloeosporioides (Anthracnose) and Phytophthora sp. (Phytophthora blight), and Xanthomonas fragariae (bacterial angular leaf spot) and evaluate availability as environment-friendly materials. When NaDCC was treated at the concentration range of 150 to 300 ppm, it suppressed significantly hyphal growth and reduced spore germination by more than 28%. In field condition, NaDCC showed excellent control effect (control value: 50%) against the bacterial angular leaf spot disease. Based on above-described results, we suggested that NaDCC can be used as alternative candidates to chemical pesticide alternatives of for controlling strawberry diseases.
Investigation of Fungicide Response of Streptomyces spp. Isolated from Rhizosphere in Zoysiagrass
Lee, Jung Han ; Min, Gyu Young ; Jeon, Chang Wook ; Choi, Su Min ; Shim, Gyu Yul ; Kwak, Youn-Sig ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 54~63
DOI : 10.7585/kjps.2015.19.1.54
Streptomyces spp. isolated from turfgrass rhizosphere and tested for their response to large-patch control fungicides. The tested fungicides were actually used in golf course or turfgrass cultivation to prevent large-patch disease. Tolerance to 3 triazole group of the strains was the highest to the PR fungicide, and following the SR fungicide, whereas the isolated strains were no tolerance to HR fungicide. Tolerances to three kind of Strobilurin group were similar for the all of the tested Streptomyces spp.. Growth and sporulation of the all strain was normal in CB and AP fungicide treatments. However no spore formulated in double concentration. Strains, tolerance to acetanilide fungicides, appeared that KT fungicide tolerance was higher than MK fungicide. The selected strains showed strong tolerance against AT fungicide but have no tolerance to ATR fungicides. In conclusion, the bacterial strains showed tolerance against 1 carbamate, 1 organophosphate and 1 cyanopyrrole group, while have no tolerance against two mixture formulations (1 Quinone + Strobilurin and 1 Imidazole + Triazole).
Control Activities of Fungicides Against Garlic White Rot Caused by Sclerotium cepivorum
Kim, Heongjo ; Kim, Heung Tae ; Min, Yi Gi ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 64~70
DOI : 10.7585/kjps.2015.19.1.64
In order to control garlic white rot (Sclerotium cepivorum), which threatens garlic production in farmers fields, soil solarization (solar sterilization), sclerotia germination inducers and effective microorganisms as biological control agents, and chemical fungicides have been used. Among them, fungicide has been largely used to reduce garlic white rot. In this study, the antifungal activities of five fungicides, prochloraz(a.i. 25%, EC), tebuconazole (a.i. 25%, WP), flutolanil (a.i. 15%, EC), iminoctadine tris-albesilate (a.i. 40%, WP) and isoprothiolane (a.i. 40%, EC) with different mode of action, in mycelial growth, sclerotia germination and sclerotia production, were tested. The inhibitory effects of the 5 fungicides on the mycelial growth, and sclerotia germination and production of garlic white rot pathogen (S. cepivorum T11-2) were investigated on potato dextrose agar (PDA) and their control efficacies were evaluated on garlic flakes. There was no mycelial growth of S. cepivorum T11-2 on PDA amended with
of prochloraz or
of tebuconazole. Also prochloraz and tebuconazole inhibited perfectively the sclerotia germination of the pathogen at 10 and
, respectively. In spite of a very low activity of isoprothiolane in mycelial growth and sclerotia germination of S. cepivorum T11-2, it showed a good inhibitory activity against sclerotia production of S. cepivorum T11-2 on PDA amended with
. Prochloraz, tebuconazole and flutolanil showed above 70% of control value when they were treated at
using the garlic flake cutting-method.
Antifungfal Activity Against Plant Pathogenic Fungi on Insect Enterobacteriaceae
Oh, San Na ; Seo, Mi Ja ; Youn, Young Nam ; Yu, Yong Man ;
The Korean Journal of Pesticide Science, volume 19, issue 1, 2015, Pages 71~79
DOI : 10.7585/kjps.2015.19.1.71
In order to investigating the effects of antifungal activity of intestinal bacteria obtained from insect, it was identified these bacteria isolated from the gut. In this result, total 49 isolates of intestinal bacteria were identified from 10 kinds of insect species. It was that 4 isolates including Cedecea sp. from Nesidiocoris tenuis, 3 isolates including Enterobacter sp. from Odontotaenius disjunctus, 4 isolates including Acinetobacter sp. from Reticulitermes speratus, 4 isolates including Clavibacter sp. from Riptortus clavatus, 11 isolates including Bacillus sp. from Lema decempunctata, 3 isolates including Enterococcus sp. from Henosepilachna vigintioctopunctata 2 isolates including Staphylococccus sp. from Harmonia axyridis, 5 isolates including Enterobacter asburiae from Popillia mutans, 7 isolates including Aeromonas sp. from Hydrophilus acuminatus, and 7 isolates including Brucella sp. from Anomala octiescostata. In order to investigating antifungal activity against plant-pathogenic fungi, Altanaria solani, Colletotrichum gloeosporioides, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici, Rhizoctonia solani and Selerotinia sclerotiorum were dual cultured with each 49 gut enterobacteriaceae. As these results showed that many isolates have the antifungal activities including 26 isolates against A. solani, 6 isolates against B. cinerea, 13 isolates against C. gloeosporioides, 11 isolates against F. oxysporum, 17 isolates P. capsici, 2 isolates against R. solani and 2 isolates against S. sclerotiorum. Pseudomonas aeruginosa was showed strong antifungal activity against all of tested plant pathogens. It might be taken a potential for application against plant-pathogenic fungi with useful control agent.