Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean journal of applied entomology
Journal Basic Information
Journal DOI :
Korean Society of Applied Entomology
Editor in Chief :
Volume & Issues
Volume 32, Issue 4 - Dec 1993
Volume 32, Issue 3 - Sep 1993
Volume 32, Issue 2 - Jun 1993
Volume 32, Issue 1 - Mar 1993
Selecting the target year
Insecticide Susceptibility of Field-Collected Populations of the Spiraea Aphid, Aphis citricola(van der Goot)(Hemiptera: Aphididae) in Apple Orchards
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 259~264
The experiment was carried out to investigate Insecticide susceptibility of the spiraea aphid (Aphis citricola) in apple orchards In Korea, using dipping method. Although insecticIde susceptibility vaned with local and seasonal populations, the susceptibility to demeLon S-rnethyl and phosphamidons was different from that Lo the other insecticides. The LCso values were 10 ppm for deltamethrin and chloropyrifos, 103 ~ 629 ppm for demeton S-methy1, acephate, phosphamidon, monocrotophos and vamldothion, 12,200 ppm for EPN, and 1,745 ppm for pirimicarb. 'When Insecticide susceptibility was compared between the S-clone selected from a population on the host plant spiraea and the R-clone selected from a population on the apple tree, the resistance ratio as expressed by RIS ratio of
was 78 for phosphamidon and 546 for pirimicarb. Esterase zymogram determined by the agar gel electrophoresIs revealed a significant difference between the clones. The activity of the E2, E5, E6 and E7 of the R-clone was higher than that of the S-clone, It is suggesLed that the Increased esterase activity may be involved in the mechanism of insecticide resistance in the spiraea aphid, although the involvement of other factor(s) may not be ruled out.
Studies on the Insecticide Resistance of the German Cockroach(Blattella germanica L.). III. Comparison of Esterase Activity
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 265~270
The German cockroach(Blattelia germanica) population~ were successIVely selected with ch\orpyrifos and permethrin during the six generations. The resulting resistant
(Rp) stra.ins were studied to investigate the esterase activity by spectrophotometer, filter parper test, and electrophoresis. Esterase-
activities by filter paper test showed 2.65 and LBZ times higher in the Rc and Rp strains than the susceptible strain, respectively. ln the spectrophoLometer method, the esterase activit18s to
-naphthyl acetate were increased 2.34 and 5.28 times in the Rc than susceptible strain, and 1.48 and 2.92 times in the Rp Limn susceptible stram, respectlvely. Zymogram patterns of eslerase isozyme by agarose gel electrophoresis showed totally five bands. The Rc and Rp strains showed two additive bands as, Est-2 and Est-3, which were not shown in the susceptible strain. but the Rp strain dId not show Est-5 bands which was COlumon in the Rc and susceptible strams.
Influence of Honeybees Pollinationon Soybean Yield and Yield Components
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 271~278
This experiment was carried out to elucidate the mfluence of honeybees pollinatIOn on yield and YIeld components of soybean at the Kyungbuk Provincial Rural Development Administration from 1990 to 1991. SIX soybean varieties were cultivated in net houses with and without bees. Italian honeybees(Apis melhfem L.) were used. The Vlsiting frequency of bees in net house wIth bees was 58% higher than that of field honeybees, and it was the highest at 10 A.M. during a day. Number of pods per plant and podding ratio of soybean were higher with bees compared with Wlthout bees, especially for the varieties Paldalkong and Dankyongkong. The number of seeds per pod was not significantly different between with bees and without bees, although the percentage of three seeded pod of varieties Danyeobkong and Muhankong and one seeded pod of varieties Hwangkeumkong and Milyangkong 'was increased. The percentage of three seeded pod of Paldalkong and Dankyeongkong, however, was decreased with bees compared with without bees. At the same time no varietal difference was found in two seeded pods between with bees and without bees. Yield of soybeans was increased 0 to 12 % with bees compared with that of without bees. Yield increase was the hIghest in Paldalkong and Dankyongkong. The ratIO of soybean hybrid plant was mcreased with-bees compared with that of without-bees, especially m Paldalkong and Dankyongkong. A negative correlation was found between the ratio of hybrid plant and the duration of flowering in soy¬beans.
