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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean journal of applied entomology
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Journal DOI :
Korean Society of Applied Entomology
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Volume & Issues
Volume 38, Issue 3 - Dec 1999
Volume 38, Issue 2 - Aug 1999
Volume 38, Issue 1 - Apr 1999
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Notes on a Linden Leaf Mining Sawfly, Parna kamijoi(Hymenoptera, Tenthredinidae), New to Korea with Brief Life History Notes
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 81~84
A linden leaf-mining sawfly, Parna kamijoi Togashi, 1980, is newly reported from Korea. A brief description of the adult and adult and observations on its life history are given.
Biological Studies on Torymus sinensis Kamijo (Hymenoptera, Torymidae), a Parasitoid of Chestnut Gall Wasp Dryocosmus Kuriphilus Yasumatsu(Hymenoptera, Cynipidae)
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 85~91
Torymus sinensis was found to be a solitary ectoparasitoid attacking the larvae ofDryocosmus kuriphilus. The sex ratio of Torymus sinensis was close to 1 : 1 in foreign chestnut varieties.Under constant temperatures of 15"C, 20
C, and 25"C, the preoviposition period and longevity of theadult was longer at lower temperatures when fed on honey. Most of the Torymus sinensis female havemature eggs in their ovaries just after emergence. The number of mature eggs reached to average 22.5individuals per female in four days, which was the highest value, after the emergence and then decreasedgradually. Eggs were laid on the larvae of the host or attached on inner surface of the gall chamber. Theeggs were oval in shape, measuring 0.49 mm length and 0.13 mm width and the color of the justoviposited egg was milky white. The larvae appeared in one day after being deposited at 20
C. Thepupae, 2.25 mm length for female and 1.89 mm length for male, was milky white in color just afterpupation but became black later. In Kangwon Province, development period of chestnut galls formated byDryocosmus kuriphilus were mid April to late May and its primary parasitoid, Torymus sinensis adult,emerged from withered previous year galls in early April to early May. This species has one generation ina year, and hibernated with the matured larvae. hibernated with the matured larvae.
Effects of Photoperiod on Oviposition and Emergence of Egg Parasitoid, Trichogramma dendrolimi Mastumura (Hymenoptera, Trichogrammatidae)
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 93~99
We investigated the effect of constant photoperiod (16L : 8D, 12L : 12D, 8L : 16D), on the oviposition, parasitism, and emergence of Trichogramma dendrolimi. Also during 16L : 8D condition circadian, oviposition and emergence of this species on artificial eggs were checked every 2hrs. When twelve eggs of Antheraea pern~i and artificial eggs were provided, the wasps had the highest rate of oviposition in the first day, and decreased dramatically there after. In these different photoperiod, the total oviposition numbers per A. pernyi eggs by the wasp were 161.8 in 16L : 8D, 145.8 in 121 : 12D and 128.5 in 8L : 16D respectively in the first day. On the other hand for the artificial egg in 16L : 8D photoperiod, the total oviposition number per egg was 106.8 in the first day, 26.6 in the 2nd day and 21.3 in the 3rd day. The rate of emergence from A. pernyi eggs was not different depend on wasp's day age, however in the artificial eggs, after the first day's 84.7% decreased considerably. The emergence rate from artificial eggs was the highest comparatively between 04:OO-0600 hours.
