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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
Editor in Chief :
Volume & Issues
Volume 4, Issue 4 - Dec 2000
Volume 4, Issue 3 - Sep 2000
Volume 4, Issue 2 - Jun 2000
Volume 4, Issue 1 - Mar 2000
Selecting the target year
Analysis of some pesticides in urine by GC/MS
Park, Seong-Soo ; Park, Song-Ja ; Pyo, Hee-Soo ; Cho, Jung-Hee ; Kim, Hye-Soo ; Park, Taek-Kyu ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 1~10
The purpose of this study is to establish the assessment techniques of hazardous chemicals by the development of analytical method of biological samples. In this study, we have developed an extraction method of nine pesticides used for rice paddy that resulted in high recovery from the spiked human urine by the liquid-liquid extraction with diethyl ether at pH 7.0. Calibration curve obtained from each pesticide standard using by gas chromatography/mass spectrometry/selected ion monitoring has shown good linearity and detection limits were the range of
ng/mL in urine. As a biological monitoring, urine samples of local farmers exposed directly to nine pesticides in the field were collected and analyzed by GC/MS. Of the tested pesticides, metabolites of phenthoate assumed were identified by GC/MS analysis. No parent compound was detected.
Spectrophotometric microtitre assay for rapid screening of membrane-disrupting herbicides
Kwon, Ok-Kyung ; Cho, Kwang-Yun ; Kim, Jin-Seog ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 11~18
This study was conducted to establish an efficient screening method of membrane-disrupting herbicides, based on the result that membrane leakage leading to evolution of carbonyl compounds and aldehydes could be colorimetrically assessed by formation of stable chromophores with MBTH and ferric chloride. Assay conditions including plant material, light intensity, irradiation time, concentrations of reagents for colorization, and reaction time were optimized, and activities of different types of known herbicides were investigated with cucumber cotyledon discs. That result indicated that this assay was sensitive to membrane-disrupting herbicides, such as protoporphyrinogen oxidase inhibitors (acifluorfen, oxyfluorfen, oxadiazon) and an electron interceptor from photosystem I(paraquat). With seven compounds newly synthesized, highly positive correlation was observed between herbicidal activities measured by greenhouse bioassay and MBTH method. Because only a single disc(4 mm diameter) from cucumber cotyledon in 60
of incubation solution was required to assay, a large number of herbicides could be tested simultaneously using 96-well microtitre plates. But the assay efficiency is not likely to significantly increase because of the boiling step during the reaction.
Adsorption and residues of EPN in the soil of Cheju Island
Kim, Jung-Ho ; Kam, Sang-Kyu ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 19~25
The adsorption and leaching of organophosphorus pesticide, EPN (O-ethyl-O-4 -nitrophenyl phenyl phosphonothioate) were investigated in Namwon soil(black volcanic soil), Aewol soil(very dark brown volcanic soil) and Mureung soil(dark brown nonvolcanic soil) in Cheju Island. The residue of EPN was surveyed on coastal environment of Cheju in Aug. 1996. The organic matter of Namwon soil, Aewol soil and Mureung soil was 19.8, 6.2 and 2.4%, respectively. The cation exchange capacity of Namwon soil, Aewol soil and Mureung soil was 24.8, 13.0 and 9.5 meq/100 g, respectively. The Freundlich constant, k value, was 89.4, 26.9 and 9.25 for Namwon soil, Aewol soil and Mureung soil, respectively. The k value of Namwon soil with very high organic matter content and cation exchange capacity was the highest for Aewol soil and Mureung soil. The Freundlich constant, 1/n, show a high correlation with organic matter content, i.e. it is less than unity for organic matter rich soil of Namwon soil and greater than unity for organic matter poor soil of Mureung soil. The leaching of EPN was slower for Namwon soil with high k values, and faster for Mureung soil with low k values. The results of the study was demonstrated the potential of pollution for EPN have little leached into soil environment. EPN was not detected in seawater and sediment in the coastal environment in Cheju Island. EPN used in the farm on tile Cheju island were not residued in the coastal environment.
An extraction method and residues of benzimidazole fungicides in soybean sprouts
Park, Jun-Jo ; Yong, Kum-Chan ; Jeung, Jin-A ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 26~31
An analytical method was approached to determine the residues of benzimidazole fungicides in soybean sprouts and was compared with traditional solvent-based method of extraction, solid phase extraction(SPE) and matrix solid phase dispersion(MSPD). The recoveries of carbendazim for liquid-liquid extraction (LLE), SPE and MSPD in soybean sprouts were
, respectively. The recoveries of thiabendazole for LLE, SPE and MSPD in soybean sprouts were
, respectively. The residues of carbendazim by elapsed time under our cultivated soybean sprouts program found
mg/kg after 5 days and trace concentration of carbendazim was detected after 7 days. Of the 448 samples analyzed, less than 1.5% had residues of carbendazim ranged from 0.05 to 0.37 mg/kg.
