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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korean Journal of Pesticide Science
Journal Basic Information
Journal DOI :
The Korean Society of Pesticide Science
Editor in Chief :
Volume & Issues
Volume 7, Issue 4 - Dec 2003
Volume 7, Issue 3 - Sep 2003
Volume 7, Issue 2 - Jun 2003
Volume 7, Issue 1 - Mar 2003
Selecting the target year
Development of assay method for the activities of new compounds, and the effect of several fungicides against spore germination, adhesion, and myceial growth of Colletotrichum sp. causing red pepper anthracnose
Kim, Jae-Jeung ; Kim, Joon-Tae ; Park, Sung-Woo ; Park, Eun-Suk ; Kim, Heung-Tae ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 159~168
With microtiter plate, the assay method was developed for detecting the fungicidal activity of new compounds against spore germination, spore adhesion and mycelial growth of Colletotrichum sp. JC24 cal1Sing red pepper anthracnose. Also, the effects of some commercialized fungicides on fungal development like above mentioned were investigated by measuring the optical density of mycelia grown into wells of microtiter plate. For the standardization of assay method, some factors, such as the treatment of MTT and/or propanol, inodulum density and incubation period, affecting on mycelial optical density were investigated. For obtaining precise and consistent mycelial optical density, it was necessary the treatment of MTT for 12 hrs and propanol for 1 hr. inoculum density adjusted to
spores/mL and incubation period for 36 hrs at
. For fungicidal activities, 6 protective fungicides, 6 ones inhibiting sterol biosynthesis, and one inhibiting respiration were used in this study. While mancozeb, chlorothalonil and dithianon among 6 protective fungicides inhibited strongly spore germination, adhesion, and mycelial growth at
, propineb, iminoctadine and fluazinam inhibited intermediately spore germination and mycelial growth at
. Washing above 3 fungicides with new PD broth, their activity against spore adhesion decreased. With hexaconazole, tebuconazole and myclobutanil, the tendency of the activity against fungal differentiation of the early infection stage was similar to the latter group of protective fungicides, showing the decrease of the inhibitory activity against spore adhesion by washing 2 hrs after incubation. However, kresoxim-methyl inhibited spore adhesion distinctly, depending on the applied concentrations. Based on these results, it might be able to assess the fungicidal activity of many compounds against spore germination, adhesion and mycelial growth by the use of microtiter plate in vitro. Using the assay developed in this report, it was possible to investigate the inhibitory activity of some commercialized fungicides, too.
Safe and easy disposal of prochloraz wastewaters after used as rice seed disinfectant
Park, Byung-Jun ; Choi, Ju-Hyeon ; Kim, Chan-Sub ; Lee, Byung-Moo ; Ihm, Yang-Bin ; Cho, Il-Kyu ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 169~175
For safe and easy disposal of prochloraz wastewaters after used as rice seed disinfectant in Korean farms, this experiment was carried out. By addition of several agricultural materials commonly utilizing in farmers, removal effect of prochloraz from waste solution was also investigated. When rice seeds after soaking in diluted prochloraz solution were rinsed with water several times, prochloraz was removed
at the first rinse and less than 3 % at the fourth rinse. A half life of prochloraz was
days in aqueous system. Hydrolysis of prochloraz was more rapidly in alkali solution than neutral and acidic one at
. By the irradiation under
using xenone lamp, prochloraz was photo-degraded to 87.7% in aqueous system. The removal efficiency of prochloraz by addition of several agricultural materials were as follows: 93.6% by lime, 90.7% by composed pig manure, 89.4% by activated charcoal, 78.0% by straw ash, 70.3% by sandy loam soil, 47.0% by zeolite and 24.1 % by rice straw. When prochloraz solution was sprayed on the field soil, it was dissipated upto 90% within 35 days.
Leaching behaviour of the herbicide mefenacet in the soil columns
Kim, Sung-Min ; Kwon, Jeong-Wook ; Ahn, Ki-Chang ; Cho, Il-Kyu ; Kyung, Kee-Sung ; Lee, Jae-Koo ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 176~181
The leaching behaviour of [aniline-
]mefenacet in soil was investigated using glass columns (5 cm I.D.
30 cm. H) packed with two types of soils with different physicochemical properties.
