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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
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Journal DOI :
The Korean Society of Pesticide Science
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Volume & Issues
Volume 5, Issue 4 - Dec 2001
Volume 5, Issue 3 - Sep 2001
Volume 5, Issue 2 - Jun 2001
Volume 5, Issue 1 - Mar 2001
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ELISA Development for the residue of the organophosphorus insecticide acephate
Lee, Jae-Koo ; Ahn, Ki-Chang ; Stoutamire, Donald W. ; Gee, Shirley J. ; Hammock, Bruce D. ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 1~12
A competitive indirect enzyme-linked immunosorbent assay (ci ELISA) for the organophosphorus insecticide acephate, O,S-dimethyl acetylphosphoramidothioate, was developed using a polyclonal antibody. Three different haptens mimicking the analyze and containing hexanoic acid moiety as a linker were synthesized, and then conjugated with the carrier proteins bovine serum albumin and keyhole limpet hemocyanin by the N-hydroxysuccinimide active ester method. Polyclonal antibodies raised against hapten-KLH conjugates in rabbits and the hapten-BSA conjugates as coating antigens were screened and selected for the assay in the homologous and/or heterologous ELISA system. The effects of various assay conditions, including blocking reagents, detergent content, organic solvents, pH, and preincubation of tile mixture of the polyclonal antibody and the analyze on the sensitivity were evaluated. The
value of acephate of 110 ng/mL was obtained in an optimized heterologous system using hapten-3-BSA as a coating antigen and a polyclonal antibody 8377, showing the detection range of 10-1000 ng/mL and the lowest detection limit of 4 ng/mL. The cross-reactivities of the structurally related insecticides, including methamidophos were less than 0.02%. These results indicate that the ELISA could be a convenient and alternative tool for monitoring acephate residues in agricultural products and environmental samples.
Synthesis of 2-phenylimino-1,3-thiazoline hydrochloride salts
Hahn, Hoh-Gyu ; Nam, Kee-Dal ; Shin, Sun-Ho ; Mah, He-Duck ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 13~17
A synthesis of new 2-phenylimino-1,3-thiazolines 2 for the purpose of development of new agrochemical fuugicide was described. Reaction of chlorine with diketene followed by treatment of benzylamine without isolation of intermediate 8a gave
benzylamide 10a. Thioureas 4 obtained from the reaction of methyl isothiocyanate with aniline derivatives were subjected to 10a in acetone solution to afford the corresponding 2-phenylimino-1,3-thiazoline hydrochloric acid salts 2 through unisolable inltermediates 11 and 12. The plausible reaction mechanism including nucleophilic attack of sulfur assisted neighboring nitrogen lone pair electron on phenyl of thiourea 4 was discussed.
Insecticidal Activities of Various Vegetable Extracts against Five Agricultural Insect Pests and Four Stored-Product Insect Pests
Lee, Sang-Gil ; Park, Ji-Doo ; Song, Cheol ; Cho, Kwang-Yun ; Lee, Sang-Guei ; Kim, Moo-Key ; Lee, Hoi-Seon ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 18~25
Ethanol extracts from 46 vegetables were tested their insecticidal activities toward five agricultural insect pests and four stored-product insect pests. The efficacy varied with both agricultural insects/stored-product insects and vegetable species used. Potent insecticidal activities, at the concentration of 5,000 ppm, were produced from extracts of Nelumbo nucifera and Ulva lactuca against Myzus persicae, Zea ways and Z. mays (leaf) against Nilaparvata lugens, Citrullus vulgaris (seed) and U. lactuca against Plutella xylostella, N. nucifera, Z. mays, and Z. mays (leaf) against Spodoptera litura, and C. vulgaris (seed), Daucus carota, Helianthus annuus (leaf), H. annuus (flower), Lactuca sativa, and Zingiber officinale against Tetranychus urticae. Potent insecticidal activities at the concentration of 2,500 ppm were exhibited from the extracts of N. nucifera and U. lactuca against M. persicae, Z. mays against N. lugens, C. vulgaris (seed) and U. lactuca against xylostella, N. nucifera and Z. mays against S. litura, and C. vulgaris (seed), H. annuus (flower), and L. santiva against T. urticae. Against four stored-product insect pests at 50 ppm, extracts of C. vulgaris (seed) and Cucurbita moschatla (seed) against Sitophilus oryzae and C. vulgaris (seed), H. annuus (seed), and Z. officinale against Plodia interpunctellfa revealed potent insecticidal activities over 80% mortality. In tests with Callosobruchus chininsis and Lasioderma serricorne, extracts of all vegetables tested exhibited meager and no activity.