Ecological Characteristics of Adoxophyes sp. at Tea Tree Plantation
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 279~284
This experiment was conducted to investigate the seasonal fluctuation of population density and the life history of Adoxophyes sp .. Under field-cage conditions, smaller tea tortrix had 4-5 generations a year. The average periods from egg to adult emergence were 43.7 days in spring and fall, and 35.2 days in summer. The average longevity of adults was 7.0 days in spring and fall, and 6.3 days in summer. The average numbers of egg masses and eggs laid by a female were 1.8 and 40.4 m spring and fall, and 2.3 and 49.5 in summer, respectively. Population density of smaller tea tortrix estimated by sex pheromone trap and light trap showed four peaks in each late May, late June, middle August and late September. The number of individuals caught by sex pheromone trap was 2~4 times as large as that by jight trap. Smaller tea tartrix overwimered in larval stage in southern region af Korea.
Natural Enemies of Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae) and Their Seasonal Prevalence of Adult Emergence in Korea
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 285~290
This study was conducted to mvestigate the natural enemies of chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu and their seasonal prevalence of adult emergence. The natural enemies Identified were as follows: Torymus sinensis, Torymus geranii, Eutytoma Brunniventris, Eurytoma s Setlgera, Megastigmus nipponicus, Megastigmus maculipennis, Ormyrus flavrtrbwlis, Ormyrus punctiger, Eupelmus urozonus, Euplmus sp., Sycophila vanegata, and Diptem sp. Among these natural enemies of the chestnut gall wasp, Torymus sinensis, Eurytoma setigem, and Eupelmus sp. were newly recorded this time In Korea. The seasonal prevalence of parasItic wasps have been investigated in Kyonggi ProvInce. Torymus sinensis which seems to be the most dominant parasitoid emerged from withered galls formed in the previous year April to eary May. The four parasitoids such as Torymus geranii, Sycophila variegata, Eupelmus urozonus and Eupelmus sp. emerged from withered previous year galls in April to May and also from current year galls in June to August. The other parasitoids emerged from only current year galls in June to August.
Activity of Mixed Function Oxidase in a few Insect Species in Relation to Their Food Source
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 291~299
Midgut tissues from 4 insect specIes were exammed for the activity of cytochrome P-450 monooxygenases, a major enzyme involved in chemical detoxification. When Helicoverpa assulta larvae were reared on an artificial d;et, the specific activity of the midgut cytochrome P-450 monooxygenases (MFO) was :3 times higher than that of the fat body, The specific activity of the midgut cytochrome P-450 monooxygenases was higher in H. assul/a larvae when reared on Nicotiana tabacum leaves than when on CapsIcum annuum fruits or an artificial diet. In the case of Hyphantria cunea larvae, Tilia megaphyllo leaves were the best in inducing midgut cytochrome P-450 monooxygenases activity. When larvae of H. assulta, Spodoptera exigua, H. cunea and Spodoptera litura were reared on their own artificial diet, the highest activity was seen in S. exigua larvae which is a polyphagous and insecticide-resistant strain.
Cloning and Expression of an Insecticidal Crystal Protein CryIIA Gene from Bacillus thuringiensis subsp. kurstaki HD-1
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 300~306
The CryIIA gene encoding the insecticidal crystal protein of Bacillus thuringiens!s subsp. kurstalri HD-l has been cloned in Escherichia col!, and its nucleotide sequences were determined completely. 5kb Hindlli fragment harboring CryIIA gene was screened in the large ca. 225kb plasmid DNA by southern blot. HindlIT digested 5kb fragment was ligated into pUC19 and transformed in E. coli. The 4kb BamHI-HindlIT fragment containing the CryIIA gene was subcloned and named pSKIIA. DNA sequence analysis demonstrates that pSKIIA is the gene of an operon which is comprised of Lhree open reading frames (designated orn, orf2 and or£3). The CrylIA gene is composed of 3,952bp-long BamHI-Hindill DNA restriction fragment. The orf3 code for a polypeptide of 633 amino acid residues. The protoxin protein has a predicted molecular weight of 70,780. The E. coli derived protoxin gene product is biologICally active against three species of Lepidopteran (Plu.lelia maculipennis, He/iolhis assulta, Spodoptera litura) and a species of Dip Leran( Culex pipines) larvae in bioassay.