Biological activities of some organometalic compounds as artificial nuclease
Sung, Nack-Do ; Kim, Dae-Whang ; Kwon, Byung-Mok ; Kim, Tae-Young ; Suh, Il-Hwan ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 32~37
A series of transition metal complexes of 3,6-bis(6'-methyl-2'-pyridyl)pyridazine (
) and 3,6-bis(2'-pyridyl)pyridazine (
) as artificial nuclease,
were synthesized. After determining of X-ray crystal structure, hydrolysis rate constants of phosphates, as DNA model compound and biological activities were confirmed.
-Zn(II) complex, 8 was shown the best hydrolysis rate constant. The
-Ni(II) complex, 5 and
-Co(II) complex, 6 showed the highest herbicidal activity against SCP (Scriptus Juncoids) with excellent tolerance to rice, ORY (Oryzae sativa L.). And the
-Co(II) complex, 2,
-Zn(II) complex, 4 and ligand (
) displayed above 90% fungicidal activity against MAG (Magnaporthe grisea).
The present knowledge of farmers on pests and chemical control in grapevine culture
Cha, Byeong-Jin ; Lee, Yun-Sang ; Lee, Hyung-Rae ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 38~43
As the first step of a research on the establishment of control strategy for grapevine diseases and insects, the current status of pest control and yield losses by them were surveyed from grapevine growers of Korea. For insects, the most difficult to control was a grapevine stem borer and a grapevine clearwing moth was the next. On the other hand, several diseases including anthracnose, downy mildew, powdery mildew, bird's eye rot, leaf blight, were answered by the growers. The occurrence of the pests varied to the cultural practices. Yield loss was more serious in rainfall intercept culture than in plastic film house culture and diseases caused more loss in yield than insects did. However, the grapevine grower's potential for the identification of the kinds of pests was not high enough. When the pest was observed, the growers consulted mainly with local pesticide dealers or neighbouring growers and usually applied the pesticides prescribed by the dealer. More than half of the growers did not use pest control calendar, and most of the growers applied pesticides before any symptom appeared. Also, more than half of the growers applied mixture of at least more than 1 kind of pesticide and nutrients. Insecticides were applied less than 5 times during the season, but it was more than 6 times for fungicides. In the pesticide selection, the growers checked control effect first, regardless of the registration, and the pest control cost per unit area varied very much depending on the growers.
Penetrations of flupyrazofos against Plutella xylostella(Lepidoptera :Yponomeutidae) and Spodoptera exigua(Lepidoptera : Noctuidae)
Lee, Sang-Guei ; Hwang, Chang-Yeon ; Han, Man-Jong ; Yoo, Jai-Ki ; Lee, Hoi-Seon ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 44~50
Tolerance mechanism to flupyrazofos was examined with Plutella xylostella (L.) and Spodoptera exigua by investigating the penetration rate of flupyrazofos into larvae body. On determining effective washing of
-flupyrazofos, the washing volume to recover over 98% of
-flupyrazofos was observed at three times (each time: 1 mL). To select a suitable solvent, the recovery rates of each solvent in 3rd instar larvae of DBM were above 98%, but the washing rates of acetone, hexane and ethyl-acetate were 85.1%, 67.2% and 68.4%, respectively. In the BAW larvae, although the recovery rates of each solvent were above 99%, the washing rates of acetone, hexane and ethyl-acetate were 83.5%, 65.9% and 71.7%, respectively. The
-flupyrazofos were 0.731 h (44 min) in the DBM larva and 0.504 h (30 min) in the BAW larva. Radiocarbon in acetone washing (external fraction) decreased more quickly in the BAW larva than in the DBM larva, and amount of radiocarbon in larvae body increased more quickly with time in the DBM larva than in the BAW larva. In contrast, amount of radiocarbon in excreta increased more rapidly with time in the BAW larva than in the DBM larva.