Synthesis of trifluoromethylated dihydro-1,4-dithiin carboxamides and their antifungal activities
Hahn, Hoh-Gyu ; Nam, Kee-Dal ; Chang, Kee-Hyuk ; Lee, Seon-Woo ; Cho, Kwang-Yun ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 26~32
]-Unsaturated carboxamides 12 with trifluromethylated dihydro-1,4-dithiins were synthesized for the purpose of development of new agrochemical fungicide. Chlorination of trifluoromethylated
-ketoester 4 followed by tile reaction with 1,2-ethanedithiol gave intermediate 1,4-dithiane 9. Without purification of 9 substitution of hydroxy by chlorine followed by dehydrochlorination in the presence of triethylamine afforded trifluoromethylated dihydro-1,4-dithiin ethyl ester 7. Activation of the hydroxy of the carboxylic acid 10 obtained from the hydrolysis of 7 and then reacted with various amines gave the corresponding trifluoromethylated dihydro-1,4-dithiin carboxamides. Antifungal screening (in vivo) against typical plant diseases, Rice Blast, Rice Sheath Blight, Cucumber Gray Mold, Tomato Late Blight, Wheat Leaf Rust, and Barley Powdery Mildew of the synthesized compounds was carried out. As a result, most of the compounds shlowed weak antifungal activities and some compounds in which isopropyl group was substituted in meta of the phenyl showed antifungal activity (99%) at 250 ppm against the disease Wheat Leaf Rust.
Synthesis of new sulfonylureas and their herbicidal effects
Jeon, Dong-Ju ; Koo, Dong-Wan ; Ko, Young-Kwan ; Hong, Kyung-Sik ; Kim, Dae-Whang ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 33~36
New series of sulfonylureas containing substituted thiophene with 2-chloromethyl-1,3-dioxolane group were prepared, and their herbicidal effects were tested(in vivo) in the upland conditions and in the paddy submerged conditions. The sulfonylureas in which 4,6-dimethoxy- or 4-methyl-6-methoxy group in pyrimidine or triazine ring showed good herbicidal effects at a rate of 0.1 kg/ha. However, they showed weaker herbicidal effects than that of sulfonylureas containing substituted thiophene with 2-fluoromethyl-1,3-dioxolane group in general.
Protective Effect of Iminoctadine tris(albesilate) and Kresoxim-methyl Fungicides to Citrus Postharvest Diseases caused by Penicillium spp.
Hyun, Jae-Wook ; Lee, Seong-Chan ; Ihm, Yang-Bin ; Kim, Dong-Hwan ; Ko, Sang-Wook ; Kim, Kwang-Sik ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 37~44
The biological effects of the iminoctadine tris (albesilate) and kresoxim-methyl for the protection of citrus postharvest diseases caused by penicillium spp. were assayed. In vitro tests,
values of iminoctadine tris(albesilate) were
a.i./mL against mycelial growth of P. italicum and P. digitatum, respectively, but iminoctadine tris(albesilate) at
a.i. /mL inhibited a little mycelial growth of unknown Penicillium sp. which produced another symptom different to blue and green mold caused by P. italicum and P. digitatum, respectively. And against germination and growth of germ tube of P. italicum and P. digitatum,
value of iminoctadine tris(albesilate) was
a.i./mL. But spore germination of unknown Penicillium spp. was not nearly inhibited at
values of kresoxim-methyl were
, 0.04 and
a.i./mL against mycelial growth of P. italicum, P. digitatum and unknown Penicillium sp., respectively, and
a.i./mL against germination and growth of germ tube of P. italicum and unknown Penicillium sp., and P. digitatum, respectively. Iminoctadine tris(albesilate) and kresoxim-methyl were markedly effective to control the postharvest disease by 7 days spray prior to harvest. When the fruits were sprayed with iminoctadine-tris(albesilate) (
a.i./mL) and kresoxim-methyl (
a.i./mL) 7 days prior to harvest and subsequently stored for 90 days, the percentage of diseased fruit by Penicillium spp. was
in treatment of kresoxim-methyl and
in iminoctadine-tris(albesilate), respectively. On the other hand, tile percentage of diseased fruit was relatively high,
in thiophanate-methyl (
a.i./mL) and non-treatment, respectively. Maximum residue amount (ppm) among fruits (flesh and peel) assayed 0, 30, 60 and 90 days after storage was 0.45 and 0.10 ppm in treatment of kresoxim-methyl and iminoctadine, respectively.
Several causes of non virus-induced mosaic symptom on potato leaves and its induction by herbicides
Kwon, Min ; Hahm, Young-Il ; Kim, Hyun-Jun ; Yiem, Myoung-Soon ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 45~50
In recent, non virus-induced mosaic symptoms(NVMS) on potato leaves were observed in the seed potato fields, and its incidence rate was
nationwide. It made difficult to rogue out virus-infected plants, and caused much arguments between seed potato production farmers and seed potato inspectors. The objectives of these experiments were to find out the causes of NVMS, and also to induce mosaic symptom(phytotoxicity) on potato plants by treatment of several herbicides. No significant correlations were found between incidence rates of NVMS and values from soil analyses; soil pH, soil EC, organic matter content, and contents of inorganic constituents(
, Ca, Mg, K) in the soil around the potato planted. The examinations by ELISA, virus indicator plants, and TEM showed that NVMS on potato leaves was not caused by the viruses infection. But, the use of herbicides could induced the NVMS on potato leaves. The incidence rates of potato treated with pendimethalin linuron of 400 mL/10 a, pendimethalin of 200 mL/10 a, pendimethalin.oxadiazon of 300 mL/10 a, and control were 61.1%, 47.2%, 19.4%, and 1.4%, respectively. Based on these results, we confirmed that the treatment of pendimethalin alone and in mixture with other herbicides were the reason of NVMS on potato leaves. The yields among test plots were similar except dicamba treated plot, which decreased by about 23% compared to control plot. When their progenies harvested in 1999 were planted in the following season, no symptoms of mosaic were observed.
