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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
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Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 19, Issue 4 - Dec 2015
Volume 19, Issue 3 - Sep 2015
Volume 19, Issue 2 - Jun 2015
Volume 19, Issue 1 - Mar 2015
Selecting the target year
Development of Analytical Method for Fenoxanil in Agricultural Products Using GC-NPD and GC/MS
Kim, Gyeong-Ha ; Ahn, Kyung-Geun ; Kim, Gi-Ppeum ; Hwang, Young-Sun ; Lee, Young Deuk ; Choung, Myoung-Gun ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 345~353
DOI : 10.7585/kjps.2015.19.4.345
The aim of this study is to develop residue analysis method for fenoxanil, a MBI (melanin biosynthesis inhibitor) propionamide fungicide, had mainly been used to control rice blast, and disease of other crops, fruits, and vegetables by using GLC/NPD and GC/MS. Extraction with acetone and partition with n-hexane/dichloromethane (80/20, v/v) were performed from hulled rice, soybean, Kimchi cabbage, green pepper, and apple, then column clean-up with florisil was applied. Mean recoveries were 82.2%-109.1% with less than 7.2% of coefficients of variation and limit of quantitation was set at the concentration of 0.04 mg/kg from the five agricultural products through the determination by GLC/NPD equipped with DB-5 capillary column and single laboratory validation. As a confirmatory method, GC/MS selected ion monitoring (SIM) was set from m/z 125.0, 188.9, and 293.0. Developed method is expected to apply the single residue analysis of fenoxanil in agricultural products.
Residual characteristic of tebuconazole and fludioxonil in Allium victorialis (Allium ochotense Prokh.)
Woo, Min-Ji ; Hur, Kyung-Jin ; Kim, Ji-Yoon ; Saravanan, Manoharan ; Kim, Se-Weon ; Hur, Jang-Hyeon ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 354~360
DOI : 10.7585/kjps.2015.19.4.354
In recent years, Allium victorialis has been extensively used as a pharmacological agent for various diseases in the form of anti-arteriosclerotic, anti-diabetic and anti-cancer. Allium victorialis is severely affected by various fungal diseases since it naturally grow in the shady and humid environments in Korea. In this case, different types of fungicides are applied to control the fungal diseases in Allium victorialis. The present study was aimed to determine the residual characteristics of two fungicides namely tebuconazole and fludioxonil on Allium victorialis. For this study, the fungicides were drenched soil on Allium victorialis in the cultivation area Pyeongchang by the standard (two thousand fold) and double (thousand fold) dilutions. At the end of
days samples were collected for residue analysis. Residues of tebuconazole and fludioxonil were analyzed using GC/NPD (Gas Chromatography/Nitrogen Phosphorus Detector) and their recovery were found to be 108.8~119.5% and 91.3~104.8%, respectively. The method of limits of quantification for both fungicides was
. Further, the results of this study shows that the residue levels of both fungicides on Allium victorialis were <
and their % ADI (% Acceptable Daily Intake) were 17.44% and 25.75%, respectively. Based on the results obtained in this study, we suggest that the residue levels of both of the fungicides on Allium victorialis are safe and these fungicides can also be used to control fungal diseases in Allium victorialis.
Residual Characteristics of Bistrifluron and Fluopicolide in Korean Cabbage for Establishing Pre-Harvest Residue Limit
Lee, Dong Yeol ; Jeong, Dong Kyu ; Choi, Geun-Hyoung ; Lee, Deuk-Yeong ; Kang, Kyu Young ; Kim, Jin Hyo ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 361~369
DOI : 10.7585/kjps.2015.19.4.361
This study investigated residual characteristics of bistrifluron and fluopicolide in Korean cabbage, and suggested the pre-harvest residue limits (PHRLs) based on their dissipation patterns and biological half-lives. The pesticides were sprayed on Korean cabbage in two different region under greenhouse conditions at the recommended dose, respectively. The samples for residue analysis were harvested at 0 (2 hr), 1, 2, 4, 6, 8 and 10 days after treatment, and analyzed by HPLC after clean-up with Florisil SPE. The limit of quantification (LOQ) was
for bistrifluron and fluopicolide, and the recoveries ranged from 87.2-110.6% with below 5% of RSD. The biological half-lives of field I and field II were 3.9 and 4.2 days for bistrifluron and 4.9 and 4.2 days for fluopicolide, respectively. The PHRL of bistrifluron and fluopicolide were recommended as 3.83 and
for 10 days before harvest, respectively.