Use of Geographical Information Systems in Analyzing Large Area Distribution and Dispersal of Rice Insects in South Korea
;K. L. Heong;
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 307~316
The potential of using GIS in analyzing pest surveillance data was explored. The Spatial Analysis System (SPANS) was used to construct a spatial data base to study pest distributions using pest surveillance data collected from 152 stations in South Korea. The annual spatIal distributions of the striped rice borer(SRB), Chdo suppressalis, showed that high densities started to expand in the early 1980s, reaching a peak in 1988. The pattern change appears to be related to cultivation of japonica and indica-japonica hybrid varieties in South Korea. Japo7l!ca varieties have longer duration resulting in the SRB haVlng more time to mature and hibernate in wmter. The locus of SRB spread appears to be located in the mid-west region near lri, Chun~ Buk Province. High brown planthopper (BPH) populations in South Korea are often related to the early immigration and temperature. The simulated distribution of PPH densities in September using these two factors was compared with the actual distribution obtained using 1990 data. The two density maps corresponded closely excepL for differences in the south eastern valley. By overlaying the simulated map layer with the elevation and rice area maps, more specific BPH risk zones could be identified.
Classification of the Three Korean Biotypes of the Brown Planthopper, Nilaparvata lugens(Stal), By Morphological Variation
;;R. C. Saxena;A. A. Barrion;;
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 317~322
Morphological investigations of the abdominal lateral lobes in females and the unguitractor p plates in the tarsi of legs in male and female N. lugens among the three Korean N. lugeηs bio-types revealed that the three biotypes varied from one another. The presence of a distinct ‘cut’ on the lateral lobes in brachypterous females distinguished Biotype 2 from Biotypes 1 and 3. The highest frequency of inserted unguitractor plates in fore-and mid-tarsal segments was exhibited by Biotype 2 in both sexes of each morphs.
Comparative Study of Toxicological Methods and Field Resistance to Insecticides in Diamondback moth(Lepidoptera: Plutellidae)
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 323~329
These studies were conducted to evaluate the five comparative test methods for detecting chemical resistance and to investigate resistant level of field populations of diamondback moth (Plutella xylostella L.). Leaf disc method was practically rocomrnendable because of its rapidity and low CV(l1.4%). Topical application method was a precise replicabiliLy(CV=8.00/0) but it was time consuming and difficult in mampulation. The other 3 methods showed higher CV ranging from 14.9% to 21.4%. Based on
values by topical application method, field populations of diamondback moth collected from 4 different regions, Kwangju, Kimhae, Jeju, and Inje to prothiofos showed from 3.3 to 61.1 times higher resistance than the susceptible strain, whereas to cypermethrin, Lhey were from 7.5 to 141.7 times higher than the susceptible. To cartap hydrochloride, they showed from 10.5- to 33.3-fold resistant levels as high as the susceptible. Finally, based on
values to Bacillus thuringiensis by leaf disc technique, the resistant levels of the field populations were from 1.9 Lo 8.1 times as compared to the susceptible.
Molecular Approaches to Determine the Character of Serratia marcescens Associated with the Insect Pathogenicity to Brown Planthopper
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 330~337
A bacterium, pathogenic to Nilaparvata lugens Stal. causing high mortality in 3~5 days, were selected and identified as Serratuz marcescens biotype A2a which is not a nosocomlally infective strain. In order to determine the characters of Serratia marcesce'1lS associated with insect pathogenicity, Tn5 mutagenesis was carried out by conjugating with E. coli pJB4J1. Transconjugants were plate-assayed for missing chitinase, protease and DNase activity. A protease negative mutant was selected for missing JOseet pathogenicity. SEM and TEM revealed the presence of bacterial cells in the epithelial tissue of inner abdomal tissue of the hypodermic layer of abdomen. Such a colonization was limmited to the subjacent tissue inside the intacL cuticular epidermis. These observation supported our result of pathogenicity tests of transconjugants.