Controlling effect of several surfactants on barley powdery mildew caused by Erysiphae graminis
Jang, Kyung-Soo ; Yoo, Joo-Hyeon ; Choi, Gyung-Ja ; Kim, Jin-Cheol ; Cho, Kwang-Yun ; Kim, Heung-Tae ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 51~57
Controlling effect of several surfactants on barley powdery mildew (pathogen : Erysiphae graminis) and those influence on fungicidal activity by tank-mixing with commercialized fungicides were investigated in greenhouse test with barley seedlings. While protective activity of PNPP showed 90% at
, that of LN 13.0 91.2%, tile best among 8 surfactants used in curative test. With
, LN 13.0 showed 4% of disease percentage on the first leaf of narley continuously 4 days after the curative application. Excent for PNPP, the other surfactants, including NP 13.0, LN 13.0, and PAS, showed good inhibitory activity against spore germination in water agar medium amended with those surfactants. PAS, which showed very poor effect against barley powdery mildew in greenhouse test, strongly inhibited spore germination. Fungicide of triadimefon and triforine showed no controlling activity against Erysiphae graminis in narley seedlings, but they highly controlled powdery mildew in case of addition with
of LN 13.0 and Tween 20. SP 13.0, NP 13.0, LN 13.0 and DBC showed severe phytotoxicity in first leaf of barley seedlings, but Tween 20, SLIS, PAS, and PNPP not at
Chiral effect of fenoxaprop-ethyl on rice (Orysa sativa) and barnyardgrass (Echinochloa crus-galli)
Kim, Tae-Joon ; Kim, Jin-Seog ; Cho, Jeong-Sup ; Chang, Hae-Sung ; Cho, Kwang-Yun ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 58~61
A greenhouse study was conducted to evaluate the effect of R(+), S(-) and racemic mixture of fenoxaprop-ethyl on rice and barnyardgrass. In addition, in wire acetyl-coenzyme A carboxylase inhibition to those chiral compounds was determined. In the greenhouse trial, the R(+) and S(-) fenoxaprop showed respectively tile highest and the lowest biological activity on both plants. This dose-response in whole plant level was consistent with the result of in vitro dose-response of acetyl-coenzyme A carboxylase. These results corfirmed tllat the R(+) isomer is biologically more active than the S(-) isomer, and the target site of fenoxaprop is the enzyme acetyl-coenzyme A carboxylase. It was an interesting result that rice safety was improved in the S(-) isomer compared with the R(+), and the respective selectivity index was 1.5 and 0.57 in a greenhouse experiment; however, those values resulting from ACCase assay were not substantially different each other at in vitro level. Those results suggested that the fundamental selectivity of fenoxaprop-ethyl between rice and barnyardgrass would not exist at target site level.
Selection of insecticides for controlling Lycoriella mali in Letinula edodes sawdust cultivation
Kim, Gil-Hah ; Yoo, Jeong-Su ; Koo, Chang-Duck ; Lee, Sang-Gil ; Park, Ji-Doo ;
The Korean Journal of Pesticide Science, volume 5, issue 2, 2001, Pages 62~66
The thirteen commercial insecticides to Lycoriella mali were investigated on their insecticidal activities and mycelial growth of two Letinula edodes strains. For the adults, insecticides showing over 95% insecticidal activity were chlorpyrifos-methyl, fenthion, fenitrothion, benfuracarb, furathiocarb and deltamethrin. For tile larvae, diflubenzuron and cyromazine showed over 90% insecticidal activity. Fenthion, benfuracarb, furathiocarb, deltamethrin, diflubenzuron and cyromazine did not affect the mycelial growth of L. edodes strain, in Imhyup 1 variety. And deltamethrin, diflubenzuron and cyromazine did not affect that in Sanlim-5. Insecticides showing over 80% residual effect for 14 days were benfuracarb to the adults and diflubenzuron and cyromazine to the larvae. Control effect of furathiocarb, fenthion, benfuracarb, deltamethrin, diflubenzuron and cyromazine against larvae of Lycoriella mali showed 90% ten days after application under sawdust cultivation. Among them, benfuracarb, fenthion and furathiocarb revealed excellent control effect against adults. These results indicate that benfuracarb, fenthion, and furathiocarb can be used for the control of Lycoriella mali in the field. However, further studies are needed on the effect of insecticides treatment oil fruit-body yield and chemical residue in the mushroom tissues.