Comparative Study of the Efficiency of GC with Large Volume Injector and SPE Clean-up Process Applied in QuEChERS Method
Park, Young Jun ; Hong, Su Myeong ; Kim, Taek Kyum ; Kwon, Hye Young ; Hur, Jang Hyun ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 370~393
DOI : 10.7585/kjps.2015.19.4.370
This study was conducted to compare STQ method, multi-residue method in Korean food code and QuEChERS method for validated selected and accuracy, reproducibility and efficiency. A total of 45 selected and targeted pesticides were the analyzed by GC and 5 of them were crops (apple, potato, green pepper, rice, soy bean).
values were calculated in the standard calibration curve was over 0.990. Recovery tests were performed by three replications in two levels and the relative standard deviation of the repeated experiments was less than 30%. The average percentage of recoveries in the multi-residue method in Korean food code was 89.13%, QuEChERS method was 92.45% and STQ method was 85.28%. In addition, matrix effects in multi-residue method in Korean food code was 24.61%, QuEChERS method was 23.98% and STQ method showed 11.24%. The STQ method is easy and showed high clean-up effect in extracting the sample solution than the QuEChERS method and clean-up with C18, PLS, PSA cartridge columns. A large volume of the sample was injected in order to compensable for the problem, that occurred due to high detection limit in the analyser. When the STQ method was applied using a large volume injector, the standard calibration curve showed a higher linearity
, and method detection limit was 0.01 mg/kg. It showed an average recovery of 91.84% and the relative standard deviations of three replications repeated in two level process was less than 30% and had an average matrix effect of 17.90%.
Establishment of Economic Threshold Caused by Rice Sheath Blight Disease severity
Shim, Hongsik ; Choi, Hyo Won ; Yeh, Wan-Hae ; Lee, Yong-Hwan ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 394~398
DOI : 10.7585/kjps.2015.19.4.394
This research was performed to establish economic threshold (ET) for rice sheath blight disease with the cultivar Odeabyeo. Total yield and yield components, such as the panicle number per hill, the spikelet per panicle, the percent ripened grain and the thousand grain weight were evaluated depending on the disease severity of sheath blight on rice, respectively. Significant negative correlation between the percent of diseased hill (PDH) and total yield was observed (r = -0.93). Moreover, negative correlation coefficients were found between PDH and spikelet per panicle, and percent ripened grain (r = -0.66 and -0.77, respectively). There were no correlations between PDH and the panical number per hill, and a thousand grain weight, respectively. In this study, economic threshold level on sheath blight disease on rice was established on 7.8% of PDH.
Promotive Effects of Geraniol on Radicle Growth of Several Vegetables and Leaf Growth of Brassica campestris
Choi, Geun-Hyoung ; Jeong, Dong-Kyu ; Park, Byung-Jun ; Cho, Nam-Jun ; Hong, Jin-Hwan ; Kim, Jin-Hyo ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 399~401
DOI : 10.7585/kjps.2015.19.4.399
This study investigated the plant growth promoting activity of geraniol on Chinese cabbage (Brasscia campestris ssp. Pekinensis Rupr.), Pak Choi (Brassica rapa L.), Crown daisy (Chrysanthemum coronarium L.), Iceberg lettuce (Lactuca sativa L.) and Leaf perilla (Perilla frutescens Britt.). Geraniol at 0.5 to
promoted radical growth of Chinese cabbage Pak Choi, Crown daisy, Iceberg lettuce and leaf perilla by >30%. In the pot experiments, geraniol treatment at
by drenching increased up to 190% of the aerial part growth promotion. By comparison, foliar application showed 124% promoting activity only at
Estimation of the Chitinolytic and Antifungal Activity of Streptomyces sp. CA-23 and AA-65 isolates Isolated from Waste Mushroom Media
Shim, Chang-Ki ; Kim, Min-Jeong ; Kim, Yong-Ki ; Jee, Hyeong-Jin ; Hong, Sung-Jun ; Park, Jong-Ho ; Han, Eun-Jung ; Kim, Seuk-Chul ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 402~410
DOI : 10.7585/kjps.2015.19.4.402
The purpose of this study was to estimate the chitinolytic and antifungal activity of Actinomycetes sp.isolated from waste mushroom media. In five kinds of waste mushroom media, Sinyeong mushroom and Yangsongi were the order of the population density of actinomycetes. Totally 91 chitinolytic isolates of Actinomycetes sp. were obtained from waste mushroom media. The isolates were categorized into 3 groups based on chitinolytic activity and antagonisms against Phytophthora capsici, Rhizoctonia solani, Sclerotinia sclerotiorum, Collectotrichum gloeosporioides, and Cladosporium cucumerinum in vitro. CA-23 was selected as a representative isolate of a group showing strong chitinolytic and antagonistic activities to all of the plant pathogens, while AA-65 was selected as a representative isolate showing no chitinolytic activities but strong antagonistic activities to the pathogens. CA-23 and AA-65 were highly effective on control of Phytophthora blight of hot-pepper, powdery mildew and scab of cucumber in a greenhouse tests. Among the isolates tested, CA-23 showed highest control efficacy, while AA-65 not only effectively controlled the diseases but also consistently increased plant growth and yield. Although the isolates are similarly affected on suppression of plant pathogens, the isolates could be differ from each other in modes of action. Further studies on mechanisms and practical applications are being progressed.