Host range and Bionomics of the Rhombic Marked Leafhopper, Hishimonus sellatus Uhler(Homoptera: Cicadelliae) as a Vector of the Jujube Witches-Broom Mycoplasma
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 338~347
The study was to investigate the bionomics, host plants, and oviposition preference of Hishimonus sellatus Uhler. It has 5 generations under natural conditions and the peak of the 3rd generation was observed about mid and late August. Its average developmental durations were 80 days in spring, 69 days in summer, and 77.8 days in autumn. The lengths of each stage were 0.8mm in eggs, 0.9mm in 1st instar, 1.4mm in 2nd instar, 2.1mm in 3rd instar, 2.5mm in 4th instar, 3.2mm in 5th instar, 4.1mm in female, and 3.8mm in male. Hishimonus sellatus overwintered as egg in Morus alba, Humulus japonicus, and Zizyphus jujuba begining mid October, and attacked the shoot of M. alba and H. japonicus about mid and late May, migrated to the Zizyphus jujuba from late June to early July. Female oviposites 32~62 eggs into epidermis of shoot, vagina and vein during their life. The preferred host plants of H. sellatus were Humulus japonicus, Morus alba, Zizyphus jujuba, and ligustrum obtusifolium. Highly preferable oviposition site was H. japonicus, M. albal, Z. jujuba, and L. obtusifolium, etc. On audlt longevity, the host plants as H. japonicus, M. alba, and Z. jujuba were 43
2 days and A. brevipedenculata, C. mimosoides, L. obtusifolium, V. rosa, A. sinicus and, A. graveolens were more than 25 days, and other host plants were less than 20 days.
Seasonal fluctuation of Carboxylesterase activity in field collected populations of the green peach aphid
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 348~353
The fluctuation of insecticide resistance in the green peach aphid (GPA) in fields was investigated by caboxy1 esterase (CE) activity index analysis. Of the GP A Populations occurred on the red pepper seedlings, aphids in the untreaLed and treaLed with acephate plots showed 40 and 78 resistance percent (RP), respectively. Aphids in the untreated kale field showed the RP value 24 in July, contrast to 83 in October. Mean RPs of aphids from 18 localities were 50 + 14 in summer and B2+ 10 in late fall, indicating a seasonal fluctuation of Lhe CE activity
Quantitative Observation on the Behavior of the Smoky Brown Cockroach, Periplaneta fuliginosa(Seville): Presence at Important Micro-havitats of Rearing Cages in the Laboratory
Korean journal of applied entomology, volume 32, issue 3, 1993, Pages 354~371
Behavior of adult females of the smoky brown cockroach was observed for 10-15 days continuously by using computer and the automatic sensoring system. Under the light condition of l2L~ 12D, individual variations were generally higher and the periodicity appeared less in terms of the presence time at the micro-habitats and the locomotory activity. The smoky brown cockroach appeared 20.42, 11.50, 6.31 and 2.66 % in a day in averages respectively at the shelter and the places for other individuals, feeding and drinking. It stayed 20.29 % in a day at the shelter when food, water, and other individuals were not supplied. Visiting rates were higher at the feeding and dnnking places than at. the ot.her micro-habitats. The degree of t.he locomotory activity was relatively lower when food, water, and other individuals were not supplied t.han when they were supplied. The Pnncipal Component Analysis (PCA) on the presence at the micro-habitats showed that. t.he presence pattern for each mdividual appeared differently (Q mode) while the differem time zones were grouped to the photophase and scotophase (R mode). When food, water, and other individuals were supplied the degree of grouping was higher at the shelter than at the places for feeding and drinking. When the act.ivityand the presence time at the different micro-habitats were jointly analyzed by PCA, the achvity of the female smoky brown cockroach appeared in relation with the presence time ( %) at the places for feeding and other individuals.