Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean journal of applied entomology
Journal Basic Information
Journal DOI :
Korean Society of Applied Entomology
Editor in Chief :
Volume & Issues
Volume 36, Issue 4 - Dec 1997
Volume 36, Issue 3 - Sep 1997
Volume 36, Issue 2 - Jun 1997
Volume 36, Issue 1 - Mar 1997
Selecting the target year
A New Species of the Genus Scaeosopha Meyrick (Leipidoptera: Cosmopterigidae, Scaeosophinae) from Korea
Ponomarenko, M. G. ;
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 287~289
A new species, Scaeosopha sinevi sp. nov., is described from Korea. This genus is reported for the first time from the Palaearctic Region. The genus Scaeothyris Diakonoff, 1968 is newly synonymized with Scaeosopha Meyrick, 1914.
Eight Newly Recorded Species of Depressarriidae(Lepidoptera) from Korea, with Five New Synonyms
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 290~298
Eight species of Depressariidae,
, are reported for the first time from Korea. Depressria niphospyrphos Meyrick and D. basicosta Matsumura are newly synonymized with D. taciturna Meyrick. Agonopterix sapporensis (Matsumura) and A. ochroephala Saito are also synonymized with A. angeliocella(Hubner) respectively, and A. iharai Fujisawa with A. ocellana (Fabricius).
Temporal Pattern of Within-harborage Time and Visiting Frequency in Two Strains of the german Cockroach, Blatella germanica, in Semi-natural Conditions
;;Mary H. Ross;
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 299~310
A mutant, or(orange body), and the KNIH(Korea National Institute of Health) strain of the German cockroach, Blattella germanica(L.) (Orthoptera: Blattellidae), were observed continuously in semi-natural conditions through video taping and data processing by computers. Male adults were individually observed for 4-5 days in a rectangular-shaped rearing cage with four different microhabitats, allowing observations of behavior such as resting, feeding, drinking and communicating with other individuals. The frequencies of visits to and times spent within harborage were determined. Although there were relatively large variations among individuals, the average stays withing harborage ranged from 47-61% of the total observation time. The duration of within-harborage time was significantly different between maternal origins, while not distinctively different between the strains. Diel differences were observed in body strains. The time spent within harborage was longer in photophase than in scotophase. visiting frequency, which represented local activity around harborage, appeared slightly higher in the or strain than in the KNIH strain, and was significant in the or strain while it was not significant in the KNIH strain. Although there were variations among individuals, similar temporal patterns in some progenies from the same maternal origins were observed in time spent within harborage and in visiting frequency.
Effects of Temperature on the Development of Green Mirid Bug, Cyrtorhinus lividipennis Reuter(Hemiptera: Miridae) and Predation of Planthoppers Eggs by Its Adult
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 311~316
This study was conducted to evaluate the effect of temperatures on the egg and nymphal development andadult longevity of green mirid bug, Cvrtorhinus lividipennis Reuter. In addition, predation on eggs of brownplanthopper, Nilupurvurcz lugens ~t:l and small brown planthopper, L~orlelphux .striatellus Fallen by C.lividipennis were studied at different temperatures. Hatchability of C. lividipennis was about 96% at 23"C,88% at 26"C, 75% at 29
C and 64% at 32
C. Egg duration of C. lividipmnis was 1 1.0 days at 23"C, 10.0 at26"C, 6.7 days at 29
C and 5.6 days at 32
C. Nymphal duration of C. 1i1~idiperzni.ws as 13.7 days at 23"C,12.7 days at 26
C. 10.2 days at 29
C and 9.1 days at 32
C regardless of food sources. Nymphaldevelopment was the shortest at 4th instar and the longest at 1st instar irrespective of temperatures and foodsources. Adult longevity of C. livirlipennis was was about 22.0 to 23.5 days at 23
C. 19.0 to 20.0 days at26"C, 16.0 to 17.0 days at 2Y0C, and 1 1.0 to 12.0 days at 32
C. There was no significant difference in adultlongevities on food sources. Number of eggs comsumed by adult C. lividipennis were about 56 to 61 and 56to 57,56 to 60 and 47 to 49,43 to 46 and 40 to 42, and 28 to 30 and 26 to 27 at 23'C. 20
C and 32"C,respectively. Egg consumption by adult C. lividiprrznis was slightly higher at female and on N. lugens eggthan at male and on L. striatellus egg. Dail 2.0 to 3.0 eggs were consumed by adult C. lividipc,nni.s.ail 2.0 to 3.0 eggs were consumed by adult C. lividipc,nni.s.
Larval Development of Mulberry Longicorn Beetle, Apriona germari Hope, on the Artificial Diet
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 317~322
Hatched-larvae of the mulberry longicorn beetle, Apriona germari Hope, collected from mulberry fields were reared on artificial diet at 25
C with 14 h light and 10 h dark to study the larval developmental characteristics. Artificial diet developed for rearing silkworm was used with minor modification adding mulberry branch powder. In case of artificial diet rearing, the head width of larval instar from the I st to the 12th instars was ranged from 0.12 to 0.69cm, and growth rate of each instar was significantly high between the I st and the 2nd instars. In addition, the weight of the 8th instar larvae was increased approximately 176-fold in comparision with that of the 1st instar larvae. Larval duration of each instar took long with larval developmental stages, and that of the 1st to the 9th or the 12th instars was 186.03 or 304.58 days, respectively. The survival rate of larvae was 40.8% by the 8th instar. The pupation rate was approximately 32.4%. Furthermore, although pupation stage was broadly appeared from the 7th to the I lth instars, pupation was majorly observed at the 8th and the 9th instars.