-Mefenacet (8.33 kBq) and mefenacet (in total, 1.05 mg/kg) were treated onto soil columns and rice plants (Oryza sativa L.) were grown for 17 weeks on these columns. Leachates from the columns were collected at the rate of 122.5 mL per week.
-Activities leached from soil A (OM, 3.1%; CEC, 86 mmol(+)/kg; texture, loam) columns with and without rice plants were 1.95 and 4.19% of the originally applied, whereas those from soil B (OM, 1.3%; CEC, 71 mmol(+)/kg; texture, loam) were 2.69 and 7.05%, respectively. These results indicated that larger amounts of
were percolated from soil B with less organic matter and from the columns without vegetation.
-Activities absorbed by rice plants from soil A and B were 8.95 and 8.47%, respectively, most of which remained in the root and shoot excluding unhulled grains and ears without grains.
of the originally applied
remained in the depth of
in soil. The mass balance indicated that the losses by volatilization and/or mineralization amounted to
of the originally applied.
-Radioactivities in the aqueous phase of the leachates ranged from 59.4 to 97.7% of the radioactivities in leachates, showing the fast transformation of mefenacet to the polar metabolites.
Influence of soil organic matter and moisture on the persistence of the herbicide mefenacet in soils
Kim, Sung-Min ; Cho, Il-Kyu ; Kyung, Kee-Sung ; Lee, Jae-Koo ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 182~187
In order to elucidate a degradation characteristics of herbicide mefenacet in soil, the persistence in soils was studied under laboratory conditions for
. Mefenacet residues were determined from the two soils which pre-treated by sterilization and flooding, respectively. Non-sterilized upland soil was used as a control. When 70 days elapsed from application time,
of mefenacet applied were dissipated in control soils. However,
of mefenacet applied were dissipated in the sterilized soils and
was dissipated in the flooded soils. 까 lese results indicated that the degradation of mefenacet was assumed to be due to microorganism, especially aerobic microbes. In order to elucidate the influence of water content on the persistence of mefenacet in soil, water content in soils was adjusted to 20, 50, and 80% of the water-holding capacity(Field capacity, WHC). The half-life of mefenacet in soil containing 20% and 50% of WHC were 82 and 73 days, respectively, after incubation for 90 days. However, the half-life in soil containing 80% of WHC was shortened to 61 days. These results indicated that degradation of mefenacet in soil was influenced by the activity of soil microorganism, organic matter content and water content.
Effects on EDC-like farming chemicals in aquatic Organism
Kim, Hyun-Woo ; Park, Kun-Ho ; Park, Jin-Hong ; Jin, Hua ; Kim, Joon-Seong ; Eu, Gook-Jong ; Cho, Hyun-Sun ; Kang, Ga-Mi ; Lee, Myung-Sung ; Song, Byung-Hoon ; Shin, Jin-Sup ; Cho, Maing-Haing ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 188~197
Endocrine disrupting chemicals (EDCs) can alter hormone regulation that control reproductive system in animals. The effects of endosulfan, molinate, and alachlor that suspected to have examined disruption EDCs effect on a fish species of interest, Xiphophorus helleri (swordtail fish), were studied using vitellogenin (Vtg) and aromatase as diagnostic biomarkers. Induction of Vtg proteins was detected by RT-PCR in male fish treated with alachlor, and mixture of endosulfan and molinate in dose response manner. Also, induction of aromatase was detected by RT-PCR in male fish treated with alachlor, endosulfan, and mixture of endosulfan and molinate in sinlilar manner. In this study, swordtail fish exposed to endosulfan or molinate individually did not show any adverse effects. However, Vtg and aromatase expressions and apoptosis were detected in swordtail. fish exposed to the mixture of endosulfan and molinate. These results suggested that low concentrations of mixture of molinate and endosulfan individually do not affect swordtail fish, but may influence genital system, and induce apoptosis.