Ecological Characteristics of Bombus ignitus Smith in Korea
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 101~107
Queens of Korean native bumblebee species, Bombus ignitus were collected from the field in the spring of 1998 to investigate their ecological characteristics and reared under the laboratory conditions; 3 0 f 1
C, 55 25% of relative humidity and darkness. As a result, 94.4% of the queens laid eggs in 6.00f 2.41 days after the field collection. The first worker and the first drone from the egg cell of the collected queens appeared in 18.90 f 1.16 and 68.96 f 3.94 days, respectively. In the colony foundation, 86.1% of the collected queens founded their colony, and 96.8% of them produced new queens in 71.14f 6.88 days. The life span of the colony-founded queen covered for 86.90f24.35 days, whereas that of non-colonized queen for 15.67 f 7.37 days. One colony sized as big as 987.82f 145.84 in number, composing of workers (188.79f 18.23), drones (691.10f 159.24) and queens (107.93 f47.48). Copulation lasting time of queen and drone took 23.00f 7.33 minutes in average. In a new queen emergence, there were two patterns; single peak type of new queen emergence in 12.31 k6.95 days and two peaks type of it in 74.00f 17.61 days. The number of eggs per egg cell of new queen averaged as 8.86f 4.00 grains and an egg period covered 3 days after oviposition. An egg cell sized as 7.15 f0.88 mm in a width, 7.79 f 1 .l 1 mm in a length and 3.33 f 0.23 mm in a height. An egg shaped as banana and sized as 1.22 f 0.09 mm in a width and as 3.5 1 f 0.21 mm in a length, weighing as 2.70 + 0.30 mg.
Morphological Characteristics and Life cycle of Udea ferrugalis (Lepidoptera, Pyralidae) Attacking Angelica utitis
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 109~115
Morphological characteristics and life cycle of Udea ferrugalis (Hubner) attacking Angelica utilis were studied in 1995 and 1996. Egg of U. ferrugalis was oval-shaped with light-gray. Larva was milk white or light yellow color with 1.50-14.6 mm body length, depending on their developmental stages from 1st to 5th instar. Pupa were deep-brown with 8.2-9.7 mm length. Adults were brown color and body lengths were 10.1 mm and 8.3 mm and wing expanse lengths were 18.5 mm and 16.6mm with female and male, respectively. At 20
C, each developmently periods was 5.81, 19.0, 3.46 and 1 l.0days with egg, larvae, pre-pupae, and pupae, respectively. Also adult longevitys were 10.13 for female and 9.8 days for male. Average number of egg were 238.5 and 248.8 at 20
C and 30
C, respectively. A total of 12 species host plants were identified in the outdoor observation in Chungbuk province.
Effects of Temperatures on Development and Reproduction of the Sycamore Lace Bug, Corythucha ciliata (Hemiptera, Tingidae)
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 117~121
Development and reproduction of the sycamore lace bug, Corythucha ciliata, were investigated under different temperature regimes. Duration of development from egg to pre-adult of the sycamore lace bug measured seven temperatures ranged from 54.0 days at 18
C to 17.9 days at 33
C. Development was not successful at 15
C and 35
C. Developmental zero point and total effective temperature for development of egg, nymphal, and complete development were 1 1 .O, 10.9, ll.l
C and 150.3, 230.6, 376.1 degree-days, respectively. Longevities of adult females varied to temperature from 51.8 days at 18
C to 17.2 days at 33
C. The average fecundity per female was greater at 25
C and 28
C compared with at other temperatures. The intrinsic rate of natural increase (r,) and net reproduction rate (R,) were highest at 28
C as 0.170 and 73.25, respectively. As a result, optimum ranges of temperature for C. ciliata growth were between 25
C and 28
Characteristics of the Oxygen Uptake Rate of Entomopathogenic Nematodes Steinernema spp.
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 123~128
Recently, entomopathogenic nematodes have received a considerable attention as biologicalcontrol agents. For in vitro cultivation, storage and transportation of nematodes, oxygen supply isextremely impotant due to its limited solubility and mass transfer problem. The oxygen uptake rates(OURs) of four different Steinernema species were measured in a 5L bioreactor at varying temperatures.The OURs of the Steinernema spp. were below 0.5 x mmolO'||'&'||' . min in the range of 13-17
C. TheOURs (mmo102/L - min) of S. glaseri Dongrae and S. carpocapsae Pocheon strains were 0.4 x lo-', 0.75x lo-\ulcorner at 21
C, 1.5 x lo-\ulcorner, 3.2 x 10-2 at 25"C, and 2.8 x lo-', 5.8 x lo-\ulcorner at 29"C, respectively. However,the OURs were not significantly altered by the agitation speed of 50-150 rpm. The specific oxygenuptake rates (qol) of S. glaseri NC, S. glaseri Dongrae, S. glaseri Mungyeong and S. carpocapsaePocheon strains were 0.3 x 0.5 x 0.3 x and 0.2 x mmolO~/cell min at 25"C,respectively. As the nematode size and temperature were increased, the qo, was also increased.the qo, was also increased.