Combination effects of Organic Materials and Bacillus thuringiensis on Spodoptera exigua
Han, Ji Hee ; Yoon, Jihye ; Son, Sujin ; Kim, Jeong Jun ; Lee, SangYeob ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 411~417
DOI : 10.7585/kjps.2015.19.4.411
This study is conduced to investigate control efficacy of mixture of plant extracts which has been reported to control pest and Bacillus thuringiensis or alone. Control efficacy of 0.025% matrine or mixture of 0.016% matrine and BT (
instar larvae of beet armyworm were best as 98.7% and 93.3%. Treatment of mixture of 0.01% matrine and BT (
) showed the mortality of 69.3%. In the case of neem, when the mixture of 0.1% neem and BT was applied to larvae of S. exigua, their mortality was 50.0% and weight of larvae were significantly different compare with control and only neem treatment. Therefore For effective control reducing population of beet armyworm and possibility to develop resistance against BT and eco-friendly agents, mixture of 0.016% Matrine and BT (
) could be used.
Susceptibility of the Predatory Mite, Neoseiulus californicus (Acari: Phytoseiidae) to Acaricides
Lee, Sung Min ; Kim, Sang Soo ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 418~423
DOI : 10.7585/kjps.2015.19.4.418
Effects of 9 acaricides to the predatory mite, Neoseiulus californicus were evaluated. Seven of the acaricides tested, cyenopyrafen. spirodiclofen, spiromesifen, acequinocyl, bifenazate, flufenoxuron and cyflumetofen exhibited low toxicity to adult females and nymphs of N. californicus and had little effect on the reproduction and eclosion of eggs deposited by treated predators. Moreover, hatch percentage of N. californicus eggs was unaffected by exposure to these seven acaricides. Etoxazole did not significantly affect the survival and reproduction of adult female predators but caused very low eclosion in eggs laid by treated females and high egg mortality. Pyraclofos was extremely toxic to adult female predators and caused 100% mortality. Adult female predators survived on a diet of spider mites treated with cyenopyrafen. spirodiclofen, spiromesifen, acequinocyl, bifenazate, flufenoxuron and cyflumetofen and their fecundity was not substantially affected. Based on the results, cyenopyrafen. spirodiclofen, spiromesifen, acequinocyl, bifenazate, flufenoxuron and cyflumetofen are appeared to be promising candidates for use in integrated mite management program where N. californicus is the major natural enemy.
Screening of Seed Treatment Fungicide for Control of Damping-off caused by Rhizoctonia solani on Panax ginseng
Shin, Jeong-Seop ; Lee, Seung-Hwan ; Cho, Hye-Sun ; Cho, Dae-Hui ; Kim, Kyoung-Ju ; Hong, Tae-Kyun ; Park, Chol-Soo ; Lee, Seong-Kye ; Jung, Hee-Young ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 424~427
DOI : 10.7585/kjps.2015.19.4.424
Damping-off is a critical disease on ginseng seedling, which caused by the fungal pathogen Rhizoctonia solani. The disease has been prevented by tolclofos-methyl for the last 20 years. However, the tolclofos-methyl usually detected on the harvested roots of 6-year-old ginseng. Herein, we tried to select an alternative pesticide which not only must be safe but also efficiently inhibits the fungal pathogen. Four fungicides (fludioxonil, flutolanil, pencycuron, and thifluzamide) were applied to their inhibition efficacy against the pathogen. In in vitro test, fludioxonil treatment showed 80% inhibition activity for 25 days. Thifluzamide and flutolanil showed the activity for 10 days. Pencycuron showed for 1 days. In addition, the fludioxonil was more effective to control the pathogen comparing to other three fungicides in field. The incidence of damping-off was reduced to 71% by fludioxonil treatment. The level of the fungicide residue in seedling was 0.44 mg/kg, which value will be a negligible level in final products after 5 years. Consequently, the fludioxonil is a conceivable alternative for tolclofos-methyl to cope with R. solani.