Sexual Behavior and Sex Pheromone Gland of the Apple Leafminer, Phyllonorycter ringoniella(Lepidoptera: Gracillariidae)
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 323~330
The apple leafminer, Phyllonorycrer ringoniella (Lepidoptera: Gracillariidae), is the most important pest on apple leaves and is not easy to be controlled with insecticides because of leafmining habit of its larvae. This study was undertaken to develop an artificial diet for mass rearing of the moth, and to look at their reproductive behavior. For developing an artificial diet the problem of diet contamination with microorganisms and how to supply artificial diet to first instar larvae have to be solved first. Artificial oviposition trial was successful with shadowy bottomside of various papers sprayed with apple leaf extracts. The highest activity of both calling and mating was observed within 30 min. after the onset of photophase and 3- to 4-day -old adults showed the highest mating rate. A ring-shaped sex pheromone gland was found at the intersegmental membrane between the 8th and 9th abdominal segments of the female. (E, 2)-4,lOtetradecadienyl acetate was identified as the major component from the femall gland extracts by the gas chromatography (GC) and GC-mass spectrometry. Additionally it was thought that (a-10-tetradecenyl acetate could be the second component of their sex pheromone.
Molecular Cloning and Expression of the Novel Attacin-Like Antibacterial Protein Gene Isolated from the Bombyx mori
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 331~340
Hyalophora cecropia attacin-like antibacterial gene was isolated from Bombyx mori induced with nonpathogenic bacteria. It was expressed in Spodopfera frugiperda 9 (Sf9 cells using baculovirus expression vector system (BEVS), and examined its antibacterial activity. With a cDNA library constructed from fifthinstar B. mori injected with Escherichia coli(4 X IOhcellsllarva), differential screening was performed using naive and induced mRNA probes. BmInc6 clone was screened by partial nucleotide sequence and GenBank database analysis. A complete nucleotide sequence of Bmlnc6 cDNA was determined (GenBank, AF005384). Its insert size was 852 bp and had open reading frame that started translation at position 35 and stopped at 679. And its putative polyadenylational signal existed at 812 bp. The number of amino acid deduced from Bmlnc6 cDNA was 214 and hydropathy analysis showed that this peptide was hydrophilic. This peptide deduced by BmInc6 was named nuecin. When the nuecin gene was expressed in Sf9 cells using BEVS, about 950 bp of the transcripts was detected. In addition, SDS-PAGE analysis showed that the molecular weights of intracellular expressed protein and the mature protein secreted to culture media were approximately 23 and 20 kDa, respectively. The antibacterial activity of nuecin against E. coli and Bacillus subtilis was significantly high, demonstrating that nuecin had a wider antibacterial spectrum with gram negative and positive bacteria than attacin. KEY WORDS Bombyx mori, antibacterial protein, attacin,
Expression of Fusion Protein with Autographa californica Nuclear Polyhedrosis Virus Polyhedrin and Bacillus thuringiensis cryIA(c) Crystal Protein in Insect Cells
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 341~350
We have now constructed a novel recombinant baculovirus producing fusion protein with Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin and Bacillus thuringiensis(Bt) cryIA(c) crystal protein. The fusion protein expressed by the recombinant baculovirus in insect cells was characterized. The N-terminal of cryIA(c) gene of Bt subsp. kurstaki HD-73 was introduced under the control of polyhedrin gene promoter of AcNPV, by fusion in the front of intact polyhedrin gene or by insertion into the HindIII site in polyhedrin gene. The recombinant baculoviruses were named as BtrusI or BtrusII, respectively. Although single transcript from the fusion protein gene was apparently observed. BtrusI was produced the two proteins, 92 kDa fusion protein and only polyhedrin. In addition, fusion protein produced by BtrusI did not form polyhedra. Interestingly, however, the cells infected with BtrusII did not show a 33 kDa polyhedrin band as a cells infected with BtrusI. Cells infected with BtrusII were only produced fusion protein, but the polyhedra formed by fusion protein was not observed. To determine the insecticidal toxicity of fusion protein, therefore, Sf9 cells infected with BtrusI were inoculated to Bombyx mori larvae. Sf9 cells infected with BtrusI that expressed the fusion protein caused larval mortality although the insecticidal toxicity was low. In conclusion, our results clearly demonstrated that the fusion protein with polyhedrin and Bt cryIA(c) crystal protein have a insecticida toxicity.
Pathogenicities of Beauveria bassiana GY1-17 against Some Agro-forest Insect Pests
Korean journal of applied entomology, volume 36, issue 4, 1997, Pages 351~356
Biological control of forest insect pests, Agelastica coeruleci, Meganola n~elancholia,a nd Glyphodes perspectalis, turfgrass insect pest, Blitoperfhu orientr~lis,v egetable insect pests, Plutella xylostella and Agrotis segetun~ with entomopathogenic fungus, Beciuveria hassinna GY 1 - 17 isolated from rice paddy of Yangsan in the southern part of Korea were investigated. Mortalities of A. coeruleu and P. ~ylo.~rellluar vae were 100% at the rate of 7.0 and 2.0 x 107conidia/ml after 7 and 5 days and those of M. melancholia were 66.7 - 100% at the rate of 0.03875-3.1 X 107conidia/ml. However, G. perspectulis was not affected at the rate of 2.0 x lo7 to X I04conidia/ml. And mortalites of B. orientcilis and A. segetum larvae were 46.7% at 3.7 x 107conidia/ml and 63.3% at 2.5 X 107conidia/ml.