Risk assessment on cytotoxicity for benzimidazole fungicides
Lee, Je-Bong ; Sung, Pil-Nam ; Jeong, Mi-Hye ; Shin, Jin-Sup ; Kang, Kyu-Young ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 198~206
To assess potential risk of the benzimidazole fungicides, their cytotoxicities were evaluated. Activities of LDH(Lactic dehydrogenase) in the culture fluid of CHL(chinese hamster lung) fiberoblast cell treated with 4.0, 16.0 or
of carbendazim for 24 hours were elevated 2.16, 2.94 and 2.64 folds compared to the control, respectively. DNA synthesis was inhibited by 45% at
of carbendazim. Benzimidazole fungicides showed high toxicity to cell and mitochondria of CHL cell by Giemsa and MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay.
by the Giemsa assay of thiophanate-methyl, benomyl, carbendazim and captafol were over 125, 1.2, 30.0 and
by the MTT assay of thiophanate-methyl, benomyl, carbendazim and captafol were over 125, 18.7, 20.4 and
, respectively. Inhibitory concentration of cell median proliferation by SRB (sulforhodamin B) assay for thiophanate-methyl, carbendazim, benomyl, and captafol were 17.4, 5.3, 1.5 and
, respectively. Accordingly, benzimidazole fungicides inhibited DNA synthesis, mitochondrial function, cell proliferation and induced cell necrosis.
Toxicities and Control Effect of Three Insecticides to Greenhouse Whitefly, Trialeurodes vaporariorum and Sweetpotato Whitefly Bemisia tabaci (Homoptera: Aleyrodidae)
Ha, Tae-Ki ; Hwang, In-Cheon ; Kim, Jong-Kwan ; Song, Yoo-Han ; Kim, Gil-Hah ; Yu, Yong-Man ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 207~215
This study was carried out to evaluate toxicities of 3 registered insecticides to greenhouse whitefly(GWF), Trialeurodes vaporariorum and sweetpotato whitefly(SWF), Bemisia tabaci, B-biotype. Insecticide activities were evaluated by testing systemic action, residual effect in the laboratory, and control efficacy in the greenhouse. All experiments were tested at the recommended concentration(RC), half and a quarter concentrations of RC of each insecticides. Acetamiprid showed 45%, 42% ovicidal effect to greenhouse whitefly and sweetpotato whitefly at 40 ppm, respectively. Acetamiprid showed more than 97% larvicidal activities on the 3rd instars larvae of GWF and SWF at the recommended and its half concentrations. On the adults of the two whitefly species, acetamiprid and acetamiprid+ethofenprox showed more than 92% mortality even at half of recommended concentrations. Acetamiprid and acetamiprid+ethofenprox showed both residual effect and systemic activity. In the control efficacy test on GWF and SWF, 90% control values were obtained at the 3th day after treatments of acetamiprid and acetamiprid + ethofenprox by application with recommended concentration. These results indicate that acetamiprid and acetamiprid+ethofenprox can be used in the control of the two whitefly species in field.
Influence of water temperature, soaking period, and chemical dosage on Bakanae disease of rice (Gibberella fujikuroi) in seed disinfection
Park, Heung-Gyu ; Shin, Hae-Ryong ; Lee, Yeen ; Kim, Suk-Wean ; Kwon, Oh-Do ; Park, In-Jin ; Kuk, Yong-In ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 216~222
In order to develop effective control methods for Bakanae disease caused by Gibberella fujikuroi (Saito) Ito during rearing of rice seedlings, we investigated the disease resistance of 15 rice varieties to G. fujikuroi and control effect of six seed disinfectants, and tried to improve the using methods of the seed disinfectants. Disease resistance was tested by investigating the disease incidence on each rice cultivar grown in rice seedling box infested with or not infested with G. fujikuroi at 30 days after sowing seeds and 20 days after heading date. The results showed that Hwayongbyeo, Dongjinbyeo, Hwoanbyeo, Nonghobyeo, Nampyeongbyeo, and Hwojinbyeo were resistant G. fujikuroi, meanwhile Keulubyeo, Sobibyeo, Odaebyeo, Junambyeo, Samchonebyeo, Sangjubyeo, and Hwabongbyeo were susceptible. Three seed disinfectants, prochloraz, fludioxonil, and carproamid + thiram + fludioxonil controlled Bakanae disease of rice very well, while bonomyl + thiram, thiophanate-methyl + thiram and thiophanate-methyl + triflumizole did not suppress the disease enough. Water temperature was turned to be an important factor for controlling the disease by treating seed disinfectants. Prochloraz showed 61% control value on the disease at
, but it showed above 95% control value at the range of
. It was confirmed that the control effect of seed disinfectants increased with increasing water temperature. Meanwhile soaking period of rice seeds in the suspension of seed disinfectants and chemical dosage had no high relation to control the disease. This results suggest that rice varieties, water temperature, and optimal selection of suitable seed disinfectants are very important to control Bakanae disease effectively.