Age-Related Ultrastructural Changes in Fat Body of Silkworm Adult, Bombyx mori
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 129~138
This study was carried out with two types of silkworm, Bombyx mori, adults having different life span: long life span (adult living for more than fifteen days; LLS), and short life span (adult living less for than five days; SLS). The fine structures of fat body cells in the young and the senescent males and females were compared in an attempt to elucidate the aging physiology of the silkworm adult. In SLS, there were many rough endoplasmic reticula (RER) and glycogen granules in the female cytoplasm, whereas the smooth endoplasmic reticula (SER) were observed in the male cytoplasm. Also in SLS, the mitochondria were swollen and had many poor osmiophillic inclusions (fatty degeneration) at three days after emergence. In LLS, compared to SLS, comparatively normal mitochondria and nuclear membrane were observed in even five day old female. In fifteen day old LLS female, however most of cell membranes were disappeared and mitochondria were abnormally expanded. Many fat granules were observed in the cytoplasm of ten day old male (LLS) which died with full autophagic vacuole (AV) collapsed at fifteen days. Therefore, it is suggested that SLS type is recommended for the study of aging tissue, because it is easier to monitor the changes in tissue structure.
Expression and Characterization of Fusion Protein with Autographa californica Nuclear Polyhedrosis Virus Polyhedrin and Green Fluorescent Protein in Insect Cells
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 139~144
We have now constructed a novel recombinant baculovirus producing fusion protein with Autogrqha c.uliforrzica nuclear polyhedrosis virus (AcNPV) polyhedrin and green fluorescent protein (GFP). The fusion protein expressed by the recombinant baculovirus in insect cells was characterized. The GFP gene was introduced under the control of polyhedrin gene promoter of AcNPV, by fusion in the front or back of intact polyhedrin gene. The recombinant baculoviruses were named as Ac-GFPPOL or Ac-POLGFP. respectively. As expected, the 56 kDa fusion protein was expressed in the recombinant virus-infected cells. Interestingly. however, the fluorescence of GFP in the cells infected with Ac- POLGFP was only detected within the nuclei. and that was observed as polyhedra-like granular particles. In the microscopy of cells infected with Ac-GFPPOL, furthermore, GFP was detected in both cytoplasm and nuclei although most of GFP were present within the nuclei. However, fusion protein produced by recombinant virus did not form polyhedra although the fusion protein was fused with polyhedrin and GFP. It is suggested that difference of GFP location in the infected cells appear to be involved in the region of polyhedrin in the fusion protein, and the polyhedrin in the fusion protein might be responsible for the polyhedra-like granular particles present within nuclei.
Structure of Spodoptera exigua Nucleopolyhedrovirus p10 Gene
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 145~149
To develop the baculovirus expression vector system (BEVS) adopting p10 gene promoter of Spodoptera exigua nucleopolyhedrovirus (SeNPV), we characterized the p10 gene of SeNPV. The nucleotide sequence of 545 bases including the coding region of p10 gene was determined. Compared with the previously reported SeNPV p10 gene (Zuidema et al., 1993), 4 bases were different in the 5' and 3' flanking region but no difference was found in the coding region. The p10 gene was located within Xho I 1.5 Kb, Sph 1 2.4 Kb and Cla I 4.0 Kb fragments by Southern hybridization analysis. Also, the Sph I 2.4 Kb and the Cla I 4.0 Kb fragments were cloned and their restriction enzyme maps were determined.