Control of Pepper Anthracnose Caused by Colletotrichum acutatum using Alternate Application of Agricultural Organic Materials and Iminoctadine tris + thiram
Hong, Sung-Jun ; Kim, Yong-Ki ; Jee, Hyeong-Jin ; Shim, Chang-Ki ; Kim, Min-Jeong ; Park, Jong-Ho ; Han, Eun-Jung ; Kim, Jung-Hyun ; Kim, Seok-Cheol ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 428~439
DOI : 10.7585/kjps.2015.19.4.428
Pepper anthracnose caused by Collectotrichum acutaum has been known as one of the most damaging diseases of pepper, which has reduced not only yield but also quality of pepper produce almost every year. This study was conducted to develop control strategy against pepper anthracnose by alternate application of agricultural organic materials and chemical fungicides. The alternate application effect of agricultural organic materials and chemical fungicides for controlling pepper anthracnose were examined in vitro and in the field. First, thirteen microbial agents and twenty two agricultural organic materials were screened for antifungal activity against C. acutatum through the dual culture method and bioassay. As a result, one microbial agent (Bacillus subtilis QST-713) and three agricultural organic materials (sulfur, bordeaux mixture, marine algae extracts) were found to show high inhibition effect against C. acutatum. In the field test, when Iminoctadine tris+thiram, a chemical fungicide for controlling pepper anthracnose, was sprayed, it reduced disease incidence by 89.5%. Meanwhile Sulfur, bordeaux mixture, copper, marine algae extracts and Bacillus subtilis QST-713 showed low disease incidence at the range of 33.1~81.0%. However, when Iminoctadine tris+thiram and agricultural organic materials(bordeaux mixture, marine algae extracts) were applied to pepper fruits alternately two times at 7 days interval, there was a 81.7 and 87.1% reduction in disease, respectively. Consequently, the alternate spray of chemical fungicide (Iminoctadine tris+thiram) and agricultural organic materials (bordeaux mixture, marine algae extracts) could be recommended as a control method to reduce the using amount of chemical fungicide.
Development of Effective Screening Method for Efficacy Test of Trunk Injection Agents Against Pine Wood Nematode, Bersaphelenchus xylophilus in Japanese Black Pine, Pinus thunbergii
Shin, Won Sik ; Jung, Young Hack ; Lee, Sang Myeong ; Lee, Chae Min ; Lee, Chang Joon ; Kim, Dong Soo ; Mun, Il Sung ; Lee, Dong Woon ;
The Korean Journal of Pesticide Science, volume 19, issue 4, 2015, Pages 440~449
DOI : 10.7585/kjps.2015.19.4.440
Pine wood nematode (PWN), Bursaphelenchus xylophilus is one of the most serious pests of pine tree. Trunk injection of some nematicides in tree is well known as an effective control method. However there are some limitating factors which hindering the efficacy of trunk injection in field or potted tree. In this study we suggested easy and useful alternative screening methods of nematicides against PWN. Reproduction of PWN was influenced by tree twig moisture (high reproduction in high moisture trig) and paraffin coating was 78.6% reduced moisture loss in tested twig. There were no reproduction different in up and down site from infection site of twig at 1 month after inoculation of PWN and also distance (5 and 10 cm) from inoculation site of PWN did not influence the reproduction of PWN. Numbers of reproduced PWN were higher with decreasing diameter of twig. Numbers of reproduced PWN were similar to P. thunbergii and P. densiflora. However reproduction was increased depending on high inoculation density and longer propagation period. When inoculation of PWN on cut twig injected with emamectin benzoate 2.15% EC and morantel tartrate 8% SL in trunk of Pinus thunbergii in the field, PWN number were significantly reduced than untreated control. We suggest this screening method for PWN control agents.