Susceptibility of sweetpotato whitefly, Bemisia tabaci (Homoptera : Aleyrodidae) to commercially registered insecticides in Korea
Kim, Gil-Hah ; Lee, Young-Su ; Lee, In-Hwan ; Ahn, Ki-Su ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 51~58
These studies were carried out to investigate the toxicities of 43 registered insecticides to the sweetpotato whitefly(Bemisia tabaci, B. biotype). Insecticide activities were evaluated by testing systemic action and residual effect in the laboratory, and control efficacy in the greenhouse. All experiments were tested at the recommended concentration(ppm) of each insecticides. Insect growth regulators (IGRs), pyriproxyfen and teflubenzuron showed >95% ovicidal effect. The insecticides that showed >95% larvicidal activity on 3rd nymphal instars were abamectin, acetamiprid, imidacloprid, pyriproxyfen, and acetamiprid+ ethofenprox. Insecticides with >95% adulticidal activity were abamectin, acetamiprid, diazinon, endosulfan, fenitrothion, imidacloprid, methidathion, pirimiphos-methyl, pymetrozine, spinosad, acetamiprid+ ethofenprox, cartap kydrochloride+buprofezin, and fenpropathrin+fenitrothion. Abamectin, acetamiprid, imidacloprid, pyriproxyfen, and acetamiprid+ethofenprox showed both residual effect and systemic activity. In the control efficacy test on B. tabaci, 90% control values were obtained at 1st day after treatment of the insecticides including abamectin, acetamiprid, imidacloprid, pyriproxyfen and acetamiprid+ethofenprox but in pyriproxyfen, 90% control value was reached at 7th day after treatment. These results indicate that abamectin, acetamiprid, imidacloprid, pyriproxyfen and acetamiprid+ethofenprox can be used in control for B. tabaci in field.
Mating type and metalaxyl sensitivity of Phytophthora infestans isolated from Kangwon area in Korea
Kim, Byung-Sup ; Choi, Jin-Hoe ; Chun, Whan-Hong ; Ryu, Kyoung-Yul ; Hahm, Young-Il ; Lee, Youn-Su ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 59~63
Isolates of Phytophthora infestans obtained from several locations of Kangwon area in 1998 and 1999 were examined on mating types and sensitivity to metalaxyl. Both A1 and A2 mating type isolates were isolated in 1998 and 1999. The majority of the P. infestans isolates were A1 mating type. About 64.3% of the isolates collected in 1998 and 99.1% in 1999 were determined as A1 mating type. Sensitivity of the P. infestans to metalaxyl was examined by measuring mycelial growth on V8 juice agar amended with
matalaxyl. About 44.6% of the isolates examined in 1988 were resistant to metalaxyl, 55.4% of the isolates were intermediate resistant, but sensitive isolate was not isolated. However, 10.5% of the isolates examined in 1999 were sensitive, 88.6% of the isolates were intermediate resistant, and 0.9% of the isolates were resistant to metalaxyl. This studies indicate that A1 mating type is displacing A2 mating type and metalaxyl sensitivity of the P. infestans isolates of Kangwon area is increasing. This result is quite different from trends of early in 1990s.
Effect of phosphorous acid on control of phytophthora blight of red-pepper and tomato, and downy mildew of cucumber in the greenhouse
Chang, Tae-Hyun ; Lim, Tae-Heon ; Kim, Ik-Youl ; Choi, Gyung-Ja ; Kim, Jin-Cheol ; Kim, Heung-Tae ; Lee, Yong-Se ; Cho, Kwang-Yun ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 64~70
Control effects of phosphorous acid were investigated on three diseases. For Phytophthora blight of red pepper, protective and curative effects of phosphorous acid at the concentration of
a. i./mL were 91.0% and 80.0%, respectively. In case of late blight of tomato, caused by Phytophthora infestans, protective and curative effects were 63.4% and 13.0% at the same concentration, respectively. However, the protective and curative effects of phosphorous acid increased by decreasing inoculum density of Phytophthora infestans. The protective effects of phosphorous acid on control of Phytophthora blight of red pepper was persisted for 4 days with high control efficacy (94.0%). The protective and curative effects of phosphorous acid (
a. i./mL) on cucumber downy mildew were 82.0% and 62.0% respectively. The foliar application of phosphorous acid also promoted shoot growth and fresh weight of red pepper.
Effect of fruit quality, ethylene evolution and storage in apple 'Tsugaru' as influenced by aminoethoxy-vinylglycine and several surfactants
Kang, Chung-Kil ; Jung, Jae-Hoon ;
The Korean Journal of Pesticide Science, volume 4, issue 1, 2000, Pages 71~75
This experiment was conducted to evaluate the effect of fruit quality, ethylene evoluoon and storage in apple 'Tsugaru' as influenced by aminoethoxyvinylglycine(AVG) and several surfactants. When treated with AVG+Silwet L-77, there were few significant differences in soluble solid and acidity as compared with control, but dichlorprop treatment significantly increased in soluble solid and decreased in acidity. Color development was decreased when treated with AVG+Silwet L-77. AVG+Silwet L-77 treatment decreased in ethylene evolution, and increased in storage. It can be concluded that fruit can be left in trees longer and still maintain storage, and more fruit is able to go to fresh market into long-term storage, which increase the market-ability of apple.