A Preliminary Study on the Biological Control of Tetranychus kanzawai Kishida in Angelica utlis Makino by Phytoseiulus persimilis Anthias-Henriot (Acarina: Tetranychidae, Phytoseiidae)
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 151~155
Biological control of Tetranychus kanzawai by Phytoseiulus persimilis on the Angelica utilis was done in 'walk-in' plastic tunnels. The population of T. kanzawai nymphs and adults per 4 cm2 in damaged leaf decreased from 25 mites on July 22 after 25 predatory mites per m2 were released on July 23 to 0.4 mites on September 9 and then rose to 9.3 mites on October 16, 1997. Phytoseiulus persimilis could suppress T. kanzawai to low population level from August 13 to October 1.
A New Record of Tarsonemid Mite, Stenotarsonemus spinki (Acari, Tarsonemidae) and Its Damage on Rice in Korea
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 157~164
A tarsonemid mite. Stet~rotc~r.sot~rrrs~puin.sk i Smiley 1967. is an important rice pest in major rice producing countries of Thailand. Philippine. China, and Taiwan. S. spinki was found first time in Korea from rice grown in environment controlled greenhouse. Rice fed by the mite showed damage symptoms of deformed panicles and inflorescence, lesions on the inner surface of leaf sheath, and browning of rice hulls. Females of S. .spi~lkwi as 263.0 pni (246.5-284.6f 12.2) in body length and 92.4 pm (79.5- 104.9 t 7.6) in body width. Body was elongate and broadest in region of hysterosoma. Body color was pale brown. Legs were robust except for the legs IV which were typical tarsonemid female legs terminating in a whiplike seta two times the length of the leg. Male of S. spir~kiw as 196.5 pm ( 176.5-222.8 + 15.8) in body length and 109.3 ym (98.6- 1 17.7 + 6.4) in body width. Anterior ends of apodemes 111 were extended further than apodemes IV. Femur IV had large inner median lateral flange. and inner anterior and outer median setae were short about equal length. Tarsal claw was stout and curved ventrally.
Susceptibility of Medically Important Mosquito Larvae and Larvivorous Fishes to Abate and Abate-S in Korea
Korean journal of applied entomology, volume 38, issue 2, 1999, Pages 165~169
A study was performed on the susceptibility of Abate@ and Abate-S@ against 3rd instar larvae of 5 species of mosquitoes including Anopheles sinensis, Aedes dorsalis, Culex inatomii, Cx. pipiens pallens, Cx. tritaeniorhynchus and mosquito larvivorous fishes such as Chinese muddy loaches (Misgurnus muzolepis) and crucian carps (Carassius carassi~4s) in September, 1998. Cx. pipiens larvae showed that the LC5os of both Abatea and Abate-Sa were 0.006 ppm. The LC95 values of this mosquito species appeared 0.070 pprn of Abate@ and 0.035 pprn of Abate-S@ which were more susceptible than the dosage (1.0 ppm) recommended from WHO. An. sinensis larvae appeared 0.009 pprn of LC50 and 0.025 pprn of LCgs. The larvae of Cx. inatomii and Ae. dorsalis showed the most highly susceptible to Abate-S@ among the 5 mosquito species with LC50 values of 1.9 x pprn and 1.1 x pprn respectively, and the LC95 values were 5.2 x 10-hppm and 1.4 x ppm, respectively. On the other hand, Cx. tritaeniorhynchus appeared least affected by Abate-Sa. and was brought 0.048 pprn of LC50 and 0.808 pprn of LCg5. These two insecticides were much less toxic to the fishes than mosquito larvae showing the LC, value of Chinese muddy loaches at 24.145 pprn of Abate and 10.750ppm of Abate-S@. This fish species showed that the LCsos of Abate@ and Abate-S@ were 27.567 pprn and 14.775 ppm, respectively. In case of crucian carps, their LC, values were 7.914 pprn of Abate@ and 6.480 pprn of Abate-S@. Therefore, the maximum safe dosages of the insecticides to the fishes were 8 times as high as the values of LCg5 of the mosquito larvae. This study suggests that Abate@ and Abate-S@ demonstrated highly potential insecticides for further larger scale operational integrated control in some proper aquatic places if other aquatic invertebrates are also safe to the insecticides.