Control of downey mildew occurred on cucumber cultivated under plastic film house condition by optimal application of chemical and installation of ventilation fan
Kim, Yong-Ki ; Ryu, Jae-Dang ; Ryu, Jae-Gee ; Lee, Sang-Yeob ; Shim, Hong-Sik ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 223~227
Survey on plant diseases occurring on cucumber cultivated in plastic film house of experimental farm in Suwon was conducted. Through the survey, occurrence of damping-off, downey mildew, powdery mildew and Fusarium wilt was observed. Especially downey mildew caused by Pseudoperonospora cubensis was the most severe foliar disease of cucumber. To control the disease effectively, effects of installation of ventilation fan and optimal spray timing of a chemical, dimethomorph+copper oxychloride WP, were investigated. Two ventilation fans installed at the front and at the back of plastic film house reduced air relative humidity by about 6.4% and downey mildew incidence by 55.7%. Downey mildew incidence on cucumber from untreated chemicals plot in plastic film house installed with ventilation fan was on a equal level with that from treated chemicals plot with three times application of dimethomorph+copper oxychloride WP in plastic film house without ventilation fan. Meanwhile in order to select optimal chemical application time, dimethomorth+copper oxychloride WP was treated three times at 7 days-interval from three days before the disease occurred, right after the disease occurred, and two days after the disease occurred, respectively. The result showed that dimethomorth+copper oxychloride WP applied to cucumber leaves and stems from three days prior to, right after, two days after occurrence of downey mildew reduced downey mildew incidence by 72.9, 61.8, and 23.7%, respectively. The above results showed that regulation of environmental factors like air relative humidity and preventive application of chemicals should be considered to establish control strategy to downey mildew.
An aspect of quarantine insect pest occurrence with different management system in sweet persimmon orchard
Lee, Dong-Woon ; Park, Jae-Wan ; Park, Chung-Gyoo ; Choo, Ho-Yul ; Kim, Young-Sub ;
The Korean Journal of Pesticide Science, volume 7, issue 3, 2003, Pages 228~237
Temporary control schedules were tested at sweet persimmon orchards to development new control programs to meet the quarantine repuirements of America in 2001 and 2002. The 'MRL-type control orchards' were sprayed with chemicals which were possibly adaptable to the pome trees in America. A control schedule consisted of those chemicals registered for persimmon in Korea was incorporated in the 'domestic-type control orchards'. The efficacy of these two control type against insect pests was compared with that of a conventional control schedule. In 2001, MRL orchard and domestic orchard were sprayed 7 and 6 times, and two conventional orchards were 6 and 9 times, respectively. In 2002, acaricide was added once to the MRL orchards at late September to reduce the density of mites on harvested fruits. However no insecticide to plant bug control could be applied to the MRL orchards, because no insecticide against bugs was registered for pome trees in America. This resulted in 7 times of applications in MRL and domestic orchards. The conventional orchard was sprayed 9 times. Only the occurrence of the peach pyralid moth, Dichocrocis puntiferalis (PPM) out of 4 quarantine inset species was observed. The PPM was observed during growing season in MRL, domestic, and conventional orchards. However no fruits damaged by PPM larvae were observed after mid October and after harvest. In 2002 only 1 fruit out of 1,350 fruits inspected in June was damaged by the larvae of PPM at MRL orchards. A fungus-feeding mites and collembolan were under calyx of vested fruits. In 2001 they were found on 45.3% of harvested fruits at MRL orchard. However the percentage of fruits with mites in 2002 was greatly reduced to 3.5% at MRL orchard, presumably because of a added application of acaricide at late September. However percentage of fruits damage by hemipteran bugs at harvesting time was quite high 11.3 % at MRL orchards, because no application of insecticide against plant bugs.